Intrapatient comparison of Mycobacterium leprae by VNTR analysis in nasal secretions and skin biopsy in a Brazilian leprosy endemic region
This study compares the strains of genotypes of M. leprae from nasal secretions (NS) and skin biopsy (SB) in the same patient, supplementing conventional epidemiology to gain insight into the infection of leprosy in Fortaleza, Brazil. The sample consisted of 38 newly diagnosed leprosy patients atten...
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| Veröffentlicht in: | Leprosy review 2016-12, Vol.87 (4), p.486-500 |
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| creator | Lima, Luana N G C Fontes, Amanda N B Li, Wei Suffys, Philip N Vissa, Varalakshmi D Mota, Rosa M S Almeida, Rosa L F Pontes, Maria A Gonçales, Heitor De S Frota, Cristiane C Rodrigues, Laura C Kendall, Carl Kerr, Ligia R F S |
| description | This study compares the strains of genotypes of M. leprae from nasal secretions (NS) and skin biopsy (SB) in the same patient, supplementing conventional epidemiology to gain insight into the infection of leprosy in Fortaleza, Brazil.
The sample consisted of 38 newly diagnosed leprosy patients attending the National Reference Center of Dermatology Dona Libania (CDERM), in Fortaleza, who tested positive for M. leprae by PCR in DNA extracts of nasal secretions. DNA was also extracted from skin biopsy (SB) scrapings of each patient and used for multiplex PCR amplification of M. leprae VNTR loci. The number of repeats at 15 loci were determined by the fragment length analysis method.
Locus VNTR genotypes were achieved in 38 NS, and in 38 SB specimens.
M. leprae strains differed in their genotypes in paired specimens in all but two of
38 patients. The genotype similarity in the remainder ranged from 53% to 87%.
M. leprae 15 VNTR loci genotypes of paired nasal and biopsy skin samples from five patients were identical, while as many as seven loci differed in the 33 other patients. When the NS and biopsy genotypes were pooled and compared, it was found that there was a great variability among different VNTR markers. It is important to investigate other molecular markers suitable for typing genetic variations of the bacilli. |
| doi_str_mv | 10.47276/lr.87.4.486 |
| format | Article |
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The sample consisted of 38 newly diagnosed leprosy patients attending the National Reference Center of Dermatology Dona Libania (CDERM), in Fortaleza, who tested positive for M. leprae by PCR in DNA extracts of nasal secretions. DNA was also extracted from skin biopsy (SB) scrapings of each patient and used for multiplex PCR amplification of M. leprae VNTR loci. The number of repeats at 15 loci were determined by the fragment length analysis method.
Locus VNTR genotypes were achieved in 38 NS, and in 38 SB specimens.
M. leprae strains differed in their genotypes in paired specimens in all but two of
38 patients. The genotype similarity in the remainder ranged from 53% to 87%.
M. leprae 15 VNTR loci genotypes of paired nasal and biopsy skin samples from five patients were identical, while as many as seven loci differed in the 33 other patients. When the NS and biopsy genotypes were pooled and compared, it was found that there was a great variability among different VNTR markers. It is important to investigate other molecular markers suitable for typing genetic variations of the bacilli.</description><identifier>ISSN: 0305-7518</identifier><identifier>ISSN: 2162-8807</identifier><identifier>EISSN: 2162-8807</identifier><identifier>DOI: 10.47276/lr.87.4.486</identifier><identifier>PMID: 30226353</identifier><language>eng</language><publisher>England: British Leprosy Relief Association</publisher><subject>Analysis ; Biopsy - methods ; Brazil - epidemiology ; Comparative analysis ; Cross-Sectional Studies ; Diagnosis ; DNA, Bacterial - genetics ; Endemic Diseases ; Epidemiology ; Genetic aspects ; Genetic Variation ; Genotype ; Health aspects ; Humans ; Leprosy ; Leprosy - diagnosis ; Leprosy - microbiology ; Minisatellite Repeats ; Mycobacterium leprae ; Mycobacterium leprae - classification ; Mycobacterium leprae - genetics ; Mycobacterium leprae - isolation & purification ; Nose - microbiology ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Skin - microbiology ; Skin - pathology ; Skin biopsy</subject><ispartof>Leprosy review, 2016-12, Vol.87 (4), p.486-500</ispartof><rights>COPYRIGHT 2016 British Leprosy Relief Association</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-1e80a9fd83e819ad53e783626002c3a8dea31f891f1d23dd32c96328e711dfe13</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30226353$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lima, Luana N G C</creatorcontrib><creatorcontrib>Fontes, Amanda N B</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Suffys, Philip N</creatorcontrib><creatorcontrib>Vissa, Varalakshmi D</creatorcontrib><creatorcontrib>Mota, Rosa M S</creatorcontrib><creatorcontrib>Almeida, Rosa L F</creatorcontrib><creatorcontrib>Pontes, Maria A</creatorcontrib><creatorcontrib>Gonçales, Heitor De S</creatorcontrib><creatorcontrib>Frota, Cristiane C</creatorcontrib><creatorcontrib>Rodrigues, Laura C</creatorcontrib><creatorcontrib>Kendall, Carl</creatorcontrib><creatorcontrib>Kerr, Ligia R F S</creatorcontrib><title>Intrapatient comparison of Mycobacterium leprae by VNTR analysis in nasal secretions and skin biopsy in a Brazilian leprosy endemic region</title><title>Leprosy review</title><addtitle>Lepr Rev</addtitle><description>This study compares the strains of genotypes of M. leprae from nasal secretions (NS) and skin biopsy (SB) in the same patient, supplementing conventional epidemiology to gain insight into the infection of leprosy in Fortaleza, Brazil.
The sample consisted of 38 newly diagnosed leprosy patients attending the National Reference Center of Dermatology Dona Libania (CDERM), in Fortaleza, who tested positive for M. leprae by PCR in DNA extracts of nasal secretions. DNA was also extracted from skin biopsy (SB) scrapings of each patient and used for multiplex PCR amplification of M. leprae VNTR loci. The number of repeats at 15 loci were determined by the fragment length analysis method.
Locus VNTR genotypes were achieved in 38 NS, and in 38 SB specimens.
M. leprae strains differed in their genotypes in paired specimens in all but two of
38 patients. The genotype similarity in the remainder ranged from 53% to 87%.
M. leprae 15 VNTR loci genotypes of paired nasal and biopsy skin samples from five patients were identical, while as many as seven loci differed in the 33 other patients. When the NS and biopsy genotypes were pooled and compared, it was found that there was a great variability among different VNTR markers. It is important to investigate other molecular markers suitable for typing genetic variations of the bacilli.</description><subject>Analysis</subject><subject>Biopsy - methods</subject><subject>Brazil - epidemiology</subject><subject>Comparative analysis</subject><subject>Cross-Sectional Studies</subject><subject>Diagnosis</subject><subject>DNA, Bacterial - genetics</subject><subject>Endemic Diseases</subject><subject>Epidemiology</subject><subject>Genetic aspects</subject><subject>Genetic Variation</subject><subject>Genotype</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Leprosy</subject><subject>Leprosy - diagnosis</subject><subject>Leprosy - microbiology</subject><subject>Minisatellite Repeats</subject><subject>Mycobacterium leprae</subject><subject>Mycobacterium leprae - classification</subject><subject>Mycobacterium leprae - genetics</subject><subject>Mycobacterium leprae - isolation & purification</subject><subject>Nose - microbiology</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Single Nucleotide</subject><subject>Skin - microbiology</subject><subject>Skin - pathology</subject><subject>Skin biopsy</subject><issn>0305-7518</issn><issn>2162-8807</issn><issn>2162-8807</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkUtv1TAQhSMEoreFHWtkCQmxIMGPJHaWpeJRqYCECltrrjNpDY6d2ski_IT-anzvLYhKyAtLM98ZzZxTFM8YrWrJZfvGxUrJqq5q1T4oNpy1vFSKyofFhgralLJh6qg4TukHpYKxTjwujgTlvBWN2BS3536OMMFs0c_EhHGCaFPwJAzk02rCFsyM0S4jcThFQLJdyffPl18JeHBrsolYTzwkcCShiTjb4FNu9iT9zJ2tDVNadwyQtxF-WWfB70eFXEbf42gNiXiVZU-KRwO4hE_v_pPi2_t3l2cfy4svH87PTi9KU3M5lwwVhW7olUDFOugbgVKJlreUciNA9QiCDapjA-u56HvBTdcKrlAy1g_IxEnx6jA3L3GzYJr1aJNB58BjWJLmjHaCy441GX1xQK_AobZ-CNkss8P1aUNZ02Wfd1T1Hyq__XXB42Bz_Z7g5T-CawQ3X6fglr1598HXB9Bkv1LEQU_RjhBXzajep69d1ErqWuf0M_787rJlO2L_F_4Tt_gNVMGqdQ</recordid><startdate>201612</startdate><enddate>201612</enddate><creator>Lima, Luana N G C</creator><creator>Fontes, Amanda N B</creator><creator>Li, Wei</creator><creator>Suffys, Philip N</creator><creator>Vissa, Varalakshmi D</creator><creator>Mota, Rosa M S</creator><creator>Almeida, Rosa L F</creator><creator>Pontes, Maria A</creator><creator>Gonçales, Heitor De S</creator><creator>Frota, Cristiane C</creator><creator>Rodrigues, Laura C</creator><creator>Kendall, Carl</creator><creator>Kerr, Ligia R F S</creator><general>British Leprosy Relief Association</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201612</creationdate><title>Intrapatient comparison of Mycobacterium leprae by VNTR analysis in nasal secretions and skin biopsy in a Brazilian leprosy endemic region</title><author>Lima, Luana N G C ; Fontes, Amanda N B ; Li, Wei ; Suffys, Philip N ; Vissa, Varalakshmi D ; Mota, Rosa M S ; Almeida, Rosa L F ; Pontes, Maria A ; Gonçales, Heitor De S ; Frota, Cristiane C ; Rodrigues, Laura C ; Kendall, Carl ; Kerr, Ligia R F S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-1e80a9fd83e819ad53e783626002c3a8dea31f891f1d23dd32c96328e711dfe13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Analysis</topic><topic>Biopsy - methods</topic><topic>Brazil - epidemiology</topic><topic>Comparative analysis</topic><topic>Cross-Sectional Studies</topic><topic>Diagnosis</topic><topic>DNA, Bacterial - genetics</topic><topic>Endemic Diseases</topic><topic>Epidemiology</topic><topic>Genetic aspects</topic><topic>Genetic Variation</topic><topic>Genotype</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Leprosy</topic><topic>Leprosy - diagnosis</topic><topic>Leprosy - microbiology</topic><topic>Minisatellite Repeats</topic><topic>Mycobacterium leprae</topic><topic>Mycobacterium leprae - classification</topic><topic>Mycobacterium leprae - genetics</topic><topic>Mycobacterium leprae - isolation & purification</topic><topic>Nose - microbiology</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Single Nucleotide</topic><topic>Skin - microbiology</topic><topic>Skin - pathology</topic><topic>Skin biopsy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lima, Luana N G C</creatorcontrib><creatorcontrib>Fontes, Amanda N B</creatorcontrib><creatorcontrib>Li, Wei</creatorcontrib><creatorcontrib>Suffys, Philip N</creatorcontrib><creatorcontrib>Vissa, Varalakshmi D</creatorcontrib><creatorcontrib>Mota, Rosa M S</creatorcontrib><creatorcontrib>Almeida, Rosa L F</creatorcontrib><creatorcontrib>Pontes, Maria A</creatorcontrib><creatorcontrib>Gonçales, Heitor De S</creatorcontrib><creatorcontrib>Frota, Cristiane C</creatorcontrib><creatorcontrib>Rodrigues, Laura C</creatorcontrib><creatorcontrib>Kendall, Carl</creatorcontrib><creatorcontrib>Kerr, Ligia R F S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Leprosy review</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lima, Luana N G C</au><au>Fontes, Amanda N B</au><au>Li, Wei</au><au>Suffys, Philip N</au><au>Vissa, Varalakshmi D</au><au>Mota, Rosa M S</au><au>Almeida, Rosa L F</au><au>Pontes, Maria A</au><au>Gonçales, Heitor De S</au><au>Frota, Cristiane C</au><au>Rodrigues, Laura C</au><au>Kendall, Carl</au><au>Kerr, Ligia R F S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intrapatient comparison of Mycobacterium leprae by VNTR analysis in nasal secretions and skin biopsy in a Brazilian leprosy endemic region</atitle><jtitle>Leprosy review</jtitle><addtitle>Lepr Rev</addtitle><date>2016-12</date><risdate>2016</risdate><volume>87</volume><issue>4</issue><spage>486</spage><epage>500</epage><pages>486-500</pages><issn>0305-7518</issn><issn>2162-8807</issn><eissn>2162-8807</eissn><abstract>This study compares the strains of genotypes of M. leprae from nasal secretions (NS) and skin biopsy (SB) in the same patient, supplementing conventional epidemiology to gain insight into the infection of leprosy in Fortaleza, Brazil.
The sample consisted of 38 newly diagnosed leprosy patients attending the National Reference Center of Dermatology Dona Libania (CDERM), in Fortaleza, who tested positive for M. leprae by PCR in DNA extracts of nasal secretions. DNA was also extracted from skin biopsy (SB) scrapings of each patient and used for multiplex PCR amplification of M. leprae VNTR loci. The number of repeats at 15 loci were determined by the fragment length analysis method.
Locus VNTR genotypes were achieved in 38 NS, and in 38 SB specimens.
M. leprae strains differed in their genotypes in paired specimens in all but two of
38 patients. The genotype similarity in the remainder ranged from 53% to 87%.
M. leprae 15 VNTR loci genotypes of paired nasal and biopsy skin samples from five patients were identical, while as many as seven loci differed in the 33 other patients. When the NS and biopsy genotypes were pooled and compared, it was found that there was a great variability among different VNTR markers. It is important to investigate other molecular markers suitable for typing genetic variations of the bacilli.</abstract><cop>England</cop><pub>British Leprosy Relief Association</pub><pmid>30226353</pmid><doi>10.47276/lr.87.4.486</doi><tpages>15</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Analysis Biopsy - methods Brazil - epidemiology Comparative analysis Cross-Sectional Studies Diagnosis DNA, Bacterial - genetics Endemic Diseases Epidemiology Genetic aspects Genetic Variation Genotype Health aspects Humans Leprosy Leprosy - diagnosis Leprosy - microbiology Minisatellite Repeats Mycobacterium leprae Mycobacterium leprae - classification Mycobacterium leprae - genetics Mycobacterium leprae - isolation & purification Nose - microbiology Polymerase Chain Reaction Polymorphism, Single Nucleotide Skin - microbiology Skin - pathology Skin biopsy |
| title | Intrapatient comparison of Mycobacterium leprae by VNTR analysis in nasal secretions and skin biopsy in a Brazilian leprosy endemic region |
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