Porcine-induced pluripotent stem cell-derived osteoblast-like cells ameliorate trabecular bone mass of osteoporotic rats
We created rat models of osteoporosis and verified a novel idea to recover bone mass via local cell transplantation. The rats were treated with ovariectomy, 0.1% calcium diet or 3 mg/kg body weight/day of prednisolone and porcine-induced pluripotent stem cell (piPSC)-derived osteoblast-like cells we...
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| Veröffentlicht in: | Regenerative medicine 2018-09, Vol.13 (6), p.659-671 |
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| creator | Liao, Yu-Jing Tang, Pin-Chi Lin, Chih-Hsun Chen, Lih-Ren Yang, Jenn-Rong |
| description | We created rat models of osteoporosis and verified a novel idea to recover bone mass via local cell transplantation.
The rats were treated with ovariectomy, 0.1% calcium diet or 3 mg/kg body weight/day of prednisolone and porcine-induced pluripotent stem cell (piPSC)-derived osteoblast-like cells were transplanted into the medullary cavity of the left femurs.
The piPSC-derived osteoblast-like cells exerted therapeutic potential on prednisolone treatment group, which confirmed by improvements in trabecular bone volume (15.93 ± 2.20%), bone surface/volume ratio (27.82 ± 1.40 1/mm), thickness (1.40 ± 0.01 mm), separation (0.99 ± 0.10 mm), number (1.13 ± 0.13 1/mm) and total porosity (84.06 ± 2.20%).
These results first uncovered therapeutic potential of xenotransplantation with piPSCs for glucocorticoid-induced osteoporosis treatment in the rat models. |
| doi_str_mv | 10.2217/rme-2018-0014 |
| format | Article |
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The rats were treated with ovariectomy, 0.1% calcium diet or 3 mg/kg body weight/day of prednisolone and porcine-induced pluripotent stem cell (piPSC)-derived osteoblast-like cells were transplanted into the medullary cavity of the left femurs.
The piPSC-derived osteoblast-like cells exerted therapeutic potential on prednisolone treatment group, which confirmed by improvements in trabecular bone volume (15.93 ± 2.20%), bone surface/volume ratio (27.82 ± 1.40 1/mm), thickness (1.40 ± 0.01 mm), separation (0.99 ± 0.10 mm), number (1.13 ± 0.13 1/mm) and total porosity (84.06 ± 2.20%).
These results first uncovered therapeutic potential of xenotransplantation with piPSCs for glucocorticoid-induced osteoporosis treatment in the rat models.</description><identifier>ISSN: 1746-0751</identifier><identifier>EISSN: 1746-076X</identifier><identifier>DOI: 10.2217/rme-2018-0014</identifier><identifier>PMID: 30256173</identifier><language>eng</language><publisher>England: Future Medicine Ltd</publisher><subject>Animals ; Biomarkers - metabolism ; Bone density ; Cancellous Bone - transplantation ; Cell Culture Techniques ; Cell Differentiation ; Diet ; Disease ; Disease Models, Animal ; femur ; Fibroblasts ; Fractures ; Immunoglobulins ; induced pluripotent stem cells ; Osteoporosis ; Osteoporosis - therapy ; Phenotype ; Pluripotent Stem Cells - transplantation ; prednisolone ; Rats ; Stem cells ; Swine ; trabecular bone mass</subject><ispartof>Regenerative medicine, 2018-09, Vol.13 (6), p.659-671</ispartof><rights>2018 Future Medicine Ltd</rights><rights>Copyright Future Medicine Ltd Sep 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c327t-2553b5dd41999b568c115dca29f49f09469927389bcd1302128b40e516438ee23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30256173$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liao, Yu-Jing</creatorcontrib><creatorcontrib>Tang, Pin-Chi</creatorcontrib><creatorcontrib>Lin, Chih-Hsun</creatorcontrib><creatorcontrib>Chen, Lih-Ren</creatorcontrib><creatorcontrib>Yang, Jenn-Rong</creatorcontrib><title>Porcine-induced pluripotent stem cell-derived osteoblast-like cells ameliorate trabecular bone mass of osteoporotic rats</title><title>Regenerative medicine</title><addtitle>Regen Med</addtitle><description>We created rat models of osteoporosis and verified a novel idea to recover bone mass via local cell transplantation.
The rats were treated with ovariectomy, 0.1% calcium diet or 3 mg/kg body weight/day of prednisolone and porcine-induced pluripotent stem cell (piPSC)-derived osteoblast-like cells were transplanted into the medullary cavity of the left femurs.
The piPSC-derived osteoblast-like cells exerted therapeutic potential on prednisolone treatment group, which confirmed by improvements in trabecular bone volume (15.93 ± 2.20%), bone surface/volume ratio (27.82 ± 1.40 1/mm), thickness (1.40 ± 0.01 mm), separation (0.99 ± 0.10 mm), number (1.13 ± 0.13 1/mm) and total porosity (84.06 ± 2.20%).
These results first uncovered therapeutic potential of xenotransplantation with piPSCs for glucocorticoid-induced osteoporosis treatment in the rat models.</description><subject>Animals</subject><subject>Biomarkers - metabolism</subject><subject>Bone density</subject><subject>Cancellous Bone - transplantation</subject><subject>Cell Culture Techniques</subject><subject>Cell Differentiation</subject><subject>Diet</subject><subject>Disease</subject><subject>Disease Models, Animal</subject><subject>femur</subject><subject>Fibroblasts</subject><subject>Fractures</subject><subject>Immunoglobulins</subject><subject>induced pluripotent stem cells</subject><subject>Osteoporosis</subject><subject>Osteoporosis - therapy</subject><subject>Phenotype</subject><subject>Pluripotent Stem Cells - transplantation</subject><subject>prednisolone</subject><subject>Rats</subject><subject>Stem cells</subject><subject>Swine</subject><subject>trabecular bone mass</subject><issn>1746-0751</issn><issn>1746-076X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kbtv0DAQhy0EoqUwsiJLLCwGnxM78YgqHpUq0aGV2CzHuUguSRz8QOW_xyGlA1InP-67n87-CHkN_L0Q0H2ICzLBoWecQ_uEnELXKsY79f3pw17CCXmR0i3nsumleE5OGi6kgq45JXdXITq_IvPrWByOdJtL9FvIuGaaMi7U4TyzEaP_VauhXoVhtimz2f_Av8VE7YKzD9FmpDnaAV2ZbaRDWJEuNiUapqNxCzFk72gl00vybLJzwlf36xm5-fzp-vwru_z25eL84yVzjegyE1I2gxzHFrTWg1S9A5Cjs0JPrZ64bpXWomt6PbgR6rNA9EPLUYJqmx5RNGfk3ZG7xfCzYMpm8Wkf264YSjICQCjgLYeKvv0PvQ0lrnU6U79aSS642gPZQbkYUoo4mS36xcbfBvjOdaYqMbsSsyup_Jv71DIsOD7Q_xxUQB_AVHKJmJzH1aE5TrXD734eCf8Dv3ebYw</recordid><startdate>20180901</startdate><enddate>20180901</enddate><creator>Liao, Yu-Jing</creator><creator>Tang, Pin-Chi</creator><creator>Lin, Chih-Hsun</creator><creator>Chen, Lih-Ren</creator><creator>Yang, Jenn-Rong</creator><general>Future Medicine Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>EHMNL</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope></search><sort><creationdate>20180901</creationdate><title>Porcine-induced pluripotent stem cell-derived osteoblast-like cells ameliorate trabecular bone mass of osteoporotic rats</title><author>Liao, Yu-Jing ; 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The rats were treated with ovariectomy, 0.1% calcium diet or 3 mg/kg body weight/day of prednisolone and porcine-induced pluripotent stem cell (piPSC)-derived osteoblast-like cells were transplanted into the medullary cavity of the left femurs.
The piPSC-derived osteoblast-like cells exerted therapeutic potential on prednisolone treatment group, which confirmed by improvements in trabecular bone volume (15.93 ± 2.20%), bone surface/volume ratio (27.82 ± 1.40 1/mm), thickness (1.40 ± 0.01 mm), separation (0.99 ± 0.10 mm), number (1.13 ± 0.13 1/mm) and total porosity (84.06 ± 2.20%).
These results first uncovered therapeutic potential of xenotransplantation with piPSCs for glucocorticoid-induced osteoporosis treatment in the rat models.</abstract><cop>England</cop><pub>Future Medicine Ltd</pub><pmid>30256173</pmid><doi>10.2217/rme-2018-0014</doi><tpages>13</tpages></addata></record> |
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| subjects | Animals Biomarkers - metabolism Bone density Cancellous Bone - transplantation Cell Culture Techniques Cell Differentiation Diet Disease Disease Models, Animal femur Fibroblasts Fractures Immunoglobulins induced pluripotent stem cells Osteoporosis Osteoporosis - therapy Phenotype Pluripotent Stem Cells - transplantation prednisolone Rats Stem cells Swine trabecular bone mass |
| title | Porcine-induced pluripotent stem cell-derived osteoblast-like cells ameliorate trabecular bone mass of osteoporotic rats |
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