The use of enzymatic preparation for the production of low molecular-weight chitosan from the king crab hepatopancrease
This article describes the optimal conditions for the enzymatic hydrolysis of chitosan and its chemically-modified derivatives using the preparation extracted from the king crab hepatopancrease possessing pronounced hydrolythic activity. The following preparations were used: chitosan with a molecula...
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| Veröffentlicht in: | Applied biochemistry and microbiology 2009-07, Vol.45 (4), p.374-379 |
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| container_title | Applied biochemistry and microbiology |
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| creator | Ilyina, A. V. Zagorskaya, D. S. Levov, A. N. Albulov, A. I. Kovacheva, N. P. Varlamov, V. P. |
| description | This article describes the optimal conditions for the enzymatic hydrolysis of chitosan and its chemically-modified derivatives using the preparation extracted from the king crab hepatopancrease possessing pronounced hydrolythic activity. The following preparations were used: chitosan with a molecular weight of 700 kDa and an acetylation level of 0.15, carboxymethyl chitosan 200 kDa witih an extent of replacement of 0.23, and N-succinyl chitosan 390 kDa with an extent of replacement of 0.8. Low molecular-weight samples of chitosan and of its modified derivatives were obtained with the yields of 85, 55, and 80%, respectively. The conditions of the hydrolysis were as follows: an enzyme: substrate ratio of 1: 200, 37°C, and 20 h duration of hydrolysis. |
| doi_str_mv | 10.1134/S0003683809040048 |
| format | Article |
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V. ; Zagorskaya, D. S. ; Levov, A. N. ; Albulov, A. I. ; Kovacheva, N. P. ; Varlamov, V. P.</creator><creatorcontrib>Ilyina, A. V. ; Zagorskaya, D. S. ; Levov, A. N. ; Albulov, A. I. ; Kovacheva, N. P. ; Varlamov, V. P.</creatorcontrib><description>This article describes the optimal conditions for the enzymatic hydrolysis of chitosan and its chemically-modified derivatives using the preparation extracted from the king crab hepatopancrease possessing pronounced hydrolythic activity. The following preparations were used: chitosan with a molecular weight of 700 kDa and an acetylation level of 0.15, carboxymethyl chitosan 200 kDa witih an extent of replacement of 0.23, and N-succinyl chitosan 390 kDa with an extent of replacement of 0.8. Low molecular-weight samples of chitosan and of its modified derivatives were obtained with the yields of 85, 55, and 80%, respectively. The conditions of the hydrolysis were as follows: an enzyme: substrate ratio of 1: 200, 37°C, and 20 h duration of hydrolysis.</description><identifier>ISSN: 0003-6838</identifier><identifier>EISSN: 1608-3024</identifier><identifier>DOI: 10.1134/S0003683809040048</identifier><language>eng</language><publisher>Dordrecht: SP MAIK Nauka/Interperiodica</publisher><subject>Biochemistry ; Bioengineering ; Biomedical and Life Sciences ; Crustaceans ; Decapoda ; Enzymes ; Hydrolysis ; Life Sciences ; Marine ; Medical Microbiology ; Microbiology ; Molecular weight</subject><ispartof>Applied biochemistry and microbiology, 2009-07, Vol.45 (4), p.374-379</ispartof><rights>Pleiades Publishing, Ltd. 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c346t-6829b8ef4bdc50db2f509463b8712bb61ac24b50380dad55c15658e94a4f8ec3</citedby><cites>FETCH-LOGICAL-c346t-6829b8ef4bdc50db2f509463b8712bb61ac24b50380dad55c15658e94a4f8ec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1134/S0003683809040048$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1134/S0003683809040048$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids></links><search><creatorcontrib>Ilyina, A. 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| subjects | Biochemistry Bioengineering Biomedical and Life Sciences Crustaceans Decapoda Enzymes Hydrolysis Life Sciences Marine Medical Microbiology Microbiology Molecular weight |
| title | The use of enzymatic preparation for the production of low molecular-weight chitosan from the king crab hepatopancrease |
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