Characterization of Dermanyssus gallinae (Acarina: Dermanissydae) by sequence analysis of the ribosomal internal transcribed spacer regions
In the present work mites previously identified as Dermanyssus gallinae De Geer (Acari, Mesostigmata) using morphological keys were investigated by molecular tools. The complete internal transcribed spacer 1 (ITS1), 5.8S ribosomal DNA, and ITS2 region of the ribosomal DNA from mites were amplified a...
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| Veröffentlicht in: | Veterinary research communications 2009-10, Vol.33 (7), p.611-618 |
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| creator | Potenza, L Cafiero, M. A Camarda, A La Salandra, G Cucchiarini, L Dachà, M |
| description | In the present work mites previously identified as Dermanyssus gallinae De Geer (Acari, Mesostigmata) using morphological keys were investigated by molecular tools. The complete internal transcribed spacer 1 (ITS1), 5.8S ribosomal DNA, and ITS2 region of the ribosomal DNA from mites were amplified and sequenced to examine the level of sequence variations and to explore the feasibility of using this region in the identification of this mite. Conserved primers located at the 3'end of 18S and at the 5'start of 28S rRNA genes were used first, and amplified fragments were sequenced. Sequence analyses showed no variation in 5.8S and ITS2 region while slight intraspecific variations involving substitutions as well as deletions concentrated in the ITS1 region. Based on the sequence analyses a nested PCR of the ITS2 region followed by RFLP analyses has been set up in the attempt to provide a rapid molecular diagnostic tool of D. gallinae. |
| doi_str_mv | 10.1007/s11259-009-9210-y |
| format | Article |
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A ; Camarda, A ; La Salandra, G ; Cucchiarini, L ; Dachà, M</creator><creatorcontrib>Potenza, L ; Cafiero, M. A ; Camarda, A ; La Salandra, G ; Cucchiarini, L ; Dachà, M</creatorcontrib><description>In the present work mites previously identified as Dermanyssus gallinae De Geer (Acari, Mesostigmata) using morphological keys were investigated by molecular tools. The complete internal transcribed spacer 1 (ITS1), 5.8S ribosomal DNA, and ITS2 region of the ribosomal DNA from mites were amplified and sequenced to examine the level of sequence variations and to explore the feasibility of using this region in the identification of this mite. Conserved primers located at the 3'end of 18S and at the 5'start of 28S rRNA genes were used first, and amplified fragments were sequenced. Sequence analyses showed no variation in 5.8S and ITS2 region while slight intraspecific variations involving substitutions as well as deletions concentrated in the ITS1 region. Based on the sequence analyses a nested PCR of the ITS2 region followed by RFLP analyses has been set up in the attempt to provide a rapid molecular diagnostic tool of D. gallinae.</description><identifier>ISSN: 0165-7380</identifier><identifier>EISSN: 1573-7446</identifier><identifier>DOI: 10.1007/s11259-009-9210-y</identifier><identifier>PMID: 19214768</identifier><language>eng</language><publisher>Dordrecht: Dordrecht : Springer Netherlands</publisher><subject>Acari ; Animals ; Biomedical and Life Sciences ; Columbidae - parasitology ; Dermanyssus gallinae ; disease diagnosis ; DNA Primers ; DNA, Ribosomal - genetics ; DNA, Ribosomal - isolation & purification ; DNA, Ribosomal Spacer - genetics ; DNA, Ribosomal Spacer - isolation & purification ; ectoparasites ; Gene Amplification ; genetic variation ; geographical variation ; internal transcribed spacers ; Life Sciences ; Mesostigmata ; Mite Infestations - genetics ; mites ; Mites - genetics ; molecular epidemiology ; Molecular Sequence Data ; Original Article ; parasitoses ; Polymerase Chain Reaction ; Poultry - parasitology ; Poultry Diseases - parasitology ; rapid methods ; restriction fragment length polymorphism ; ribosomal DNA ; sequence analysis ; taxonomy ; Transcription, Genetic ; validity ; Veterinary Medicine/Veterinary Science ; Zoology</subject><ispartof>Veterinary research communications, 2009-10, Vol.33 (7), p.611-618</ispartof><rights>Springer Science+Business Media B.V. 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c425t-833479b5fb197436db5f9793246e2831987490b79b0e91f0efe25cebe4eaeab93</citedby><cites>FETCH-LOGICAL-c425t-833479b5fb197436db5f9793246e2831987490b79b0e91f0efe25cebe4eaeab93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11259-009-9210-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11259-009-9210-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19214768$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Potenza, L</creatorcontrib><creatorcontrib>Cafiero, M. A</creatorcontrib><creatorcontrib>Camarda, A</creatorcontrib><creatorcontrib>La Salandra, G</creatorcontrib><creatorcontrib>Cucchiarini, L</creatorcontrib><creatorcontrib>Dachà, M</creatorcontrib><title>Characterization of Dermanyssus gallinae (Acarina: Dermanissydae) by sequence analysis of the ribosomal internal transcribed spacer regions</title><title>Veterinary research communications</title><addtitle>Vet Res Commun</addtitle><addtitle>Vet Res Commun</addtitle><description>In the present work mites previously identified as Dermanyssus gallinae De Geer (Acari, Mesostigmata) using morphological keys were investigated by molecular tools. The complete internal transcribed spacer 1 (ITS1), 5.8S ribosomal DNA, and ITS2 region of the ribosomal DNA from mites were amplified and sequenced to examine the level of sequence variations and to explore the feasibility of using this region in the identification of this mite. Conserved primers located at the 3'end of 18S and at the 5'start of 28S rRNA genes were used first, and amplified fragments were sequenced. Sequence analyses showed no variation in 5.8S and ITS2 region while slight intraspecific variations involving substitutions as well as deletions concentrated in the ITS1 region. Based on the sequence analyses a nested PCR of the ITS2 region followed by RFLP analyses has been set up in the attempt to provide a rapid molecular diagnostic tool of D. gallinae.</description><subject>Acari</subject><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Columbidae - parasitology</subject><subject>Dermanyssus gallinae</subject><subject>disease diagnosis</subject><subject>DNA Primers</subject><subject>DNA, Ribosomal - genetics</subject><subject>DNA, Ribosomal - isolation & purification</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>DNA, Ribosomal Spacer - isolation & purification</subject><subject>ectoparasites</subject><subject>Gene Amplification</subject><subject>genetic variation</subject><subject>geographical variation</subject><subject>internal transcribed spacers</subject><subject>Life Sciences</subject><subject>Mesostigmata</subject><subject>Mite Infestations - genetics</subject><subject>mites</subject><subject>Mites - genetics</subject><subject>molecular epidemiology</subject><subject>Molecular Sequence Data</subject><subject>Original Article</subject><subject>parasitoses</subject><subject>Polymerase Chain Reaction</subject><subject>Poultry - parasitology</subject><subject>Poultry Diseases - parasitology</subject><subject>rapid methods</subject><subject>restriction fragment length polymorphism</subject><subject>ribosomal DNA</subject><subject>sequence analysis</subject><subject>taxonomy</subject><subject>Transcription, Genetic</subject><subject>validity</subject><subject>Veterinary Medicine/Veterinary Science</subject><subject>Zoology</subject><issn>0165-7380</issn><issn>1573-7446</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp9kctuFDEQRS0EIpPAB7ABiwWCRYPLdj-cXTThJUViAVlb1T3VE0f9GFzdi-YX-Gk89EiRWLCypXvq3rKvEC9AvQelyg8MoHOXKeUyp0FlyyOxgbw0WWlt8VhsFBR5VppKnYlz5nuVwEqZp-IMEm7LotqI39s7jNhMFMMvnMI4yLGV1xR7HBbmmeUeuy4MSPLtVYMx3S5PcmBedkjvZL1Ipp8zDQ1JHLBbOPDRZbojGUM98thjJ8OQMpIqp4gDN0mgneQDNhRlpH1K5mfiSYsd0_PTeSFuP338sf2S3Xz7_HV7dZM1VudTVhljS1fnbQ2utKbYpasrndG2IF0ZcFVpnaoToshBq6glnTdUkyUkrJ25EG9W30Mc0948-T5wQ12HA40zew0aqsrYBL7-B7wf5-MjEpMytAKjEgQr1MSROVLrDzH0GBcPyh9r8mtNPv2-P9bklzTz8mQ81z3tHiZOvSRArwAnadhTfEj-n-urdajF0eM-Bva33_8uCUUFYJX5A8mLqWs</recordid><startdate>20091001</startdate><enddate>20091001</enddate><creator>Potenza, L</creator><creator>Cafiero, M. A</creator><creator>Camarda, A</creator><creator>La Salandra, G</creator><creator>Cucchiarini, L</creator><creator>Dachà, M</creator><general>Dordrecht : Springer Netherlands</general><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7SS</scope><scope>7TM</scope></search><sort><creationdate>20091001</creationdate><title>Characterization of Dermanyssus gallinae (Acarina: Dermanissydae) by sequence analysis of the ribosomal internal transcribed spacer regions</title><author>Potenza, L ; Cafiero, M. A ; Camarda, A ; La Salandra, G ; Cucchiarini, L ; Dachà, M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c425t-833479b5fb197436db5f9793246e2831987490b79b0e91f0efe25cebe4eaeab93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Acari</topic><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Columbidae - parasitology</topic><topic>Dermanyssus gallinae</topic><topic>disease diagnosis</topic><topic>DNA Primers</topic><topic>DNA, Ribosomal - genetics</topic><topic>DNA, Ribosomal - isolation & purification</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>DNA, Ribosomal Spacer - isolation & purification</topic><topic>ectoparasites</topic><topic>Gene Amplification</topic><topic>genetic variation</topic><topic>geographical variation</topic><topic>internal transcribed spacers</topic><topic>Life Sciences</topic><topic>Mesostigmata</topic><topic>Mite Infestations - genetics</topic><topic>mites</topic><topic>Mites - genetics</topic><topic>molecular epidemiology</topic><topic>Molecular Sequence Data</topic><topic>Original Article</topic><topic>parasitoses</topic><topic>Polymerase Chain Reaction</topic><topic>Poultry - parasitology</topic><topic>Poultry Diseases - parasitology</topic><topic>rapid methods</topic><topic>restriction fragment length polymorphism</topic><topic>ribosomal DNA</topic><topic>sequence analysis</topic><topic>taxonomy</topic><topic>Transcription, Genetic</topic><topic>validity</topic><topic>Veterinary Medicine/Veterinary Science</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Potenza, L</creatorcontrib><creatorcontrib>Cafiero, M. A</creatorcontrib><creatorcontrib>Camarda, A</creatorcontrib><creatorcontrib>La Salandra, G</creatorcontrib><creatorcontrib>Cucchiarini, L</creatorcontrib><creatorcontrib>Dachà, M</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection (ProQuest)</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><jtitle>Veterinary research communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Potenza, L</au><au>Cafiero, M. A</au><au>Camarda, A</au><au>La Salandra, G</au><au>Cucchiarini, L</au><au>Dachà, M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of Dermanyssus gallinae (Acarina: Dermanissydae) by sequence analysis of the ribosomal internal transcribed spacer regions</atitle><jtitle>Veterinary research communications</jtitle><stitle>Vet Res Commun</stitle><addtitle>Vet Res Commun</addtitle><date>2009-10-01</date><risdate>2009</risdate><volume>33</volume><issue>7</issue><spage>611</spage><epage>618</epage><pages>611-618</pages><issn>0165-7380</issn><eissn>1573-7446</eissn><abstract>In the present work mites previously identified as Dermanyssus gallinae De Geer (Acari, Mesostigmata) using morphological keys were investigated by molecular tools. The complete internal transcribed spacer 1 (ITS1), 5.8S ribosomal DNA, and ITS2 region of the ribosomal DNA from mites were amplified and sequenced to examine the level of sequence variations and to explore the feasibility of using this region in the identification of this mite. Conserved primers located at the 3'end of 18S and at the 5'start of 28S rRNA genes were used first, and amplified fragments were sequenced. Sequence analyses showed no variation in 5.8S and ITS2 region while slight intraspecific variations involving substitutions as well as deletions concentrated in the ITS1 region. Based on the sequence analyses a nested PCR of the ITS2 region followed by RFLP analyses has been set up in the attempt to provide a rapid molecular diagnostic tool of D. gallinae.</abstract><cop>Dordrecht</cop><pub>Dordrecht : Springer Netherlands</pub><pmid>19214768</pmid><doi>10.1007/s11259-009-9210-y</doi><tpages>8</tpages></addata></record> |
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| subjects | Acari Animals Biomedical and Life Sciences Columbidae - parasitology Dermanyssus gallinae disease diagnosis DNA Primers DNA, Ribosomal - genetics DNA, Ribosomal - isolation & purification DNA, Ribosomal Spacer - genetics DNA, Ribosomal Spacer - isolation & purification ectoparasites Gene Amplification genetic variation geographical variation internal transcribed spacers Life Sciences Mesostigmata Mite Infestations - genetics mites Mites - genetics molecular epidemiology Molecular Sequence Data Original Article parasitoses Polymerase Chain Reaction Poultry - parasitology Poultry Diseases - parasitology rapid methods restriction fragment length polymorphism ribosomal DNA sequence analysis taxonomy Transcription, Genetic validity Veterinary Medicine/Veterinary Science Zoology |
| title | Characterization of Dermanyssus gallinae (Acarina: Dermanissydae) by sequence analysis of the ribosomal internal transcribed spacer regions |
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