Identification of Carassius auratus gibelio liver cell proteins interacting with the GABAA receptor γ2 subunit using a yeast two-hybrid system
The γ-aminobutyric acid type A (GABA A ) receptor is an important pentameric inhibitory neurotransmitter receptor, and the γ2 subunit of this receptor plays a key role in potentiation of the GABA A response. We previously detected that the expression of GABA A receptor in the livers of Carassius aur...
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creator | Ma, Rong-Rong Sun, Jing Fang, Wen-Hong Dong, Ya-Ping Ruan, Ji-Ming Yang, Xian-Le Hu, Kun |
description | The γ-aminobutyric acid type A (GABA
A
) receptor is an important pentameric inhibitory neurotransmitter receptor, and the γ2 subunit of this receptor plays a key role in potentiation of the GABA
A
response. We previously detected that the expression of GABA
A
receptor in the livers of
Carassius auratus gibelio
significantly increased after medication (avermectin and difloxacin treatment). In order to better understand the mechanism of action of the GABA
A
receptor γ2 subunit in the livers of
C. auratus gibelio
, we constructed a
C. auratus gibelio
liver cDNA library (the titer value of 1.2 × 10
6
cfu/mL) and identified the proteins that interact with the GABA
A
receptor γ2 subunit by using a yeast two-hybrid assay. The yeast two-hybrid screening yielded seven positive clones, namely, prelid3b, cdc42, sgk1, spg21, proteasome, chia.5, and AP-3 complex subunit beta-1, all of which have been annotated by the NCBI database. The functions of these proteins are complex; therefore, additional studies are required to determine the specific interactions of these proteins with the GABA
A
receptor γ2 subunit in the liver of
C. auratus gibelio
. Although the interactions identified by the yeast two-hybrid system should be considered as preliminary results, the findings of this study may provide further direction and a foundation for future research focusing on the mechanisms of the GABA
A
receptor γ2 subunit in
C. auratus gibelio
livers. |
doi_str_mv | 10.1007/s10695-018-0554-5 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2111142115</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2111142115</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1945-5b2c5b13302d3097ebb9081ebc383c548960036dac254b3c067a4013e9a694c13</originalsourceid><addsrcrecordid>eNp1kc2KVDEQhYMo2I4-gLuAGzdxKsnN_Vm2jTMODLjR9SVJV3dnuJ20qVyHfgofxvfwmcylBWHAWlRtvnOow2HsrYQPEqC7JgntYATIXoAxjTDP2EqaTgsj2_45W8GgQMiuUS_ZK6IHABi6Vq7Yz7stxhJ2wdsSUuRpxzc2W6IwE7dztqXefXA4hcSn8AMz9zhN_JRTwRCJh1gwW19C3PPHUA68HJDfrj-u1zyjx1NJmf_-pTjNbo6h8JkW0vIzWiq8PCZxOLsctpzOVPD4mr3Y2Ynwzd97xb7dfPq6-Szuv9zebdb3wsuhMcI45Y2TWoPa6hoFnRugl-i87rU3TT-0ALrdWq9M47SHtrMNSI2DbYfGS33F3l98a5DvM1IZj4GWZDZimmlUsk5Tt6nouyfoQ5pzrN8tFOhBK91VSl4onxNRxt14yuFo83mUMC4VjZeKxlrRuFQ0Ls7qoqHKxj3mf87_F_0BiqqVKQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2110393237</pqid></control><display><type>article</type><title>Identification of Carassius auratus gibelio liver cell proteins interacting with the GABAA receptor γ2 subunit using a yeast two-hybrid system</title><source>SpringerLink Journals - AutoHoldings</source><creator>Ma, Rong-Rong ; Sun, Jing ; Fang, Wen-Hong ; Dong, Ya-Ping ; Ruan, Ji-Ming ; Yang, Xian-Le ; Hu, Kun</creator><creatorcontrib>Ma, Rong-Rong ; Sun, Jing ; Fang, Wen-Hong ; Dong, Ya-Ping ; Ruan, Ji-Ming ; Yang, Xian-Le ; Hu, Kun</creatorcontrib><description>The γ-aminobutyric acid type A (GABA
A
) receptor is an important pentameric inhibitory neurotransmitter receptor, and the γ2 subunit of this receptor plays a key role in potentiation of the GABA
A
response. We previously detected that the expression of GABA
A
receptor in the livers of
Carassius auratus gibelio
significantly increased after medication (avermectin and difloxacin treatment). In order to better understand the mechanism of action of the GABA
A
receptor γ2 subunit in the livers of
C. auratus gibelio
, we constructed a
C. auratus gibelio
liver cDNA library (the titer value of 1.2 × 10
6
cfu/mL) and identified the proteins that interact with the GABA
A
receptor γ2 subunit by using a yeast two-hybrid assay. The yeast two-hybrid screening yielded seven positive clones, namely, prelid3b, cdc42, sgk1, spg21, proteasome, chia.5, and AP-3 complex subunit beta-1, all of which have been annotated by the NCBI database. The functions of these proteins are complex; therefore, additional studies are required to determine the specific interactions of these proteins with the GABA
A
receptor γ2 subunit in the liver of
C. auratus gibelio
. Although the interactions identified by the yeast two-hybrid system should be considered as preliminary results, the findings of this study may provide further direction and a foundation for future research focusing on the mechanisms of the GABA
A
receptor γ2 subunit in
C. auratus gibelio
livers.</description><identifier>ISSN: 0920-1742</identifier><identifier>EISSN: 1573-5168</identifier><identifier>DOI: 10.1007/s10695-018-0554-5</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Animal Physiology ; Avermectin ; Biomedical and Life Sciences ; Carassius auratus gibelio ; Cdc42 protein ; Clones ; Complementary DNA ; Difloxacin ; DNA ; Freshwater & Marine Ecology ; Hepatocytes ; Histology ; Hybrid systems ; Identification ; Interactions ; Life Sciences ; Liver ; Morphology ; Neurotransmitters ; Potentiation ; Proteasomes ; Proteins ; Yeast ; Yeasts ; Zoology ; γ-Aminobutyric acid A receptors</subject><ispartof>Fish physiology and biochemistry, 2019-02, Vol.45 (1), p.199-208</ispartof><rights>Springer Nature B.V. 2018</rights><rights>Fish Physiology and Biochemistry is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1945-5b2c5b13302d3097ebb9081ebc383c548960036dac254b3c067a4013e9a694c13</citedby><cites>FETCH-LOGICAL-c1945-5b2c5b13302d3097ebb9081ebc383c548960036dac254b3c067a4013e9a694c13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10695-018-0554-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10695-018-0554-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,777,781,27905,27906,41469,42538,51300</link.rule.ids></links><search><creatorcontrib>Ma, Rong-Rong</creatorcontrib><creatorcontrib>Sun, Jing</creatorcontrib><creatorcontrib>Fang, Wen-Hong</creatorcontrib><creatorcontrib>Dong, Ya-Ping</creatorcontrib><creatorcontrib>Ruan, Ji-Ming</creatorcontrib><creatorcontrib>Yang, Xian-Le</creatorcontrib><creatorcontrib>Hu, Kun</creatorcontrib><title>Identification of Carassius auratus gibelio liver cell proteins interacting with the GABAA receptor γ2 subunit using a yeast two-hybrid system</title><title>Fish physiology and biochemistry</title><addtitle>Fish Physiol Biochem</addtitle><description>The γ-aminobutyric acid type A (GABA
A
) receptor is an important pentameric inhibitory neurotransmitter receptor, and the γ2 subunit of this receptor plays a key role in potentiation of the GABA
A
response. We previously detected that the expression of GABA
A
receptor in the livers of
Carassius auratus gibelio
significantly increased after medication (avermectin and difloxacin treatment). In order to better understand the mechanism of action of the GABA
A
receptor γ2 subunit in the livers of
C. auratus gibelio
, we constructed a
C. auratus gibelio
liver cDNA library (the titer value of 1.2 × 10
6
cfu/mL) and identified the proteins that interact with the GABA
A
receptor γ2 subunit by using a yeast two-hybrid assay. The yeast two-hybrid screening yielded seven positive clones, namely, prelid3b, cdc42, sgk1, spg21, proteasome, chia.5, and AP-3 complex subunit beta-1, all of which have been annotated by the NCBI database. The functions of these proteins are complex; therefore, additional studies are required to determine the specific interactions of these proteins with the GABA
A
receptor γ2 subunit in the liver of
C. auratus gibelio
. Although the interactions identified by the yeast two-hybrid system should be considered as preliminary results, the findings of this study may provide further direction and a foundation for future research focusing on the mechanisms of the GABA
A
receptor γ2 subunit in
C. auratus gibelio
livers.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Animal Physiology</subject><subject>Avermectin</subject><subject>Biomedical and Life Sciences</subject><subject>Carassius auratus gibelio</subject><subject>Cdc42 protein</subject><subject>Clones</subject><subject>Complementary DNA</subject><subject>Difloxacin</subject><subject>DNA</subject><subject>Freshwater & Marine Ecology</subject><subject>Hepatocytes</subject><subject>Histology</subject><subject>Hybrid systems</subject><subject>Identification</subject><subject>Interactions</subject><subject>Life Sciences</subject><subject>Liver</subject><subject>Morphology</subject><subject>Neurotransmitters</subject><subject>Potentiation</subject><subject>Proteasomes</subject><subject>Proteins</subject><subject>Yeast</subject><subject>Yeasts</subject><subject>Zoology</subject><subject>γ-Aminobutyric acid A 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of Carassius auratus gibelio liver cell proteins interacting with the GABAA receptor γ2 subunit using a yeast two-hybrid system</title><author>Ma, Rong-Rong ; Sun, Jing ; Fang, Wen-Hong ; Dong, Ya-Ping ; Ruan, Ji-Ming ; Yang, Xian-Le ; Hu, Kun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1945-5b2c5b13302d3097ebb9081ebc383c548960036dac254b3c067a4013e9a694c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Animal Physiology</topic><topic>Avermectin</topic><topic>Biomedical and Life Sciences</topic><topic>Carassius auratus gibelio</topic><topic>Cdc42 protein</topic><topic>Clones</topic><topic>Complementary DNA</topic><topic>Difloxacin</topic><topic>DNA</topic><topic>Freshwater & Marine Ecology</topic><topic>Hepatocytes</topic><topic>Histology</topic><topic>Hybrid systems</topic><topic>Identification</topic><topic>Interactions</topic><topic>Life Sciences</topic><topic>Liver</topic><topic>Morphology</topic><topic>Neurotransmitters</topic><topic>Potentiation</topic><topic>Proteasomes</topic><topic>Proteins</topic><topic>Yeast</topic><topic>Yeasts</topic><topic>Zoology</topic><topic>γ-Aminobutyric acid A receptors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ma, Rong-Rong</creatorcontrib><creatorcontrib>Sun, Jing</creatorcontrib><creatorcontrib>Fang, Wen-Hong</creatorcontrib><creatorcontrib>Dong, Ya-Ping</creatorcontrib><creatorcontrib>Ruan, Ji-Ming</creatorcontrib><creatorcontrib>Yang, Xian-Le</creatorcontrib><creatorcontrib>Hu, Kun</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Aqualine</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences 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biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ma, Rong-Rong</au><au>Sun, Jing</au><au>Fang, Wen-Hong</au><au>Dong, Ya-Ping</au><au>Ruan, Ji-Ming</au><au>Yang, Xian-Le</au><au>Hu, Kun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Carassius auratus gibelio liver cell proteins interacting with the GABAA receptor γ2 subunit using a yeast two-hybrid system</atitle><jtitle>Fish physiology and biochemistry</jtitle><stitle>Fish Physiol Biochem</stitle><date>2019-02-01</date><risdate>2019</risdate><volume>45</volume><issue>1</issue><spage>199</spage><epage>208</epage><pages>199-208</pages><issn>0920-1742</issn><eissn>1573-5168</eissn><abstract>The γ-aminobutyric acid type A (GABA
A
) receptor is an important pentameric inhibitory neurotransmitter receptor, and the γ2 subunit of this receptor plays a key role in potentiation of the GABA
A
response. We previously detected that the expression of GABA
A
receptor in the livers of
Carassius auratus gibelio
significantly increased after medication (avermectin and difloxacin treatment). In order to better understand the mechanism of action of the GABA
A
receptor γ2 subunit in the livers of
C. auratus gibelio
, we constructed a
C. auratus gibelio
liver cDNA library (the titer value of 1.2 × 10
6
cfu/mL) and identified the proteins that interact with the GABA
A
receptor γ2 subunit by using a yeast two-hybrid assay. The yeast two-hybrid screening yielded seven positive clones, namely, prelid3b, cdc42, sgk1, spg21, proteasome, chia.5, and AP-3 complex subunit beta-1, all of which have been annotated by the NCBI database. The functions of these proteins are complex; therefore, additional studies are required to determine the specific interactions of these proteins with the GABA
A
receptor γ2 subunit in the liver of
C. auratus gibelio
. Although the interactions identified by the yeast two-hybrid system should be considered as preliminary results, the findings of this study may provide further direction and a foundation for future research focusing on the mechanisms of the GABA
A
receptor γ2 subunit in
C. auratus gibelio
livers.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><doi>10.1007/s10695-018-0554-5</doi><tpages>10</tpages></addata></record> |
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source | SpringerLink Journals - AutoHoldings |
subjects | Animal Anatomy Animal Biochemistry Animal Physiology Avermectin Biomedical and Life Sciences Carassius auratus gibelio Cdc42 protein Clones Complementary DNA Difloxacin DNA Freshwater & Marine Ecology Hepatocytes Histology Hybrid systems Identification Interactions Life Sciences Liver Morphology Neurotransmitters Potentiation Proteasomes Proteins Yeast Yeasts Zoology γ-Aminobutyric acid A receptors |
title | Identification of Carassius auratus gibelio liver cell proteins interacting with the GABAA receptor γ2 subunit using a yeast two-hybrid system |
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