miR‐199b‐5p‐Stonin 2 axis regulates metastases and epithelial‐to‐mesenchymal transition of papillary thyroid carcinoma
Papillary thyroid carcinoma is one of the most fatal malignant endocrine tumors, and the prognosis remains poor because of the lack of effective therapeutic targets. In this study, we demonstrated that the level of miR‐199b‐5p was markedly downregulated in papillary thyroid carcinoma. The ectopic ex...
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| Veröffentlicht in: | IUBMB life 2019-01, Vol.71 (1), p.28-40 |
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| description | Papillary thyroid carcinoma is one of the most fatal malignant endocrine tumors, and the prognosis remains poor because of the lack of effective therapeutic targets. In this study, we demonstrated that the level of miR‐199b‐5p was markedly downregulated in papillary thyroid carcinoma. The ectopic expression level of miR‐199b‐5p in papillary thyroid carcinoma cell B‐CPAP could inhibit growth, migration, and invasion as well as epithelial‐mesenchymal transition (EMT) and decreased cell metastasis in vivo, but silencing miR‐199b‐5p caused a contradictory outcome. Additionally, Stonin 2 (STON2) was identified as a direct target gene of miR‐199b‐5p. Consistent with the downregulation of miR‐199b‐5p, the overexpression of STON2 induced the growth, migration and invasion of B‐CPAP cells. It was also demonstrated that miR‐199b‐5p suppressed papillary thyroid carcinoma cell aggressiveness by targeting STON2. Furthermore, the overexpression of miR‐199b‐5p inhibited cell proliferation, promoted apoptosis, and increased the chemo‐sensitivity of thyroid carcinoma B‐CPAP cells toward the chemotherapy drug paclitaxel. Finally, in vivo experiments further demonstrated that miR‐199b‐5p suppressed tumor growth in nude mice. Thus, this study revealed that miR‐199b‐5p functions as antioncogene miRNA in papillary thyroid carcinoma cells and that the miR‐199b‐5p/STON2 axis might be a potential treatment option for papillary thyroid carcinoma. © 2018 IUBMB Life, 71(1):28–40, 2019 |
| doi_str_mv | 10.1002/iub.1889 |
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In this study, we demonstrated that the level of miR‐199b‐5p was markedly downregulated in papillary thyroid carcinoma. The ectopic expression level of miR‐199b‐5p in papillary thyroid carcinoma cell B‐CPAP could inhibit growth, migration, and invasion as well as epithelial‐mesenchymal transition (EMT) and decreased cell metastasis in vivo, but silencing miR‐199b‐5p caused a contradictory outcome. Additionally, Stonin 2 (STON2) was identified as a direct target gene of miR‐199b‐5p. Consistent with the downregulation of miR‐199b‐5p, the overexpression of STON2 induced the growth, migration and invasion of B‐CPAP cells. It was also demonstrated that miR‐199b‐5p suppressed papillary thyroid carcinoma cell aggressiveness by targeting STON2. Furthermore, the overexpression of miR‐199b‐5p inhibited cell proliferation, promoted apoptosis, and increased the chemo‐sensitivity of thyroid carcinoma B‐CPAP cells toward the chemotherapy drug paclitaxel. Finally, in vivo experiments further demonstrated that miR‐199b‐5p suppressed tumor growth in nude mice. Thus, this study revealed that miR‐199b‐5p functions as antioncogene miRNA in papillary thyroid carcinoma cells and that the miR‐199b‐5p/STON2 axis might be a potential treatment option for papillary thyroid carcinoma. © 2018 IUBMB Life, 71(1):28–40, 2019</description><identifier>ISSN: 1521-6543</identifier><identifier>EISSN: 1521-6551</identifier><identifier>DOI: 10.1002/iub.1889</identifier><identifier>PMID: 30325582</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Adaptor Proteins, Vesicular Transport - genetics ; Animals ; Apoptosis ; Cell Line, Tumor ; Cell Movement - genetics ; Cell proliferation ; Cell Proliferation - genetics ; Chemotherapy ; Chromosome 5 ; Ectopic expression ; Epithelial-Mesenchymal Transition - genetics ; Gene Expression Regulation, Neoplastic ; Heterografts ; Humans ; Lymphocytes B ; Medicare ; Mesenchyme ; Metastases ; metastasis ; Mice ; MicroRNAs - genetics ; miRNA ; miR‐199b‐5p ; Paclitaxel ; Paclitaxel - pharmacology ; Papillary thyroid carcinoma ; Prognosis ; Reimbursement ; STON2 ; Therapeutic applications ; Thyroid ; Thyroid cancer ; Thyroid Cancer, Papillary - drug therapy ; Thyroid Cancer, Papillary - genetics ; Thyroid Cancer, Papillary - pathology ; Tomography ; Tumors</subject><ispartof>IUBMB life, 2019-01, Vol.71 (1), p.28-40</ispartof><rights>2018 International Union of Biochemistry and Molecular Biology</rights><rights>2018 International Union of Biochemistry and Molecular Biology.</rights><rights>2019 International Union of Biochemistry and Molecular Biology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3839-6fd10d90da1e1aea0c41f94a6cd5571d0ec61b9dbecb1b5e293675f0eb8b4e833</citedby><cites>FETCH-LOGICAL-c3839-6fd10d90da1e1aea0c41f94a6cd5571d0ec61b9dbecb1b5e293675f0eb8b4e833</cites><orcidid>0000-0001-9504-1099</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fiub.1889$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fiub.1889$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,1427,27903,27904,45553,45554,46388,46812</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30325582$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ren, Lei</creatorcontrib><creatorcontrib>Xu, Yapei</creatorcontrib><creatorcontrib>Qin, Guijun</creatorcontrib><creatorcontrib>Liu, Cong</creatorcontrib><creatorcontrib>Yan, Yushan</creatorcontrib><creatorcontrib>Zhang, Huijuan</creatorcontrib><title>miR‐199b‐5p‐Stonin 2 axis regulates metastases and epithelial‐to‐mesenchymal transition of papillary thyroid carcinoma</title><title>IUBMB life</title><addtitle>IUBMB Life</addtitle><description>Papillary thyroid carcinoma is one of the most fatal malignant endocrine tumors, and the prognosis remains poor because of the lack of effective therapeutic targets. In this study, we demonstrated that the level of miR‐199b‐5p was markedly downregulated in papillary thyroid carcinoma. The ectopic expression level of miR‐199b‐5p in papillary thyroid carcinoma cell B‐CPAP could inhibit growth, migration, and invasion as well as epithelial‐mesenchymal transition (EMT) and decreased cell metastasis in vivo, but silencing miR‐199b‐5p caused a contradictory outcome. Additionally, Stonin 2 (STON2) was identified as a direct target gene of miR‐199b‐5p. Consistent with the downregulation of miR‐199b‐5p, the overexpression of STON2 induced the growth, migration and invasion of B‐CPAP cells. It was also demonstrated that miR‐199b‐5p suppressed papillary thyroid carcinoma cell aggressiveness by targeting STON2. Furthermore, the overexpression of miR‐199b‐5p inhibited cell proliferation, promoted apoptosis, and increased the chemo‐sensitivity of thyroid carcinoma B‐CPAP cells toward the chemotherapy drug paclitaxel. Finally, in vivo experiments further demonstrated that miR‐199b‐5p suppressed tumor growth in nude mice. Thus, this study revealed that miR‐199b‐5p functions as antioncogene miRNA in papillary thyroid carcinoma cells and that the miR‐199b‐5p/STON2 axis might be a potential treatment option for papillary thyroid carcinoma. © 2018 IUBMB Life, 71(1):28–40, 2019</description><subject>Adaptor Proteins, Vesicular Transport - genetics</subject><subject>Animals</subject><subject>Apoptosis</subject><subject>Cell Line, Tumor</subject><subject>Cell Movement - genetics</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - genetics</subject><subject>Chemotherapy</subject><subject>Chromosome 5</subject><subject>Ectopic expression</subject><subject>Epithelial-Mesenchymal Transition - genetics</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Heterografts</subject><subject>Humans</subject><subject>Lymphocytes B</subject><subject>Medicare</subject><subject>Mesenchyme</subject><subject>Metastases</subject><subject>metastasis</subject><subject>Mice</subject><subject>MicroRNAs - genetics</subject><subject>miRNA</subject><subject>miR‐199b‐5p</subject><subject>Paclitaxel</subject><subject>Paclitaxel - pharmacology</subject><subject>Papillary thyroid carcinoma</subject><subject>Prognosis</subject><subject>Reimbursement</subject><subject>STON2</subject><subject>Therapeutic applications</subject><subject>Thyroid</subject><subject>Thyroid cancer</subject><subject>Thyroid Cancer, Papillary - drug therapy</subject><subject>Thyroid Cancer, Papillary - genetics</subject><subject>Thyroid Cancer, Papillary - pathology</subject><subject>Tomography</subject><subject>Tumors</subject><issn>1521-6543</issn><issn>1521-6551</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc9KHTEUh0OxVGsLfQIJuOlmNGdmMney1EutgiC0dT3kz5neyEwyJhn07nwEn9EnMVetBcEQcs7iy8dJfoR8A3YAjJWHdlYH0LbiA9kBXkLRcA5br31dbZPPMV6xvBZMfCLbFatKzttyh9yN9tfD3T0IoXLhUz5-J--soyWVtzbSgH_nQSaMdMQkY965lc5QnGxa4WDlkO8kn48RIzq9Wo9yoClIF22y3lHf00lOdhhkWNO0WgdvDdUyaOv8KL-Qj70cIn59qbvk8uTHn-VpcX7x82x5dF7oqq1E0fQGmBHMSECQKJmuoRe1bLThfAGGoW5ACaNQK1AcS1E1C94zVK2qsa2qXfL92TsFfz1jTN1oo8Y8lUM_x66Eki2yiW_Q_TfolZ-Dy9NlKv8aB17Df6EOPsaAfTcFO-Y3dsC6TSpdTqXbpJLRvRfhrEY0r-C_GDJQPAM3dsD1u6Lu7PL4SfgIZXqcxw</recordid><startdate>201901</startdate><enddate>201901</enddate><creator>Ren, Lei</creator><creator>Xu, Yapei</creator><creator>Qin, Guijun</creator><creator>Liu, Cong</creator><creator>Yan, Yushan</creator><creator>Zhang, Huijuan</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9504-1099</orcidid></search><sort><creationdate>201901</creationdate><title>miR‐199b‐5p‐Stonin 2 axis regulates metastases and epithelial‐to‐mesenchymal transition of papillary thyroid carcinoma</title><author>Ren, Lei ; Xu, Yapei ; Qin, Guijun ; Liu, Cong ; Yan, Yushan ; Zhang, Huijuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3839-6fd10d90da1e1aea0c41f94a6cd5571d0ec61b9dbecb1b5e293675f0eb8b4e833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adaptor Proteins, Vesicular Transport - genetics</topic><topic>Animals</topic><topic>Apoptosis</topic><topic>Cell Line, Tumor</topic><topic>Cell Movement - genetics</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - genetics</topic><topic>Chemotherapy</topic><topic>Chromosome 5</topic><topic>Ectopic expression</topic><topic>Epithelial-Mesenchymal Transition - genetics</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Heterografts</topic><topic>Humans</topic><topic>Lymphocytes B</topic><topic>Medicare</topic><topic>Mesenchyme</topic><topic>Metastases</topic><topic>metastasis</topic><topic>Mice</topic><topic>MicroRNAs - genetics</topic><topic>miRNA</topic><topic>miR‐199b‐5p</topic><topic>Paclitaxel</topic><topic>Paclitaxel - pharmacology</topic><topic>Papillary thyroid carcinoma</topic><topic>Prognosis</topic><topic>Reimbursement</topic><topic>STON2</topic><topic>Therapeutic applications</topic><topic>Thyroid</topic><topic>Thyroid cancer</topic><topic>Thyroid Cancer, Papillary - drug therapy</topic><topic>Thyroid Cancer, Papillary - genetics</topic><topic>Thyroid Cancer, Papillary - pathology</topic><topic>Tomography</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ren, Lei</creatorcontrib><creatorcontrib>Xu, Yapei</creatorcontrib><creatorcontrib>Qin, Guijun</creatorcontrib><creatorcontrib>Liu, Cong</creatorcontrib><creatorcontrib>Yan, Yushan</creatorcontrib><creatorcontrib>Zhang, Huijuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>IUBMB life</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ren, Lei</au><au>Xu, Yapei</au><au>Qin, Guijun</au><au>Liu, Cong</au><au>Yan, Yushan</au><au>Zhang, Huijuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>miR‐199b‐5p‐Stonin 2 axis regulates metastases and epithelial‐to‐mesenchymal transition of papillary thyroid carcinoma</atitle><jtitle>IUBMB life</jtitle><addtitle>IUBMB Life</addtitle><date>2019-01</date><risdate>2019</risdate><volume>71</volume><issue>1</issue><spage>28</spage><epage>40</epage><pages>28-40</pages><issn>1521-6543</issn><eissn>1521-6551</eissn><abstract>Papillary thyroid carcinoma is one of the most fatal malignant endocrine tumors, and the prognosis remains poor because of the lack of effective therapeutic targets. In this study, we demonstrated that the level of miR‐199b‐5p was markedly downregulated in papillary thyroid carcinoma. The ectopic expression level of miR‐199b‐5p in papillary thyroid carcinoma cell B‐CPAP could inhibit growth, migration, and invasion as well as epithelial‐mesenchymal transition (EMT) and decreased cell metastasis in vivo, but silencing miR‐199b‐5p caused a contradictory outcome. Additionally, Stonin 2 (STON2) was identified as a direct target gene of miR‐199b‐5p. Consistent with the downregulation of miR‐199b‐5p, the overexpression of STON2 induced the growth, migration and invasion of B‐CPAP cells. It was also demonstrated that miR‐199b‐5p suppressed papillary thyroid carcinoma cell aggressiveness by targeting STON2. Furthermore, the overexpression of miR‐199b‐5p inhibited cell proliferation, promoted apoptosis, and increased the chemo‐sensitivity of thyroid carcinoma B‐CPAP cells toward the chemotherapy drug paclitaxel. Finally, in vivo experiments further demonstrated that miR‐199b‐5p suppressed tumor growth in nude mice. Thus, this study revealed that miR‐199b‐5p functions as antioncogene miRNA in papillary thyroid carcinoma cells and that the miR‐199b‐5p/STON2 axis might be a potential treatment option for papillary thyroid carcinoma. © 2018 IUBMB Life, 71(1):28–40, 2019</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>30325582</pmid><doi>10.1002/iub.1889</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-9504-1099</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Adaptor Proteins, Vesicular Transport - genetics Animals Apoptosis Cell Line, Tumor Cell Movement - genetics Cell proliferation Cell Proliferation - genetics Chemotherapy Chromosome 5 Ectopic expression Epithelial-Mesenchymal Transition - genetics Gene Expression Regulation, Neoplastic Heterografts Humans Lymphocytes B Medicare Mesenchyme Metastases metastasis Mice MicroRNAs - genetics miRNA miR‐199b‐5p Paclitaxel Paclitaxel - pharmacology Papillary thyroid carcinoma Prognosis Reimbursement STON2 Therapeutic applications Thyroid Thyroid cancer Thyroid Cancer, Papillary - drug therapy Thyroid Cancer, Papillary - genetics Thyroid Cancer, Papillary - pathology Tomography Tumors |
| title | miR‐199b‐5p‐Stonin 2 axis regulates metastases and epithelial‐to‐mesenchymal transition of papillary thyroid carcinoma |
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