Staying alive: growth and survival of Bifidobacterium animalis subsp. animalis under in vitro and in vivo conditions
Members of the Bifidobacterium genus are widely used as probiotics in fermented milk products. Bifidobacterium animalis subsp. animalis CNCM I-4602 grows and survives poorly in reconstituted skimmed milk (RSM). Availing of genome and transcriptome information, this poor growth and survival phenotype...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2018-12, Vol.102 (24), p.10645-10663 |
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| creator | Egan, Muireann Bottacini, Francesca O’Connell Motherway, Mary Casey, Patrick G. Morrissey, Ruth Melgar, Silvia Faurie, Jean-Michel Chervaux, Christian Smokvina, Tamara van Sinderen, Douwe |
| description | Members of the
Bifidobacterium
genus are widely used as probiotics in fermented milk products.
Bifidobacterium animalis
subsp.
animalis
CNCM I-4602 grows and survives poorly in reconstituted skimmed milk (RSM). Availing of genome and transcriptome information, this poor growth and survival phenotype in milk was substantially improved by the addition of certain compounds, such as yeast extract, uric acid, glutathione, cysteine, ferrous sulfate, and a combination of magnesium sulfate and manganese sulfate. Carbohydrate utilization of CNCM I-4602 was also investigated, allowing the identification of several carbohydrate utilization gene clusters, and highlighting this strain’s inability to utilize lactose, unlike the type strain of this subspecies,
B. animalis
subsp.
animalis
ATCC25527 and the
B. animalis
subsp.
lactis
subspecies. In addition, the ability of
B. animalis
subsp.
animalis
CNCM I-4602 to colonize a murine model was investigated, which showed that this strain persists in the murine gut for a period of at least 4 weeks. Associated in vivo transcriptome analysis revealed that, among other genes, a gene cluster encoding a predicted type IVb tight adherence (Tad) pilus was upregulated, indicating that this extracellular structure plays a role in the colonization/adaptation of the murine gastrointestinal tract by this strain. |
| doi_str_mv | 10.1007/s00253-018-9413-7 |
| format | Article |
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Bifidobacterium
genus are widely used as probiotics in fermented milk products.
Bifidobacterium animalis
subsp.
animalis
CNCM I-4602 grows and survives poorly in reconstituted skimmed milk (RSM). Availing of genome and transcriptome information, this poor growth and survival phenotype in milk was substantially improved by the addition of certain compounds, such as yeast extract, uric acid, glutathione, cysteine, ferrous sulfate, and a combination of magnesium sulfate and manganese sulfate. Carbohydrate utilization of CNCM I-4602 was also investigated, allowing the identification of several carbohydrate utilization gene clusters, and highlighting this strain’s inability to utilize lactose, unlike the type strain of this subspecies,
B. animalis
subsp.
animalis
ATCC25527 and the
B. animalis
subsp.
lactis
subspecies. In addition, the ability of
B. animalis
subsp.
animalis
CNCM I-4602 to colonize a murine model was investigated, which showed that this strain persists in the murine gut for a period of at least 4 weeks. Associated in vivo transcriptome analysis revealed that, among other genes, a gene cluster encoding a predicted type IVb tight adherence (Tad) pilus was upregulated, indicating that this extracellular structure plays a role in the colonization/adaptation of the murine gastrointestinal tract by this strain.</description><identifier>ISSN: 0175-7598</identifier><identifier>EISSN: 1432-0614</identifier><identifier>DOI: 10.1007/s00253-018-9413-7</identifier><identifier>PMID: 30306201</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Animal models ; Animals ; Bifidobacteria ; Bifidobacterium animalis ; Bifidobacterium animalis - drug effects ; Bifidobacterium animalis - genetics ; Bifidobacterium animalis - growth & development ; Biomedical and Life Sciences ; Biotechnology ; Carbohydrate Metabolism ; Carbohydrates ; Colonization ; Drug Resistance, Microbial ; Female ; Fermented milk products ; Ferrous sulfate ; Food Microbiology - methods ; Gastrointestinal Microbiome ; Gastrointestinal system ; Gastrointestinal tract ; Gene clusters ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Genetic aspects ; Genome, Bacterial ; Genomes ; Genomics ; Glutathione ; Growth ; Iron sulfates ; Lactose ; Life Sciences ; Magnesium sulfate ; Manganese ; Manganese sulfate ; Mice, Inbred BALB C ; Microbial Genetics and Genomics ; Microbiology ; Milk ; Milk - microbiology ; Milk products ; Phenotypes ; Physiological aspects ; Probiotics ; Proteomics ; Sulfates ; Survival ; Transcriptomics ; Uric acid ; Yeast ; Yeasts</subject><ispartof>Applied microbiology and biotechnology, 2018-12, Vol.102 (24), p.10645-10663</ispartof><rights>Springer-Verlag GmbH Germany, part of Springer Nature 2018</rights><rights>COPYRIGHT 2018 Springer</rights><rights>Applied Microbiology and Biotechnology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c510t-6b2d37f0a1b9f0517a3a2dd496e1414c93a98fab9e6c644f407f47cb2e81185f3</citedby><cites>FETCH-LOGICAL-c510t-6b2d37f0a1b9f0517a3a2dd496e1414c93a98fab9e6c644f407f47cb2e81185f3</cites><orcidid>0000-0002-0142-2956</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-018-9413-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-018-9413-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30306201$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Egan, Muireann</creatorcontrib><creatorcontrib>Bottacini, Francesca</creatorcontrib><creatorcontrib>O’Connell Motherway, Mary</creatorcontrib><creatorcontrib>Casey, Patrick G.</creatorcontrib><creatorcontrib>Morrissey, Ruth</creatorcontrib><creatorcontrib>Melgar, Silvia</creatorcontrib><creatorcontrib>Faurie, Jean-Michel</creatorcontrib><creatorcontrib>Chervaux, Christian</creatorcontrib><creatorcontrib>Smokvina, Tamara</creatorcontrib><creatorcontrib>van Sinderen, Douwe</creatorcontrib><title>Staying alive: growth and survival of Bifidobacterium animalis subsp. animalis under in vitro and in vivo conditions</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>Members of the
Bifidobacterium
genus are widely used as probiotics in fermented milk products.
Bifidobacterium animalis
subsp.
animalis
CNCM I-4602 grows and survives poorly in reconstituted skimmed milk (RSM). Availing of genome and transcriptome information, this poor growth and survival phenotype in milk was substantially improved by the addition of certain compounds, such as yeast extract, uric acid, glutathione, cysteine, ferrous sulfate, and a combination of magnesium sulfate and manganese sulfate. Carbohydrate utilization of CNCM I-4602 was also investigated, allowing the identification of several carbohydrate utilization gene clusters, and highlighting this strain’s inability to utilize lactose, unlike the type strain of this subspecies,
B. animalis
subsp.
animalis
ATCC25527 and the
B. animalis
subsp.
lactis
subspecies. In addition, the ability of
B. animalis
subsp.
animalis
CNCM I-4602 to colonize a murine model was investigated, which showed that this strain persists in the murine gut for a period of at least 4 weeks. Associated in vivo transcriptome analysis revealed that, among other genes, a gene cluster encoding a predicted type IVb tight adherence (Tad) pilus was upregulated, indicating that this extracellular structure plays a role in the colonization/adaptation of the murine gastrointestinal tract by this strain.</description><subject>Animal models</subject><subject>Animals</subject><subject>Bifidobacteria</subject><subject>Bifidobacterium animalis</subject><subject>Bifidobacterium animalis - drug effects</subject><subject>Bifidobacterium animalis - genetics</subject><subject>Bifidobacterium animalis - growth & development</subject><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Carbohydrate Metabolism</subject><subject>Carbohydrates</subject><subject>Colonization</subject><subject>Drug Resistance, Microbial</subject><subject>Female</subject><subject>Fermented milk products</subject><subject>Ferrous sulfate</subject><subject>Food Microbiology - methods</subject><subject>Gastrointestinal Microbiome</subject><subject>Gastrointestinal system</subject><subject>Gastrointestinal tract</subject><subject>Gene clusters</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genetic aspects</subject><subject>Genome, Bacterial</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Glutathione</subject><subject>Growth</subject><subject>Iron sulfates</subject><subject>Lactose</subject><subject>Life Sciences</subject><subject>Magnesium sulfate</subject><subject>Manganese</subject><subject>Manganese sulfate</subject><subject>Mice, Inbred BALB C</subject><subject>Microbial Genetics and Genomics</subject><subject>Microbiology</subject><subject>Milk</subject><subject>Milk - microbiology</subject><subject>Milk products</subject><subject>Phenotypes</subject><subject>Physiological aspects</subject><subject>Probiotics</subject><subject>Proteomics</subject><subject>Sulfates</subject><subject>Survival</subject><subject>Transcriptomics</subject><subject>Uric acid</subject><subject>Yeast</subject><subject>Yeasts</subject><issn>0175-7598</issn><issn>1432-0614</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kk9v1DAQxS0EokvhA3BBkbjAIcs4duyEW1vxp1IlJApny3Hs4CqxF9sJ9NvjdAurRSAfrPH83pNn9BB6jmGLAfibCFDVpATclC3FpOQP0AZTUpXAMH2INoB5XfK6bU7QkxhvAHDVMPYYnRAgwCrAG5Suk7y1bijkaBf9thiC_5G-FdL1RZzDYhc5Ft4U59bY3ndSJR3sPOW-nbIiZqiLu-2hnl2vQ2FdsdgU_J3PXbH4QnnX22S9i0_RIyPHqJ_d36fo6_t3Xy4-llefPlxenF2VqsaQStZVPeEGJO5aAzXmksiq72nLNKaYqpbItjGyazVTjFJDgRvKVVfpBuOmNuQUvdr77oL_PuuYxGSj0uMonfZzFFXGCCaUkYy-_Au98XNw-XcrxVn25s2BGuSohXXGpyDVairOasYYAK1XavsPKp9eTzZvQRub348Er48EmUn6ZxrkHKO4vP58zOI9q4KPMWgjdiHvPtwKDGJNhdinQuRUiDUVgmfNi_vh5m7S_R_F7xhkoNoDMbfcoMNh-v-7_gLjkb_9</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Egan, Muireann</creator><creator>Bottacini, Francesca</creator><creator>O’Connell Motherway, Mary</creator><creator>Casey, Patrick G.</creator><creator>Morrissey, Ruth</creator><creator>Melgar, Silvia</creator><creator>Faurie, Jean-Michel</creator><creator>Chervaux, Christian</creator><creator>Smokvina, Tamara</creator><creator>van Sinderen, Douwe</creator><general>Springer Berlin Heidelberg</general><general>Springer</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0142-2956</orcidid></search><sort><creationdate>20181201</creationdate><title>Staying alive: growth and survival of Bifidobacterium animalis subsp. animalis under in vitro and in vivo conditions</title><author>Egan, Muireann ; Bottacini, Francesca ; O’Connell Motherway, Mary ; Casey, Patrick G. ; Morrissey, Ruth ; Melgar, Silvia ; Faurie, Jean-Michel ; Chervaux, Christian ; Smokvina, Tamara ; van Sinderen, Douwe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c510t-6b2d37f0a1b9f0517a3a2dd496e1414c93a98fab9e6c644f407f47cb2e81185f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal models</topic><topic>Animals</topic><topic>Bifidobacteria</topic><topic>Bifidobacterium animalis</topic><topic>Bifidobacterium animalis - drug effects</topic><topic>Bifidobacterium animalis - genetics</topic><topic>Bifidobacterium animalis - growth & development</topic><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Carbohydrate Metabolism</topic><topic>Carbohydrates</topic><topic>Colonization</topic><topic>Drug Resistance, Microbial</topic><topic>Female</topic><topic>Fermented milk products</topic><topic>Ferrous sulfate</topic><topic>Food Microbiology - methods</topic><topic>Gastrointestinal Microbiome</topic><topic>Gastrointestinal system</topic><topic>Gastrointestinal tract</topic><topic>Gene clusters</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genetic aspects</topic><topic>Genome, Bacterial</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Glutathione</topic><topic>Growth</topic><topic>Iron sulfates</topic><topic>Lactose</topic><topic>Life Sciences</topic><topic>Magnesium sulfate</topic><topic>Manganese</topic><topic>Manganese sulfate</topic><topic>Mice, Inbred BALB C</topic><topic>Microbial Genetics and Genomics</topic><topic>Microbiology</topic><topic>Milk</topic><topic>Milk - microbiology</topic><topic>Milk products</topic><topic>Phenotypes</topic><topic>Physiological aspects</topic><topic>Probiotics</topic><topic>Proteomics</topic><topic>Sulfates</topic><topic>Survival</topic><topic>Transcriptomics</topic><topic>Uric acid</topic><topic>Yeast</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Egan, Muireann</creatorcontrib><creatorcontrib>Bottacini, Francesca</creatorcontrib><creatorcontrib>O’Connell Motherway, Mary</creatorcontrib><creatorcontrib>Casey, Patrick G.</creatorcontrib><creatorcontrib>Morrissey, Ruth</creatorcontrib><creatorcontrib>Melgar, Silvia</creatorcontrib><creatorcontrib>Faurie, Jean-Michel</creatorcontrib><creatorcontrib>Chervaux, Christian</creatorcontrib><creatorcontrib>Smokvina, Tamara</creatorcontrib><creatorcontrib>van Sinderen, Douwe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>ABI/INFORM Collection</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Applied microbiology and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Egan, Muireann</au><au>Bottacini, Francesca</au><au>O’Connell Motherway, Mary</au><au>Casey, Patrick G.</au><au>Morrissey, Ruth</au><au>Melgar, Silvia</au><au>Faurie, Jean-Michel</au><au>Chervaux, Christian</au><au>Smokvina, Tamara</au><au>van Sinderen, Douwe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Staying alive: growth and survival of Bifidobacterium animalis subsp. animalis under in vitro and in vivo conditions</atitle><jtitle>Applied microbiology and biotechnology</jtitle><stitle>Appl Microbiol Biotechnol</stitle><addtitle>Appl Microbiol Biotechnol</addtitle><date>2018-12-01</date><risdate>2018</risdate><volume>102</volume><issue>24</issue><spage>10645</spage><epage>10663</epage><pages>10645-10663</pages><issn>0175-7598</issn><eissn>1432-0614</eissn><abstract>Members of the
Bifidobacterium
genus are widely used as probiotics in fermented milk products.
Bifidobacterium animalis
subsp.
animalis
CNCM I-4602 grows and survives poorly in reconstituted skimmed milk (RSM). Availing of genome and transcriptome information, this poor growth and survival phenotype in milk was substantially improved by the addition of certain compounds, such as yeast extract, uric acid, glutathione, cysteine, ferrous sulfate, and a combination of magnesium sulfate and manganese sulfate. Carbohydrate utilization of CNCM I-4602 was also investigated, allowing the identification of several carbohydrate utilization gene clusters, and highlighting this strain’s inability to utilize lactose, unlike the type strain of this subspecies,
B. animalis
subsp.
animalis
ATCC25527 and the
B. animalis
subsp.
lactis
subspecies. In addition, the ability of
B. animalis
subsp.
animalis
CNCM I-4602 to colonize a murine model was investigated, which showed that this strain persists in the murine gut for a period of at least 4 weeks. Associated in vivo transcriptome analysis revealed that, among other genes, a gene cluster encoding a predicted type IVb tight adherence (Tad) pilus was upregulated, indicating that this extracellular structure plays a role in the colonization/adaptation of the murine gastrointestinal tract by this strain.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><pmid>30306201</pmid><doi>10.1007/s00253-018-9413-7</doi><tpages>19</tpages><orcidid>https://orcid.org/0000-0002-0142-2956</orcidid></addata></record> |
| fulltext | fulltext |
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| ispartof | Applied microbiology and biotechnology, 2018-12, Vol.102 (24), p.10645-10663 |
| issn | 0175-7598 1432-0614 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2118313463 |
| source | MEDLINE; Springer Nature - Complete Springer Journals |
| subjects | Animal models Animals Bifidobacteria Bifidobacterium animalis Bifidobacterium animalis - drug effects Bifidobacterium animalis - genetics Bifidobacterium animalis - growth & development Biomedical and Life Sciences Biotechnology Carbohydrate Metabolism Carbohydrates Colonization Drug Resistance, Microbial Female Fermented milk products Ferrous sulfate Food Microbiology - methods Gastrointestinal Microbiome Gastrointestinal system Gastrointestinal tract Gene clusters Gene Expression Profiling Gene Expression Regulation, Bacterial Genetic aspects Genome, Bacterial Genomes Genomics Glutathione Growth Iron sulfates Lactose Life Sciences Magnesium sulfate Manganese Manganese sulfate Mice, Inbred BALB C Microbial Genetics and Genomics Microbiology Milk Milk - microbiology Milk products Phenotypes Physiological aspects Probiotics Proteomics Sulfates Survival Transcriptomics Uric acid Yeast Yeasts |
| title | Staying alive: growth and survival of Bifidobacterium animalis subsp. animalis under in vitro and in vivo conditions |
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