Maintenance of pluripotency and self-renewal ability of mouse embryonic stem cells in the absence of tetraspanin CD9
We have previously demonstrated that the tetraspanin CD9 is necessary for membrane fusion between sperm and oocyte during fertilization. While knockout mice for CD9 are viable, CD9 −/− females are sterile due to the inability of their oocytes to fuse with sperm. While CD9 is not essential for subseq...
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| Veröffentlicht in: | Differentiation (London) 2009-09, Vol.78 (2), p.137-142 |
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| creator | Akutsu, Hidenori Miura, Takumi Machida, Masakazu Birumachi, Jun-ichi Hamada, Aki Yamada, Mitsutoshi Sullivan, Stephen Miyado, Kenji Umezawa, Akihiro |
| description | We have previously demonstrated that the tetraspanin CD9 is necessary for membrane fusion between sperm and oocyte during fertilization. While knockout mice for
CD9 are viable,
CD9
−/− females are sterile due to the inability of their oocytes to fuse with sperm. While CD9 is not essential for subsequent development, a role in embryonic stem (ES) cell self-renewal was hypothesised on the basis of two observations: CD9 is highly expressed in murine and human ES cells and the CD9-blocking antibody inhibits mouse ES cell colony formation and survival. To investigate whether CD9 has a direct effect on ES cells, we generated and characterised several
CD9 knockout murine ES cell lines. These
CD9
−/− ES cell lines exhibited equivalent morphology and growth properties to wild-type ES cells. Furthermore, the
CD9
−/− ES cell lines also displayed similar expression of pluripotency factors Oct3/4, Sox2 and Nanog.
CD9
−/− ES cells were found to be pluripotent
in vivo, as their cells injected into immunocompromised mice gave rise to teratomas consisting of tissues representative of all three germ layers. Additionally several high contribution mouse chimeras were generated by blastocyst injection with several
CD9
−/− ES cell lines. Taken together, our results reveal that CD9 is dispensable for mouse ES cell self-renewal and pluripotency. The generation of
CD9
−/− ES cells should prove to be a useful tool with which to study the function of this protein and a range of other associated cellular processes. |
| doi_str_mv | 10.1016/j.diff.2009.08.005 |
| format | Article |
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CD9 are viable,
CD9
−/− females are sterile due to the inability of their oocytes to fuse with sperm. While CD9 is not essential for subsequent development, a role in embryonic stem (ES) cell self-renewal was hypothesised on the basis of two observations: CD9 is highly expressed in murine and human ES cells and the CD9-blocking antibody inhibits mouse ES cell colony formation and survival. To investigate whether CD9 has a direct effect on ES cells, we generated and characterised several
CD9 knockout murine ES cell lines. These
CD9
−/− ES cell lines exhibited equivalent morphology and growth properties to wild-type ES cells. Furthermore, the
CD9
−/− ES cell lines also displayed similar expression of pluripotency factors Oct3/4, Sox2 and Nanog.
CD9
−/− ES cells were found to be pluripotent
in vivo, as their cells injected into immunocompromised mice gave rise to teratomas consisting of tissues representative of all three germ layers. Additionally several high contribution mouse chimeras were generated by blastocyst injection with several
CD9
−/− ES cell lines. Taken together, our results reveal that CD9 is dispensable for mouse ES cell self-renewal and pluripotency. The generation of
CD9
−/− ES cells should prove to be a useful tool with which to study the function of this protein and a range of other associated cellular processes.</description><identifier>ISSN: 0301-4681</identifier><identifier>EISSN: 1432-0436</identifier><identifier>DOI: 10.1016/j.diff.2009.08.005</identifier><identifier>PMID: 19716222</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Alkaline Phosphatase - metabolism ; Animals ; Antigens, CD - physiology ; CD9 ; Cell Differentiation - physiology ; Cell Survival ; Embryonic stem cell ; Embryonic Stem Cells - cytology ; Female ; Gene Expression Profiling ; Germ Layers - cytology ; Green Fluorescent Proteins - metabolism ; Immunoenzyme Techniques ; In Situ Hybridization, Fluorescence ; Knockout ; Membrane Glycoproteins - antagonists & inhibitors ; Membrane Glycoproteins - physiology ; Mice ; Mice, Inbred ICR ; Mice, Knockout ; Mice, Nude ; Oligonucleotide Array Sequence Analysis ; Pluripotency ; Regeneration ; Self-renewal ; Teratoma - metabolism ; Teratoma - pathology ; Tetraspanin-29</subject><ispartof>Differentiation (London), 2009-09, Vol.78 (2), p.137-142</ispartof><rights>2009 International Society of Differentiation</rights><rights>2009 International Society of Differentiation. Published by Elsevier Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c452t-6c51b11b9b0b73b7f507661aa46d01bb65093053b86f6a0261a41f2e05d22f543</citedby><cites>FETCH-LOGICAL-c452t-6c51b11b9b0b73b7f507661aa46d01bb65093053b86f6a0261a41f2e05d22f543</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.diff.2009.08.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19716222$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Akutsu, Hidenori</creatorcontrib><creatorcontrib>Miura, Takumi</creatorcontrib><creatorcontrib>Machida, Masakazu</creatorcontrib><creatorcontrib>Birumachi, Jun-ichi</creatorcontrib><creatorcontrib>Hamada, Aki</creatorcontrib><creatorcontrib>Yamada, Mitsutoshi</creatorcontrib><creatorcontrib>Sullivan, Stephen</creatorcontrib><creatorcontrib>Miyado, Kenji</creatorcontrib><creatorcontrib>Umezawa, Akihiro</creatorcontrib><title>Maintenance of pluripotency and self-renewal ability of mouse embryonic stem cells in the absence of tetraspanin CD9</title><title>Differentiation (London)</title><addtitle>Differentiation</addtitle><description>We have previously demonstrated that the tetraspanin CD9 is necessary for membrane fusion between sperm and oocyte during fertilization. While knockout mice for
CD9 are viable,
CD9
−/− females are sterile due to the inability of their oocytes to fuse with sperm. While CD9 is not essential for subsequent development, a role in embryonic stem (ES) cell self-renewal was hypothesised on the basis of two observations: CD9 is highly expressed in murine and human ES cells and the CD9-blocking antibody inhibits mouse ES cell colony formation and survival. To investigate whether CD9 has a direct effect on ES cells, we generated and characterised several
CD9 knockout murine ES cell lines. These
CD9
−/− ES cell lines exhibited equivalent morphology and growth properties to wild-type ES cells. Furthermore, the
CD9
−/− ES cell lines also displayed similar expression of pluripotency factors Oct3/4, Sox2 and Nanog.
CD9
−/− ES cells were found to be pluripotent
in vivo, as their cells injected into immunocompromised mice gave rise to teratomas consisting of tissues representative of all three germ layers. Additionally several high contribution mouse chimeras were generated by blastocyst injection with several
CD9
−/− ES cell lines. Taken together, our results reveal that CD9 is dispensable for mouse ES cell self-renewal and pluripotency. The generation of
CD9
−/− ES cells should prove to be a useful tool with which to study the function of this protein and a range of other associated cellular processes.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Animals</subject><subject>Antigens, CD - physiology</subject><subject>CD9</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Survival</subject><subject>Embryonic stem cell</subject><subject>Embryonic Stem Cells - cytology</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Germ Layers - cytology</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Immunoenzyme Techniques</subject><subject>In Situ Hybridization, Fluorescence</subject><subject>Knockout</subject><subject>Membrane Glycoproteins - antagonists & inhibitors</subject><subject>Membrane Glycoproteins - physiology</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Mice, Knockout</subject><subject>Mice, Nude</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Pluripotency</subject><subject>Regeneration</subject><subject>Self-renewal</subject><subject>Teratoma - metabolism</subject><subject>Teratoma - pathology</subject><subject>Tetraspanin-29</subject><issn>0301-4681</issn><issn>1432-0436</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE2P1DAMhiPEih0W_gAHlBO3FjttM63EBQ2f0q647J6jJHVERm1akgxo_j2pZiRunCzZj1_ZD2NvEGoElO-P9eidqwXAUENfA3TP2A7bRlTQNvI520EDWLWyx1v2MqUjAPRS4At2i8MepRBix_KD9iFT0MESXxxfp1P061I69sx1GHmiyVWRAv3RE9fGTz6fN3BeTok4zSael-AtT5lmbmmaEveB559U4ETX1Ew56rTqUEaHT8MrduP0lOj1td6xpy-fHw_fqvsfX78fPt5Xtu1ErqTt0CCawYDZN2bvOthLiVq3cgQ0RnYwNNA1ppdOahBl1KITBN0ohOva5o69u-Sucfl1opTV7NN2ow5UzlcCYYC2FwUUF9DGJaVITq3RzzqeFYLaXKuj2lyrzbWCXhXXZentNf1kZhr_rVzlFuDDBaDy429PUSXrNyWjj2SzGhf_v_y_E42QQQ</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Akutsu, Hidenori</creator><creator>Miura, Takumi</creator><creator>Machida, Masakazu</creator><creator>Birumachi, Jun-ichi</creator><creator>Hamada, Aki</creator><creator>Yamada, Mitsutoshi</creator><creator>Sullivan, Stephen</creator><creator>Miyado, Kenji</creator><creator>Umezawa, Akihiro</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope></search><sort><creationdate>20090901</creationdate><title>Maintenance of pluripotency and self-renewal ability of mouse embryonic stem cells in the absence of tetraspanin CD9</title><author>Akutsu, Hidenori ; Miura, Takumi ; Machida, Masakazu ; Birumachi, Jun-ichi ; Hamada, Aki ; Yamada, Mitsutoshi ; Sullivan, Stephen ; Miyado, Kenji ; Umezawa, Akihiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c452t-6c51b11b9b0b73b7f507661aa46d01bb65093053b86f6a0261a41f2e05d22f543</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Alkaline Phosphatase - metabolism</topic><topic>Animals</topic><topic>Antigens, CD - physiology</topic><topic>CD9</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Survival</topic><topic>Embryonic stem cell</topic><topic>Embryonic Stem Cells - cytology</topic><topic>Female</topic><topic>Gene Expression Profiling</topic><topic>Germ Layers - cytology</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Immunoenzyme Techniques</topic><topic>In Situ Hybridization, Fluorescence</topic><topic>Knockout</topic><topic>Membrane Glycoproteins - antagonists & inhibitors</topic><topic>Membrane Glycoproteins - physiology</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Mice, Knockout</topic><topic>Mice, Nude</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Pluripotency</topic><topic>Regeneration</topic><topic>Self-renewal</topic><topic>Teratoma - metabolism</topic><topic>Teratoma - pathology</topic><topic>Tetraspanin-29</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Akutsu, Hidenori</creatorcontrib><creatorcontrib>Miura, Takumi</creatorcontrib><creatorcontrib>Machida, Masakazu</creatorcontrib><creatorcontrib>Birumachi, Jun-ichi</creatorcontrib><creatorcontrib>Hamada, Aki</creatorcontrib><creatorcontrib>Yamada, Mitsutoshi</creatorcontrib><creatorcontrib>Sullivan, Stephen</creatorcontrib><creatorcontrib>Miyado, Kenji</creatorcontrib><creatorcontrib>Umezawa, Akihiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><jtitle>Differentiation (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Akutsu, Hidenori</au><au>Miura, Takumi</au><au>Machida, Masakazu</au><au>Birumachi, Jun-ichi</au><au>Hamada, Aki</au><au>Yamada, Mitsutoshi</au><au>Sullivan, Stephen</au><au>Miyado, Kenji</au><au>Umezawa, Akihiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Maintenance of pluripotency and self-renewal ability of mouse embryonic stem cells in the absence of tetraspanin CD9</atitle><jtitle>Differentiation (London)</jtitle><addtitle>Differentiation</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>78</volume><issue>2</issue><spage>137</spage><epage>142</epage><pages>137-142</pages><issn>0301-4681</issn><eissn>1432-0436</eissn><abstract>We have previously demonstrated that the tetraspanin CD9 is necessary for membrane fusion between sperm and oocyte during fertilization. While knockout mice for
CD9 are viable,
CD9
−/− females are sterile due to the inability of their oocytes to fuse with sperm. While CD9 is not essential for subsequent development, a role in embryonic stem (ES) cell self-renewal was hypothesised on the basis of two observations: CD9 is highly expressed in murine and human ES cells and the CD9-blocking antibody inhibits mouse ES cell colony formation and survival. To investigate whether CD9 has a direct effect on ES cells, we generated and characterised several
CD9 knockout murine ES cell lines. These
CD9
−/− ES cell lines exhibited equivalent morphology and growth properties to wild-type ES cells. Furthermore, the
CD9
−/− ES cell lines also displayed similar expression of pluripotency factors Oct3/4, Sox2 and Nanog.
CD9
−/− ES cells were found to be pluripotent
in vivo, as their cells injected into immunocompromised mice gave rise to teratomas consisting of tissues representative of all three germ layers. Additionally several high contribution mouse chimeras were generated by blastocyst injection with several
CD9
−/− ES cell lines. Taken together, our results reveal that CD9 is dispensable for mouse ES cell self-renewal and pluripotency. The generation of
CD9
−/− ES cells should prove to be a useful tool with which to study the function of this protein and a range of other associated cellular processes.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>19716222</pmid><doi>10.1016/j.diff.2009.08.005</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0301-4681 |
| ispartof | Differentiation (London), 2009-09, Vol.78 (2), p.137-142 |
| issn | 0301-4681 1432-0436 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21090482 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Alkaline Phosphatase - metabolism Animals Antigens, CD - physiology CD9 Cell Differentiation - physiology Cell Survival Embryonic stem cell Embryonic Stem Cells - cytology Female Gene Expression Profiling Germ Layers - cytology Green Fluorescent Proteins - metabolism Immunoenzyme Techniques In Situ Hybridization, Fluorescence Knockout Membrane Glycoproteins - antagonists & inhibitors Membrane Glycoproteins - physiology Mice Mice, Inbred ICR Mice, Knockout Mice, Nude Oligonucleotide Array Sequence Analysis Pluripotency Regeneration Self-renewal Teratoma - metabolism Teratoma - pathology Tetraspanin-29 |
| title | Maintenance of pluripotency and self-renewal ability of mouse embryonic stem cells in the absence of tetraspanin CD9 |
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