Biological studies of synthesized silver nanoparticles using Prosopis farcta
The evaluation of cytotoxic and apoptotic activities of silver nanoparticles (Ag-NPs) synthesized by aqueous extract of Prosopis farcta was investigated against lung (A549) and colon (HT-29) cell lines. The cytotoxic activity of nanoparticles was performed using MTT assay, while their apoptotic acti...
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| Veröffentlicht in: | Molecular biology reports 2018-12, Vol.45 (6), p.1621-1626 |
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| creator | Miri, Abdolhossien Sarani, Mina |
| description | The evaluation of cytotoxic and apoptotic activities of silver nanoparticles (Ag-NPs) synthesized by aqueous extract of
Prosopis farcta
was investigated against lung (A549) and colon (HT-29) cell lines. The cytotoxic activity of nanoparticles was performed using MTT assay, while their apoptotic activity was tested using TUNEL method. The obtained results of MTT showed that the cell viability of A549 was dependent on the nanoparticles concentration and incubation time. Therefore, although the cytotoxic effect increased as the Ag-NPs concentration and incubation time heightened, yet the viability of HT-29 cells seems to be dependent only on the incubation time. The apoptotic results of the nanoparticles showed more than 50% of apoptosis on A549 and HT-29 cell lines, which in this case, HT-29 demonstrated 100% apoptosis at concentrations of more than 400 µg/ml. It seems that Ag-NPs synthesized using
P. farcta
extract can serve as anti-cancer agent in the treatment many cancers through creating or discovering new drug forms. |
| doi_str_mv | 10.1007/s11033-018-4299-0 |
| format | Article |
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Prosopis farcta
was investigated against lung (A549) and colon (HT-29) cell lines. The cytotoxic activity of nanoparticles was performed using MTT assay, while their apoptotic activity was tested using TUNEL method. The obtained results of MTT showed that the cell viability of A549 was dependent on the nanoparticles concentration and incubation time. Therefore, although the cytotoxic effect increased as the Ag-NPs concentration and incubation time heightened, yet the viability of HT-29 cells seems to be dependent only on the incubation time. The apoptotic results of the nanoparticles showed more than 50% of apoptosis on A549 and HT-29 cell lines, which in this case, HT-29 demonstrated 100% apoptosis at concentrations of more than 400 µg/ml. It seems that Ag-NPs synthesized using
P. farcta
extract can serve as anti-cancer agent in the treatment many cancers through creating or discovering new drug forms.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-018-4299-0</identifier><identifier>PMID: 30293192</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>A549 Cells - drug effects ; Animal Anatomy ; Animal Biochemistry ; Apoptosis ; Apoptosis - drug effects ; Biomedical and Life Sciences ; Cancer ; Cell Survival - drug effects ; Colon ; Colorectal cancer ; Cytotoxicity ; Histology ; HT29 Cells - drug effects ; Humans ; Life Sciences ; Lung cancer ; Metal Nanoparticles - adverse effects ; Morphology ; Nanoparticles ; Original Article ; Plant Extracts - pharmacology ; Prosopis - adverse effects ; Prosopis farcta ; Silver ; Silver - adverse effects ; Silver - metabolism ; Spectrophotometry, Ultraviolet ; X-Ray Diffraction</subject><ispartof>Molecular biology reports, 2018-12, Vol.45 (6), p.1621-1626</ispartof><rights>Springer Nature B.V. 2018</rights><rights>Molecular Biology Reports is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-51d2c0b710d532d885ba43426d0fd2f654690b74d98af14b1bdc992d4051a4a23</citedby><cites>FETCH-LOGICAL-c372t-51d2c0b710d532d885ba43426d0fd2f654690b74d98af14b1bdc992d4051a4a23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-018-4299-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-018-4299-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30293192$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Miri, Abdolhossien</creatorcontrib><creatorcontrib>Sarani, Mina</creatorcontrib><title>Biological studies of synthesized silver nanoparticles using Prosopis farcta</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>The evaluation of cytotoxic and apoptotic activities of silver nanoparticles (Ag-NPs) synthesized by aqueous extract of
Prosopis farcta
was investigated against lung (A549) and colon (HT-29) cell lines. The cytotoxic activity of nanoparticles was performed using MTT assay, while their apoptotic activity was tested using TUNEL method. The obtained results of MTT showed that the cell viability of A549 was dependent on the nanoparticles concentration and incubation time. Therefore, although the cytotoxic effect increased as the Ag-NPs concentration and incubation time heightened, yet the viability of HT-29 cells seems to be dependent only on the incubation time. The apoptotic results of the nanoparticles showed more than 50% of apoptosis on A549 and HT-29 cell lines, which in this case, HT-29 demonstrated 100% apoptosis at concentrations of more than 400 µg/ml. It seems that Ag-NPs synthesized using
P. farcta
extract can serve as anti-cancer agent in the treatment many cancers through creating or discovering new drug forms.</description><subject>A549 Cells - drug effects</subject><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biomedical and Life Sciences</subject><subject>Cancer</subject><subject>Cell Survival - drug effects</subject><subject>Colon</subject><subject>Colorectal cancer</subject><subject>Cytotoxicity</subject><subject>Histology</subject><subject>HT29 Cells - drug effects</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>Lung cancer</subject><subject>Metal Nanoparticles - adverse effects</subject><subject>Morphology</subject><subject>Nanoparticles</subject><subject>Original Article</subject><subject>Plant Extracts - pharmacology</subject><subject>Prosopis - adverse effects</subject><subject>Prosopis farcta</subject><subject>Silver</subject><subject>Silver - adverse effects</subject><subject>Silver - metabolism</subject><subject>Spectrophotometry, Ultraviolet</subject><subject>X-Ray Diffraction</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kE1P3DAQhi1UBNttf0AvKFIvXFJm_JHEx3YFBWklOMDZcmJna5SNt54ECX49Xi20UqWe5jDPvDPzMPYF4RsC1BeECEKUgE0pudYlHLEFqlqUUtfNB7YAAVjKRuEp-0j0CAASa3XCTgVwLVDzBVv_CHGIm9DZoaBpdsFTEfuCnsfpl6fw4l1BYXjyqRjtGHc2TaEbMjNTGDfFXYoUd4GK3qZusp_YcW8H8p_f6pI9XF3er67L9e3Pm9X3ddmJmk-lQsc7aGsEpwR3TaNaK4XklYPe8b5SstK5LZ1ubI-yxdZ1WnMnQaGVloslOz_k7lL8PXuazDZQ54fBjj7OZDhijarhfI9-_Qd9jHMa83V7qqqUANlkCg9Ulx-i5HuzS2Fr07NBMHvV5qDaZNVmr9pAnjl7S57brXd_Jt7dZoAfAMqtcePT39X_T30F33WIfg</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Miri, Abdolhossien</creator><creator>Sarani, Mina</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20181201</creationdate><title>Biological studies of synthesized silver nanoparticles using Prosopis farcta</title><author>Miri, Abdolhossien ; 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Prosopis farcta
was investigated against lung (A549) and colon (HT-29) cell lines. The cytotoxic activity of nanoparticles was performed using MTT assay, while their apoptotic activity was tested using TUNEL method. The obtained results of MTT showed that the cell viability of A549 was dependent on the nanoparticles concentration and incubation time. Therefore, although the cytotoxic effect increased as the Ag-NPs concentration and incubation time heightened, yet the viability of HT-29 cells seems to be dependent only on the incubation time. The apoptotic results of the nanoparticles showed more than 50% of apoptosis on A549 and HT-29 cell lines, which in this case, HT-29 demonstrated 100% apoptosis at concentrations of more than 400 µg/ml. It seems that Ag-NPs synthesized using
P. farcta
extract can serve as anti-cancer agent in the treatment many cancers through creating or discovering new drug forms.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30293192</pmid><doi>10.1007/s11033-018-4299-0</doi><tpages>6</tpages></addata></record> |
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| subjects | A549 Cells - drug effects Animal Anatomy Animal Biochemistry Apoptosis Apoptosis - drug effects Biomedical and Life Sciences Cancer Cell Survival - drug effects Colon Colorectal cancer Cytotoxicity Histology HT29 Cells - drug effects Humans Life Sciences Lung cancer Metal Nanoparticles - adverse effects Morphology Nanoparticles Original Article Plant Extracts - pharmacology Prosopis - adverse effects Prosopis farcta Silver Silver - adverse effects Silver - metabolism Spectrophotometry, Ultraviolet X-Ray Diffraction |
| title | Biological studies of synthesized silver nanoparticles using Prosopis farcta |
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