Histochemical Demonstration of Calcium Accumulation in Muscle Fibres after Experimental Organophosphate Poisoning
The LD50 of subcutaneously-injected sarin (GB: isopropyl methylphosphonofluoridate) in mice was 172 μg kg-1. Mice were treated with sarin at doses between 25 and 150 μg kg-1, administered subcutaneously. After sacrifice of the animals, the diaphragms were removed and stained for acetylcholinesterase...
Gespeichert in:
| Veröffentlicht in: | Human & experimental toxicology 1990-07, Vol.9 (4), p.245-250 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 250 |
|---|---|
| container_issue | 4 |
| container_start_page | 245 |
| container_title | Human & experimental toxicology |
| container_volume | 9 |
| creator | Inns, R.H. Tuckwell, N.J. Bright, J.E. Marrs, T.C. |
| description | The LD50 of subcutaneously-injected sarin (GB: isopropyl methylphosphonofluoridate) in mice was 172 μg kg-1. Mice were treated with sarin at doses between 25 and 150 μg kg-1, administered subcutaneously. After sacrifice of the animals, the diaphragms were removed and stained for acetylcholinesterase activity and the presence of ionized calcium. Calcium was found in the diaphragms of those mice to which sarin had been administered at doses of 50 μg kg-1 or above. Calcium accumulation was not present in diaphragms from those animals that had received 25 μg kg-1. Calcium accumulation occurred earliest and remained longest in diaphragms from those animals receiving the highest doses. Accumulation of calcium was associated with end-plates, as demonstrated by an acetylcholinesterase histochemical method. |
| doi_str_mv | 10.1177/096032719000900407 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_21097900</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sage_id>10.1177_096032719000900407</sage_id><sourcerecordid>21097900</sourcerecordid><originalsourceid>FETCH-LOGICAL-c369t-f92711b9100cebbf381d803a6fe4b97478eb3c8857bf40daee3210d0dc0158713</originalsourceid><addsrcrecordid>eNp9kMFLwzAUxoMoc07_AUHIyVtd0nRNexxzc8JkHvRc0vR1y2iTLklB_3szOrwIHh7h5f3ex_s-hO4peaKU8ynJU8JiTnNCSKiE8As0pgnnUWjZJRqfgOhEXKMb5w4BS_MZHaFRzAIQ0zE6rpXzRu6hVVI0-Blao523wiujsanxQjRS9S2eS9m3fTP8K43feicbwCtVWnBY1B4sXn51YFUL2gelrd0Jbbq9cd1eeMDvRjmjld7doqtaNA7uzu8Efa6WH4t1tNm-vC7mm0iyNPdRnYezaZlTQiSUZc0yWmWEibSGpMx5wjMomcyyGS_rhFQCIPghFakkobOMUzZBj4NuZ82xB-eLVjkJTSM0mN4Vgc55SC2A8QBKa5yzUBddcCHsd0FJccq5-JtzWHo4q_dlC9XvyjnYMJ8Ocyd2UBxMb3Uw-5_iD5fNhzw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21097900</pqid></control><display><type>article</type><title>Histochemical Demonstration of Calcium Accumulation in Muscle Fibres after Experimental Organophosphate Poisoning</title><source>Access via SAGE</source><source>MEDLINE</source><source>Alma/SFX Local Collection</source><creator>Inns, R.H. ; Tuckwell, N.J. ; Bright, J.E. ; Marrs, T.C.</creator><creatorcontrib>Inns, R.H. ; Tuckwell, N.J. ; Bright, J.E. ; Marrs, T.C.</creatorcontrib><description>The LD50 of subcutaneously-injected sarin (GB: isopropyl methylphosphonofluoridate) in mice was 172 μg kg-1. Mice were treated with sarin at doses between 25 and 150 μg kg-1, administered subcutaneously. After sacrifice of the animals, the diaphragms were removed and stained for acetylcholinesterase activity and the presence of ionized calcium. Calcium was found in the diaphragms of those mice to which sarin had been administered at doses of 50 μg kg-1 or above. Calcium accumulation was not present in diaphragms from those animals that had received 25 μg kg-1. Calcium accumulation occurred earliest and remained longest in diaphragms from those animals receiving the highest doses. Accumulation of calcium was associated with end-plates, as demonstrated by an acetylcholinesterase histochemical method.</description><identifier>ISSN: 0960-3271</identifier><identifier>EISSN: 1477-0903</identifier><identifier>DOI: 10.1177/096032719000900407</identifier><identifier>PMID: 2390321</identifier><language>eng</language><publisher>Thousand Oaks, CA: SAGE Publications</publisher><subject>Acetylcholinesterase - metabolism ; Animals ; Calcium - metabolism ; Diaphragm - enzymology ; Histocytochemistry ; Lethal Dose 50 ; Male ; Mice ; Muscles - metabolism ; Muscles - pathology ; Organophosphate Poisoning ; Sarin - poisoning ; Sarin - toxicity</subject><ispartof>Human & experimental toxicology, 1990-07, Vol.9 (4), p.245-250</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c369t-f92711b9100cebbf381d803a6fe4b97478eb3c8857bf40daee3210d0dc0158713</citedby><cites>FETCH-LOGICAL-c369t-f92711b9100cebbf381d803a6fe4b97478eb3c8857bf40daee3210d0dc0158713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/096032719000900407$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/096032719000900407$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,780,784,21819,27924,27925,43621,43622</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2390321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Inns, R.H.</creatorcontrib><creatorcontrib>Tuckwell, N.J.</creatorcontrib><creatorcontrib>Bright, J.E.</creatorcontrib><creatorcontrib>Marrs, T.C.</creatorcontrib><title>Histochemical Demonstration of Calcium Accumulation in Muscle Fibres after Experimental Organophosphate Poisoning</title><title>Human & experimental toxicology</title><addtitle>Hum Exp Toxicol</addtitle><description>The LD50 of subcutaneously-injected sarin (GB: isopropyl methylphosphonofluoridate) in mice was 172 μg kg-1. Mice were treated with sarin at doses between 25 and 150 μg kg-1, administered subcutaneously. After sacrifice of the animals, the diaphragms were removed and stained for acetylcholinesterase activity and the presence of ionized calcium. Calcium was found in the diaphragms of those mice to which sarin had been administered at doses of 50 μg kg-1 or above. Calcium accumulation was not present in diaphragms from those animals that had received 25 μg kg-1. Calcium accumulation occurred earliest and remained longest in diaphragms from those animals receiving the highest doses. Accumulation of calcium was associated with end-plates, as demonstrated by an acetylcholinesterase histochemical method.</description><subject>Acetylcholinesterase - metabolism</subject><subject>Animals</subject><subject>Calcium - metabolism</subject><subject>Diaphragm - enzymology</subject><subject>Histocytochemistry</subject><subject>Lethal Dose 50</subject><subject>Male</subject><subject>Mice</subject><subject>Muscles - metabolism</subject><subject>Muscles - pathology</subject><subject>Organophosphate Poisoning</subject><subject>Sarin - poisoning</subject><subject>Sarin - toxicity</subject><issn>0960-3271</issn><issn>1477-0903</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFLwzAUxoMoc07_AUHIyVtd0nRNexxzc8JkHvRc0vR1y2iTLklB_3szOrwIHh7h5f3ex_s-hO4peaKU8ynJU8JiTnNCSKiE8As0pgnnUWjZJRqfgOhEXKMb5w4BS_MZHaFRzAIQ0zE6rpXzRu6hVVI0-Blao523wiujsanxQjRS9S2eS9m3fTP8K43feicbwCtVWnBY1B4sXn51YFUL2gelrd0Jbbq9cd1eeMDvRjmjld7doqtaNA7uzu8Efa6WH4t1tNm-vC7mm0iyNPdRnYezaZlTQiSUZc0yWmWEibSGpMx5wjMomcyyGS_rhFQCIPghFakkobOMUzZBj4NuZ82xB-eLVjkJTSM0mN4Vgc55SC2A8QBKa5yzUBddcCHsd0FJccq5-JtzWHo4q_dlC9XvyjnYMJ8Ocyd2UBxMb3Uw-5_iD5fNhzw</recordid><startdate>19900701</startdate><enddate>19900701</enddate><creator>Inns, R.H.</creator><creator>Tuckwell, N.J.</creator><creator>Bright, J.E.</creator><creator>Marrs, T.C.</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>19900701</creationdate><title>Histochemical Demonstration of Calcium Accumulation in Muscle Fibres after Experimental Organophosphate Poisoning</title><author>Inns, R.H. ; Tuckwell, N.J. ; Bright, J.E. ; Marrs, T.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c369t-f92711b9100cebbf381d803a6fe4b97478eb3c8857bf40daee3210d0dc0158713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Acetylcholinesterase - metabolism</topic><topic>Animals</topic><topic>Calcium - metabolism</topic><topic>Diaphragm - enzymology</topic><topic>Histocytochemistry</topic><topic>Lethal Dose 50</topic><topic>Male</topic><topic>Mice</topic><topic>Muscles - metabolism</topic><topic>Muscles - pathology</topic><topic>Organophosphate Poisoning</topic><topic>Sarin - poisoning</topic><topic>Sarin - toxicity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Inns, R.H.</creatorcontrib><creatorcontrib>Tuckwell, N.J.</creatorcontrib><creatorcontrib>Bright, J.E.</creatorcontrib><creatorcontrib>Marrs, T.C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><jtitle>Human & experimental toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Inns, R.H.</au><au>Tuckwell, N.J.</au><au>Bright, J.E.</au><au>Marrs, T.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Histochemical Demonstration of Calcium Accumulation in Muscle Fibres after Experimental Organophosphate Poisoning</atitle><jtitle>Human & experimental toxicology</jtitle><addtitle>Hum Exp Toxicol</addtitle><date>1990-07-01</date><risdate>1990</risdate><volume>9</volume><issue>4</issue><spage>245</spage><epage>250</epage><pages>245-250</pages><issn>0960-3271</issn><eissn>1477-0903</eissn><abstract>The LD50 of subcutaneously-injected sarin (GB: isopropyl methylphosphonofluoridate) in mice was 172 μg kg-1. Mice were treated with sarin at doses between 25 and 150 μg kg-1, administered subcutaneously. After sacrifice of the animals, the diaphragms were removed and stained for acetylcholinesterase activity and the presence of ionized calcium. Calcium was found in the diaphragms of those mice to which sarin had been administered at doses of 50 μg kg-1 or above. Calcium accumulation was not present in diaphragms from those animals that had received 25 μg kg-1. Calcium accumulation occurred earliest and remained longest in diaphragms from those animals receiving the highest doses. Accumulation of calcium was associated with end-plates, as demonstrated by an acetylcholinesterase histochemical method.</abstract><cop>Thousand Oaks, CA</cop><pub>SAGE Publications</pub><pmid>2390321</pmid><doi>10.1177/096032719000900407</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0960-3271 |
| ispartof | Human & experimental toxicology, 1990-07, Vol.9 (4), p.245-250 |
| issn | 0960-3271 1477-0903 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21097900 |
| source | Access via SAGE; MEDLINE; Alma/SFX Local Collection |
| subjects | Acetylcholinesterase - metabolism Animals Calcium - metabolism Diaphragm - enzymology Histocytochemistry Lethal Dose 50 Male Mice Muscles - metabolism Muscles - pathology Organophosphate Poisoning Sarin - poisoning Sarin - toxicity |
| title | Histochemical Demonstration of Calcium Accumulation in Muscle Fibres after Experimental Organophosphate Poisoning |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T11%3A52%3A06IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Histochemical%20Demonstration%20of%20Calcium%20Accumulation%20in%20Muscle%20Fibres%20after%20Experimental%20Organophosphate%20Poisoning&rft.jtitle=Human%20&%20experimental%20toxicology&rft.au=Inns,%20R.H.&rft.date=1990-07-01&rft.volume=9&rft.issue=4&rft.spage=245&rft.epage=250&rft.pages=245-250&rft.issn=0960-3271&rft.eissn=1477-0903&rft_id=info:doi/10.1177/096032719000900407&rft_dat=%3Cproquest_cross%3E21097900%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=21097900&rft_id=info:pmid/2390321&rft_sage_id=10.1177_096032719000900407&rfr_iscdi=true |