Quantification of Toxoplasma gondii in the tissues of BALB/c mice after immunization with nanoliposomal excretory-secretory antigens using Real-Time PCR
[Display omitted] •A nanoliposomal structure containing Toxoplasma gondii ESA was constructed.•We monitored the Toxoplasma gondii parasite load using QPCR in blood, spleen, and brain tissues.•NLESAs showed decreased parasite load in tissues of immunized mice compared to control mice. Toxoplasmosis i...
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| Veröffentlicht in: | Comparative immunology, microbiology and infectious diseases microbiology and infectious diseases, 2018-08, Vol.59, p.52-56 |
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| creator | Azadi, Yaghob Ahmadpour, Ehsan Hamishehkar, Hamed Daryani, Ahmad Spotin, Adel Mahami-Oskouei, Mahmoud Barac, Aleksandra Rajabi, Saba Alizadeh, Paria Montazeri, Mahbobeh |
| description | [Display omitted]
•A nanoliposomal structure containing Toxoplasma gondii ESA was constructed.•We monitored the Toxoplasma gondii parasite load using QPCR in blood, spleen, and brain tissues.•NLESAs showed decreased parasite load in tissues of immunized mice compared to control mice.
Toxoplasmosis is an infectious disease caused by the intracellular parasite Toxoplasma gondii. Although almost 1/3 of the world’s population are seropositive, there is no effective vaccine against toxoplasmosis. Therefore, the development of an effective vaccine for control of toxoplasmosis is one of major concerns in parasitology. The aim of this study was to evaluate the efficacy of nano-liposomal excretory-secretory antigens (NLESA) in BALB/c mice.
Excretory-secretory antigens (ESA) was obtained from tachyzoites, encapsulated in the liposome and studied by scanning electron microscope. BALB/c mice were immunized with NLESA and ESA, sterile phosphate-buffered saline (PBS). Immunization was performed three times at 14-day intervals and challenged with 1 × 104 tachyzoites of T. gondii RH strain four weeks later. The parasite load of mice blood, brain and spleen tissues were determined using quantitative PCR targeted at the repeated element (RE) gene.
The immunization with NLESA and ESA induced a significant increase of anti-Toxoplasma IgG antibody compared with PBS group (P |
| doi_str_mv | 10.1016/j.cimid.2018.09.012 |
| format | Article |
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•A nanoliposomal structure containing Toxoplasma gondii ESA was constructed.•We monitored the Toxoplasma gondii parasite load using QPCR in blood, spleen, and brain tissues.•NLESAs showed decreased parasite load in tissues of immunized mice compared to control mice.
Toxoplasmosis is an infectious disease caused by the intracellular parasite Toxoplasma gondii. Although almost 1/3 of the world’s population are seropositive, there is no effective vaccine against toxoplasmosis. Therefore, the development of an effective vaccine for control of toxoplasmosis is one of major concerns in parasitology. The aim of this study was to evaluate the efficacy of nano-liposomal excretory-secretory antigens (NLESA) in BALB/c mice.
Excretory-secretory antigens (ESA) was obtained from tachyzoites, encapsulated in the liposome and studied by scanning electron microscope. BALB/c mice were immunized with NLESA and ESA, sterile phosphate-buffered saline (PBS). Immunization was performed three times at 14-day intervals and challenged with 1 × 104 tachyzoites of T. gondii RH strain four weeks later. The parasite load of mice blood, brain and spleen tissues were determined using quantitative PCR targeted at the repeated element (RE) gene.
The immunization with NLESA and ESA induced a significant increase of anti-Toxoplasma IgG antibody compared with PBS group (P < 0.05). After challenge with tachyzoites, qPCR analyses showed significant reduction of parasite load in NLESA and ESA immunized mice compared with control group (P < 0.05). Also, NLESAs were more effective than ESAs and showed significantly reduced parasite load in blood (P = 0.001) and brain tissue (P = 0.01).
The vaccination with NLESA showed more promising results comparing to ESA. Further studies are recommended in order to achieve effectiveness of the vaccine against T. gondii.</description><identifier>ISSN: 0147-9571</identifier><identifier>EISSN: 1878-1667</identifier><identifier>DOI: 10.1016/j.cimid.2018.09.012</identifier><identifier>PMID: 30290888</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Antibodies, Protozoan - immunology ; Antigens, Protozoan - immunology ; Excretory-secretory antigens ; Female ; Immunization - methods ; Liposome ; Liposomes - immunology ; Mice ; Mice, Inbred BALB C ; Nanoparticles - administration & dosage ; Parasite load ; Parasite Load - methods ; Protozoan Vaccines - immunology ; Real-time PCR ; Real-Time Polymerase Chain Reaction - methods ; Toxoplasma - genetics ; Toxoplasma - immunology ; Toxoplasma gondii ; Toxoplasmosis, Animal - immunology ; Toxoplasmosis, Animal - parasitology ; Vaccination - methods ; Vaccine</subject><ispartof>Comparative immunology, microbiology and infectious diseases, 2018-08, Vol.59, p.52-56</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-1e78456a80d31739891acac3614285ab83fedf751d0b99fca6af5696ebd5be5e3</citedby><cites>FETCH-LOGICAL-c359t-1e78456a80d31739891acac3614285ab83fedf751d0b99fca6af5696ebd5be5e3</cites><orcidid>0000-0001-8571-5803 ; 0000-0002-0132-2277 ; 0000-0003-1202-6147</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cimid.2018.09.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3552,27931,27932,46002</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30290888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Azadi, Yaghob</creatorcontrib><creatorcontrib>Ahmadpour, Ehsan</creatorcontrib><creatorcontrib>Hamishehkar, Hamed</creatorcontrib><creatorcontrib>Daryani, Ahmad</creatorcontrib><creatorcontrib>Spotin, Adel</creatorcontrib><creatorcontrib>Mahami-Oskouei, Mahmoud</creatorcontrib><creatorcontrib>Barac, Aleksandra</creatorcontrib><creatorcontrib>Rajabi, Saba</creatorcontrib><creatorcontrib>Alizadeh, Paria</creatorcontrib><creatorcontrib>Montazeri, Mahbobeh</creatorcontrib><title>Quantification of Toxoplasma gondii in the tissues of BALB/c mice after immunization with nanoliposomal excretory-secretory antigens using Real-Time PCR</title><title>Comparative immunology, microbiology and infectious diseases</title><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><description>[Display omitted]
•A nanoliposomal structure containing Toxoplasma gondii ESA was constructed.•We monitored the Toxoplasma gondii parasite load using QPCR in blood, spleen, and brain tissues.•NLESAs showed decreased parasite load in tissues of immunized mice compared to control mice.
Toxoplasmosis is an infectious disease caused by the intracellular parasite Toxoplasma gondii. Although almost 1/3 of the world’s population are seropositive, there is no effective vaccine against toxoplasmosis. Therefore, the development of an effective vaccine for control of toxoplasmosis is one of major concerns in parasitology. The aim of this study was to evaluate the efficacy of nano-liposomal excretory-secretory antigens (NLESA) in BALB/c mice.
Excretory-secretory antigens (ESA) was obtained from tachyzoites, encapsulated in the liposome and studied by scanning electron microscope. BALB/c mice were immunized with NLESA and ESA, sterile phosphate-buffered saline (PBS). Immunization was performed three times at 14-day intervals and challenged with 1 × 104 tachyzoites of T. gondii RH strain four weeks later. The parasite load of mice blood, brain and spleen tissues were determined using quantitative PCR targeted at the repeated element (RE) gene.
The immunization with NLESA and ESA induced a significant increase of anti-Toxoplasma IgG antibody compared with PBS group (P < 0.05). After challenge with tachyzoites, qPCR analyses showed significant reduction of parasite load in NLESA and ESA immunized mice compared with control group (P < 0.05). Also, NLESAs were more effective than ESAs and showed significantly reduced parasite load in blood (P = 0.001) and brain tissue (P = 0.01).
The vaccination with NLESA showed more promising results comparing to ESA. Further studies are recommended in order to achieve effectiveness of the vaccine against T. gondii.</description><subject>Animals</subject><subject>Antibodies, Protozoan - immunology</subject><subject>Antigens, Protozoan - immunology</subject><subject>Excretory-secretory antigens</subject><subject>Female</subject><subject>Immunization - methods</subject><subject>Liposome</subject><subject>Liposomes - immunology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Nanoparticles - administration & dosage</subject><subject>Parasite load</subject><subject>Parasite Load - methods</subject><subject>Protozoan Vaccines - immunology</subject><subject>Real-time PCR</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Toxoplasma - genetics</subject><subject>Toxoplasma - immunology</subject><subject>Toxoplasma gondii</subject><subject>Toxoplasmosis, Animal - immunology</subject><subject>Toxoplasmosis, Animal - parasitology</subject><subject>Vaccination - methods</subject><subject>Vaccine</subject><issn>0147-9571</issn><issn>1878-1667</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU2P0zAQhiMEYsvCL0BCPnJJ1pNP-8Bht-JLqgSsytlynEl3qtgutgO7_BJ-LiktHDnNHJ53Xo2eLHsJvAAO7dW-MGRpKEoOouCy4FA-ylYgOpFD23aPsxWHustl08FF9izGPedcQg1Ps4uKl5ILIVbZry-zdolGMjqRd8yPbOvv_WHS0Wq2824gYuRYukOWKMYZ45G5ud7cXBlmySDTY8LAyNrZ0c_TlR-U7pjTzk908NFbPTG8NwGTDw95xPPGjs07dJHNkdyO3aKe8i1ZZJ_Xt8-zJ6OeIr44z8vs67u32_WHfPPp_cf19SY3VSNTDtiJumm14EMFXSWFBG20qVqoS9HoXlQjDmPXwMB7KUejWz02rWyxH5oeG6wus9enu4fgvy3fJWUpGpwm7dDPUZUArag7aMoFrU6oCT7GgKM6BLI6PCjg6qhE7dUfJeqoRHGpFiVL6tW5YO4tDv8yfx0swJsTgMub3wmDiobQGRwooElq8PTfgt_MuqFc</recordid><startdate>201808</startdate><enddate>201808</enddate><creator>Azadi, Yaghob</creator><creator>Ahmadpour, Ehsan</creator><creator>Hamishehkar, Hamed</creator><creator>Daryani, Ahmad</creator><creator>Spotin, Adel</creator><creator>Mahami-Oskouei, Mahmoud</creator><creator>Barac, Aleksandra</creator><creator>Rajabi, Saba</creator><creator>Alizadeh, Paria</creator><creator>Montazeri, Mahbobeh</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-8571-5803</orcidid><orcidid>https://orcid.org/0000-0002-0132-2277</orcidid><orcidid>https://orcid.org/0000-0003-1202-6147</orcidid></search><sort><creationdate>201808</creationdate><title>Quantification of Toxoplasma gondii in the tissues of BALB/c mice after immunization with nanoliposomal excretory-secretory antigens using Real-Time PCR</title><author>Azadi, Yaghob ; Ahmadpour, Ehsan ; Hamishehkar, Hamed ; Daryani, Ahmad ; Spotin, Adel ; Mahami-Oskouei, Mahmoud ; Barac, Aleksandra ; Rajabi, Saba ; Alizadeh, Paria ; Montazeri, Mahbobeh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-1e78456a80d31739891acac3614285ab83fedf751d0b99fca6af5696ebd5be5e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Antibodies, Protozoan - immunology</topic><topic>Antigens, Protozoan - immunology</topic><topic>Excretory-secretory antigens</topic><topic>Female</topic><topic>Immunization - methods</topic><topic>Liposome</topic><topic>Liposomes - immunology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Nanoparticles - administration & dosage</topic><topic>Parasite load</topic><topic>Parasite Load - methods</topic><topic>Protozoan Vaccines - immunology</topic><topic>Real-time PCR</topic><topic>Real-Time Polymerase Chain Reaction - methods</topic><topic>Toxoplasma - genetics</topic><topic>Toxoplasma - immunology</topic><topic>Toxoplasma gondii</topic><topic>Toxoplasmosis, Animal - immunology</topic><topic>Toxoplasmosis, Animal - parasitology</topic><topic>Vaccination - methods</topic><topic>Vaccine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Azadi, Yaghob</creatorcontrib><creatorcontrib>Ahmadpour, Ehsan</creatorcontrib><creatorcontrib>Hamishehkar, Hamed</creatorcontrib><creatorcontrib>Daryani, Ahmad</creatorcontrib><creatorcontrib>Spotin, Adel</creatorcontrib><creatorcontrib>Mahami-Oskouei, Mahmoud</creatorcontrib><creatorcontrib>Barac, Aleksandra</creatorcontrib><creatorcontrib>Rajabi, Saba</creatorcontrib><creatorcontrib>Alizadeh, Paria</creatorcontrib><creatorcontrib>Montazeri, Mahbobeh</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Azadi, Yaghob</au><au>Ahmadpour, Ehsan</au><au>Hamishehkar, Hamed</au><au>Daryani, Ahmad</au><au>Spotin, Adel</au><au>Mahami-Oskouei, Mahmoud</au><au>Barac, Aleksandra</au><au>Rajabi, Saba</au><au>Alizadeh, Paria</au><au>Montazeri, Mahbobeh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantification of Toxoplasma gondii in the tissues of BALB/c mice after immunization with nanoliposomal excretory-secretory antigens using Real-Time PCR</atitle><jtitle>Comparative immunology, microbiology and infectious diseases</jtitle><addtitle>Comp Immunol Microbiol Infect Dis</addtitle><date>2018-08</date><risdate>2018</risdate><volume>59</volume><spage>52</spage><epage>56</epage><pages>52-56</pages><issn>0147-9571</issn><eissn>1878-1667</eissn><abstract>[Display omitted]
•A nanoliposomal structure containing Toxoplasma gondii ESA was constructed.•We monitored the Toxoplasma gondii parasite load using QPCR in blood, spleen, and brain tissues.•NLESAs showed decreased parasite load in tissues of immunized mice compared to control mice.
Toxoplasmosis is an infectious disease caused by the intracellular parasite Toxoplasma gondii. Although almost 1/3 of the world’s population are seropositive, there is no effective vaccine against toxoplasmosis. Therefore, the development of an effective vaccine for control of toxoplasmosis is one of major concerns in parasitology. The aim of this study was to evaluate the efficacy of nano-liposomal excretory-secretory antigens (NLESA) in BALB/c mice.
Excretory-secretory antigens (ESA) was obtained from tachyzoites, encapsulated in the liposome and studied by scanning electron microscope. BALB/c mice were immunized with NLESA and ESA, sterile phosphate-buffered saline (PBS). Immunization was performed three times at 14-day intervals and challenged with 1 × 104 tachyzoites of T. gondii RH strain four weeks later. The parasite load of mice blood, brain and spleen tissues were determined using quantitative PCR targeted at the repeated element (RE) gene.
The immunization with NLESA and ESA induced a significant increase of anti-Toxoplasma IgG antibody compared with PBS group (P < 0.05). After challenge with tachyzoites, qPCR analyses showed significant reduction of parasite load in NLESA and ESA immunized mice compared with control group (P < 0.05). Also, NLESAs were more effective than ESAs and showed significantly reduced parasite load in blood (P = 0.001) and brain tissue (P = 0.01).
The vaccination with NLESA showed more promising results comparing to ESA. Further studies are recommended in order to achieve effectiveness of the vaccine against T. gondii.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>30290888</pmid><doi>10.1016/j.cimid.2018.09.012</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0001-8571-5803</orcidid><orcidid>https://orcid.org/0000-0002-0132-2277</orcidid><orcidid>https://orcid.org/0000-0003-1202-6147</orcidid></addata></record> |
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| subjects | Animals Antibodies, Protozoan - immunology Antigens, Protozoan - immunology Excretory-secretory antigens Female Immunization - methods Liposome Liposomes - immunology Mice Mice, Inbred BALB C Nanoparticles - administration & dosage Parasite load Parasite Load - methods Protozoan Vaccines - immunology Real-time PCR Real-Time Polymerase Chain Reaction - methods Toxoplasma - genetics Toxoplasma - immunology Toxoplasma gondii Toxoplasmosis, Animal - immunology Toxoplasmosis, Animal - parasitology Vaccination - methods Vaccine |
| title | Quantification of Toxoplasma gondii in the tissues of BALB/c mice after immunization with nanoliposomal excretory-secretory antigens using Real-Time PCR |
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