Proteomic analysis of indium embryotoxicity in cultured postimplantation rat embryos
Indium embryotoxicity was investigated by proteomic analysis with two-dimensional electrophoresis of rat embryos cultured from day 10.5 of gestation for 24 h in the presence of 50 μM indium trichloride. In the embryo proper, indium increased quantity of several protein spots including those identifi...
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| Veröffentlicht in: | Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2009-12, Vol.28 (4), p.477-488 |
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| container_title | Reproductive toxicology (Elmsford, N.Y.) |
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| creator | Usami, Makoto Nakajima, Mikio Mitsunaga, Katsuyoshi Miyajima, Atsuko Sunouchi, Momoko Doi, Osamu |
| description | Indium embryotoxicity was investigated by proteomic analysis with two-dimensional electrophoresis of rat embryos cultured from day 10.5 of gestation for 24
h in the presence of 50
μM indium trichloride. In the embryo proper, indium increased quantity of several protein spots including those identified as serum albumin, phosphorylated cofilin 1, phosphorylated destrin and tyrosyl-tRNA synthetase. The increased serum albumin, derived from the culture medium composed of rat serum, may decrease the toxicity of indium. The increase of phosphorylated cofilin 1 might be involved in dysmorphogenicity of indium through perturbation of actin functions. In the yolk sac membrane, indium induced quantitative and qualitative changes in the protein spots. Proteins from appeared spots included stress proteins, and those from decreased or disappeared spots included serum proteins, glycolytic pathway enzymes and cytoskeletal proteins, indicating yolk sac dysfunction. Thus, several candidate proteins that might be involved in indium embryotoxicity were identified. |
| doi_str_mv | 10.1016/j.reprotox.2009.06.008 |
| format | Article |
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h in the presence of 50
μM indium trichloride. In the embryo proper, indium increased quantity of several protein spots including those identified as serum albumin, phosphorylated cofilin 1, phosphorylated destrin and tyrosyl-tRNA synthetase. The increased serum albumin, derived from the culture medium composed of rat serum, may decrease the toxicity of indium. The increase of phosphorylated cofilin 1 might be involved in dysmorphogenicity of indium through perturbation of actin functions. In the yolk sac membrane, indium induced quantitative and qualitative changes in the protein spots. Proteins from appeared spots included stress proteins, and those from decreased or disappeared spots included serum proteins, glycolytic pathway enzymes and cytoskeletal proteins, indicating yolk sac dysfunction. Thus, several candidate proteins that might be involved in indium embryotoxicity were identified.</description><identifier>ISSN: 0890-6238</identifier><identifier>EISSN: 1873-1708</identifier><identifier>DOI: 10.1016/j.reprotox.2009.06.008</identifier><identifier>PMID: 19539025</identifier><language>eng</language><publisher>Amsterdam: Elsevier Inc</publisher><subject>2-DE ; Animals ; Biological and medical sciences ; Cofilin 1 - metabolism ; Destrin - metabolism ; Dose-Response Relationship, Drug ; Egg Proteins - drug effects ; Egg Proteins - metabolism ; Embryo Culture Techniques ; Embryo, Mammalian - drug effects ; Embryo, Mammalian - metabolism ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic Development - drug effects ; Embryotoxicity ; Female ; Fundamental and applied biological sciences. Psychology ; Indium ; Indium - pharmacology ; Indium - toxicity ; Mass spectrometry ; Medical sciences ; Membrane Proteins - drug effects ; Membrane Proteins - metabolism ; Organ Culture Techniques ; Pregnancy ; Proteome - analysis ; Proteome - drug effects ; Proteomics ; Rat ; Rats ; Rats, Wistar ; Teratology. Teratogens ; Toxicology ; Yolk Sac - chemistry ; Yolk Sac - drug effects</subject><ispartof>Reproductive toxicology (Elmsford, N.Y.), 2009-12, Vol.28 (4), p.477-488</ispartof><rights>2009 Elsevier Inc.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-d183cdc25bfa20fe85caf0020e32b244bff422a4c6d2073fae4a0214a454d1ba3</citedby><cites>FETCH-LOGICAL-c494t-d183cdc25bfa20fe85caf0020e32b244bff422a4c6d2073fae4a0214a454d1ba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.reprotox.2009.06.008$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22149323$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19539025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Usami, Makoto</creatorcontrib><creatorcontrib>Nakajima, Mikio</creatorcontrib><creatorcontrib>Mitsunaga, Katsuyoshi</creatorcontrib><creatorcontrib>Miyajima, Atsuko</creatorcontrib><creatorcontrib>Sunouchi, Momoko</creatorcontrib><creatorcontrib>Doi, Osamu</creatorcontrib><title>Proteomic analysis of indium embryotoxicity in cultured postimplantation rat embryos</title><title>Reproductive toxicology (Elmsford, N.Y.)</title><addtitle>Reprod Toxicol</addtitle><description>Indium embryotoxicity was investigated by proteomic analysis with two-dimensional electrophoresis of rat embryos cultured from day 10.5 of gestation for 24
h in the presence of 50
μM indium trichloride. In the embryo proper, indium increased quantity of several protein spots including those identified as serum albumin, phosphorylated cofilin 1, phosphorylated destrin and tyrosyl-tRNA synthetase. The increased serum albumin, derived from the culture medium composed of rat serum, may decrease the toxicity of indium. The increase of phosphorylated cofilin 1 might be involved in dysmorphogenicity of indium through perturbation of actin functions. In the yolk sac membrane, indium induced quantitative and qualitative changes in the protein spots. Proteins from appeared spots included stress proteins, and those from decreased or disappeared spots included serum proteins, glycolytic pathway enzymes and cytoskeletal proteins, indicating yolk sac dysfunction. Thus, several candidate proteins that might be involved in indium embryotoxicity were identified.</description><subject>2-DE</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cofilin 1 - metabolism</subject><subject>Destrin - metabolism</subject><subject>Dose-Response Relationship, Drug</subject><subject>Egg Proteins - drug effects</subject><subject>Egg Proteins - metabolism</subject><subject>Embryo Culture Techniques</subject><subject>Embryo, Mammalian - drug effects</subject><subject>Embryo, Mammalian - metabolism</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic Development - drug effects</subject><subject>Embryotoxicity</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Indium</subject><subject>Indium - pharmacology</subject><subject>Indium - toxicity</subject><subject>Mass spectrometry</subject><subject>Medical sciences</subject><subject>Membrane Proteins - drug effects</subject><subject>Membrane Proteins - metabolism</subject><subject>Organ Culture Techniques</subject><subject>Pregnancy</subject><subject>Proteome - analysis</subject><subject>Proteome - drug effects</subject><subject>Proteomics</subject><subject>Rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Teratology. Teratogens</subject><subject>Toxicology</subject><subject>Yolk Sac - chemistry</subject><subject>Yolk Sac - drug effects</subject><issn>0890-6238</issn><issn>1873-1708</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE2LFDEQhoMo7rj6F5a-6K3bykd_3ZTFL1jQw3oO1UkFMnR3xiQtzr83w7R69FRQPG_Vy8PYHYeGA-_eHptIpxhy-NUIgLGBrgEYnrADH3pZ8x6Gp-wAwwh1J-Rww16kdAQA1Y_9c3bDx1aOINoDe_xWjlBYvKlwxfmcfKqCq_xq_bZUtEzxfHnijc_nsq3MNuctkq1OIWW_nGZcM2Yf1ipi3vn0kj1zOCd6tc9b9v3jh8f7z_XD109f7t8_1EaNKteWD9JYI9rJoQBHQ2vQAQggKSah1OScEgKV6ayAXjokhSC4QtUqyyeUt-zN9W4x8WOjlPXik6G5lKKwJS244BI6VcDuCpoYUork9Cn6BeNZc9AXn_qo__jUF58aOl18luDd_mGbFrL_YrvAArzeAUwGZxdxNT795USpO0ohC_fuylHx8dNT1Ml4Wg1ZH8lkbYP_X5ffkWSaJw</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Usami, Makoto</creator><creator>Nakajima, Mikio</creator><creator>Mitsunaga, Katsuyoshi</creator><creator>Miyajima, Atsuko</creator><creator>Sunouchi, Momoko</creator><creator>Doi, Osamu</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7ST</scope><scope>7U7</scope><scope>C1K</scope><scope>SOI</scope></search><sort><creationdate>20091201</creationdate><title>Proteomic analysis of indium embryotoxicity in cultured postimplantation rat embryos</title><author>Usami, Makoto ; Nakajima, Mikio ; Mitsunaga, Katsuyoshi ; Miyajima, Atsuko ; Sunouchi, Momoko ; Doi, Osamu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c494t-d183cdc25bfa20fe85caf0020e32b244bff422a4c6d2073fae4a0214a454d1ba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>2-DE</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cofilin 1 - metabolism</topic><topic>Destrin - metabolism</topic><topic>Dose-Response Relationship, Drug</topic><topic>Egg Proteins - drug effects</topic><topic>Egg Proteins - metabolism</topic><topic>Embryo Culture Techniques</topic><topic>Embryo, Mammalian - drug effects</topic><topic>Embryo, Mammalian - metabolism</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic Development - drug effects</topic><topic>Embryotoxicity</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Indium</topic><topic>Indium - pharmacology</topic><topic>Indium - toxicity</topic><topic>Mass spectrometry</topic><topic>Medical sciences</topic><topic>Membrane Proteins - drug effects</topic><topic>Membrane Proteins - metabolism</topic><topic>Organ Culture Techniques</topic><topic>Pregnancy</topic><topic>Proteome - analysis</topic><topic>Proteome - drug effects</topic><topic>Proteomics</topic><topic>Rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Teratology. Teratogens</topic><topic>Toxicology</topic><topic>Yolk Sac - chemistry</topic><topic>Yolk Sac - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Usami, Makoto</creatorcontrib><creatorcontrib>Nakajima, Mikio</creatorcontrib><creatorcontrib>Mitsunaga, Katsuyoshi</creatorcontrib><creatorcontrib>Miyajima, Atsuko</creatorcontrib><creatorcontrib>Sunouchi, Momoko</creatorcontrib><creatorcontrib>Doi, Osamu</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Environment Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>Reproductive toxicology (Elmsford, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Usami, Makoto</au><au>Nakajima, Mikio</au><au>Mitsunaga, Katsuyoshi</au><au>Miyajima, Atsuko</au><au>Sunouchi, Momoko</au><au>Doi, Osamu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteomic analysis of indium embryotoxicity in cultured postimplantation rat embryos</atitle><jtitle>Reproductive toxicology (Elmsford, N.Y.)</jtitle><addtitle>Reprod Toxicol</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>28</volume><issue>4</issue><spage>477</spage><epage>488</epage><pages>477-488</pages><issn>0890-6238</issn><eissn>1873-1708</eissn><abstract>Indium embryotoxicity was investigated by proteomic analysis with two-dimensional electrophoresis of rat embryos cultured from day 10.5 of gestation for 24
h in the presence of 50
μM indium trichloride. In the embryo proper, indium increased quantity of several protein spots including those identified as serum albumin, phosphorylated cofilin 1, phosphorylated destrin and tyrosyl-tRNA synthetase. The increased serum albumin, derived from the culture medium composed of rat serum, may decrease the toxicity of indium. The increase of phosphorylated cofilin 1 might be involved in dysmorphogenicity of indium through perturbation of actin functions. In the yolk sac membrane, indium induced quantitative and qualitative changes in the protein spots. Proteins from appeared spots included stress proteins, and those from decreased or disappeared spots included serum proteins, glycolytic pathway enzymes and cytoskeletal proteins, indicating yolk sac dysfunction. Thus, several candidate proteins that might be involved in indium embryotoxicity were identified.</abstract><cop>Amsterdam</cop><pub>Elsevier Inc</pub><pmid>19539025</pmid><doi>10.1016/j.reprotox.2009.06.008</doi><tpages>12</tpages></addata></record> |
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| subjects | 2-DE Animals Biological and medical sciences Cofilin 1 - metabolism Destrin - metabolism Dose-Response Relationship, Drug Egg Proteins - drug effects Egg Proteins - metabolism Embryo Culture Techniques Embryo, Mammalian - drug effects Embryo, Mammalian - metabolism Embryology: invertebrates and vertebrates. Teratology Embryonic Development - drug effects Embryotoxicity Female Fundamental and applied biological sciences. Psychology Indium Indium - pharmacology Indium - toxicity Mass spectrometry Medical sciences Membrane Proteins - drug effects Membrane Proteins - metabolism Organ Culture Techniques Pregnancy Proteome - analysis Proteome - drug effects Proteomics Rat Rats Rats, Wistar Teratology. Teratogens Toxicology Yolk Sac - chemistry Yolk Sac - drug effects |
| title | Proteomic analysis of indium embryotoxicity in cultured postimplantation rat embryos |
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