Molecular epidemiology of environmental Cryptococcus species isolates based on amplified fragment length polymorphism
Cryptococcosis is a major opportunistic fungal infection caused by members of the genus Cryptococcus, mainly those belonging to the Cryptococcus neoformans/Cryptococcus gattii species complexes. Here, we report a comprehensive molecular epidemiological study of the environmental distribution of Cryp...
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| Veröffentlicht in: | Journal de mycologie médicale 2018-12, Vol.28 (4), p.599-605 |
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| creator | Pakshir, K. Fakhim, H. Vaezi, A. Meis, J.F. Mahmoodi, M. Zomorodian, K. Javidnia, J. Ansari, S. Hagen, F. Badali, H. |
| description | Cryptococcosis is a major opportunistic fungal infection caused by members of the genus Cryptococcus, mainly those belonging to the Cryptococcus neoformans/Cryptococcus gattii species complexes. Here, we report a comprehensive molecular epidemiological study of the environmental distribution of Cryptococcus isolates in Shiraz, Iran with review of litreature.
A total of 406 samples were obtained from Eucalyptus trees and 139 samples from pigeon droppings. Cryptococcus species identification and genotyping were performed by amplified fragment length polymorphism (AFLP) fingerprinting sequencing and sequencing of the ITS rDNA region.
Majority of the isolates belonged to the Naganishia taxon (n=69) including N. albida (formerly C. albidus, n=62), N. globosa (formerly C. saitoi, n=4), N. adeliensis (formerly C. adeliensis, n=2), N. diffluens (formerly C. diffluens, n=1), and the identified C. neoformans isolates (n=25) belonged to genotype AFLP1/VNI (n=22) and AFLP1B/VNII (n=3).
More research efforts should be employed to isolate C. gattii species complex from environmental niches in Iran and provide additional evidence related to novel molecular types. |
| doi_str_mv | 10.1016/j.mycmed.2018.09.005 |
| format | Article |
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A total of 406 samples were obtained from Eucalyptus trees and 139 samples from pigeon droppings. Cryptococcus species identification and genotyping were performed by amplified fragment length polymorphism (AFLP) fingerprinting sequencing and sequencing of the ITS rDNA region.
Majority of the isolates belonged to the Naganishia taxon (n=69) including N. albida (formerly C. albidus, n=62), N. globosa (formerly C. saitoi, n=4), N. adeliensis (formerly C. adeliensis, n=2), N. diffluens (formerly C. diffluens, n=1), and the identified C. neoformans isolates (n=25) belonged to genotype AFLP1/VNI (n=22) and AFLP1B/VNII (n=3).
More research efforts should be employed to isolate C. gattii species complex from environmental niches in Iran and provide additional evidence related to novel molecular types.</description><identifier>ISSN: 1156-5233</identifier><identifier>EISSN: 1773-0449</identifier><identifier>DOI: 10.1016/j.mycmed.2018.09.005</identifier><identifier>PMID: 30322827</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Amplified Fragment Length Polymorphism (AFLP) fingerprinting ; Amplified Fragment Length Polymorphism Analysis ; Animals ; Columbidae - microbiology ; Cryptococcus - classification ; Cryptococcus - genetics ; Cryptococcus - isolation & purification ; Cryptococcus species ; DNA, Fungal - genetics ; DNA, Ribosomal Spacer - genetics ; Environmental Microbiology ; Environmental sampling ; Eucalyptus - microbiology ; Genome, Fungal - genetics ; Genotype ; Iran ; Iran - epidemiology ; Molecular Epidemiology ; Molecular Typing ; Mycological Typing Techniques ; Polymorphism, Restriction Fragment Length ; Sequence Analysis, DNA</subject><ispartof>Journal de mycologie médicale, 2018-12, Vol.28 (4), p.599-605</ispartof><rights>2018 Elsevier Masson SAS</rights><rights>Copyright © 2018 Elsevier Masson SAS. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-9b492107ea53c65c1308b3fc57da835e06f82c7da88d20a661e060337315108b3</citedby><cites>FETCH-LOGICAL-c362t-9b492107ea53c65c1308b3fc57da835e06f82c7da88d20a661e060337315108b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1156523318301641$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30322827$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pakshir, K.</creatorcontrib><creatorcontrib>Fakhim, H.</creatorcontrib><creatorcontrib>Vaezi, A.</creatorcontrib><creatorcontrib>Meis, J.F.</creatorcontrib><creatorcontrib>Mahmoodi, M.</creatorcontrib><creatorcontrib>Zomorodian, K.</creatorcontrib><creatorcontrib>Javidnia, J.</creatorcontrib><creatorcontrib>Ansari, S.</creatorcontrib><creatorcontrib>Hagen, F.</creatorcontrib><creatorcontrib>Badali, H.</creatorcontrib><title>Molecular epidemiology of environmental Cryptococcus species isolates based on amplified fragment length polymorphism</title><title>Journal de mycologie médicale</title><addtitle>J Mycol Med</addtitle><description>Cryptococcosis is a major opportunistic fungal infection caused by members of the genus Cryptococcus, mainly those belonging to the Cryptococcus neoformans/Cryptococcus gattii species complexes. Here, we report a comprehensive molecular epidemiological study of the environmental distribution of Cryptococcus isolates in Shiraz, Iran with review of litreature.
A total of 406 samples were obtained from Eucalyptus trees and 139 samples from pigeon droppings. Cryptococcus species identification and genotyping were performed by amplified fragment length polymorphism (AFLP) fingerprinting sequencing and sequencing of the ITS rDNA region.
Majority of the isolates belonged to the Naganishia taxon (n=69) including N. albida (formerly C. albidus, n=62), N. globosa (formerly C. saitoi, n=4), N. adeliensis (formerly C. adeliensis, n=2), N. diffluens (formerly C. diffluens, n=1), and the identified C. neoformans isolates (n=25) belonged to genotype AFLP1/VNI (n=22) and AFLP1B/VNII (n=3).
More research efforts should be employed to isolate C. gattii species complex from environmental niches in Iran and provide additional evidence related to novel molecular types.</description><subject>Amplified Fragment Length Polymorphism (AFLP) fingerprinting</subject><subject>Amplified Fragment Length Polymorphism Analysis</subject><subject>Animals</subject><subject>Columbidae - microbiology</subject><subject>Cryptococcus - classification</subject><subject>Cryptococcus - genetics</subject><subject>Cryptococcus - isolation & purification</subject><subject>Cryptococcus species</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Ribosomal Spacer - genetics</subject><subject>Environmental Microbiology</subject><subject>Environmental sampling</subject><subject>Eucalyptus - microbiology</subject><subject>Genome, Fungal - genetics</subject><subject>Genotype</subject><subject>Iran</subject><subject>Iran - epidemiology</subject><subject>Molecular Epidemiology</subject><subject>Molecular Typing</subject><subject>Mycological Typing Techniques</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Sequence Analysis, DNA</subject><issn>1156-5233</issn><issn>1773-0449</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOxCAUhonReH8DY1i6aeVSetmYmIm3ZIwbXROGno5MoFRoTfr20oxuXXEOfP854UPoipKcElre7nI3awdtzgitc9LkhIgDdEqrimekKJrDVFNRZoJxfoLOYtwloBSiOEYnnHDGaladounVW9CTVQHDYFpwxlu_nbHvMPTfJvjeQT8qi1dhHkavvdZTxHEAbSBiE71VYyo2KkKLfY-VG6zpTGq6oLZLFlvot-MnHrydnQ_Dp4nuAh11yka4_D3P0cfjw_vqOVu_Pb2s7teZ5iUbs2ZTNIySCpTguhSaclJveKdF1aqaCyBlVzO9NHXLiCpLmq4I5xWngi7oObrZzx2C_5ogjtKZqMFa1YOfomSUkUoUNa8TWuxRHXyMATo5BONUmCUlchEud3IvXC7CJWlk8pli178bps3y9hf6M5yAuz0A6Z_fBoKMSV2voTUB9Chbb_7f8AOjaJXF</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Pakshir, K.</creator><creator>Fakhim, H.</creator><creator>Vaezi, A.</creator><creator>Meis, J.F.</creator><creator>Mahmoodi, M.</creator><creator>Zomorodian, K.</creator><creator>Javidnia, J.</creator><creator>Ansari, S.</creator><creator>Hagen, F.</creator><creator>Badali, H.</creator><general>Elsevier Masson SAS</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201812</creationdate><title>Molecular epidemiology of environmental Cryptococcus species isolates based on amplified fragment length polymorphism</title><author>Pakshir, K. ; Fakhim, H. ; Vaezi, A. ; Meis, J.F. ; Mahmoodi, M. ; Zomorodian, K. ; Javidnia, J. ; Ansari, S. ; Hagen, F. ; Badali, H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-9b492107ea53c65c1308b3fc57da835e06f82c7da88d20a661e060337315108b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Amplified Fragment Length Polymorphism (AFLP) fingerprinting</topic><topic>Amplified Fragment Length Polymorphism Analysis</topic><topic>Animals</topic><topic>Columbidae - microbiology</topic><topic>Cryptococcus - classification</topic><topic>Cryptococcus - genetics</topic><topic>Cryptococcus - isolation & purification</topic><topic>Cryptococcus species</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Ribosomal Spacer - genetics</topic><topic>Environmental Microbiology</topic><topic>Environmental sampling</topic><topic>Eucalyptus - microbiology</topic><topic>Genome, Fungal - genetics</topic><topic>Genotype</topic><topic>Iran</topic><topic>Iran - epidemiology</topic><topic>Molecular Epidemiology</topic><topic>Molecular Typing</topic><topic>Mycological Typing Techniques</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Sequence Analysis, DNA</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pakshir, K.</creatorcontrib><creatorcontrib>Fakhim, H.</creatorcontrib><creatorcontrib>Vaezi, A.</creatorcontrib><creatorcontrib>Meis, J.F.</creatorcontrib><creatorcontrib>Mahmoodi, M.</creatorcontrib><creatorcontrib>Zomorodian, K.</creatorcontrib><creatorcontrib>Javidnia, J.</creatorcontrib><creatorcontrib>Ansari, S.</creatorcontrib><creatorcontrib>Hagen, F.</creatorcontrib><creatorcontrib>Badali, H.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal de mycologie médicale</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pakshir, K.</au><au>Fakhim, H.</au><au>Vaezi, A.</au><au>Meis, J.F.</au><au>Mahmoodi, M.</au><au>Zomorodian, K.</au><au>Javidnia, J.</au><au>Ansari, S.</au><au>Hagen, F.</au><au>Badali, H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular epidemiology of environmental Cryptococcus species isolates based on amplified fragment length polymorphism</atitle><jtitle>Journal de mycologie médicale</jtitle><addtitle>J Mycol Med</addtitle><date>2018-12</date><risdate>2018</risdate><volume>28</volume><issue>4</issue><spage>599</spage><epage>605</epage><pages>599-605</pages><issn>1156-5233</issn><eissn>1773-0449</eissn><abstract>Cryptococcosis is a major opportunistic fungal infection caused by members of the genus Cryptococcus, mainly those belonging to the Cryptococcus neoformans/Cryptococcus gattii species complexes. Here, we report a comprehensive molecular epidemiological study of the environmental distribution of Cryptococcus isolates in Shiraz, Iran with review of litreature.
A total of 406 samples were obtained from Eucalyptus trees and 139 samples from pigeon droppings. Cryptococcus species identification and genotyping were performed by amplified fragment length polymorphism (AFLP) fingerprinting sequencing and sequencing of the ITS rDNA region.
Majority of the isolates belonged to the Naganishia taxon (n=69) including N. albida (formerly C. albidus, n=62), N. globosa (formerly C. saitoi, n=4), N. adeliensis (formerly C. adeliensis, n=2), N. diffluens (formerly C. diffluens, n=1), and the identified C. neoformans isolates (n=25) belonged to genotype AFLP1/VNI (n=22) and AFLP1B/VNII (n=3).
More research efforts should be employed to isolate C. gattii species complex from environmental niches in Iran and provide additional evidence related to novel molecular types.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>30322827</pmid><doi>10.1016/j.mycmed.2018.09.005</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal de mycologie médicale, 2018-12, Vol.28 (4), p.599-605 |
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| subjects | Amplified Fragment Length Polymorphism (AFLP) fingerprinting Amplified Fragment Length Polymorphism Analysis Animals Columbidae - microbiology Cryptococcus - classification Cryptococcus - genetics Cryptococcus - isolation & purification Cryptococcus species DNA, Fungal - genetics DNA, Ribosomal Spacer - genetics Environmental Microbiology Environmental sampling Eucalyptus - microbiology Genome, Fungal - genetics Genotype Iran Iran - epidemiology Molecular Epidemiology Molecular Typing Mycological Typing Techniques Polymorphism, Restriction Fragment Length Sequence Analysis, DNA |
| title | Molecular epidemiology of environmental Cryptococcus species isolates based on amplified fragment length polymorphism |
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