Adenoviral and AAV-Mediated Gene Transfer to the Inner Ear: Role of Serotype, Promoter, and Viral Load on In Vivo and In Vitro Infection Efficiencies
The lack of effective treatments for many forms of hearing and vestibular disorders has produced interest in virally mediated gene therapies. However, to develop a gene therapy strategy that would successfully treat inner ear disorders, appropriate viral vectors capable of transfecting cochlear and...
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| creator | Luebke, Anne E. Rova, Cherokee Von Doersten, Peter G. Poulsen, David J. |
| description | The lack of effective treatments for many forms of hearing and vestibular disorders has produced interest in virally mediated gene therapies. However, to develop a gene therapy strategy that would successfully treat inner ear disorders, appropriate viral vectors capable of transfecting cochlear and support cells must be identified. While virally mediated gene transfer into the inner ear has been accomplished using herpes simplex type I virus, vaccinia virus, retroviruses, adenovirus, and adeno-associated virus (AAV), we will restrict our discussion to AAV and adenoviral vectors. Issues such as vector toxicity and load, viral serotype and backbone, and promoter specificity are discussed and contrasted for both in vivo vs. in vitro inner ear gene transfer. |
| doi_str_mv | 10.1159/000218209 |
| format | Book Chapter |
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Karger AG</publisher><subject>Adeno-associated virus ; Adenoviridae - genetics ; Adenovirus ; Animals ; Chapter ; Cochlea - metabolism ; Cytomegalovirus - genetics ; Dependovirus - genetics ; Gene Transfer Techniques ; Genetic Therapy - methods ; Humans ; Immunohistochemistry ; Organ Specificity ; Promoter Regions, Genetic - genetics ; Receptors, Virus - metabolism ; Retrovirus ; Serotyping ; Transgenes - genetics ; Vaccinia virus ; Viral Load ; Virus Replication</subject><ispartof>Advances in Oto-Rhino-Laryngology, 2009, Vol.66, p.87-98</ispartof><rights>2009 S. Karger AG, Basel</rights><rights>Copyright (c) 2009 S. 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However, to develop a gene therapy strategy that would successfully treat inner ear disorders, appropriate viral vectors capable of transfecting cochlear and support cells must be identified. While virally mediated gene transfer into the inner ear has been accomplished using herpes simplex type I virus, vaccinia virus, retroviruses, adenovirus, and adeno-associated virus (AAV), we will restrict our discussion to AAV and adenoviral vectors. Issues such as vector toxicity and load, viral serotype and backbone, and promoter specificity are discussed and contrasted for both in vivo vs. in vitro inner ear gene transfer.</abstract><cop>Basel, Switzerland</cop><pub>S. Karger AG</pub><pmid>19494574</pmid><doi>10.1159/000218209</doi><oclcid>923648169</oclcid><tpages>12</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0065-3071 |
| ispartof | Advances in Oto-Rhino-Laryngology, 2009, Vol.66, p.87-98 |
| issn | 0065-3071 1662-2847 |
| language | eng |
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| source | Karger eBooks Collection Archiv (DFG Nationallizenzen); MEDLINE; Karger Book Series |
| subjects | Adeno-associated virus Adenoviridae - genetics Adenovirus Animals Chapter Cochlea - metabolism Cytomegalovirus - genetics Dependovirus - genetics Gene Transfer Techniques Genetic Therapy - methods Humans Immunohistochemistry Organ Specificity Promoter Regions, Genetic - genetics Receptors, Virus - metabolism Retrovirus Serotyping Transgenes - genetics Vaccinia virus Viral Load Virus Replication |
| title | Adenoviral and AAV-Mediated Gene Transfer to the Inner Ear: Role of Serotype, Promoter, and Viral Load on In Vivo and In Vitro Infection Efficiencies |
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