Low level of polymyxin resistance among nonclonal mcr-1–positive Escherichia coli from human sources in Brazil
We report 26 human isolates of mcr-1–positive Escherichia coli, most of them (65.4%) with a polymyxin B MIC of 2 mg/L. Seventeen out of the 24 mcr-1–positive E. coli proved to be nonclonal by rep-PCR which strengthens the hypothesis of environmental or animal origin of these strains and reinforces t...
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| Veröffentlicht in: | Diagnostic microbiology and infectious disease 2019-02, Vol.93 (2), p.140-142 |
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| container_title | Diagnostic microbiology and infectious disease |
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| creator | Pillonetto, Marcelo Mazzetti, Alana Becker, Guilherme N. Siebra, Christian A. Arend, Lavinia N.V.S Barth, Afonso L. |
| description | We report 26 human isolates of mcr-1–positive Escherichia coli, most of them (65.4%) with a polymyxin B MIC of 2 mg/L. Seventeen out of the 24 mcr-1–positive E. coli proved to be nonclonal by rep-PCR which strengthens the hypothesis of environmental or animal origin of these strains and reinforces the one health context of antimicrobial resistance.
•The mcr-1 gene was detected in 26/368 (7.1%) Escherichia coli isolates from 19 patients.•None of the mcr-1–positive isolates were positive for blaKPC or blaNDM genes.•A total of 65.4% of mcr-1–positive isolates were susceptible to polymyxin B.•Most mcr-1–positive strains (18/26) exhibited a positive result for ESBL.•Molecular typing of 24/26 MCR-1–Escherichia coli indicated no clonal relation. |
| doi_str_mv | 10.1016/j.diagmicrobio.2018.08.009 |
| format | Article |
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•The mcr-1 gene was detected in 26/368 (7.1%) Escherichia coli isolates from 19 patients.•None of the mcr-1–positive isolates were positive for blaKPC or blaNDM genes.•A total of 65.4% of mcr-1–positive isolates were susceptible to polymyxin B.•Most mcr-1–positive strains (18/26) exhibited a positive result for ESBL.•Molecular typing of 24/26 MCR-1–Escherichia coli indicated no clonal relation.</description><identifier>ISSN: 0732-8893</identifier><identifier>EISSN: 1879-0070</identifier><identifier>DOI: 10.1016/j.diagmicrobio.2018.08.009</identifier><identifier>PMID: 30355469</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Anti-Bacterial Agents - pharmacology ; Antimicrobial resistance ; Cohort Studies ; Drug Resistance, Bacterial - genetics ; E. coli ; Escherichia coli - drug effects ; Escherichia coli - genetics ; Escherichia coli Infections - microbiology ; Escherichia coli Proteins - genetics ; Humans ; mcr-1 ; Microbial Sensitivity Tests ; One health ; Polymyxin resistance ; Polymyxins - pharmacology</subject><ispartof>Diagnostic microbiology and infectious disease, 2019-02, Vol.93 (2), p.140-142</ispartof><rights>2018 Elsevier Inc.</rights><rights>Copyright © 2018 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-b0c835f7d6a932ce5760378a37df8def822986dfbdcabeb5172a255bef0645593</citedby><cites>FETCH-LOGICAL-c380t-b0c835f7d6a932ce5760378a37df8def822986dfbdcabeb5172a255bef0645593</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.diagmicrobio.2018.08.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30355469$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pillonetto, Marcelo</creatorcontrib><creatorcontrib>Mazzetti, Alana</creatorcontrib><creatorcontrib>Becker, Guilherme N.</creatorcontrib><creatorcontrib>Siebra, Christian A.</creatorcontrib><creatorcontrib>Arend, Lavinia N.V.S</creatorcontrib><creatorcontrib>Barth, Afonso L.</creatorcontrib><title>Low level of polymyxin resistance among nonclonal mcr-1–positive Escherichia coli from human sources in Brazil</title><title>Diagnostic microbiology and infectious disease</title><addtitle>Diagn Microbiol Infect Dis</addtitle><description>We report 26 human isolates of mcr-1–positive Escherichia coli, most of them (65.4%) with a polymyxin B MIC of 2 mg/L. Seventeen out of the 24 mcr-1–positive E. coli proved to be nonclonal by rep-PCR which strengthens the hypothesis of environmental or animal origin of these strains and reinforces the one health context of antimicrobial resistance.
•The mcr-1 gene was detected in 26/368 (7.1%) Escherichia coli isolates from 19 patients.•None of the mcr-1–positive isolates were positive for blaKPC or blaNDM genes.•A total of 65.4% of mcr-1–positive isolates were susceptible to polymyxin B.•Most mcr-1–positive strains (18/26) exhibited a positive result for ESBL.•Molecular typing of 24/26 MCR-1–Escherichia coli indicated no clonal relation.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antimicrobial resistance</subject><subject>Cohort Studies</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>E. coli</subject><subject>Escherichia coli - drug effects</subject><subject>Escherichia coli - genetics</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Humans</subject><subject>mcr-1</subject><subject>Microbial Sensitivity Tests</subject><subject>One health</subject><subject>Polymyxin resistance</subject><subject>Polymyxins - pharmacology</subject><issn>0732-8893</issn><issn>1879-0070</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkMGO0zAQhi0EYsvCKyCLE5eUsV3HNjdYdgGpEhc4W44z2bpy4mAnhXLiHXjDfRJSdUEckUaayzf_r_kIecFgzYDVr_brNrjbPvicmpDWHJhewzJgHpAV08pUAAoekhUowSutjbggT0rZAzBuNvCYXAgQUm5qsyLjNn2jEQ8YaeromOKxP34PA81YQpnc4JG6Pg23dEiDj2lwkfY-V-zu568xlTCFA9Lr4neYg98FR32KgXY59XQ3926gJc3ZY6FL5NvsfoT4lDzqXCz47H5fki8315-vPlTbT-8_Xr3ZVl5omKoGvBayU23tjOAepapBKO2EajvdYqc5N7puu6b1rsFGMsUdl7LBDuqNlEZckpfn3DGnrzOWyfaheIzRDZjmYjnjUoAyTC3o6zO6CC0lY2fHHHqXj5aBPRm3e_uvcXsybmEZOPU8v--Zmx7bv6d_FC_AuzOAy7eHgNkWH3AR24aMfrJtCv_T8xsQV5wq</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Pillonetto, Marcelo</creator><creator>Mazzetti, Alana</creator><creator>Becker, Guilherme N.</creator><creator>Siebra, Christian A.</creator><creator>Arend, Lavinia N.V.S</creator><creator>Barth, Afonso L.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201902</creationdate><title>Low level of polymyxin resistance among nonclonal mcr-1–positive Escherichia coli from human sources in Brazil</title><author>Pillonetto, Marcelo ; Mazzetti, Alana ; Becker, Guilherme N. ; Siebra, Christian A. ; Arend, Lavinia N.V.S ; Barth, Afonso L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-b0c835f7d6a932ce5760378a37df8def822986dfbdcabeb5172a255bef0645593</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antimicrobial resistance</topic><topic>Cohort Studies</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>E. coli</topic><topic>Escherichia coli - drug effects</topic><topic>Escherichia coli - genetics</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Humans</topic><topic>mcr-1</topic><topic>Microbial Sensitivity Tests</topic><topic>One health</topic><topic>Polymyxin resistance</topic><topic>Polymyxins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pillonetto, Marcelo</creatorcontrib><creatorcontrib>Mazzetti, Alana</creatorcontrib><creatorcontrib>Becker, Guilherme N.</creatorcontrib><creatorcontrib>Siebra, Christian A.</creatorcontrib><creatorcontrib>Arend, Lavinia N.V.S</creatorcontrib><creatorcontrib>Barth, Afonso L.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic microbiology and infectious disease</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pillonetto, Marcelo</au><au>Mazzetti, Alana</au><au>Becker, Guilherme N.</au><au>Siebra, Christian A.</au><au>Arend, Lavinia N.V.S</au><au>Barth, Afonso L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low level of polymyxin resistance among nonclonal mcr-1–positive Escherichia coli from human sources in Brazil</atitle><jtitle>Diagnostic microbiology and infectious disease</jtitle><addtitle>Diagn Microbiol Infect Dis</addtitle><date>2019-02</date><risdate>2019</risdate><volume>93</volume><issue>2</issue><spage>140</spage><epage>142</epage><pages>140-142</pages><issn>0732-8893</issn><eissn>1879-0070</eissn><abstract>We report 26 human isolates of mcr-1–positive Escherichia coli, most of them (65.4%) with a polymyxin B MIC of 2 mg/L. Seventeen out of the 24 mcr-1–positive E. coli proved to be nonclonal by rep-PCR which strengthens the hypothesis of environmental or animal origin of these strains and reinforces the one health context of antimicrobial resistance.
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Anti-Bacterial Agents - pharmacology Antimicrobial resistance Cohort Studies Drug Resistance, Bacterial - genetics E. coli Escherichia coli - drug effects Escherichia coli - genetics Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics Humans mcr-1 Microbial Sensitivity Tests One health Polymyxin resistance Polymyxins - pharmacology |
| title | Low level of polymyxin resistance among nonclonal mcr-1–positive Escherichia coli from human sources in Brazil |
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