Diazinon oxon interferes with differentiation of rat C6 glioma cells
The purpose of this study was to evaluate the toxicity of diazinon oxon (DZO), a major in vivo metabolite of the organophosphate insecticide diazinon (DZ), on differentiating rat C6 glioma cells. At concentrations shown to be non-cytotoxic by both the MTT and the Kenacid blue dye binding assays (1,...
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| Veröffentlicht in: | Toxicology in vitro 2009-12, Vol.23 (8), p.1548-1552 |
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| container_title | Toxicology in vitro |
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| creator | Sidiropoulou, E. Sachana, M. Flaskos, J. Harris, W. Hargreaves, A.J. Woldehiwet, Z. |
| description | The purpose of this study was to evaluate the toxicity of diazinon oxon (DZO), a major in vivo metabolite of the organophosphate insecticide diazinon (DZ), on differentiating rat C6 glioma cells. At concentrations shown to be non-cytotoxic by both the MTT and the Kenacid blue dye binding assays (1, 5 and 10
μM), DZO caused after 24
h a reduction in the number of extensions developed from C6 cells induced to differentiate by serum withdrawal and addition of sodium butyrate. Densitometric scanning of Western blots of extracts of C6 cells demonstrated that, at all concentrations used, DZO decreased after 24
h the expression of glial fibrillary acidic protein (GFAP) compared to controls. In addition, exposure to 10
μM DZO for 24
h reduced the levels of tubulin and microtubule associated protein 1B (MAP1B). On the other hand, levels of MAP2c were not affected by DZO treatment. In contrast to our previous data on DZ, the above findings suggest that its oxon metabolite, DZO, may, at biologically relevant, subcytotoxic concentrations, interfere with glial cell differentiation. |
| doi_str_mv | 10.1016/j.tiv.2009.07.005 |
| format | Article |
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μM), DZO caused after 24
h a reduction in the number of extensions developed from C6 cells induced to differentiate by serum withdrawal and addition of sodium butyrate. Densitometric scanning of Western blots of extracts of C6 cells demonstrated that, at all concentrations used, DZO decreased after 24
h the expression of glial fibrillary acidic protein (GFAP) compared to controls. In addition, exposure to 10
μM DZO for 24
h reduced the levels of tubulin and microtubule associated protein 1B (MAP1B). On the other hand, levels of MAP2c were not affected by DZO treatment. In contrast to our previous data on DZ, the above findings suggest that its oxon metabolite, DZO, may, at biologically relevant, subcytotoxic concentrations, interfere with glial cell differentiation.</description><identifier>ISSN: 0887-2333</identifier><identifier>EISSN: 1879-3177</identifier><identifier>DOI: 10.1016/j.tiv.2009.07.005</identifier><identifier>PMID: 19595759</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; C6 cells ; Cell Differentiation - drug effects ; Cell Line, Tumor ; Cholinesterase Inhibitors - toxicity ; Diazinon - metabolism ; Diazinon - toxicity ; Diazinon oxon ; Differentiation ; Dose-Response Relationship, Drug ; GFAP ; Glioma - pathology ; Insecticides - toxicity ; Microtubule proteins ; Rats</subject><ispartof>Toxicology in vitro, 2009-12, Vol.23 (8), p.1548-1552</ispartof><rights>2009 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c383t-e4a4b278b313e4c9baf0e70a35acfeaf208e5f2634564dd3de4e538d0718b1483</citedby><cites>FETCH-LOGICAL-c383t-e4a4b278b313e4c9baf0e70a35acfeaf208e5f2634564dd3de4e538d0718b1483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0887233309001799$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19595759$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sidiropoulou, E.</creatorcontrib><creatorcontrib>Sachana, M.</creatorcontrib><creatorcontrib>Flaskos, J.</creatorcontrib><creatorcontrib>Harris, W.</creatorcontrib><creatorcontrib>Hargreaves, A.J.</creatorcontrib><creatorcontrib>Woldehiwet, Z.</creatorcontrib><title>Diazinon oxon interferes with differentiation of rat C6 glioma cells</title><title>Toxicology in vitro</title><addtitle>Toxicol In Vitro</addtitle><description>The purpose of this study was to evaluate the toxicity of diazinon oxon (DZO), a major in vivo metabolite of the organophosphate insecticide diazinon (DZ), on differentiating rat C6 glioma cells. At concentrations shown to be non-cytotoxic by both the MTT and the Kenacid blue dye binding assays (1, 5 and 10
μM), DZO caused after 24
h a reduction in the number of extensions developed from C6 cells induced to differentiate by serum withdrawal and addition of sodium butyrate. Densitometric scanning of Western blots of extracts of C6 cells demonstrated that, at all concentrations used, DZO decreased after 24
h the expression of glial fibrillary acidic protein (GFAP) compared to controls. In addition, exposure to 10
μM DZO for 24
h reduced the levels of tubulin and microtubule associated protein 1B (MAP1B). On the other hand, levels of MAP2c were not affected by DZO treatment. In contrast to our previous data on DZ, the above findings suggest that its oxon metabolite, DZO, may, at biologically relevant, subcytotoxic concentrations, interfere with glial cell differentiation.</description><subject>Animals</subject><subject>C6 cells</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cholinesterase Inhibitors - toxicity</subject><subject>Diazinon - metabolism</subject><subject>Diazinon - toxicity</subject><subject>Diazinon oxon</subject><subject>Differentiation</subject><subject>Dose-Response Relationship, Drug</subject><subject>GFAP</subject><subject>Glioma - pathology</subject><subject>Insecticides - toxicity</subject><subject>Microtubule proteins</subject><subject>Rats</subject><issn>0887-2333</issn><issn>1879-3177</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1PwzAMhiMEYmPwA7ignri1OE3bpOKENr6kSVzgHKWpA5m6diQZX7-eVJvEjYstS49f2Q8h5xQyCrS6WmXBfmQ5QJ0BzwDKAzKlgtcpo5wfkikIwdOcMTYhJ96vIBIih2MyoXVZl7ysp2SxsOrH9kOfDF-x2D6gM-jQJ582vCWtNePUB6uCHSGTOBWSeZW8dnZYq0Rj1_lTcmRU5_Fs32fk5e72ef6QLp_uH-c3y1QzwUKKhSqanIuGUYaFrhtlADkoViptUJkcBJYmr1hRVkXbshYLLJlogVPR0EKwGbnc5W7c8L5FH-Ta-vEC1eOw9TKnFCrOqwjSHajd4L1DIzfOrpX7lhTkqE6uZFQnR3USuIxi4s7FPnzbrLH929i7isD1DsD44odFJ7222GtsrUMdZDvYf-J_AVc8fy0</recordid><startdate>20091201</startdate><enddate>20091201</enddate><creator>Sidiropoulou, E.</creator><creator>Sachana, M.</creator><creator>Flaskos, J.</creator><creator>Harris, W.</creator><creator>Hargreaves, A.J.</creator><creator>Woldehiwet, Z.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20091201</creationdate><title>Diazinon oxon interferes with differentiation of rat C6 glioma cells</title><author>Sidiropoulou, E. ; Sachana, M. ; Flaskos, J. ; Harris, W. ; Hargreaves, A.J. ; Woldehiwet, Z.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c383t-e4a4b278b313e4c9baf0e70a35acfeaf208e5f2634564dd3de4e538d0718b1483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>C6 cells</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cholinesterase Inhibitors - toxicity</topic><topic>Diazinon - metabolism</topic><topic>Diazinon - toxicity</topic><topic>Diazinon oxon</topic><topic>Differentiation</topic><topic>Dose-Response Relationship, Drug</topic><topic>GFAP</topic><topic>Glioma - pathology</topic><topic>Insecticides - toxicity</topic><topic>Microtubule proteins</topic><topic>Rats</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sidiropoulou, E.</creatorcontrib><creatorcontrib>Sachana, M.</creatorcontrib><creatorcontrib>Flaskos, J.</creatorcontrib><creatorcontrib>Harris, W.</creatorcontrib><creatorcontrib>Hargreaves, A.J.</creatorcontrib><creatorcontrib>Woldehiwet, Z.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Toxicology in vitro</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sidiropoulou, E.</au><au>Sachana, M.</au><au>Flaskos, J.</au><au>Harris, W.</au><au>Hargreaves, A.J.</au><au>Woldehiwet, Z.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diazinon oxon interferes with differentiation of rat C6 glioma cells</atitle><jtitle>Toxicology in vitro</jtitle><addtitle>Toxicol In Vitro</addtitle><date>2009-12-01</date><risdate>2009</risdate><volume>23</volume><issue>8</issue><spage>1548</spage><epage>1552</epage><pages>1548-1552</pages><issn>0887-2333</issn><eissn>1879-3177</eissn><abstract>The purpose of this study was to evaluate the toxicity of diazinon oxon (DZO), a major in vivo metabolite of the organophosphate insecticide diazinon (DZ), on differentiating rat C6 glioma cells. At concentrations shown to be non-cytotoxic by both the MTT and the Kenacid blue dye binding assays (1, 5 and 10
μM), DZO caused after 24
h a reduction in the number of extensions developed from C6 cells induced to differentiate by serum withdrawal and addition of sodium butyrate. Densitometric scanning of Western blots of extracts of C6 cells demonstrated that, at all concentrations used, DZO decreased after 24
h the expression of glial fibrillary acidic protein (GFAP) compared to controls. In addition, exposure to 10
μM DZO for 24
h reduced the levels of tubulin and microtubule associated protein 1B (MAP1B). On the other hand, levels of MAP2c were not affected by DZO treatment. In contrast to our previous data on DZ, the above findings suggest that its oxon metabolite, DZO, may, at biologically relevant, subcytotoxic concentrations, interfere with glial cell differentiation.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>19595759</pmid><doi>10.1016/j.tiv.2009.07.005</doi><tpages>5</tpages></addata></record> |
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| ispartof | Toxicology in vitro, 2009-12, Vol.23 (8), p.1548-1552 |
| issn | 0887-2333 1879-3177 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals C6 cells Cell Differentiation - drug effects Cell Line, Tumor Cholinesterase Inhibitors - toxicity Diazinon - metabolism Diazinon - toxicity Diazinon oxon Differentiation Dose-Response Relationship, Drug GFAP Glioma - pathology Insecticides - toxicity Microtubule proteins Rats |
| title | Diazinon oxon interferes with differentiation of rat C6 glioma cells |
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