Replicon typing of plasmids in environmental Achromobacter sp. producing quinolone‐resistant determinants
This study aimed to investigate the antimicrobial resistance profile to quinolones, the presence of quinolone‐resistant determinants and the plasmid replicon typing in environmental Achromobacter sp. isolated from Brazil. Soil and water samples were used for bacterial isolation. The antimicrobial su...
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| Veröffentlicht in: | APMIS : acta pathologica, microbiologica et immunologica Scandinavica microbiologica et immunologica Scandinavica, 2018-11, Vol.126 (11), p.864-869 |
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| container_title | APMIS : acta pathologica, microbiologica et immunologica Scandinavica |
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| creator | Furlan, João Pedro Rueda Sanchez, Danilo Garcia Gallo, Inara Fernanda Lage Stehling, Eliana Guedes |
| description | This study aimed to investigate the antimicrobial resistance profile to quinolones, the presence of quinolone‐resistant determinants and the plasmid replicon typing in environmental Achromobacter sp. isolated from Brazil. Soil and water samples were used for bacterial isolation. The antimicrobial susceptibility testing was performed by minimum inhibitory concentration method. The detection of mutations in the quinolone resistance‐determining regions (QRDR) genes, the presence of plasmid‐mediated quinolone resistance (PMQR) genes, and plasmid replicons were performed by PCR. A total of 16 isolates was obtained from different cultures, cities, and states of Brazil. All isolates were non‐susceptible to ciprofloxacin, norfloxacin, and levofloxacin. Some mutations in QRDR genes were found, including Gln‐83‐Leu and Asp‐87‐Asn in the gyrA and Gln‐80‐Ile and Asp‐84‐Ala in the parC. Different PMQR genes were detected, such as qnrA, qnrB, qnrS, oqxA, and oqxB. Three different plasmid families were detected, being most presented the ColE‐like, followed by IncFIB and IncA/C. The presence of different PMQR genes and plasmids in the isolates of the present study shows that environmental bacteria can act as reservoir of important genes of resistance to fluoroquinolones, which is of great concern, due to the potential of horizontal dissemination of these genes. Besides that, there are no studies reporting these results in Achromobacter sp. isolates. |
| doi_str_mv | 10.1111/apm.12896 |
| format | Article |
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Soil and water samples were used for bacterial isolation. The antimicrobial susceptibility testing was performed by minimum inhibitory concentration method. The detection of mutations in the quinolone resistance‐determining regions (QRDR) genes, the presence of plasmid‐mediated quinolone resistance (PMQR) genes, and plasmid replicons were performed by PCR. A total of 16 isolates was obtained from different cultures, cities, and states of Brazil. All isolates were non‐susceptible to ciprofloxacin, norfloxacin, and levofloxacin. Some mutations in QRDR genes were found, including Gln‐83‐Leu and Asp‐87‐Asn in the gyrA and Gln‐80‐Ile and Asp‐84‐Ala in the parC. Different PMQR genes were detected, such as qnrA, qnrB, qnrS, oqxA, and oqxB. Three different plasmid families were detected, being most presented the ColE‐like, followed by IncFIB and IncA/C. The presence of different PMQR genes and plasmids in the isolates of the present study shows that environmental bacteria can act as reservoir of important genes of resistance to fluoroquinolones, which is of great concern, due to the potential of horizontal dissemination of these genes. Besides that, there are no studies reporting these results in Achromobacter sp. isolates.</description><identifier>ISSN: 0903-4641</identifier><identifier>EISSN: 1600-0463</identifier><identifier>DOI: 10.1111/apm.12896</identifier><identifier>PMID: 30357960</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Achromobacter ; Achromobacter - drug effects ; Achromobacter - genetics ; Achromobacter - growth & development ; Achromobacter - metabolism ; Achromobacter sp ; Amino Acid Sequence ; Anti-Bacterial Agents - pharmacology ; Antiinfectives and antibacterials ; Antimicrobial agents ; Antimicrobial resistance ; Bacterial Typing Techniques - methods ; Brazil ; Ciprofloxacin ; Ciprofloxacin - pharmacology ; DNA Gyrase - genetics ; DNA Gyrase - metabolism ; DNA Topoisomerase IV - genetics ; DNA Topoisomerase IV - metabolism ; Drug Resistance, Bacterial - genetics ; environment ; Fluoroquinolones ; Gene Expression ; Genes ; Humans ; Levofloxacin ; Levofloxacin - pharmacology ; Microbial Sensitivity Tests ; Minimum inhibitory concentration ; Mutation ; Norfloxacin ; Norfloxacin - pharmacology ; plasmid ; Plasmids ; Plasmids - chemistry ; Plasmids - metabolism ; plasmid‐mediated quinolone resistance ; quinolone resistance‐determining regions ; Quinolones ; Replicon ; Soil Microbiology ; Soil water ; Typing ; Water analysis ; Water Microbiology ; Water sampling</subject><ispartof>APMIS : acta pathologica, microbiologica et immunologica Scandinavica, 2018-11, Vol.126 (11), p.864-869</ispartof><rights>2018 APMIS. Published by John Wiley & Sons Ltd</rights><rights>2018 APMIS. 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Soil and water samples were used for bacterial isolation. The antimicrobial susceptibility testing was performed by minimum inhibitory concentration method. The detection of mutations in the quinolone resistance‐determining regions (QRDR) genes, the presence of plasmid‐mediated quinolone resistance (PMQR) genes, and plasmid replicons were performed by PCR. A total of 16 isolates was obtained from different cultures, cities, and states of Brazil. All isolates were non‐susceptible to ciprofloxacin, norfloxacin, and levofloxacin. Some mutations in QRDR genes were found, including Gln‐83‐Leu and Asp‐87‐Asn in the gyrA and Gln‐80‐Ile and Asp‐84‐Ala in the parC. Different PMQR genes were detected, such as qnrA, qnrB, qnrS, oqxA, and oqxB. Three different plasmid families were detected, being most presented the ColE‐like, followed by IncFIB and IncA/C. The presence of different PMQR genes and plasmids in the isolates of the present study shows that environmental bacteria can act as reservoir of important genes of resistance to fluoroquinolones, which is of great concern, due to the potential of horizontal dissemination of these genes. Besides that, there are no studies reporting these results in Achromobacter sp. isolates.</description><subject>Achromobacter</subject><subject>Achromobacter - drug effects</subject><subject>Achromobacter - genetics</subject><subject>Achromobacter - growth & development</subject><subject>Achromobacter - metabolism</subject><subject>Achromobacter sp</subject><subject>Amino Acid Sequence</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antiinfectives and antibacterials</subject><subject>Antimicrobial agents</subject><subject>Antimicrobial resistance</subject><subject>Bacterial Typing Techniques - methods</subject><subject>Brazil</subject><subject>Ciprofloxacin</subject><subject>Ciprofloxacin - pharmacology</subject><subject>DNA Gyrase - genetics</subject><subject>DNA Gyrase - metabolism</subject><subject>DNA Topoisomerase IV - genetics</subject><subject>DNA Topoisomerase IV - metabolism</subject><subject>Drug Resistance, Bacterial - genetics</subject><subject>environment</subject><subject>Fluoroquinolones</subject><subject>Gene Expression</subject><subject>Genes</subject><subject>Humans</subject><subject>Levofloxacin</subject><subject>Levofloxacin - pharmacology</subject><subject>Microbial Sensitivity Tests</subject><subject>Minimum inhibitory concentration</subject><subject>Mutation</subject><subject>Norfloxacin</subject><subject>Norfloxacin - pharmacology</subject><subject>plasmid</subject><subject>Plasmids</subject><subject>Plasmids - chemistry</subject><subject>Plasmids - metabolism</subject><subject>plasmid‐mediated quinolone resistance</subject><subject>quinolone resistance‐determining regions</subject><subject>Quinolones</subject><subject>Replicon</subject><subject>Soil Microbiology</subject><subject>Soil water</subject><subject>Typing</subject><subject>Water analysis</subject><subject>Water Microbiology</subject><subject>Water sampling</subject><issn>0903-4641</issn><issn>1600-0463</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp10c9PwyAUB3BiNG5OD_4DhsSLHjqBUlaOy-KvZEZj9NxQSpXZQgetZjf_BP9G_xKZnR5M5AKHz_vmPR4AhxiNcThnoqnHmKScbYEhZghFiLJ4GwwRR3FEGcUDsOf9AqGA2GQXDGIUJxPO0BC83Kum0tIa2K4abZ6gLWFTCV_rwkNtoDKv2llTK9OKCk7ls7O1zYVslYO-GcPG2aKT68Jlp42trFGf7x9Oee1bYVpYqCBrbcLb74OdUlReHWzuEXi8OH-YXUXz28vr2XQeyTiJWZQqmZMkx7kUKpecMpnSJAljlDglpSBlQXAqc4TKBKVIYsoLShFNeSiipSTxCJz0uaG5Zad8m9XaS1VVwijb-YxgkhDOKWGBHv-hC9s5E7pbK8r4JOE4qNNeSWe9d6rMGqdr4VYZRtl6A1nYQPa9gWCPNoldXqviV_58eQBnPXjTlVr9n5RN7276yC8qk5Ih</recordid><startdate>201811</startdate><enddate>201811</enddate><creator>Furlan, João Pedro Rueda</creator><creator>Sanchez, Danilo Garcia</creator><creator>Gallo, Inara Fernanda Lage</creator><creator>Stehling, Eliana Guedes</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>201811</creationdate><title>Replicon typing of plasmids in environmental Achromobacter sp. producing quinolone‐resistant determinants</title><author>Furlan, João Pedro Rueda ; Sanchez, Danilo Garcia ; Gallo, Inara Fernanda Lage ; Stehling, Eliana Guedes</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3536-8ecb25b1bcaebc946c8455464f182fa2fd218cb00f5080c149d44048925b4fc23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Achromobacter</topic><topic>Achromobacter - drug effects</topic><topic>Achromobacter - genetics</topic><topic>Achromobacter - growth & development</topic><topic>Achromobacter - metabolism</topic><topic>Achromobacter sp</topic><topic>Amino Acid Sequence</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antiinfectives and antibacterials</topic><topic>Antimicrobial agents</topic><topic>Antimicrobial resistance</topic><topic>Bacterial Typing Techniques - methods</topic><topic>Brazil</topic><topic>Ciprofloxacin</topic><topic>Ciprofloxacin - pharmacology</topic><topic>DNA Gyrase - genetics</topic><topic>DNA Gyrase - metabolism</topic><topic>DNA Topoisomerase IV - genetics</topic><topic>DNA Topoisomerase IV - metabolism</topic><topic>Drug Resistance, Bacterial - genetics</topic><topic>environment</topic><topic>Fluoroquinolones</topic><topic>Gene Expression</topic><topic>Genes</topic><topic>Humans</topic><topic>Levofloxacin</topic><topic>Levofloxacin - pharmacology</topic><topic>Microbial Sensitivity Tests</topic><topic>Minimum inhibitory concentration</topic><topic>Mutation</topic><topic>Norfloxacin</topic><topic>Norfloxacin - pharmacology</topic><topic>plasmid</topic><topic>Plasmids</topic><topic>Plasmids - chemistry</topic><topic>Plasmids - metabolism</topic><topic>plasmid‐mediated quinolone resistance</topic><topic>quinolone resistance‐determining regions</topic><topic>Quinolones</topic><topic>Replicon</topic><topic>Soil Microbiology</topic><topic>Soil water</topic><topic>Typing</topic><topic>Water analysis</topic><topic>Water Microbiology</topic><topic>Water sampling</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Furlan, João Pedro Rueda</creatorcontrib><creatorcontrib>Sanchez, Danilo Garcia</creatorcontrib><creatorcontrib>Gallo, Inara Fernanda Lage</creatorcontrib><creatorcontrib>Stehling, Eliana Guedes</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Furlan, João Pedro Rueda</au><au>Sanchez, Danilo Garcia</au><au>Gallo, Inara Fernanda Lage</au><au>Stehling, Eliana Guedes</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Replicon typing of plasmids in environmental Achromobacter sp. producing quinolone‐resistant determinants</atitle><jtitle>APMIS : acta pathologica, microbiologica et immunologica Scandinavica</jtitle><addtitle>APMIS</addtitle><date>2018-11</date><risdate>2018</risdate><volume>126</volume><issue>11</issue><spage>864</spage><epage>869</epage><pages>864-869</pages><issn>0903-4641</issn><eissn>1600-0463</eissn><abstract>This study aimed to investigate the antimicrobial resistance profile to quinolones, the presence of quinolone‐resistant determinants and the plasmid replicon typing in environmental Achromobacter sp. isolated from Brazil. Soil and water samples were used for bacterial isolation. The antimicrobial susceptibility testing was performed by minimum inhibitory concentration method. The detection of mutations in the quinolone resistance‐determining regions (QRDR) genes, the presence of plasmid‐mediated quinolone resistance (PMQR) genes, and plasmid replicons were performed by PCR. A total of 16 isolates was obtained from different cultures, cities, and states of Brazil. All isolates were non‐susceptible to ciprofloxacin, norfloxacin, and levofloxacin. Some mutations in QRDR genes were found, including Gln‐83‐Leu and Asp‐87‐Asn in the gyrA and Gln‐80‐Ile and Asp‐84‐Ala in the parC. Different PMQR genes were detected, such as qnrA, qnrB, qnrS, oqxA, and oqxB. Three different plasmid families were detected, being most presented the ColE‐like, followed by IncFIB and IncA/C. The presence of different PMQR genes and plasmids in the isolates of the present study shows that environmental bacteria can act as reservoir of important genes of resistance to fluoroquinolones, which is of great concern, due to the potential of horizontal dissemination of these genes. Besides that, there are no studies reporting these results in Achromobacter sp. isolates.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30357960</pmid><doi>10.1111/apm.12896</doi><tpages>6</tpages></addata></record> |
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| subjects | Achromobacter Achromobacter - drug effects Achromobacter - genetics Achromobacter - growth & development Achromobacter - metabolism Achromobacter sp Amino Acid Sequence Anti-Bacterial Agents - pharmacology Antiinfectives and antibacterials Antimicrobial agents Antimicrobial resistance Bacterial Typing Techniques - methods Brazil Ciprofloxacin Ciprofloxacin - pharmacology DNA Gyrase - genetics DNA Gyrase - metabolism DNA Topoisomerase IV - genetics DNA Topoisomerase IV - metabolism Drug Resistance, Bacterial - genetics environment Fluoroquinolones Gene Expression Genes Humans Levofloxacin Levofloxacin - pharmacology Microbial Sensitivity Tests Minimum inhibitory concentration Mutation Norfloxacin Norfloxacin - pharmacology plasmid Plasmids Plasmids - chemistry Plasmids - metabolism plasmid‐mediated quinolone resistance quinolone resistance‐determining regions Quinolones Replicon Soil Microbiology Soil water Typing Water analysis Water Microbiology Water sampling |
| title | Replicon typing of plasmids in environmental Achromobacter sp. producing quinolone‐resistant determinants |
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