SPECT Imaging of Treatment-Related Tumor Necrosis Using Technetium-99m-Labeled Rhein
Purpose Noninvasive imaging of treatment-induced necrosis is important to distinguish early responders from patients resistant to the treatment plan, enabling the tailored-made therapeutic intervention. The purpose of this study was to explore the feasibility of [ 99m Tc]EDDA-HYNIC-2C-rhein for earl...
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| Veröffentlicht in: | Molecular imaging and biology 2019-08, Vol.21 (4), p.660-668 |
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| creator | Liang, Jiajia Luo, Qi Zhang, Dongjian Jin, Qiaomei Liu, Lichao Liu, Wei Gao, Meng Zhang, Jian Yin, Zhiqi |
| description | Purpose
Noninvasive imaging of treatment-induced necrosis is important to distinguish early responders from patients resistant to the treatment plan, enabling the tailored-made therapeutic intervention. The purpose of this study was to explore the feasibility of [
99m
Tc]EDDA-HYNIC-2C-rhein for early assessment of tumor response to treatment.
Procedures
In vitro
necrosis avidity of [
99m
Tc]EDDA-HYNIC-2C-rhein was evaluated in human lung cancer A549 cells treated with hyperthermia. Single photon emission–computed tomography/X-ray-computed tomography (SPECT/CT) imaging was performed in rats bearing subcutaneous W256 tumor treated with combretastatin A-4 disodium phosphate (CA4P) and rats bearing orthotopic liver W256 tumor treated with a single microwave ablation. All rats were euthanized immediately after the imaging session for biodistribution and histology studies. The mechanism of necrosis avidity for the tracer was further explored by
in vivo
blocking experiment and
in vitro
histochemistry and fluorescence staining.
Results
The uptake of [
99m
Tc]EDDA-HYNIC-2C-rhein in necrotic cells was significantly higher than that in viable cells (
p
|
| doi_str_mv | 10.1007/s11307-018-1285-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2123724118</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2254570515</sourcerecordid><originalsourceid>FETCH-LOGICAL-c400t-e305fdadc80f64a843353493ddba13fc015996759f9fcc62fde41fac868dd86f3</originalsourceid><addsrcrecordid>eNp9kU9v1DAQxS0EoqXwAbigSFy4GGbs2LGPaFWg0qqt2vRsee3xNlX-lDg58O3xagtISHDySP7Nm5n3GHuL8BEBmk8ZUULDAQ1HYRS3z9gpGg1cAIjnpVZSc9RSnLBXOT8AYINCvmQnEqQ0tRSnrL29Pt-01cXg9924r6ZUtTP5ZaBx4TfU-4Vi1a7DNFeXFOYpd7m6yweypXA_0tKtA7d24Fu_o76wN_fUja_Zi-T7TG-e3jN29-W83Xzj26uvF5vPWx5qgIWTBJWij8FA0rUvC0klaytj3HmUKQAqa3WjbLIpBC1SpBqTD0abGI1O8ox9OOo-ztP3lfLihi4H6ns_0rRmJ8q1jagRTUHf_4U-TOs8lu2cEKpWDaji1v8oFKKYrpUsFB6pgyF5puQe527w8w-H4A7BuGMwrgTjDsE4W3rePSmvu4Hi745fSRRAHIFcvsY9zX9G_1v1JwbWla8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2122100653</pqid></control><display><type>article</type><title>SPECT Imaging of Treatment-Related Tumor Necrosis Using Technetium-99m-Labeled Rhein</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Liang, Jiajia ; Luo, Qi ; Zhang, Dongjian ; Jin, Qiaomei ; Liu, Lichao ; Liu, Wei ; Gao, Meng ; Zhang, Jian ; Yin, Zhiqi</creator><creatorcontrib>Liang, Jiajia ; Luo, Qi ; Zhang, Dongjian ; Jin, Qiaomei ; Liu, Lichao ; Liu, Wei ; Gao, Meng ; Zhang, Jian ; Yin, Zhiqi</creatorcontrib><description>Purpose
Noninvasive imaging of treatment-induced necrosis is important to distinguish early responders from patients resistant to the treatment plan, enabling the tailored-made therapeutic intervention. The purpose of this study was to explore the feasibility of [
99m
Tc]EDDA-HYNIC-2C-rhein for early assessment of tumor response to treatment.
Procedures
In vitro
necrosis avidity of [
99m
Tc]EDDA-HYNIC-2C-rhein was evaluated in human lung cancer A549 cells treated with hyperthermia. Single photon emission–computed tomography/X-ray-computed tomography (SPECT/CT) imaging was performed in rats bearing subcutaneous W256 tumor treated with combretastatin A-4 disodium phosphate (CA4P) and rats bearing orthotopic liver W256 tumor treated with a single microwave ablation. All rats were euthanized immediately after the imaging session for biodistribution and histology studies. The mechanism of necrosis avidity for the tracer was further explored by
in vivo
blocking experiment and
in vitro
histochemistry and fluorescence staining.
Results
The uptake of [
99m
Tc]EDDA-HYNIC-2C-rhein in necrotic cells was significantly higher than that in viable cells (
p
< 0.05). SPECT/CT imaging showed that an obvious “hot spot” was observed in the CA4P-treated tumor while not in the control tumor at 5 h after tracer injection.
Ex vivo
γ-counting revealed that the uptake of [
99m
Tc]EDDA-HYNIC-2C-rhein in tumor was increased 3.5-fold in rats treated with CA4P compared with rats treated with vehicle. Autoradiography and corresponding H&E staining suggested that the higher overall radiotracer uptake in the treated tumors was attributed to the increased necrosis. Blocking with unlabeled HYNIC-2C-rhein demonstrated the specific binding of the radiotracer to necrotic tissues. The perfect match of autoradiograph and histochemistry staining and PI fluorescence staining revealed that necrosis avidity of the tracer may be attributable to intercalation with exposed DNA in necrotic tissues.
Conclusion
[
99m
Tc]EDDA-HYNIC-2C-rhein can image necrosis induced by anticancer therapy and holds potential for early assessment of treatment response.</description><identifier>ISSN: 1536-1632</identifier><identifier>EISSN: 1860-2002</identifier><identifier>DOI: 10.1007/s11307-018-1285-9</identifier><identifier>PMID: 30338432</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>A549 Cells ; Ablation ; Animals ; Anthraquinones - chemistry ; Autoradiography ; Avidity ; Bearing ; Computation ; Computed tomography ; Deoxyribonucleic acid ; DNA ; Feasibility studies ; Fluorescence ; Gangrene ; Histochemistry ; Histology ; Humans ; Hyperthermia ; Imaging ; Liver ; Lung cancer ; Lung Neoplasms - diagnostic imaging ; Lung Neoplasms - pathology ; Medical imaging ; Medicine ; Medicine & Public Health ; Necrosis ; Photon emission ; Radioactive tracers ; Radiology ; Rats ; Rats, Sprague-Dawley ; Research Article ; Single photon emission computed tomography ; Sodium hydrogen phosphate ; Sodium phosphate ; Staining ; Technetium ; Technetium - chemistry ; Technetium isotopes ; Tissue Distribution ; Tissues ; Tomography ; Tomography, Emission-Computed, Single-Photon ; Tomography, X-Ray Computed ; Tumors</subject><ispartof>Molecular imaging and biology, 2019-08, Vol.21 (4), p.660-668</ispartof><rights>World Molecular Imaging Society 2018</rights><rights>Molecular Imaging and Biology is a copyright of Springer, (2018). All Rights Reserved.</rights><rights>Copyright Springer Nature B.V. 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-e305fdadc80f64a843353493ddba13fc015996759f9fcc62fde41fac868dd86f3</citedby><cites>FETCH-LOGICAL-c400t-e305fdadc80f64a843353493ddba13fc015996759f9fcc62fde41fac868dd86f3</cites><orcidid>0000-0002-8402-9753</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11307-018-1285-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11307-018-1285-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30338432$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Jiajia</creatorcontrib><creatorcontrib>Luo, Qi</creatorcontrib><creatorcontrib>Zhang, Dongjian</creatorcontrib><creatorcontrib>Jin, Qiaomei</creatorcontrib><creatorcontrib>Liu, Lichao</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Gao, Meng</creatorcontrib><creatorcontrib>Zhang, Jian</creatorcontrib><creatorcontrib>Yin, Zhiqi</creatorcontrib><title>SPECT Imaging of Treatment-Related Tumor Necrosis Using Technetium-99m-Labeled Rhein</title><title>Molecular imaging and biology</title><addtitle>Mol Imaging Biol</addtitle><addtitle>Mol Imaging Biol</addtitle><description>Purpose
Noninvasive imaging of treatment-induced necrosis is important to distinguish early responders from patients resistant to the treatment plan, enabling the tailored-made therapeutic intervention. The purpose of this study was to explore the feasibility of [
99m
Tc]EDDA-HYNIC-2C-rhein for early assessment of tumor response to treatment.
Procedures
In vitro
necrosis avidity of [
99m
Tc]EDDA-HYNIC-2C-rhein was evaluated in human lung cancer A549 cells treated with hyperthermia. Single photon emission–computed tomography/X-ray-computed tomography (SPECT/CT) imaging was performed in rats bearing subcutaneous W256 tumor treated with combretastatin A-4 disodium phosphate (CA4P) and rats bearing orthotopic liver W256 tumor treated with a single microwave ablation. All rats were euthanized immediately after the imaging session for biodistribution and histology studies. The mechanism of necrosis avidity for the tracer was further explored by
in vivo
blocking experiment and
in vitro
histochemistry and fluorescence staining.
Results
The uptake of [
99m
Tc]EDDA-HYNIC-2C-rhein in necrotic cells was significantly higher than that in viable cells (
p
< 0.05). SPECT/CT imaging showed that an obvious “hot spot” was observed in the CA4P-treated tumor while not in the control tumor at 5 h after tracer injection.
Ex vivo
γ-counting revealed that the uptake of [
99m
Tc]EDDA-HYNIC-2C-rhein in tumor was increased 3.5-fold in rats treated with CA4P compared with rats treated with vehicle. Autoradiography and corresponding H&E staining suggested that the higher overall radiotracer uptake in the treated tumors was attributed to the increased necrosis. Blocking with unlabeled HYNIC-2C-rhein demonstrated the specific binding of the radiotracer to necrotic tissues. The perfect match of autoradiograph and histochemistry staining and PI fluorescence staining revealed that necrosis avidity of the tracer may be attributable to intercalation with exposed DNA in necrotic tissues.
Conclusion
[
99m
Tc]EDDA-HYNIC-2C-rhein can image necrosis induced by anticancer therapy and holds potential for early assessment of treatment response.</description><subject>A549 Cells</subject><subject>Ablation</subject><subject>Animals</subject><subject>Anthraquinones - chemistry</subject><subject>Autoradiography</subject><subject>Avidity</subject><subject>Bearing</subject><subject>Computation</subject><subject>Computed tomography</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Feasibility studies</subject><subject>Fluorescence</subject><subject>Gangrene</subject><subject>Histochemistry</subject><subject>Histology</subject><subject>Humans</subject><subject>Hyperthermia</subject><subject>Imaging</subject><subject>Liver</subject><subject>Lung cancer</subject><subject>Lung Neoplasms - diagnostic imaging</subject><subject>Lung Neoplasms - pathology</subject><subject>Medical imaging</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Necrosis</subject><subject>Photon emission</subject><subject>Radioactive tracers</subject><subject>Radiology</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Research Article</subject><subject>Single photon emission computed tomography</subject><subject>Sodium hydrogen phosphate</subject><subject>Sodium phosphate</subject><subject>Staining</subject><subject>Technetium</subject><subject>Technetium - chemistry</subject><subject>Technetium isotopes</subject><subject>Tissue Distribution</subject><subject>Tissues</subject><subject>Tomography</subject><subject>Tomography, Emission-Computed, Single-Photon</subject><subject>Tomography, X-Ray Computed</subject><subject>Tumors</subject><issn>1536-1632</issn><issn>1860-2002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kU9v1DAQxS0EoqXwAbigSFy4GGbs2LGPaFWg0qqt2vRsee3xNlX-lDg58O3xagtISHDySP7Nm5n3GHuL8BEBmk8ZUULDAQ1HYRS3z9gpGg1cAIjnpVZSc9RSnLBXOT8AYINCvmQnEqQ0tRSnrL29Pt-01cXg9924r6ZUtTP5ZaBx4TfU-4Vi1a7DNFeXFOYpd7m6yweypXA_0tKtA7d24Fu_o76wN_fUja_Zi-T7TG-e3jN29-W83Xzj26uvF5vPWx5qgIWTBJWij8FA0rUvC0klaytj3HmUKQAqa3WjbLIpBC1SpBqTD0abGI1O8ox9OOo-ztP3lfLihi4H6ns_0rRmJ8q1jagRTUHf_4U-TOs8lu2cEKpWDaji1v8oFKKYrpUsFB6pgyF5puQe527w8w-H4A7BuGMwrgTjDsE4W3rePSmvu4Hi745fSRRAHIFcvsY9zX9G_1v1JwbWla8</recordid><startdate>20190801</startdate><enddate>20190801</enddate><creator>Liang, Jiajia</creator><creator>Luo, Qi</creator><creator>Zhang, Dongjian</creator><creator>Jin, Qiaomei</creator><creator>Liu, Lichao</creator><creator>Liu, Wei</creator><creator>Gao, Meng</creator><creator>Zhang, Jian</creator><creator>Yin, Zhiqi</creator><general>Springer International Publishing</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>L6V</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8402-9753</orcidid></search><sort><creationdate>20190801</creationdate><title>SPECT Imaging of Treatment-Related Tumor Necrosis Using Technetium-99m-Labeled Rhein</title><author>Liang, Jiajia ; Luo, Qi ; Zhang, Dongjian ; Jin, Qiaomei ; Liu, Lichao ; Liu, Wei ; Gao, Meng ; Zhang, Jian ; Yin, Zhiqi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c400t-e305fdadc80f64a843353493ddba13fc015996759f9fcc62fde41fac868dd86f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>A549 Cells</topic><topic>Ablation</topic><topic>Animals</topic><topic>Anthraquinones - chemistry</topic><topic>Autoradiography</topic><topic>Avidity</topic><topic>Bearing</topic><topic>Computation</topic><topic>Computed tomography</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Feasibility studies</topic><topic>Fluorescence</topic><topic>Gangrene</topic><topic>Histochemistry</topic><topic>Histology</topic><topic>Humans</topic><topic>Hyperthermia</topic><topic>Imaging</topic><topic>Liver</topic><topic>Lung cancer</topic><topic>Lung Neoplasms - diagnostic imaging</topic><topic>Lung Neoplasms - pathology</topic><topic>Medical imaging</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Necrosis</topic><topic>Photon emission</topic><topic>Radioactive tracers</topic><topic>Radiology</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Research Article</topic><topic>Single photon emission computed tomography</topic><topic>Sodium hydrogen phosphate</topic><topic>Sodium phosphate</topic><topic>Staining</topic><topic>Technetium</topic><topic>Technetium - chemistry</topic><topic>Technetium isotopes</topic><topic>Tissue Distribution</topic><topic>Tissues</topic><topic>Tomography</topic><topic>Tomography, Emission-Computed, Single-Photon</topic><topic>Tomography, X-Ray Computed</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Liang, Jiajia</creatorcontrib><creatorcontrib>Luo, Qi</creatorcontrib><creatorcontrib>Zhang, Dongjian</creatorcontrib><creatorcontrib>Jin, Qiaomei</creatorcontrib><creatorcontrib>Liu, Lichao</creatorcontrib><creatorcontrib>Liu, Wei</creatorcontrib><creatorcontrib>Gao, Meng</creatorcontrib><creatorcontrib>Zhang, Jian</creatorcontrib><creatorcontrib>Yin, Zhiqi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular imaging and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Jiajia</au><au>Luo, Qi</au><au>Zhang, Dongjian</au><au>Jin, Qiaomei</au><au>Liu, Lichao</au><au>Liu, Wei</au><au>Gao, Meng</au><au>Zhang, Jian</au><au>Yin, Zhiqi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>SPECT Imaging of Treatment-Related Tumor Necrosis Using Technetium-99m-Labeled Rhein</atitle><jtitle>Molecular imaging and biology</jtitle><stitle>Mol Imaging Biol</stitle><addtitle>Mol Imaging Biol</addtitle><date>2019-08-01</date><risdate>2019</risdate><volume>21</volume><issue>4</issue><spage>660</spage><epage>668</epage><pages>660-668</pages><issn>1536-1632</issn><eissn>1860-2002</eissn><abstract>Purpose
Noninvasive imaging of treatment-induced necrosis is important to distinguish early responders from patients resistant to the treatment plan, enabling the tailored-made therapeutic intervention. The purpose of this study was to explore the feasibility of [
99m
Tc]EDDA-HYNIC-2C-rhein for early assessment of tumor response to treatment.
Procedures
In vitro
necrosis avidity of [
99m
Tc]EDDA-HYNIC-2C-rhein was evaluated in human lung cancer A549 cells treated with hyperthermia. Single photon emission–computed tomography/X-ray-computed tomography (SPECT/CT) imaging was performed in rats bearing subcutaneous W256 tumor treated with combretastatin A-4 disodium phosphate (CA4P) and rats bearing orthotopic liver W256 tumor treated with a single microwave ablation. All rats were euthanized immediately after the imaging session for biodistribution and histology studies. The mechanism of necrosis avidity for the tracer was further explored by
in vivo
blocking experiment and
in vitro
histochemistry and fluorescence staining.
Results
The uptake of [
99m
Tc]EDDA-HYNIC-2C-rhein in necrotic cells was significantly higher than that in viable cells (
p
< 0.05). SPECT/CT imaging showed that an obvious “hot spot” was observed in the CA4P-treated tumor while not in the control tumor at 5 h after tracer injection.
Ex vivo
γ-counting revealed that the uptake of [
99m
Tc]EDDA-HYNIC-2C-rhein in tumor was increased 3.5-fold in rats treated with CA4P compared with rats treated with vehicle. Autoradiography and corresponding H&E staining suggested that the higher overall radiotracer uptake in the treated tumors was attributed to the increased necrosis. Blocking with unlabeled HYNIC-2C-rhein demonstrated the specific binding of the radiotracer to necrotic tissues. The perfect match of autoradiograph and histochemistry staining and PI fluorescence staining revealed that necrosis avidity of the tracer may be attributable to intercalation with exposed DNA in necrotic tissues.
Conclusion
[
99m
Tc]EDDA-HYNIC-2C-rhein can image necrosis induced by anticancer therapy and holds potential for early assessment of treatment response.</abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>30338432</pmid><doi>10.1007/s11307-018-1285-9</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-8402-9753</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Molecular imaging and biology, 2019-08, Vol.21 (4), p.660-668 |
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| language | eng |
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| source | MEDLINE; SpringerLink Journals |
| subjects | A549 Cells Ablation Animals Anthraquinones - chemistry Autoradiography Avidity Bearing Computation Computed tomography Deoxyribonucleic acid DNA Feasibility studies Fluorescence Gangrene Histochemistry Histology Humans Hyperthermia Imaging Liver Lung cancer Lung Neoplasms - diagnostic imaging Lung Neoplasms - pathology Medical imaging Medicine Medicine & Public Health Necrosis Photon emission Radioactive tracers Radiology Rats Rats, Sprague-Dawley Research Article Single photon emission computed tomography Sodium hydrogen phosphate Sodium phosphate Staining Technetium Technetium - chemistry Technetium isotopes Tissue Distribution Tissues Tomography Tomography, Emission-Computed, Single-Photon Tomography, X-Ray Computed Tumors |
| title | SPECT Imaging of Treatment-Related Tumor Necrosis Using Technetium-99m-Labeled Rhein |
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