Low-Cost and Rapid-Production Microfluidic Electrochemical Double-Layer Capacitors for Fast and Sensitive Breast Cancer Diagnosis
This technical note describes a new microfluidic sensor that combines low-cost (USD $0.97) with rapid fabrication and user-friendly, fast, sensitive, and accurate quantification of a breast cancer biomarker. The electrodes consisted of cost-effective bare stainless-steel capillaries, whose mass prod...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2018-11, Vol.90 (21), p.12377-12384 |
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| creator | de Oliveira, Ricardo A. G Nicoliche, Caroline Y. N Pasqualeti, Anielli M Shimizu, Flavio M Ribeiro, Iris R Melendez, Matias E Carvalho, André L Gobbi, Angelo L Faria, Ronaldo C Lima, Renato S |
| description | This technical note describes a new microfluidic sensor that combines low-cost (USD $0.97) with rapid fabrication and user-friendly, fast, sensitive, and accurate quantification of a breast cancer biomarker. The electrodes consisted of cost-effective bare stainless-steel capillaries, whose mass production is already well-established. These capillaries were used as received, without any surface modification. Microfluidic chips containing electrical double-layer capillary capacitors (μEDLC) were obtained by a cleanroom-free prototyping that allows the fabrication of dozens to hundreds of chips in 1 h. This sensor provided the successful quantification of CA 15-3, a biomarker protein for breast cancer, in serum samples from cancer patients. Antibody-anchored magnetic beads were utilized for immunocapture of the marker, and then, water was added to dilute the protein. Next, the CA 15-3 detection ( |
| doi_str_mv | 10.1021/acs.analchem.8b02605 |
| format | Article |
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G ; Nicoliche, Caroline Y. N ; Pasqualeti, Anielli M ; Shimizu, Flavio M ; Ribeiro, Iris R ; Melendez, Matias E ; Carvalho, André L ; Gobbi, Angelo L ; Faria, Ronaldo C ; Lima, Renato S</creator><creatorcontrib>de Oliveira, Ricardo A. G ; Nicoliche, Caroline Y. N ; Pasqualeti, Anielli M ; Shimizu, Flavio M ; Ribeiro, Iris R ; Melendez, Matias E ; Carvalho, André L ; Gobbi, Angelo L ; Faria, Ronaldo C ; Lima, Renato S</creatorcontrib><description>This technical note describes a new microfluidic sensor that combines low-cost (USD $0.97) with rapid fabrication and user-friendly, fast, sensitive, and accurate quantification of a breast cancer biomarker. The electrodes consisted of cost-effective bare stainless-steel capillaries, whose mass production is already well-established. These capillaries were used as received, without any surface modification. Microfluidic chips containing electrical double-layer capillary capacitors (μEDLC) were obtained by a cleanroom-free prototyping that allows the fabrication of dozens to hundreds of chips in 1 h. This sensor provided the successful quantification of CA 15-3, a biomarker protein for breast cancer, in serum samples from cancer patients. Antibody-anchored magnetic beads were utilized for immunocapture of the marker, and then, water was added to dilute the protein. Next, the CA 15-3 detection (<2 min) was made without using redox probes, antibody on electrode (sandwich immunoassay), or signal amplification strategies. In addition, the capacitance tests eliminated external pumping systems and precise volumetric sampling steps, as well as presented low sample volume (5 μL) and high sensitivity using bare capillaries in a new design for double-layer capacitors. The achieved limit-of-detection (92.0 μU mL–1) is lower than that of most methods reported in the literature for CA 15-3, which are based on nanostructured electrodes. The data shown in this technical note support the potential of the μEDLC toward breast cancer diagnosis even at early stages. We believe that accurate analyses using a simple sample pretreatment such as magnetic field-assisted immunocapture and cost-effective bare electrodes can be extended to quantify other cancer biomarkers and even biomolecules by changing the biorecognition element.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/acs.analchem.8b02605</identifier><identifier>PMID: 30222327</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Analytical chemistry ; Beads ; Biomarkers ; Biomolecules ; Breast cancer ; Cancer ; Capacitance ; Capacitors ; Capillaries ; Cleanrooms ; Diagnosis ; Electrical equipment ; Electrochemistry ; Electrodes ; Fabrication ; Immunoassay ; Low cost ; Magnetic fields ; Mass production ; Medical diagnosis ; Microfluidics ; Pretreatment ; Proteins ; Prototyping ; Sensors ; Steel</subject><ispartof>Analytical chemistry (Washington), 2018-11, Vol.90 (21), p.12377-12384</ispartof><rights>Copyright American Chemical Society Nov 6, 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a442t-dc9dbb43710d5b898c3289a0addb0f21119702bd3f9488d296dd278b3fcbd0333</citedby><cites>FETCH-LOGICAL-a442t-dc9dbb43710d5b898c3289a0addb0f21119702bd3f9488d296dd278b3fcbd0333</cites><orcidid>0000-0001-8450-1475 ; 0000-0002-4833-9893 ; 0000-0002-0643-6185 ; 0000-0003-1094-9597 ; 0000-0001-7251-3727 ; 0000-0001-7214-6402</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/acs.analchem.8b02605$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/acs.analchem.8b02605$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>315,781,785,2766,27081,27929,27930,56743,56793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30222327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>de Oliveira, Ricardo A. G</creatorcontrib><creatorcontrib>Nicoliche, Caroline Y. N</creatorcontrib><creatorcontrib>Pasqualeti, Anielli M</creatorcontrib><creatorcontrib>Shimizu, Flavio M</creatorcontrib><creatorcontrib>Ribeiro, Iris R</creatorcontrib><creatorcontrib>Melendez, Matias E</creatorcontrib><creatorcontrib>Carvalho, André L</creatorcontrib><creatorcontrib>Gobbi, Angelo L</creatorcontrib><creatorcontrib>Faria, Ronaldo C</creatorcontrib><creatorcontrib>Lima, Renato S</creatorcontrib><title>Low-Cost and Rapid-Production Microfluidic Electrochemical Double-Layer Capacitors for Fast and Sensitive Breast Cancer Diagnosis</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>This technical note describes a new microfluidic sensor that combines low-cost (USD $0.97) with rapid fabrication and user-friendly, fast, sensitive, and accurate quantification of a breast cancer biomarker. 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In addition, the capacitance tests eliminated external pumping systems and precise volumetric sampling steps, as well as presented low sample volume (5 μL) and high sensitivity using bare capillaries in a new design for double-layer capacitors. The achieved limit-of-detection (92.0 μU mL–1) is lower than that of most methods reported in the literature for CA 15-3, which are based on nanostructured electrodes. The data shown in this technical note support the potential of the μEDLC toward breast cancer diagnosis even at early stages. We believe that accurate analyses using a simple sample pretreatment such as magnetic field-assisted immunocapture and cost-effective bare electrodes can be extended to quantify other cancer biomarkers and even biomolecules by changing the biorecognition element.</description><subject>Analytical chemistry</subject><subject>Beads</subject><subject>Biomarkers</subject><subject>Biomolecules</subject><subject>Breast cancer</subject><subject>Cancer</subject><subject>Capacitance</subject><subject>Capacitors</subject><subject>Capillaries</subject><subject>Cleanrooms</subject><subject>Diagnosis</subject><subject>Electrical equipment</subject><subject>Electrochemistry</subject><subject>Electrodes</subject><subject>Fabrication</subject><subject>Immunoassay</subject><subject>Low cost</subject><subject>Magnetic fields</subject><subject>Mass production</subject><subject>Medical diagnosis</subject><subject>Microfluidics</subject><subject>Pretreatment</subject><subject>Proteins</subject><subject>Prototyping</subject><subject>Sensors</subject><subject>Steel</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp9kU1P3DAQhq0KVBbaf4AqS1y4ZBmPsxvnWMKntFURbc_R-CPUKBtv7aSII_-8iXbh0AOnkUbP-440D2PHAuYCUJyRSXPqqDW_3XquNOASFh_YTCwQsqVSuMdmACAzLAAO2GFKjwBCgFh-ZAcSEFFiMWMvq_CUVSH1nDrL72njbXYXgx1M70PHv3kTQ9MO3nrDL1tn-himg95Qyy_CoFuXrejZRV7RhozvQ0y8CZFf0a7yh-uS7_1fx8-jm5YVdWbkLzw9dCH59IntN9Qm93k3j9ivq8uf1U22-n59W31dZZTn2GfWlFbrXBYC7EKrUhmJqiQgazU0KIQoC0BtZVPmSlksl9ZiobRsjLYgpTxip9veTQx_Bpf6eu2TcW1LnQtDqlFAKSWIIh_Rk__QxzDE8dcTJRHzhYBipPItNb4opeiaehP9muJzLaCeFNWjovpVUb1TNMa-7MoHvXb2LfTqZARgC0zxt8Pvdv4DyRmg7w</recordid><startdate>20181106</startdate><enddate>20181106</enddate><creator>de Oliveira, Ricardo A. 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G ; Nicoliche, Caroline Y. N ; Pasqualeti, Anielli M ; Shimizu, Flavio M ; Ribeiro, Iris R ; Melendez, Matias E ; Carvalho, André L ; Gobbi, Angelo L ; Faria, Ronaldo C ; Lima, Renato S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a442t-dc9dbb43710d5b898c3289a0addb0f21119702bd3f9488d296dd278b3fcbd0333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Analytical chemistry</topic><topic>Beads</topic><topic>Biomarkers</topic><topic>Biomolecules</topic><topic>Breast cancer</topic><topic>Cancer</topic><topic>Capacitance</topic><topic>Capacitors</topic><topic>Capillaries</topic><topic>Cleanrooms</topic><topic>Diagnosis</topic><topic>Electrical equipment</topic><topic>Electrochemistry</topic><topic>Electrodes</topic><topic>Fabrication</topic><topic>Immunoassay</topic><topic>Low cost</topic><topic>Magnetic fields</topic><topic>Mass production</topic><topic>Medical diagnosis</topic><topic>Microfluidics</topic><topic>Pretreatment</topic><topic>Proteins</topic><topic>Prototyping</topic><topic>Sensors</topic><topic>Steel</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>de Oliveira, Ricardo A. 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G</au><au>Nicoliche, Caroline Y. N</au><au>Pasqualeti, Anielli M</au><au>Shimizu, Flavio M</au><au>Ribeiro, Iris R</au><au>Melendez, Matias E</au><au>Carvalho, André L</au><au>Gobbi, Angelo L</au><au>Faria, Ronaldo C</au><au>Lima, Renato S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Low-Cost and Rapid-Production Microfluidic Electrochemical Double-Layer Capacitors for Fast and Sensitive Breast Cancer Diagnosis</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2018-11-06</date><risdate>2018</risdate><volume>90</volume><issue>21</issue><spage>12377</spage><epage>12384</epage><pages>12377-12384</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><abstract>This technical note describes a new microfluidic sensor that combines low-cost (USD $0.97) with rapid fabrication and user-friendly, fast, sensitive, and accurate quantification of a breast cancer biomarker. The electrodes consisted of cost-effective bare stainless-steel capillaries, whose mass production is already well-established. These capillaries were used as received, without any surface modification. Microfluidic chips containing electrical double-layer capillary capacitors (μEDLC) were obtained by a cleanroom-free prototyping that allows the fabrication of dozens to hundreds of chips in 1 h. This sensor provided the successful quantification of CA 15-3, a biomarker protein for breast cancer, in serum samples from cancer patients. Antibody-anchored magnetic beads were utilized for immunocapture of the marker, and then, water was added to dilute the protein. Next, the CA 15-3 detection (<2 min) was made without using redox probes, antibody on electrode (sandwich immunoassay), or signal amplification strategies. In addition, the capacitance tests eliminated external pumping systems and precise volumetric sampling steps, as well as presented low sample volume (5 μL) and high sensitivity using bare capillaries in a new design for double-layer capacitors. The achieved limit-of-detection (92.0 μU mL–1) is lower than that of most methods reported in the literature for CA 15-3, which are based on nanostructured electrodes. The data shown in this technical note support the potential of the μEDLC toward breast cancer diagnosis even at early stages. We believe that accurate analyses using a simple sample pretreatment such as magnetic field-assisted immunocapture and cost-effective bare electrodes can be extended to quantify other cancer biomarkers and even biomolecules by changing the biorecognition element.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>30222327</pmid><doi>10.1021/acs.analchem.8b02605</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-8450-1475</orcidid><orcidid>https://orcid.org/0000-0002-4833-9893</orcidid><orcidid>https://orcid.org/0000-0002-0643-6185</orcidid><orcidid>https://orcid.org/0000-0003-1094-9597</orcidid><orcidid>https://orcid.org/0000-0001-7251-3727</orcidid><orcidid>https://orcid.org/0000-0001-7214-6402</orcidid></addata></record> |
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| subjects | Analytical chemistry Beads Biomarkers Biomolecules Breast cancer Cancer Capacitance Capacitors Capillaries Cleanrooms Diagnosis Electrical equipment Electrochemistry Electrodes Fabrication Immunoassay Low cost Magnetic fields Mass production Medical diagnosis Microfluidics Pretreatment Proteins Prototyping Sensors Steel |
| title | Low-Cost and Rapid-Production Microfluidic Electrochemical Double-Layer Capacitors for Fast and Sensitive Breast Cancer Diagnosis |
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