Long noncoding RNA HOTAIR silencing inhibits invasion and proliferation of human colon cancer LoVo cells via regulating IGF2BP2
Colon cancer is one of the most life‐threatening malignancies worldwide. Long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) is a cancer‐associated biomarker involved in the metastasis and prognosis of several cancers. However, whether and how HOTAIR affects colon cancer progression is...
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| Veröffentlicht in: | Journal of cellular biochemistry 2019-02, Vol.120 (2), p.1221-1231 |
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| creator | Wu, Xue‐Liang Lu, Rui‐Yun Wang, Li‐Kun Wang, Yuan‐Yuan Dai, Yong‐Jun Wang, Chen‐Yu Yang, Yong‐Jiang Guo, Fei Xue, Jun Yang, Dong‐Dong |
| description | Colon cancer is one of the most life‐threatening malignancies worldwide. Long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) is a cancer‐associated biomarker involved in the metastasis and prognosis of several cancers. However, whether and how HOTAIR affects colon cancer progression is still unclear. Consequently, we used RNA interference to knock down HOTAIR to explore its effects on human colon cancer cells. The dual luciferase reporter gene assay was initially used for testify the regulating relationship between lncRNA HOTAIR and insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2). We determined the expressions of HOTAIR, IGF2BP2, E‐cadherin, and vimentin. Meanwhile, cell growth, cycle and apoptosis, migration, and invasion were assayed. LoVo cells were transplanted into nude mice, and tumor formation and microvessel density were evaluated. LncRNA HOTAIR positively regulated IGF2BP2. Besides, the expressions of HOTAIR and E‐cadherin and the apoptosis were increased, while the expressions of IGF2BP2 and vimentin, the growth, invasion and migration of LoVo cells, the average tumor weight, and microvessel density value were decreased. Of importance, overexpressed IGF2BP2 could reverse the above impacts. Taken together, the current study indicates that silencing of HOTAIR could inhibit the invasion, proliferation, and migration, and promote apoptosis of colon cancer LoVo cells through suppressing IGF2BP2 and the epithelial‐mesenchymal transition.
Silencing of HOX transcript antisense RNA (HOTAIR) could inhibit the invasion, proliferation, and migration, and promote apoptosis of colon cancer LoVo cells through suppressing insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) and the epithelial‐mesenchymal transition. |
| doi_str_mv | 10.1002/jcb.27079 |
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Silencing of HOX transcript antisense RNA (HOTAIR) could inhibit the invasion, proliferation, and migration, and promote apoptosis of colon cancer LoVo cells through suppressing insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) and the epithelial‐mesenchymal transition.</description><identifier>ISSN: 0730-2312</identifier><identifier>EISSN: 1097-4644</identifier><identifier>DOI: 10.1002/jcb.27079</identifier><identifier>PMID: 30335892</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Antisense RNA ; Apoptosis ; Biomarkers ; Cancer ; Cell migration ; Cell proliferation ; Colon ; Colon cancer ; Colorectal cancer ; Density ; E-cadherin ; epithelial‐mesenchymal transition (EMT) ; Growth factors ; human colon cancer lovo cell ; Insulin ; insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) ; invasion ; long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) ; Mesenchyme ; Metastases ; proliferation ; Proteins ; Reporter gene ; Ribonucleic acid ; RNA ; RNA-mediated interference ; Transcription ; Tumors ; Vimentin ; Weight</subject><ispartof>Journal of cellular biochemistry, 2019-02, Vol.120 (2), p.1221-1231</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3539-b395fd160d81420c1cd0cfdc89f1e37935769318b6705774b8d938ae6c903fd93</citedby><cites>FETCH-LOGICAL-c3539-b395fd160d81420c1cd0cfdc89f1e37935769318b6705774b8d938ae6c903fd93</cites><orcidid>0000-0002-3340-0145</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcb.27079$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcb.27079$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30335892$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wu, Xue‐Liang</creatorcontrib><creatorcontrib>Lu, Rui‐Yun</creatorcontrib><creatorcontrib>Wang, Li‐Kun</creatorcontrib><creatorcontrib>Wang, Yuan‐Yuan</creatorcontrib><creatorcontrib>Dai, Yong‐Jun</creatorcontrib><creatorcontrib>Wang, Chen‐Yu</creatorcontrib><creatorcontrib>Yang, Yong‐Jiang</creatorcontrib><creatorcontrib>Guo, Fei</creatorcontrib><creatorcontrib>Xue, Jun</creatorcontrib><creatorcontrib>Yang, Dong‐Dong</creatorcontrib><title>Long noncoding RNA HOTAIR silencing inhibits invasion and proliferation of human colon cancer LoVo cells via regulating IGF2BP2</title><title>Journal of cellular biochemistry</title><addtitle>J Cell Biochem</addtitle><description>Colon cancer is one of the most life‐threatening malignancies worldwide. Long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) is a cancer‐associated biomarker involved in the metastasis and prognosis of several cancers. However, whether and how HOTAIR affects colon cancer progression is still unclear. Consequently, we used RNA interference to knock down HOTAIR to explore its effects on human colon cancer cells. The dual luciferase reporter gene assay was initially used for testify the regulating relationship between lncRNA HOTAIR and insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2). We determined the expressions of HOTAIR, IGF2BP2, E‐cadherin, and vimentin. Meanwhile, cell growth, cycle and apoptosis, migration, and invasion were assayed. LoVo cells were transplanted into nude mice, and tumor formation and microvessel density were evaluated. LncRNA HOTAIR positively regulated IGF2BP2. Besides, the expressions of HOTAIR and E‐cadherin and the apoptosis were increased, while the expressions of IGF2BP2 and vimentin, the growth, invasion and migration of LoVo cells, the average tumor weight, and microvessel density value were decreased. Of importance, overexpressed IGF2BP2 could reverse the above impacts. Taken together, the current study indicates that silencing of HOTAIR could inhibit the invasion, proliferation, and migration, and promote apoptosis of colon cancer LoVo cells through suppressing IGF2BP2 and the epithelial‐mesenchymal transition.
Silencing of HOX transcript antisense RNA (HOTAIR) could inhibit the invasion, proliferation, and migration, and promote apoptosis of colon cancer LoVo cells through suppressing insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) and the epithelial‐mesenchymal transition.</description><subject>Antisense RNA</subject><subject>Apoptosis</subject><subject>Biomarkers</subject><subject>Cancer</subject><subject>Cell migration</subject><subject>Cell proliferation</subject><subject>Colon</subject><subject>Colon cancer</subject><subject>Colorectal cancer</subject><subject>Density</subject><subject>E-cadherin</subject><subject>epithelial‐mesenchymal transition (EMT)</subject><subject>Growth factors</subject><subject>human colon cancer lovo cell</subject><subject>Insulin</subject><subject>insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2)</subject><subject>invasion</subject><subject>long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR)</subject><subject>Mesenchyme</subject><subject>Metastases</subject><subject>proliferation</subject><subject>Proteins</subject><subject>Reporter gene</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>RNA-mediated interference</subject><subject>Transcription</subject><subject>Tumors</subject><subject>Vimentin</subject><subject>Weight</subject><issn>0730-2312</issn><issn>1097-4644</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kcFOGzEQhq2qCALl0BeoLPXSHjYZe3bX62OICgRFpUKB68rr9QZHGxvsLBUnXr1OAz0g9eTfo0-fRvMT8pnBmAHwyVo3Yy5AyA9kxECKLC_z_CMZgUDIODJ-RI5jXAOAlMgPyRECYlFJPiIvC-9W1HmnfWtTuvk5pZfXy-n8hkbbG6d3Q-vubWO3MYUnFa13VLmWPgTf284Etd1NfEfvh41yVPs-fbVy2gS68HeeatP3kT5ZRYNZDX3ik3N-cc7PfvFP5KBTfTSnr-8JuT3_sZxdZovri_lsusg0FiizBmXRtayEtmI5B810C7prdSU7ZlBILEQpkVVNKaAQIm-qVmKlTKklYJfyCfm296atHwcTt_XGxt1iyhk_xJozjoJhEiX06zt07Yfg0naJKopKVGWOifq-p3TwMQbT1Q_BblR4rhnUu1bq1Er9t5XEfnk1Ds3GtP_ItxoSMNkDv9PNn_9vqq9mZ3vlH0JClK4</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Wu, Xue‐Liang</creator><creator>Lu, Rui‐Yun</creator><creator>Wang, Li‐Kun</creator><creator>Wang, Yuan‐Yuan</creator><creator>Dai, Yong‐Jun</creator><creator>Wang, Chen‐Yu</creator><creator>Yang, Yong‐Jiang</creator><creator>Guo, Fei</creator><creator>Xue, Jun</creator><creator>Yang, Dong‐Dong</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7T7</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-3340-0145</orcidid></search><sort><creationdate>201902</creationdate><title>Long noncoding RNA HOTAIR silencing inhibits invasion and proliferation of human colon cancer LoVo cells via regulating IGF2BP2</title><author>Wu, Xue‐Liang ; Lu, Rui‐Yun ; Wang, Li‐Kun ; Wang, Yuan‐Yuan ; Dai, Yong‐Jun ; Wang, Chen‐Yu ; Yang, Yong‐Jiang ; Guo, Fei ; Xue, Jun ; Yang, Dong‐Dong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3539-b395fd160d81420c1cd0cfdc89f1e37935769318b6705774b8d938ae6c903fd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antisense RNA</topic><topic>Apoptosis</topic><topic>Biomarkers</topic><topic>Cancer</topic><topic>Cell migration</topic><topic>Cell proliferation</topic><topic>Colon</topic><topic>Colon cancer</topic><topic>Colorectal cancer</topic><topic>Density</topic><topic>E-cadherin</topic><topic>epithelial‐mesenchymal transition (EMT)</topic><topic>Growth factors</topic><topic>human colon cancer lovo cell</topic><topic>Insulin</topic><topic>insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2)</topic><topic>invasion</topic><topic>long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR)</topic><topic>Mesenchyme</topic><topic>Metastases</topic><topic>proliferation</topic><topic>Proteins</topic><topic>Reporter gene</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>RNA-mediated interference</topic><topic>Transcription</topic><topic>Tumors</topic><topic>Vimentin</topic><topic>Weight</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wu, Xue‐Liang</creatorcontrib><creatorcontrib>Lu, Rui‐Yun</creatorcontrib><creatorcontrib>Wang, Li‐Kun</creatorcontrib><creatorcontrib>Wang, Yuan‐Yuan</creatorcontrib><creatorcontrib>Dai, Yong‐Jun</creatorcontrib><creatorcontrib>Wang, Chen‐Yu</creatorcontrib><creatorcontrib>Yang, Yong‐Jiang</creatorcontrib><creatorcontrib>Guo, Fei</creatorcontrib><creatorcontrib>Xue, Jun</creatorcontrib><creatorcontrib>Yang, Dong‐Dong</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wu, Xue‐Liang</au><au>Lu, Rui‐Yun</au><au>Wang, Li‐Kun</au><au>Wang, Yuan‐Yuan</au><au>Dai, Yong‐Jun</au><au>Wang, Chen‐Yu</au><au>Yang, Yong‐Jiang</au><au>Guo, Fei</au><au>Xue, Jun</au><au>Yang, Dong‐Dong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Long noncoding RNA HOTAIR silencing inhibits invasion and proliferation of human colon cancer LoVo cells via regulating IGF2BP2</atitle><jtitle>Journal of cellular biochemistry</jtitle><addtitle>J Cell Biochem</addtitle><date>2019-02</date><risdate>2019</risdate><volume>120</volume><issue>2</issue><spage>1221</spage><epage>1231</epage><pages>1221-1231</pages><issn>0730-2312</issn><eissn>1097-4644</eissn><abstract>Colon cancer is one of the most life‐threatening malignancies worldwide. Long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) is a cancer‐associated biomarker involved in the metastasis and prognosis of several cancers. However, whether and how HOTAIR affects colon cancer progression is still unclear. Consequently, we used RNA interference to knock down HOTAIR to explore its effects on human colon cancer cells. The dual luciferase reporter gene assay was initially used for testify the regulating relationship between lncRNA HOTAIR and insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2). We determined the expressions of HOTAIR, IGF2BP2, E‐cadherin, and vimentin. Meanwhile, cell growth, cycle and apoptosis, migration, and invasion were assayed. LoVo cells were transplanted into nude mice, and tumor formation and microvessel density were evaluated. LncRNA HOTAIR positively regulated IGF2BP2. Besides, the expressions of HOTAIR and E‐cadherin and the apoptosis were increased, while the expressions of IGF2BP2 and vimentin, the growth, invasion and migration of LoVo cells, the average tumor weight, and microvessel density value were decreased. Of importance, overexpressed IGF2BP2 could reverse the above impacts. Taken together, the current study indicates that silencing of HOTAIR could inhibit the invasion, proliferation, and migration, and promote apoptosis of colon cancer LoVo cells through suppressing IGF2BP2 and the epithelial‐mesenchymal transition.
Silencing of HOX transcript antisense RNA (HOTAIR) could inhibit the invasion, proliferation, and migration, and promote apoptosis of colon cancer LoVo cells through suppressing insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) and the epithelial‐mesenchymal transition.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30335892</pmid><doi>10.1002/jcb.27079</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-3340-0145</orcidid></addata></record> |
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| subjects | Antisense RNA Apoptosis Biomarkers Cancer Cell migration Cell proliferation Colon Colon cancer Colorectal cancer Density E-cadherin epithelial‐mesenchymal transition (EMT) Growth factors human colon cancer lovo cell Insulin insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2) invasion long noncoding RNA (lncRNA) HOX transcript antisense RNA (HOTAIR) Mesenchyme Metastases proliferation Proteins Reporter gene Ribonucleic acid RNA RNA-mediated interference Transcription Tumors Vimentin Weight |
| title | Long noncoding RNA HOTAIR silencing inhibits invasion and proliferation of human colon cancer LoVo cells via regulating IGF2BP2 |
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