Antibiotic That Inhibits the ATPase Activity of an ATP-Binding Cassette Transporter by Binding to a Remote Extracellular Site
Antibiotic-resistant strains of Staphylococcus aureus pose a major threat to human health and there is an ongoing need for new antibiotics to treat resistant infections. In a high throughput screen (HTS) of 230 000 small molecules designed to identify bioactive wall teichoic acid (WTA) inhibitors, w...
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| Veröffentlicht in: | Journal of the American Chemical Society 2017-08, Vol.139 (31), p.10597-10600 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 139 |
| creator | Matano, Leigh M Morris, Heidi G Hesser, Anthony R Martin, Sara E. S Lee, Wonsik Owens, Tristan W Laney, Emaline Nakaminami, Hidemasa Hooper, David Meredith, Timothy C Walker, Suzanne |
| description | Antibiotic-resistant strains of Staphylococcus aureus pose a major threat to human health and there is an ongoing need for new antibiotics to treat resistant infections. In a high throughput screen (HTS) of 230 000 small molecules designed to identify bioactive wall teichoic acid (WTA) inhibitors, we identified one hit, which was expanded through chemical synthesis into a small panel of potent compounds. We showed that these compounds target TarG, the transmembrane component of the two-component ATP-binding cassette (ABC) transporter TarGH, which exports WTA precursors to the cell surface for attachment to peptidoglycan. We purified, for the first time, a WTA transporter and have reconstituted ATPase activity in proteoliposomes. We showed that this new compound series inhibits TarH-catalyzed ATP hydrolysis even though the binding site maps to TarG near the opposite side of the membrane. These are the first ABC transporter inhibitors shown to block ATPase activity by binding to the transmembrane domain. The compounds have potential as therapeutic agents to treat S. aureus infections, and purification of the transmembrane transporter will enable further development. |
| doi_str_mv | 10.1021/jacs.7b04726 |
| format | Article |
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We showed that these compounds target TarG, the transmembrane component of the two-component ATP-binding cassette (ABC) transporter TarGH, which exports WTA precursors to the cell surface for attachment to peptidoglycan. We purified, for the first time, a WTA transporter and have reconstituted ATPase activity in proteoliposomes. We showed that this new compound series inhibits TarH-catalyzed ATP hydrolysis even though the binding site maps to TarG near the opposite side of the membrane. These are the first ABC transporter inhibitors shown to block ATPase activity by binding to the transmembrane domain. 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In a high throughput screen (HTS) of 230 000 small molecules designed to identify bioactive wall teichoic acid (WTA) inhibitors, we identified one hit, which was expanded through chemical synthesis into a small panel of potent compounds. We showed that these compounds target TarG, the transmembrane component of the two-component ATP-binding cassette (ABC) transporter TarGH, which exports WTA precursors to the cell surface for attachment to peptidoglycan. We purified, for the first time, a WTA transporter and have reconstituted ATPase activity in proteoliposomes. We showed that this new compound series inhibits TarH-catalyzed ATP hydrolysis even though the binding site maps to TarG near the opposite side of the membrane. These are the first ABC transporter inhibitors shown to block ATPase activity by binding to the transmembrane domain. 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S</au><au>Lee, Wonsik</au><au>Owens, Tristan W</au><au>Laney, Emaline</au><au>Nakaminami, Hidemasa</au><au>Hooper, David</au><au>Meredith, Timothy C</au><au>Walker, Suzanne</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibiotic That Inhibits the ATPase Activity of an ATP-Binding Cassette Transporter by Binding to a Remote Extracellular Site</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2017-08-09</date><risdate>2017</risdate><volume>139</volume><issue>31</issue><spage>10597</spage><epage>10600</epage><pages>10597-10600</pages><issn>0002-7863</issn><issn>1520-5126</issn><eissn>1520-5126</eissn><abstract>Antibiotic-resistant strains of Staphylococcus aureus pose a major threat to human health and there is an ongoing need for new antibiotics to treat resistant infections. 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| ispartof | Journal of the American Chemical Society, 2017-08, Vol.139 (31), p.10597-10600 |
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| subjects | ABC transporters Adenosine Triphosphatases - antagonists & inhibitors adenosine triphosphate adenosinetriphosphatase antibiotic resistance antibiotics ATP-Binding Cassette Transporters - metabolism Binding Sites Cell Wall - chemistry Cell Wall - drug effects Cell Wall - metabolism Drug Delivery Systems Drug Evaluation, Preclinical Enzyme Activation - drug effects enzyme activity enzyme inhibition hydrolysis Methicillin-Resistant Staphylococcus aureus - drug effects Models, Biological Molecular Structure Peptidoglycan - chemistry Peptidoglycan - metabolism peptidoglycans Protein Binding - drug effects Staphylococcus aureus Staphylococcus aureus - drug effects synthesis teichoic acids Teichoic Acids - pharmacology therapeutics |
| title | Antibiotic That Inhibits the ATPase Activity of an ATP-Binding Cassette Transporter by Binding to a Remote Extracellular Site |
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