Comparative Analysis of Genetic and Chemical Differences between Four Berberis Herbs Based on Molecular Phylogenetic and HPLC Methods
In traditional Tibetan medicinal system, Berberis herbs mainly originate from the dried barks of Berberis kansuensis, Berberis dictyophylla, Berberis diaphana, and Berberis vernae. In this study, molecular phylogenetic method based on four markers (i.e., rbcL, internal transcribed spacer (ITS), ITS2...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2018/12/01, Vol.41(12), pp.1870-1873 |
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| creator | Feng, Tu Du, Huan Chen, Hanting Xiao, Qunying He, Yang Fan, Gang |
| description | In traditional Tibetan medicinal system, Berberis herbs mainly originate from the dried barks of Berberis kansuensis, Berberis dictyophylla, Berberis diaphana, and Berberis vernae. In this study, molecular phylogenetic method based on four markers (i.e., rbcL, internal transcribed spacer (ITS), ITS2, and psbA-trnH) and HPLC chemical analysis were used to evaluate the chemical and genetic differences between the four Berberis species. The results showed that the discriminatory power of ITS, ITS2 and psbA-trnH was low, but the rbcL marker was highly effective and reliable for the species differentiation. The four Berberis species can be successfully classified based on phylogenetic analysis of the rbcL sequences. Moreover, the results of chemical analysis showed that four main alkaloids (i.e., berberine, palmatine, magnoflorine, and jatrorrhizine) cannot be used as chemical markers for discrimination of the four Berberis species. These findings provide valuable information for distinguishing the four Berberis Tibetan herbs. |
| doi_str_mv | 10.1248/bpb.b18-00327 |
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In this study, molecular phylogenetic method based on four markers (i.e., rbcL, internal transcribed spacer (ITS), ITS2, and psbA-trnH) and HPLC chemical analysis were used to evaluate the chemical and genetic differences between the four Berberis species. The results showed that the discriminatory power of ITS, ITS2 and psbA-trnH was low, but the rbcL marker was highly effective and reliable for the species differentiation. The four Berberis species can be successfully classified based on phylogenetic analysis of the rbcL sequences. Moreover, the results of chemical analysis showed that four main alkaloids (i.e., berberine, palmatine, magnoflorine, and jatrorrhizine) cannot be used as chemical markers for discrimination of the four Berberis species. These findings provide valuable information for distinguishing the four Berberis Tibetan herbs.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.b18-00327</identifier><identifier>PMID: 30259882</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>alkaloid ; Alkaloids ; Alkaloids - analysis ; Alkaloids - genetics ; Analytical chemistry ; Berberine ; Berberis ; Berberis - chemistry ; Berberis - genetics ; Berberis herb ; Chemical analysis ; Chromatography, High Pressure Liquid - methods ; Genetic analysis ; Herbal medicine ; Herbs ; High-performance liquid chromatography ; interspecies variation ; Liquid chromatography ; molecular phylogenetic method ; Phylogenetics ; Phylogeny ; Plant Preparations - chemistry ; Plants, Medicinal - chemistry ; Plants, Medicinal - genetics ; Spacer ; Species ; species differentiation</subject><ispartof>Biological and Pharmaceutical Bulletin, 2018/12/01, Vol.41(12), pp.1870-1873</ispartof><rights>2018 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c703t-fbbea5e3cd24720def7f53023cc63f274d45b1916b3a7759ab7cb9a54ed83a783</citedby><cites>FETCH-LOGICAL-c703t-fbbea5e3cd24720def7f53023cc63f274d45b1916b3a7759ab7cb9a54ed83a783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30259882$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feng, Tu</creatorcontrib><creatorcontrib>Du, Huan</creatorcontrib><creatorcontrib>Chen, Hanting</creatorcontrib><creatorcontrib>Xiao, Qunying</creatorcontrib><creatorcontrib>He, Yang</creatorcontrib><creatorcontrib>Fan, Gang</creatorcontrib><creatorcontrib>Collaborative Innovation Center of Wetland Eco-engineering of Guizhou Province</creatorcontrib><creatorcontrib>aSchool of Ecological Engineering</creatorcontrib><creatorcontrib>bSchool of Ethnic Medicine</creatorcontrib><creatorcontrib>Key Laboratory of Biological Resources and Ecological Remediation of Guizhou Province</creatorcontrib><creatorcontrib>Chengdu University of Traditional Chinese Medicine</creatorcontrib><creatorcontrib>Guizhou University of Engineering Science</creatorcontrib><creatorcontrib>cSchool of Medical Technology</creatorcontrib><title>Comparative Analysis of Genetic and Chemical Differences between Four Berberis Herbs Based on Molecular Phylogenetic and HPLC Methods</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>In traditional Tibetan medicinal system, Berberis herbs mainly originate from the dried barks of Berberis kansuensis, Berberis dictyophylla, Berberis diaphana, and Berberis vernae. 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These findings provide valuable information for distinguishing the four Berberis Tibetan herbs.</description><subject>alkaloid</subject><subject>Alkaloids</subject><subject>Alkaloids - analysis</subject><subject>Alkaloids - genetics</subject><subject>Analytical chemistry</subject><subject>Berberine</subject><subject>Berberis</subject><subject>Berberis - chemistry</subject><subject>Berberis - genetics</subject><subject>Berberis herb</subject><subject>Chemical analysis</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Genetic analysis</subject><subject>Herbal medicine</subject><subject>Herbs</subject><subject>High-performance liquid chromatography</subject><subject>interspecies variation</subject><subject>Liquid chromatography</subject><subject>molecular phylogenetic method</subject><subject>Phylogenetics</subject><subject>Phylogeny</subject><subject>Plant Preparations - chemistry</subject><subject>Plants, Medicinal - chemistry</subject><subject>Plants, Medicinal - genetics</subject><subject>Spacer</subject><subject>Species</subject><subject>species differentiation</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkU2P0zAQhiMEYsvCkSuyxIVLFn_EtXPczbItUlfsAc6W7Uy2qRy72AmoP4D_jdMuBSHZY8vz-PV43qJ4S_AVoZX8aPbmyhBZYsyoeFYsCKtEySnhz4sFrnNiSbi8KF6ltMMYC0zZy-KCYcprKemi-NWEYa-jHvsfgK69dofUJxQ6tAIPY2-R9i1qtjD0Vjt023cdRPAWEjIw_gTw6C5MEd1ANBDzzXXeJHSjE7QoeHQfHNjJ6YgetgcXHv8RXT9sGnQP4za06XXxotMuwZun9bL4dvfpa7MuN19Wn5vrTWkFZmPZGQOaA7MtrQTFLXSi4_kvzNol66io2oobUpOlYVoIXmsjrKk1r6CV-USyy-LDSXcfw_cJ0qiGPllwTnsIU1KUEEaXFFOS0ff_obv80dygTNEqD55npsoTZWNIKUKn9rEfdDwogtXsj8r-qOyPOvqT-XdPqpMZoD3TfwzJwOoE5Ozc8uBd7-Hv2zYJ0wcXFMVH0YoQqjCpFZECz4ExIiWuZ6Xbk9IujfoRzk_pmB1wcCysIrnIOZ4rPKftVkcFnv0GThq6Yw</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Feng, Tu</creator><creator>Du, Huan</creator><creator>Chen, Hanting</creator><creator>Xiao, Qunying</creator><creator>He, Yang</creator><creator>Fan, Gang</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20181201</creationdate><title>Comparative Analysis of Genetic and Chemical Differences between Four Berberis Herbs Based on Molecular Phylogenetic and HPLC Methods</title><author>Feng, Tu ; Du, Huan ; Chen, Hanting ; Xiao, Qunying ; He, Yang ; Fan, Gang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c703t-fbbea5e3cd24720def7f53023cc63f274d45b1916b3a7759ab7cb9a54ed83a783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>alkaloid</topic><topic>Alkaloids</topic><topic>Alkaloids - analysis</topic><topic>Alkaloids - genetics</topic><topic>Analytical chemistry</topic><topic>Berberine</topic><topic>Berberis</topic><topic>Berberis - chemistry</topic><topic>Berberis - genetics</topic><topic>Berberis herb</topic><topic>Chemical analysis</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Genetic analysis</topic><topic>Herbal medicine</topic><topic>Herbs</topic><topic>High-performance liquid chromatography</topic><topic>interspecies variation</topic><topic>Liquid chromatography</topic><topic>molecular phylogenetic method</topic><topic>Phylogenetics</topic><topic>Phylogeny</topic><topic>Plant Preparations - chemistry</topic><topic>Plants, Medicinal - chemistry</topic><topic>Plants, Medicinal - genetics</topic><topic>Spacer</topic><topic>Species</topic><topic>species differentiation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Feng, Tu</creatorcontrib><creatorcontrib>Du, Huan</creatorcontrib><creatorcontrib>Chen, Hanting</creatorcontrib><creatorcontrib>Xiao, Qunying</creatorcontrib><creatorcontrib>He, Yang</creatorcontrib><creatorcontrib>Fan, Gang</creatorcontrib><creatorcontrib>Collaborative Innovation Center of Wetland Eco-engineering of Guizhou Province</creatorcontrib><creatorcontrib>aSchool of Ecological Engineering</creatorcontrib><creatorcontrib>bSchool of Ethnic Medicine</creatorcontrib><creatorcontrib>Key Laboratory of Biological Resources and Ecological Remediation of Guizhou Province</creatorcontrib><creatorcontrib>Chengdu University of Traditional Chinese Medicine</creatorcontrib><creatorcontrib>Guizhou University of Engineering Science</creatorcontrib><creatorcontrib>cSchool of Medical Technology</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Feng, Tu</au><au>Du, Huan</au><au>Chen, Hanting</au><au>Xiao, Qunying</au><au>He, Yang</au><au>Fan, Gang</au><aucorp>Collaborative Innovation Center of Wetland Eco-engineering of Guizhou Province</aucorp><aucorp>aSchool of Ecological Engineering</aucorp><aucorp>bSchool of Ethnic Medicine</aucorp><aucorp>Key Laboratory of Biological Resources and Ecological Remediation of Guizhou Province</aucorp><aucorp>Chengdu University of Traditional Chinese Medicine</aucorp><aucorp>Guizhou University of Engineering Science</aucorp><aucorp>cSchool of Medical Technology</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative Analysis of Genetic and Chemical Differences between Four Berberis Herbs Based on Molecular Phylogenetic and HPLC Methods</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2018-12-01</date><risdate>2018</risdate><volume>41</volume><issue>12</issue><spage>1870</spage><epage>1873</epage><pages>1870-1873</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>In traditional Tibetan medicinal system, Berberis herbs mainly originate from the dried barks of Berberis kansuensis, Berberis dictyophylla, Berberis diaphana, and Berberis vernae. In this study, molecular phylogenetic method based on four markers (i.e., rbcL, internal transcribed spacer (ITS), ITS2, and psbA-trnH) and HPLC chemical analysis were used to evaluate the chemical and genetic differences between the four Berberis species. The results showed that the discriminatory power of ITS, ITS2 and psbA-trnH was low, but the rbcL marker was highly effective and reliable for the species differentiation. The four Berberis species can be successfully classified based on phylogenetic analysis of the rbcL sequences. Moreover, the results of chemical analysis showed that four main alkaloids (i.e., berberine, palmatine, magnoflorine, and jatrorrhizine) cannot be used as chemical markers for discrimination of the four Berberis species. These findings provide valuable information for distinguishing the four Berberis Tibetan herbs.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>30259882</pmid><doi>10.1248/bpb.b18-00327</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | alkaloid Alkaloids Alkaloids - analysis Alkaloids - genetics Analytical chemistry Berberine Berberis Berberis - chemistry Berberis - genetics Berberis herb Chemical analysis Chromatography, High Pressure Liquid - methods Genetic analysis Herbal medicine Herbs High-performance liquid chromatography interspecies variation Liquid chromatography molecular phylogenetic method Phylogenetics Phylogeny Plant Preparations - chemistry Plants, Medicinal - chemistry Plants, Medicinal - genetics Spacer Species species differentiation |
| title | Comparative Analysis of Genetic and Chemical Differences between Four Berberis Herbs Based on Molecular Phylogenetic and HPLC Methods |
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