Fractal dimension in the evaluation of different treatments of muscular injury in rats
•The Fractal Dimension can be used to identify the process of inflammation in the skeletal muscle.•Low level laser therapy and platelet rich plasma can enhance the cicatrization process when used separately or in combination.•The box-counting method of Fractal Dimension is not able to differentiate...
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| Veröffentlicht in: | Tissue & cell 2018-10, Vol.54, p.120-126 |
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| creator | Garcia, Thiago Alves Tamura Ozaki, Guilherme Akio Castoldi, Robson Chacon Koike, Tatiana Emy Trindade Camargo, Regina Celi Silva Camargo Filho, José Carlos |
| description | •The Fractal Dimension can be used to identify the process of inflammation in the skeletal muscle.•Low level laser therapy and platelet rich plasma can enhance the cicatrization process when used separately or in combination.•The box-counting method of Fractal Dimension is not able to differentiate muscle injury in different healing treatments.
To evaluate alterations from different therapies in muscular injury using the Fractal Dimension (FD) method.
35 animals were allocated in Control Group (C), Injury Control Group (IC), Injury Low Level Laser Therapy Group (ILT), Injury Platelet Rich Plasma Group (IP), and Injury LLLT and PRP Group (ILP). The animals suffered a stretch injury in gastrocnemius muscle and after that IP and ILP groups received PRP application. The ILT and ILP groups received daily LLLT applications for seven days. After seven days the animals were euthanized and the gastrocnemius muscle removed and frozen. The muscles were stained with Hematoxylin and Eosin (HE) and Picrosirius Red, for observation of the morphology of the injury and semi-quantitative and quantitative analysis through the Fractal Dimension (FD) method.
In the qualitative and semi-quantitative analysis, in relation to IC group, the ILT presented a reduction in rounded fibers and the IP in angular fibers. The ILP group demonstrated a reduction in both polymorphic fibers and inflammatory infiltrate. The FD of the muscles stained with HE was higher in the groups that suffered the injury when compared to the C group (p |
| doi_str_mv | 10.1016/j.tice.2018.08.014 |
| format | Article |
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To evaluate alterations from different therapies in muscular injury using the Fractal Dimension (FD) method.
35 animals were allocated in Control Group (C), Injury Control Group (IC), Injury Low Level Laser Therapy Group (ILT), Injury Platelet Rich Plasma Group (IP), and Injury LLLT and PRP Group (ILP). The animals suffered a stretch injury in gastrocnemius muscle and after that IP and ILP groups received PRP application. The ILT and ILP groups received daily LLLT applications for seven days. After seven days the animals were euthanized and the gastrocnemius muscle removed and frozen. The muscles were stained with Hematoxylin and Eosin (HE) and Picrosirius Red, for observation of the morphology of the injury and semi-quantitative and quantitative analysis through the Fractal Dimension (FD) method.
In the qualitative and semi-quantitative analysis, in relation to IC group, the ILT presented a reduction in rounded fibers and the IP in angular fibers. The ILP group demonstrated a reduction in both polymorphic fibers and inflammatory infiltrate. The FD of the muscles stained with HE was higher in the groups that suffered the injury when compared to the C group (p < 0.05); the FD of the collagen demonstrated no statistical difference between the groups.
Both treatments were able to accelerate injury repair, and the association of both presented better results than the isolated applications. However, the FD method showed no sensitivity to differentiate the treatments, either in the histological aspects or the injury in collagen.</description><identifier>ISSN: 0040-8166</identifier><identifier>EISSN: 1532-3072</identifier><identifier>DOI: 10.1016/j.tice.2018.08.014</identifier><identifier>PMID: 30309501</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Animals ; Collagen ; Fibers ; Fractal analysis ; Fractal dimension ; Fractals ; Gastrocnemius muscle ; Histology ; Inflammation ; Injuries ; Injury analysis ; Low level ; Low level laser therapy ; Low-Level Light Therapy - methods ; Male ; Morphology ; Muscle, Skeletal - injuries ; Muscle, Skeletal - radiation effects ; Muscle, Skeletal - surgery ; Muscles ; Muscular system ; Platelet rich plasma ; Qualitative analysis ; Quantitative analysis ; Rats ; Rats, Wistar ; Reduction ; Treatment Outcome</subject><ispartof>Tissue & cell, 2018-10, Vol.54, p.120-126</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Oct 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-e27436a5110a6e20573dd8d0399454900ece25b7db47859247c6945246f632e93</citedby><cites>FETCH-LOGICAL-c428t-e27436a5110a6e20573dd8d0399454900ece25b7db47859247c6945246f632e93</cites><orcidid>0000-0003-1031-038X ; 0000-0002-9423-9965</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0040816617304275$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30309501$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Garcia, Thiago Alves</creatorcontrib><creatorcontrib>Tamura Ozaki, Guilherme Akio</creatorcontrib><creatorcontrib>Castoldi, Robson Chacon</creatorcontrib><creatorcontrib>Koike, Tatiana Emy</creatorcontrib><creatorcontrib>Trindade Camargo, Regina Celi</creatorcontrib><creatorcontrib>Silva Camargo Filho, José Carlos</creatorcontrib><title>Fractal dimension in the evaluation of different treatments of muscular injury in rats</title><title>Tissue & cell</title><addtitle>Tissue Cell</addtitle><description>•The Fractal Dimension can be used to identify the process of inflammation in the skeletal muscle.•Low level laser therapy and platelet rich plasma can enhance the cicatrization process when used separately or in combination.•The box-counting method of Fractal Dimension is not able to differentiate muscle injury in different healing treatments.
To evaluate alterations from different therapies in muscular injury using the Fractal Dimension (FD) method.
35 animals were allocated in Control Group (C), Injury Control Group (IC), Injury Low Level Laser Therapy Group (ILT), Injury Platelet Rich Plasma Group (IP), and Injury LLLT and PRP Group (ILP). The animals suffered a stretch injury in gastrocnemius muscle and after that IP and ILP groups received PRP application. The ILT and ILP groups received daily LLLT applications for seven days. After seven days the animals were euthanized and the gastrocnemius muscle removed and frozen. The muscles were stained with Hematoxylin and Eosin (HE) and Picrosirius Red, for observation of the morphology of the injury and semi-quantitative and quantitative analysis through the Fractal Dimension (FD) method.
In the qualitative and semi-quantitative analysis, in relation to IC group, the ILT presented a reduction in rounded fibers and the IP in angular fibers. The ILP group demonstrated a reduction in both polymorphic fibers and inflammatory infiltrate. The FD of the muscles stained with HE was higher in the groups that suffered the injury when compared to the C group (p < 0.05); the FD of the collagen demonstrated no statistical difference between the groups.
Both treatments were able to accelerate injury repair, and the association of both presented better results than the isolated applications. However, the FD method showed no sensitivity to differentiate the treatments, either in the histological aspects or the injury in collagen.</description><subject>Animals</subject><subject>Collagen</subject><subject>Fibers</subject><subject>Fractal analysis</subject><subject>Fractal dimension</subject><subject>Fractals</subject><subject>Gastrocnemius muscle</subject><subject>Histology</subject><subject>Inflammation</subject><subject>Injuries</subject><subject>Injury analysis</subject><subject>Low level</subject><subject>Low level laser therapy</subject><subject>Low-Level Light Therapy - methods</subject><subject>Male</subject><subject>Morphology</subject><subject>Muscle, Skeletal - injuries</subject><subject>Muscle, Skeletal - radiation effects</subject><subject>Muscle, Skeletal - surgery</subject><subject>Muscles</subject><subject>Muscular system</subject><subject>Platelet rich plasma</subject><subject>Qualitative analysis</subject><subject>Quantitative analysis</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reduction</subject><subject>Treatment Outcome</subject><issn>0040-8166</issn><issn>1532-3072</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKxDAUhoMoznh5ARdScOOm48mlTQNuZPAGght1GzLpKab0okkq-PamjLpwIRwInPP9P-Ej5ITCigItL9pVdBZXDGi1gjRU7JAlLTjLOUi2S5YAAvKKluWCHITQAoAUVO6TBQcOqgC6JC833thouqx2PQ7BjUPmhiy-YoYfpptMnDdjk85Ngx6HmEWPJiY2hnnfT8FOnfEp1U7-cw57E8MR2WtMF_D4-z0kzzfXT-u7_OHx9n599ZBbwaqYI5OCl6agFEyJDArJ67qqgSslCqEA0CIrNrLeCFkViglpy3RhomxKzlDxQ3K-7X3z4_uEIereBYtdZwYcp6AZpUoxpiQk9OwP2o6TH9LvEsWBVVRUVaLYlrJ-DMFjo9-8643_1BT0bF23erauZ-sa0lCRQqff1dOmx_o38qM5AZdbAJOLD4deB-twsFg7jzbqenT_9X8B5-SRZw</recordid><startdate>201810</startdate><enddate>201810</enddate><creator>Garcia, Thiago Alves</creator><creator>Tamura Ozaki, Guilherme Akio</creator><creator>Castoldi, Robson Chacon</creator><creator>Koike, Tatiana Emy</creator><creator>Trindade Camargo, Regina Celi</creator><creator>Silva Camargo Filho, José Carlos</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1031-038X</orcidid><orcidid>https://orcid.org/0000-0002-9423-9965</orcidid></search><sort><creationdate>201810</creationdate><title>Fractal dimension in the evaluation of different treatments of muscular injury in rats</title><author>Garcia, Thiago Alves ; Tamura Ozaki, Guilherme Akio ; Castoldi, Robson Chacon ; Koike, Tatiana Emy ; Trindade Camargo, Regina Celi ; Silva Camargo Filho, José Carlos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-e27436a5110a6e20573dd8d0399454900ece25b7db47859247c6945246f632e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Collagen</topic><topic>Fibers</topic><topic>Fractal analysis</topic><topic>Fractal dimension</topic><topic>Fractals</topic><topic>Gastrocnemius muscle</topic><topic>Histology</topic><topic>Inflammation</topic><topic>Injuries</topic><topic>Injury analysis</topic><topic>Low level</topic><topic>Low level laser therapy</topic><topic>Low-Level Light Therapy - methods</topic><topic>Male</topic><topic>Morphology</topic><topic>Muscle, Skeletal - injuries</topic><topic>Muscle, Skeletal - radiation effects</topic><topic>Muscle, Skeletal - surgery</topic><topic>Muscles</topic><topic>Muscular system</topic><topic>Platelet rich plasma</topic><topic>Qualitative analysis</topic><topic>Quantitative analysis</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reduction</topic><topic>Treatment Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Garcia, Thiago Alves</creatorcontrib><creatorcontrib>Tamura Ozaki, Guilherme Akio</creatorcontrib><creatorcontrib>Castoldi, Robson Chacon</creatorcontrib><creatorcontrib>Koike, Tatiana Emy</creatorcontrib><creatorcontrib>Trindade Camargo, Regina Celi</creatorcontrib><creatorcontrib>Silva Camargo Filho, José Carlos</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Tissue & cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Garcia, Thiago Alves</au><au>Tamura Ozaki, Guilherme Akio</au><au>Castoldi, Robson Chacon</au><au>Koike, Tatiana Emy</au><au>Trindade Camargo, Regina Celi</au><au>Silva Camargo Filho, José Carlos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fractal dimension in the evaluation of different treatments of muscular injury in rats</atitle><jtitle>Tissue & cell</jtitle><addtitle>Tissue Cell</addtitle><date>2018-10</date><risdate>2018</risdate><volume>54</volume><spage>120</spage><epage>126</epage><pages>120-126</pages><issn>0040-8166</issn><eissn>1532-3072</eissn><abstract>•The Fractal Dimension can be used to identify the process of inflammation in the skeletal muscle.•Low level laser therapy and platelet rich plasma can enhance the cicatrization process when used separately or in combination.•The box-counting method of Fractal Dimension is not able to differentiate muscle injury in different healing treatments.
To evaluate alterations from different therapies in muscular injury using the Fractal Dimension (FD) method.
35 animals were allocated in Control Group (C), Injury Control Group (IC), Injury Low Level Laser Therapy Group (ILT), Injury Platelet Rich Plasma Group (IP), and Injury LLLT and PRP Group (ILP). The animals suffered a stretch injury in gastrocnemius muscle and after that IP and ILP groups received PRP application. The ILT and ILP groups received daily LLLT applications for seven days. After seven days the animals were euthanized and the gastrocnemius muscle removed and frozen. The muscles were stained with Hematoxylin and Eosin (HE) and Picrosirius Red, for observation of the morphology of the injury and semi-quantitative and quantitative analysis through the Fractal Dimension (FD) method.
In the qualitative and semi-quantitative analysis, in relation to IC group, the ILT presented a reduction in rounded fibers and the IP in angular fibers. The ILP group demonstrated a reduction in both polymorphic fibers and inflammatory infiltrate. The FD of the muscles stained with HE was higher in the groups that suffered the injury when compared to the C group (p < 0.05); the FD of the collagen demonstrated no statistical difference between the groups.
Both treatments were able to accelerate injury repair, and the association of both presented better results than the isolated applications. However, the FD method showed no sensitivity to differentiate the treatments, either in the histological aspects or the injury in collagen.</abstract><cop>Scotland</cop><pub>Elsevier Ltd</pub><pmid>30309501</pmid><doi>10.1016/j.tice.2018.08.014</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-1031-038X</orcidid><orcidid>https://orcid.org/0000-0002-9423-9965</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Collagen Fibers Fractal analysis Fractal dimension Fractals Gastrocnemius muscle Histology Inflammation Injuries Injury analysis Low level Low level laser therapy Low-Level Light Therapy - methods Male Morphology Muscle, Skeletal - injuries Muscle, Skeletal - radiation effects Muscle, Skeletal - surgery Muscles Muscular system Platelet rich plasma Qualitative analysis Quantitative analysis Rats Rats, Wistar Reduction Treatment Outcome |
| title | Fractal dimension in the evaluation of different treatments of muscular injury in rats |
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