The large GTPase Mx1 binds Kif5B for cargo transport along microtubules
A highly specific transport and sorting machinery directing secretory cargo to the apical or basolateral plasma membrane maintains the characteristic polarized architecture of epithelial cells. This machinery comprises a defined set of transport carriers, which are crucial for cargo delivery to the...
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Veröffentlicht in: | Traffic (Copenhagen, Denmark) Denmark), 2018-12, Vol.19 (12), p.947-964 |
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creator | Ringer, Karina Riehl, Jana Müller, Manuel Dewes, Jenny Hoff, Florian Jacob, Ralf |
description | A highly specific transport and sorting machinery directing secretory cargo to the apical or basolateral plasma membrane maintains the characteristic polarized architecture of epithelial cells. This machinery comprises a defined set of transport carriers, which are crucial for cargo delivery to the correct membrane domain. Each carrier is composed of a distinct set of proteins to verify precise routing and cargo selection. Among these components, the dynamin‐related GTPase Mx1 was identified on post‐Golgi vesicles destined for the apical membrane of MDCK cells. In addition to the presence on late secretory compartments, Mx1 was also detected on compartments of the early secretory pathway. Vesicular structures positive for this GTPase are highly dynamic, and we have studied the influence of the microtubule cytoskeleton on this motility. Live‐cell microscopy indicated that microtubule disruption using nocodazole inhibits long‐range trafficking of these structures. Mx1 directly or indirectly interacts with α‐tubulin and the kinesin motor Kif5B as assessed by coimmunoprecipitation. In agreement with these observations knock out of Mx1 or a mutation in the unstructured L4 loop of Mx1 decreases the efficiency of apical cargo delivery. Interestingly, the L4 loop mutant still interacts with Kif5B; however, it causes vesicle elongation. This suggests that Mx1 aids in vesicle fission and stabilizes the interaction between Kif5B, microtubules and apical transport carriers.
The GTPase Mx1 aids in vesicle fission and stabilizes the interaction of the kinesin motor Kif5B with apical transport vesicles. |
doi_str_mv | 10.1111/tra.12616 |
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The GTPase Mx1 aids in vesicle fission and stabilizes the interaction of the kinesin motor Kif5B with apical transport vesicles.</description><identifier>ISSN: 1398-9219</identifier><identifier>EISSN: 1600-0854</identifier><identifier>DOI: 10.1111/tra.12616</identifier><identifier>PMID: 30246279</identifier><language>eng</language><publisher>Former Munksgaard: John Wiley & Sons A/S</publisher><subject>apical transport ; cell polarity ; Cytoskeleton ; Dynamin ; Epithelial cells ; Golgi apparatus ; Guanosine triphosphatases ; Kinesin ; microtubule ; Microtubules ; Mx1 ; Myxovirus resistance proteins ; Nocodazole ; Tubulin</subject><ispartof>Traffic (Copenhagen, Denmark), 2018-12, Vol.19 (12), p.947-964</ispartof><rights>2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd</rights><rights>2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3886-3b9716204e0948c7485409ec14ce23bf37551e8910760347ca6bde1f29103e723</citedby><cites>FETCH-LOGICAL-c3886-3b9716204e0948c7485409ec14ce23bf37551e8910760347ca6bde1f29103e723</cites><orcidid>0000-0002-9251-6578</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Ftra.12616$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Ftra.12616$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,1434,27929,27930,45579,45580,46414,46838</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30246279$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ringer, Karina</creatorcontrib><creatorcontrib>Riehl, Jana</creatorcontrib><creatorcontrib>Müller, Manuel</creatorcontrib><creatorcontrib>Dewes, Jenny</creatorcontrib><creatorcontrib>Hoff, Florian</creatorcontrib><creatorcontrib>Jacob, Ralf</creatorcontrib><title>The large GTPase Mx1 binds Kif5B for cargo transport along microtubules</title><title>Traffic (Copenhagen, Denmark)</title><addtitle>Traffic</addtitle><description>A highly specific transport and sorting machinery directing secretory cargo to the apical or basolateral plasma membrane maintains the characteristic polarized architecture of epithelial cells. This machinery comprises a defined set of transport carriers, which are crucial for cargo delivery to the correct membrane domain. Each carrier is composed of a distinct set of proteins to verify precise routing and cargo selection. Among these components, the dynamin‐related GTPase Mx1 was identified on post‐Golgi vesicles destined for the apical membrane of MDCK cells. In addition to the presence on late secretory compartments, Mx1 was also detected on compartments of the early secretory pathway. Vesicular structures positive for this GTPase are highly dynamic, and we have studied the influence of the microtubule cytoskeleton on this motility. Live‐cell microscopy indicated that microtubule disruption using nocodazole inhibits long‐range trafficking of these structures. Mx1 directly or indirectly interacts with α‐tubulin and the kinesin motor Kif5B as assessed by coimmunoprecipitation. In agreement with these observations knock out of Mx1 or a mutation in the unstructured L4 loop of Mx1 decreases the efficiency of apical cargo delivery. Interestingly, the L4 loop mutant still interacts with Kif5B; however, it causes vesicle elongation. This suggests that Mx1 aids in vesicle fission and stabilizes the interaction between Kif5B, microtubules and apical transport carriers.
The GTPase Mx1 aids in vesicle fission and stabilizes the interaction of the kinesin motor Kif5B with apical transport vesicles.</description><subject>apical transport</subject><subject>cell polarity</subject><subject>Cytoskeleton</subject><subject>Dynamin</subject><subject>Epithelial cells</subject><subject>Golgi apparatus</subject><subject>Guanosine triphosphatases</subject><subject>Kinesin</subject><subject>microtubule</subject><subject>Microtubules</subject><subject>Mx1</subject><subject>Myxovirus resistance proteins</subject><subject>Nocodazole</subject><subject>Tubulin</subject><issn>1398-9219</issn><issn>1600-0854</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp1kMtOwzAQRS0EouWx4AeQJTawSOtXnHhZKiiIIhAKaytxJyVVHsVOBP17XFJYIOHNWKOjOzMHoTNKRtS_cWvTEWWSyj00pJKQgMSh2Pd_ruJAMaoG6Mi5FSGEhUIcogEnTEgWqSGaJW-Ay9QuAc-S59QBfvykOCvqhcMPRR5e47yx2HigwX5M7daNbXFaNvUSV4WxTdtlXQnuBB3kaengdFeP0evtTTK9C-ZPs_vpZB4YHscy4JmKqGREAFEiNpHwixIFhgoDjGc5j8KQQqwoiSThIjKpzBZAc-Y7HCLGj9Fln7u2zXsHrtVV4QyUZVpD0znNvI9ISBrGHr34g66aztZ-O09xH-59bKmrnvK3OGch12tbVKndaEr01q72Z-tvu5493yV2WQWLX_JHpwfGPfBRlLD5P0knL5M-8gsVUH_Z</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Ringer, Karina</creator><creator>Riehl, Jana</creator><creator>Müller, Manuel</creator><creator>Dewes, Jenny</creator><creator>Hoff, Florian</creator><creator>Jacob, Ralf</creator><general>John Wiley & Sons A/S</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9251-6578</orcidid></search><sort><creationdate>201812</creationdate><title>The large GTPase Mx1 binds Kif5B for cargo transport along microtubules</title><author>Ringer, Karina ; Riehl, Jana ; Müller, Manuel ; Dewes, Jenny ; Hoff, Florian ; Jacob, Ralf</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3886-3b9716204e0948c7485409ec14ce23bf37551e8910760347ca6bde1f29103e723</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>apical transport</topic><topic>cell polarity</topic><topic>Cytoskeleton</topic><topic>Dynamin</topic><topic>Epithelial cells</topic><topic>Golgi apparatus</topic><topic>Guanosine triphosphatases</topic><topic>Kinesin</topic><topic>microtubule</topic><topic>Microtubules</topic><topic>Mx1</topic><topic>Myxovirus resistance proteins</topic><topic>Nocodazole</topic><topic>Tubulin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ringer, Karina</creatorcontrib><creatorcontrib>Riehl, Jana</creatorcontrib><creatorcontrib>Müller, Manuel</creatorcontrib><creatorcontrib>Dewes, Jenny</creatorcontrib><creatorcontrib>Hoff, Florian</creatorcontrib><creatorcontrib>Jacob, Ralf</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Traffic (Copenhagen, Denmark)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ringer, Karina</au><au>Riehl, Jana</au><au>Müller, Manuel</au><au>Dewes, Jenny</au><au>Hoff, Florian</au><au>Jacob, Ralf</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The large GTPase Mx1 binds Kif5B for cargo transport along microtubules</atitle><jtitle>Traffic (Copenhagen, Denmark)</jtitle><addtitle>Traffic</addtitle><date>2018-12</date><risdate>2018</risdate><volume>19</volume><issue>12</issue><spage>947</spage><epage>964</epage><pages>947-964</pages><issn>1398-9219</issn><eissn>1600-0854</eissn><abstract>A highly specific transport and sorting machinery directing secretory cargo to the apical or basolateral plasma membrane maintains the characteristic polarized architecture of epithelial cells. This machinery comprises a defined set of transport carriers, which are crucial for cargo delivery to the correct membrane domain. Each carrier is composed of a distinct set of proteins to verify precise routing and cargo selection. Among these components, the dynamin‐related GTPase Mx1 was identified on post‐Golgi vesicles destined for the apical membrane of MDCK cells. In addition to the presence on late secretory compartments, Mx1 was also detected on compartments of the early secretory pathway. Vesicular structures positive for this GTPase are highly dynamic, and we have studied the influence of the microtubule cytoskeleton on this motility. Live‐cell microscopy indicated that microtubule disruption using nocodazole inhibits long‐range trafficking of these structures. Mx1 directly or indirectly interacts with α‐tubulin and the kinesin motor Kif5B as assessed by coimmunoprecipitation. In agreement with these observations knock out of Mx1 or a mutation in the unstructured L4 loop of Mx1 decreases the efficiency of apical cargo delivery. Interestingly, the L4 loop mutant still interacts with Kif5B; however, it causes vesicle elongation. This suggests that Mx1 aids in vesicle fission and stabilizes the interaction between Kif5B, microtubules and apical transport carriers.
The GTPase Mx1 aids in vesicle fission and stabilizes the interaction of the kinesin motor Kif5B with apical transport vesicles.</abstract><cop>Former Munksgaard</cop><pub>John Wiley & Sons A/S</pub><pmid>30246279</pmid><doi>10.1111/tra.12616</doi><tpages>19</tpages><orcidid>https://orcid.org/0000-0002-9251-6578</orcidid><oa>free_for_read</oa></addata></record> |
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source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Wiley Online Library (Open Access Collection) |
subjects | apical transport cell polarity Cytoskeleton Dynamin Epithelial cells Golgi apparatus Guanosine triphosphatases Kinesin microtubule Microtubules Mx1 Myxovirus resistance proteins Nocodazole Tubulin |
title | The large GTPase Mx1 binds Kif5B for cargo transport along microtubules |
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