Highly luminescent green-emitting Au nanocluster-based multiplex lateral flow immunoassay for ultrasensitive detection of clenbuterol and ractopamine

A multiplex lateral flow immunoassay sensor based on highly luminescent green-emitting Au nanoclusters (AuNCs-MLFIA sensor) was successfully established for the simultaneous and quantitative determination of clenbuterol (Clen) and ractopamine (RAC) in swine urine. The antigens of Clen and RAC were d...

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Veröffentlicht in:Analytica chimica acta 2018-12, Vol.1040, p.143-149
Hauptverfasser: Peng, Tao, Wang, Jianyi, Zhao, Sijun, Zeng, Yuyang, Zheng, Pimiao, Liang, Demei, Mari, Ghulam Mujtaba, Jiang, Haiyang
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container_start_page 143
container_title Analytica chimica acta
container_volume 1040
creator Peng, Tao
Wang, Jianyi
Zhao, Sijun
Zeng, Yuyang
Zheng, Pimiao
Liang, Demei
Mari, Ghulam Mujtaba
Jiang, Haiyang
description A multiplex lateral flow immunoassay sensor based on highly luminescent green-emitting Au nanoclusters (AuNCs-MLFIA sensor) was successfully established for the simultaneous and quantitative determination of clenbuterol (Clen) and ractopamine (RAC) in swine urine. The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l-arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor. The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment, qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L−1. In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L−1, respectively, with detection limits of 0.003 and 0.023 μg L−1. Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes, and single-analyte detection. Multiplex LFIA sensor based on highly luminescent green-emitting gold nanoclusters for simultaneous and quantitative determination of Clen and RAC in swine urine. [Display omitted] •Highly luminescent green-emitting AuNCs were applied to LFIA for the first time.•AuNCs-MLFIA sensor was developed for simultaneous and quantitative determination of Clen and RAC in swine urine.•The developed AuNCs-MLFIA sensor revealed superior in sensitivity than other LFIA sensors.
doi_str_mv 10.1016/j.aca.2018.08.014
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The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l-arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor. The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment, qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L−1. In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L−1, respectively, with detection limits of 0.003 and 0.023 μg L−1. Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes, and single-analyte detection. Multiplex LFIA sensor based on highly luminescent green-emitting gold nanoclusters for simultaneous and quantitative determination of Clen and RAC in swine urine. [Display omitted] •Highly luminescent green-emitting AuNCs were applied to LFIA for the first time.•AuNCs-MLFIA sensor was developed for simultaneous and quantitative determination of Clen and RAC in swine urine.•The developed AuNCs-MLFIA sensor revealed superior in sensitivity than other LFIA sensors.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2018.08.014</identifier><identifier>PMID: 30327104</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antigens ; Arg/ATT/AuNCs ; Arginine ; AuNCs-MLFIA sensor ; Biomolecules ; Biosensing Techniques ; Biosensors ; Cellulose esters ; Cellulose nitrate ; Chromatography, Liquid ; Clenbuterol ; Clenbuterol - urine ; Conjugation ; Detection limits ; Drugs ; Feeds ; Fluorescence ; Gold ; Gold - chemistry ; Immunoassay ; Inspection ; Liquid chromatography ; Livestock ; Luminescence ; Mass spectrometry ; Mass spectroscopy ; Metal Nanoparticles - chemistry ; Multiplex detection ; Multiplexing ; Nanoclusters ; Nanoparticles ; Phenethylamines - urine ; Pretreatment ; Ractopamine ; Sensitivity analysis ; Sensors ; Swine ; Tandem Mass Spectrometry ; Urine</subject><ispartof>Analytica chimica acta, 2018-12, Vol.1040, p.143-149</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. 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The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l-arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor. The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment, qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L−1. In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L−1, respectively, with detection limits of 0.003 and 0.023 μg L−1. Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes, and single-analyte detection. Multiplex LFIA sensor based on highly luminescent green-emitting gold nanoclusters for simultaneous and quantitative determination of Clen and RAC in swine urine. 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The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l-arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor. The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment, qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L−1. In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L−1, respectively, with detection limits of 0.003 and 0.023 μg L−1. Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes, and single-analyte detection. Multiplex LFIA sensor based on highly luminescent green-emitting gold nanoclusters for simultaneous and quantitative determination of Clen and RAC in swine urine. [Display omitted] •Highly luminescent green-emitting AuNCs were applied to LFIA for the first time.•AuNCs-MLFIA sensor was developed for simultaneous and quantitative determination of Clen and RAC in swine urine.•The developed AuNCs-MLFIA sensor revealed superior in sensitivity than other LFIA sensors.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30327104</pmid><doi>10.1016/j.aca.2018.08.014</doi><tpages>7</tpages></addata></record>
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subjects Animals
Antigens
Arg/ATT/AuNCs
Arginine
AuNCs-MLFIA sensor
Biomolecules
Biosensing Techniques
Biosensors
Cellulose esters
Cellulose nitrate
Chromatography, Liquid
Clenbuterol
Clenbuterol - urine
Conjugation
Detection limits
Drugs
Feeds
Fluorescence
Gold
Gold - chemistry
Immunoassay
Inspection
Liquid chromatography
Livestock
Luminescence
Mass spectrometry
Mass spectroscopy
Metal Nanoparticles - chemistry
Multiplex detection
Multiplexing
Nanoclusters
Nanoparticles
Phenethylamines - urine
Pretreatment
Ractopamine
Sensitivity analysis
Sensors
Swine
Tandem Mass Spectrometry
Urine
title Highly luminescent green-emitting Au nanocluster-based multiplex lateral flow immunoassay for ultrasensitive detection of clenbuterol and ractopamine
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