Focus on recently developed assays for detection of resistance/sensitivity to reverse transcriptase inhibitors
The biology of HIV is rather complex due to high rate of replication, frequent recombination, and introduction of mutations. This gives rise to a number of distinct variants referred as quasispecies. In addition, the latency within reservoir allows the periodic reactivation of virus replication. The...
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| Veröffentlicht in: | Applied microbiology and biotechnology 2018-12, Vol.102 (23), p.9925-9936 |
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| creator | Marino-Merlo, Francesca Macchi, Beatrice Armenia, Daniele Bellocchi, Maria Concetta Ceccherini-Silberstein, Francesca Mastino, Antonio Grelli, Sandro |
| description | The biology of HIV is rather complex due to high rate of replication, frequent recombination, and introduction of mutations. This gives rise to a number of distinct variants referred as quasispecies. In addition, the latency within reservoir allows the periodic reactivation of virus replication. The rapid replication of HIV allows immune response escape and establishment of resistance to therapy that can be acquired through drug selection and/or transmitted among individuals. This prompted, over the years, the development of a range of assays aimed to determine drug resistance and sensitivity, to be used both in clinical practice and in antiviral research. Reverse transcriptase (RT) inhibitors have an eminent place among the anti-HIV drugs, being constantly present from the beginning until today in the most commonly used antiviral regimens. This mini-review seeks to provide an up-to-date overview of recent efforts in developing even more reliable and simple methods, of both genotypic and phenotypic types, for specifically detecting drug resistance and sensitivity to RT inhibitors. |
| doi_str_mv | 10.1007/s00253-018-9390-x |
| format | Article |
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All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-76b8bc3031659eb52194f51bf53b31074de1c6b0b9d0620dec6d83198185cce93</citedby><cites>FETCH-LOGICAL-c372t-76b8bc3031659eb52194f51bf53b31074de1c6b0b9d0620dec6d83198185cce93</cites><orcidid>0000-0002-0338-6450</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00253-018-9390-x$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00253-018-9390-x$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30269214$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Marino-Merlo, Francesca</creatorcontrib><creatorcontrib>Macchi, Beatrice</creatorcontrib><creatorcontrib>Armenia, Daniele</creatorcontrib><creatorcontrib>Bellocchi, Maria Concetta</creatorcontrib><creatorcontrib>Ceccherini-Silberstein, Francesca</creatorcontrib><creatorcontrib>Mastino, Antonio</creatorcontrib><creatorcontrib>Grelli, Sandro</creatorcontrib><title>Focus on recently developed assays for detection of resistance/sensitivity to reverse transcriptase inhibitors</title><title>Applied microbiology and biotechnology</title><addtitle>Appl Microbiol Biotechnol</addtitle><addtitle>Appl Microbiol Biotechnol</addtitle><description>The biology of HIV is rather complex due to high rate of replication, frequent recombination, and introduction of mutations. 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| subjects | Activation Antiviral agents Biomedical and Life Sciences Biotechnology Diagnostic Tests, Routine Drug resistance Drug Resistance, Multiple, Viral Genotyping Techniques HIV HIV Infections - drug therapy HIV-1 - drug effects HIV-1 - physiology Human immunodeficiency virus Humans Immune response Immune system Immunosuppressive agents Inhibitors Latency Life Sciences Microbial Genetics and Genomics Microbiology Mini-Review Mutation Recombination Replication Reverse Transcriptase Inhibitors - pharmacology Reviews RNA-directed DNA polymerase Sensitivity Virus Replication Viruses |
| title | Focus on recently developed assays for detection of resistance/sensitivity to reverse transcriptase inhibitors |
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