Highly luminescent green-emitting Au nanocluster-based multiplex lateral flow immunoassay for ultrasensitive detection of clenbuterol and ractopamine
A multiplex lateral flow immunoassay sensor based on highly luminescent green-emitting Au nanoclusters (AuNCs-MLFIA sensor) was successfully established for the simultaneous and quantitative determination of clenbuterol (Clen) and ractopamine (RAC) in swine urine. The antigens of Clen and RAC were d...
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description | A multiplex lateral flow immunoassay sensor based on highly luminescent green-emitting Au nanoclusters (AuNCs-MLFIA sensor) was successfully established for the simultaneous and quantitative determination of clenbuterol (Clen) and ractopamine (RAC) in swine urine. The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l-arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor. The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment, qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L−1. In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L−1, respectively, with detection limits of 0.003 and 0.023 μg L−1. Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes, and single-analyte detection.
Multiplex LFIA sensor based on highly luminescent green-emitting gold nanoclusters for simultaneous and quantitative determination of Clen and RAC in swine urine. [Display omitted]
•Highly luminescent green-emitting AuNCs were applied to LFIA for the first time.•AuNCs-MLFIA sensor was developed for simultaneous and quantitative determination of Clen and RAC in swine urine.•The developed AuNCs-MLFIA sensor revealed superior in sensitivity than other LFIA sensors. |
doi_str_mv | 10.1016/j.aca.2018.08.014 |
format | Article |
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Multiplex LFIA sensor based on highly luminescent green-emitting gold nanoclusters for simultaneous and quantitative determination of Clen and RAC in swine urine. [Display omitted]
•Highly luminescent green-emitting AuNCs were applied to LFIA for the first time.•AuNCs-MLFIA sensor was developed for simultaneous and quantitative determination of Clen and RAC in swine urine.•The developed AuNCs-MLFIA sensor revealed superior in sensitivity than other LFIA sensors.</description><identifier>ISSN: 0003-2670</identifier><identifier>EISSN: 1873-4324</identifier><identifier>DOI: 10.1016/j.aca.2018.08.014</identifier><identifier>PMID: 30327104</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Antigens ; Arg/ATT/AuNCs ; Arginine ; AuNCs-MLFIA sensor ; Biomolecules ; Biosensing Techniques ; Biosensors ; Cellulose esters ; Cellulose nitrate ; Chromatography, Liquid ; Clenbuterol ; Clenbuterol - urine ; Conjugation ; Detection limits ; Drugs ; Feeds ; Fluorescence ; Gold ; Gold - chemistry ; Immunoassay ; Inspection ; Liquid chromatography ; Livestock ; Luminescence ; Mass spectrometry ; Mass spectroscopy ; Metal Nanoparticles - chemistry ; Multiplex detection ; Multiplexing ; Nanoclusters ; Nanoparticles ; Phenethylamines - urine ; Pretreatment ; Ractopamine ; Sensitivity analysis ; Sensors ; Swine ; Tandem Mass Spectrometry ; Urine</subject><ispartof>Analytica chimica acta, 2018-12, Vol.1040, p.143-149</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><rights>Copyright Elsevier BV Dec 21, 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c381t-f6fcbdd8139262db928f0c85d3830674bf1883e66730493856388c909625e0f73</citedby><cites>FETCH-LOGICAL-c381t-f6fcbdd8139262db928f0c85d3830674bf1883e66730493856388c909625e0f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.aca.2018.08.014$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30327104$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Peng, Tao</creatorcontrib><creatorcontrib>Wang, Jianyi</creatorcontrib><creatorcontrib>Zhao, Sijun</creatorcontrib><creatorcontrib>Zeng, Yuyang</creatorcontrib><creatorcontrib>Zheng, Pimiao</creatorcontrib><creatorcontrib>Liang, Demei</creatorcontrib><creatorcontrib>Mari, Ghulam Mujtaba</creatorcontrib><creatorcontrib>Jiang, Haiyang</creatorcontrib><title>Highly luminescent green-emitting Au nanocluster-based multiplex lateral flow immunoassay for ultrasensitive detection of clenbuterol and ractopamine</title><title>Analytica chimica acta</title><addtitle>Anal Chim Acta</addtitle><description>A multiplex lateral flow immunoassay sensor based on highly luminescent green-emitting Au nanoclusters (AuNCs-MLFIA sensor) was successfully established for the simultaneous and quantitative determination of clenbuterol (Clen) and ractopamine (RAC) in swine urine. The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l-arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor. The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment, qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L−1. In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L−1, respectively, with detection limits of 0.003 and 0.023 μg L−1. Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes, and single-analyte detection.
Multiplex LFIA sensor based on highly luminescent green-emitting gold nanoclusters for simultaneous and quantitative determination of Clen and RAC in swine urine. [Display omitted]
•Highly luminescent green-emitting AuNCs were applied to LFIA for the first time.•AuNCs-MLFIA sensor was developed for simultaneous and quantitative determination of Clen and RAC in swine urine.•The developed AuNCs-MLFIA sensor revealed superior in sensitivity than other LFIA sensors.</description><subject>Animals</subject><subject>Antigens</subject><subject>Arg/ATT/AuNCs</subject><subject>Arginine</subject><subject>AuNCs-MLFIA sensor</subject><subject>Biomolecules</subject><subject>Biosensing Techniques</subject><subject>Biosensors</subject><subject>Cellulose esters</subject><subject>Cellulose nitrate</subject><subject>Chromatography, Liquid</subject><subject>Clenbuterol</subject><subject>Clenbuterol - urine</subject><subject>Conjugation</subject><subject>Detection limits</subject><subject>Drugs</subject><subject>Feeds</subject><subject>Fluorescence</subject><subject>Gold</subject><subject>Gold - chemistry</subject><subject>Immunoassay</subject><subject>Inspection</subject><subject>Liquid chromatography</subject><subject>Livestock</subject><subject>Luminescence</subject><subject>Mass spectrometry</subject><subject>Mass spectroscopy</subject><subject>Metal Nanoparticles - chemistry</subject><subject>Multiplex detection</subject><subject>Multiplexing</subject><subject>Nanoclusters</subject><subject>Nanoparticles</subject><subject>Phenethylamines - urine</subject><subject>Pretreatment</subject><subject>Ractopamine</subject><subject>Sensitivity analysis</subject><subject>Sensors</subject><subject>Swine</subject><subject>Tandem Mass Spectrometry</subject><subject>Urine</subject><issn>0003-2670</issn><issn>1873-4324</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kUuLFDEUhYMoTs_oD3AjATduqs2jHilcDYM6woAbXYdUctOmSSVtHqP9Q_y_pukZFy6EC3nwncPlHIReUbKlhI7v9lul1ZYRKrakDe2foA0VE-96zvqnaEMI4R0bJ3KBLnPetyejpH-OLjjhbGrXDfp963bf_RH7uroAWUMoeJcAQgerK8WFHb6uOKgQta-5QOoWlcHgtfriDh5-Ya_ar_LY-vgTu3WtIaqc1RHbmHCjUuNDdsXdAzZQQBcXA44Waw9hqU0cPVbB4KR0iQd12uMFemaVz_Dy4bxC3z5--Hpz2919-fT55vqu01zQ0tnR6sUYQfnMRmaWmQlLtBgMF5yMU79YKgSHcZw46WcuhpELoWcyj2wAYid-hd6efQ8p_qiQi1xdy8B7FSDWLBlltBdioKKhb_5B97Gm0LZr1MCbI-O0UfRM6RRzTmDlIblVpaOkRJ46k3vZOpOnziRpQ_umef3gXJcVzF_FY0kNeH8GoEVx7yDJrB0EDcalFqc00f3H_g-jL6lS</recordid><startdate>20181221</startdate><enddate>20181221</enddate><creator>Peng, Tao</creator><creator>Wang, Jianyi</creator><creator>Zhao, Sijun</creator><creator>Zeng, Yuyang</creator><creator>Zheng, Pimiao</creator><creator>Liang, Demei</creator><creator>Mari, Ghulam Mujtaba</creator><creator>Jiang, Haiyang</creator><general>Elsevier B.V</general><general>Elsevier BV</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7T7</scope><scope>7TA</scope><scope>7TB</scope><scope>7TK</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20181221</creationdate><title>Highly luminescent green-emitting Au nanocluster-based multiplex lateral flow immunoassay for ultrasensitive detection of clenbuterol and ractopamine</title><author>Peng, Tao ; Wang, Jianyi ; Zhao, Sijun ; Zeng, Yuyang ; Zheng, Pimiao ; Liang, Demei ; Mari, Ghulam Mujtaba ; Jiang, Haiyang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c381t-f6fcbdd8139262db928f0c85d3830674bf1883e66730493856388c909625e0f73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Antigens</topic><topic>Arg/ATT/AuNCs</topic><topic>Arginine</topic><topic>AuNCs-MLFIA sensor</topic><topic>Biomolecules</topic><topic>Biosensing Techniques</topic><topic>Biosensors</topic><topic>Cellulose esters</topic><topic>Cellulose nitrate</topic><topic>Chromatography, Liquid</topic><topic>Clenbuterol</topic><topic>Clenbuterol - urine</topic><topic>Conjugation</topic><topic>Detection limits</topic><topic>Drugs</topic><topic>Feeds</topic><topic>Fluorescence</topic><topic>Gold</topic><topic>Gold - chemistry</topic><topic>Immunoassay</topic><topic>Inspection</topic><topic>Liquid chromatography</topic><topic>Livestock</topic><topic>Luminescence</topic><topic>Mass spectrometry</topic><topic>Mass spectroscopy</topic><topic>Metal Nanoparticles - chemistry</topic><topic>Multiplex detection</topic><topic>Multiplexing</topic><topic>Nanoclusters</topic><topic>Nanoparticles</topic><topic>Phenethylamines - urine</topic><topic>Pretreatment</topic><topic>Ractopamine</topic><topic>Sensitivity analysis</topic><topic>Sensors</topic><topic>Swine</topic><topic>Tandem Mass Spectrometry</topic><topic>Urine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Peng, Tao</creatorcontrib><creatorcontrib>Wang, Jianyi</creatorcontrib><creatorcontrib>Zhao, Sijun</creatorcontrib><creatorcontrib>Zeng, Yuyang</creatorcontrib><creatorcontrib>Zheng, Pimiao</creatorcontrib><creatorcontrib>Liang, Demei</creatorcontrib><creatorcontrib>Mari, Ghulam Mujtaba</creatorcontrib><creatorcontrib>Jiang, Haiyang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytica chimica acta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Peng, Tao</au><au>Wang, Jianyi</au><au>Zhao, Sijun</au><au>Zeng, Yuyang</au><au>Zheng, Pimiao</au><au>Liang, Demei</au><au>Mari, Ghulam Mujtaba</au><au>Jiang, Haiyang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Highly luminescent green-emitting Au nanocluster-based multiplex lateral flow immunoassay for ultrasensitive detection of clenbuterol and ractopamine</atitle><jtitle>Analytica chimica acta</jtitle><addtitle>Anal Chim Acta</addtitle><date>2018-12-21</date><risdate>2018</risdate><volume>1040</volume><spage>143</spage><epage>149</epage><pages>143-149</pages><issn>0003-2670</issn><eissn>1873-4324</eissn><abstract>A multiplex lateral flow immunoassay sensor based on highly luminescent green-emitting Au nanoclusters (AuNCs-MLFIA sensor) was successfully established for the simultaneous and quantitative determination of clenbuterol (Clen) and ractopamine (RAC) in swine urine. The antigens of Clen and RAC were dispersed on a nitrocellulose membrane as two test lines, and the Au nanoclusters were synthesized from 6-aza-2-thiothymine and l-arginine to obtain highly green luminescence and ultra-small nanoparticles (Arg/ATT/AuNCs). Free carboxyl groups on Arg/ATT/AuNCs enabled conjugation with biomolecules to afford an indicator for the biosensor. The AuNCs-MLFIA sensor is based on the indirect competition assay and could successfully detect samples within 18 min without sample pretreatment, qualitative results can be obtained by visual inspection under a UV lamp. The limits of detection of Clen and RAC by the naked eye were both 0.25 μg L−1. In addition, the AuNCs-MLFIA sensor allowed quantitative detection combined with a portable fluorescence reader. The half-maximal inhibitory concentrations of Clen and RAC were 0.06 and 0.32 μg L−1, respectively, with detection limits of 0.003 and 0.023 μg L−1. Thirty blind-spiked swine urine samples were analyzed by the AuNCs-MLFIA sensor and liquid chromatography–tandem mass spectrometry, and the results of the two methods showed a significant correlation. The newly developed AuNCs-MLFIA sensor overcomes several limitations of conventional LFIA sensors, including their low sensitivity, limitation to quantify analytes, and single-analyte detection.
Multiplex LFIA sensor based on highly luminescent green-emitting gold nanoclusters for simultaneous and quantitative determination of Clen and RAC in swine urine. [Display omitted]
•Highly luminescent green-emitting AuNCs were applied to LFIA for the first time.•AuNCs-MLFIA sensor was developed for simultaneous and quantitative determination of Clen and RAC in swine urine.•The developed AuNCs-MLFIA sensor revealed superior in sensitivity than other LFIA sensors.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30327104</pmid><doi>10.1016/j.aca.2018.08.014</doi><tpages>7</tpages></addata></record> |
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subjects | Animals Antigens Arg/ATT/AuNCs Arginine AuNCs-MLFIA sensor Biomolecules Biosensing Techniques Biosensors Cellulose esters Cellulose nitrate Chromatography, Liquid Clenbuterol Clenbuterol - urine Conjugation Detection limits Drugs Feeds Fluorescence Gold Gold - chemistry Immunoassay Inspection Liquid chromatography Livestock Luminescence Mass spectrometry Mass spectroscopy Metal Nanoparticles - chemistry Multiplex detection Multiplexing Nanoclusters Nanoparticles Phenethylamines - urine Pretreatment Ractopamine Sensitivity analysis Sensors Swine Tandem Mass Spectrometry Urine |
title | Highly luminescent green-emitting Au nanocluster-based multiplex lateral flow immunoassay for ultrasensitive detection of clenbuterol and ractopamine |
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