Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis
This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied. This...
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| Veröffentlicht in: | Iranian biomedical journal 2019-03, Vol.23 (2), p.129-141 |
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| creator | Alexandr Alexandrovich, Basov Аnna Anatolyevna, Elkina Andrei Alexandrovich, Samkov Nikita Nikolaevich, Volchenko Arcadii Victorovich, Moiseev Liliya Viacheslavovna, Fedulova Mikhail Gennadievich, Baryshev Stepan Sergeevich, Dzhimak |
| description | This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied.
This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit.
Modification of isotope (deuterium-protium [D/H]) composition of rats’ blood and organ tissues with DDW (-705‰), introduced into rats’ diet within four weeks, leads to the formation of isotope D/H gradient between blood plasma and organ tissues and affects isotope D/H exchange reactions on the adaptive processes. The isolated liver mitochondria from the random bred rats consumed DDW presented a maximum increase in H2O2 production during the incubation in DDW medium. This increased level of H2O2 production was higher than that of the isolated liver mitochondria of the rats consuming natural deuterium content drinking water (-24‰).
The obtained results indicate the possibility of nutritional correction of isotope D/H metabolism in the blood by means of products with modified isotope composition, as well as the prospects of using isotope exchange reactions in case of imbalance in function of the body's defense systems in different generations of animals. |
| doi_str_mv | 10.29252/ibj.23.2.129 |
| format | Article |
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This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit.
Modification of isotope (deuterium-protium [D/H]) composition of rats’ blood and organ tissues with DDW (-705‰), introduced into rats’ diet within four weeks, leads to the formation of isotope D/H gradient between blood plasma and organ tissues and affects isotope D/H exchange reactions on the adaptive processes. The isolated liver mitochondria from the random bred rats consumed DDW presented a maximum increase in H2O2 production during the incubation in DDW medium. This increased level of H2O2 production was higher than that of the isolated liver mitochondria of the rats consuming natural deuterium content drinking water (-24‰).
The obtained results indicate the possibility of nutritional correction of isotope D/H metabolism in the blood by means of products with modified isotope composition, as well as the prospects of using isotope exchange reactions in case of imbalance in function of the body's defense systems in different generations of animals.</description><identifier>ISSN: 1028-852X</identifier><identifier>EISSN: 2008-823X</identifier><identifier>DOI: 10.29252/ibj.23.2.129</identifier><identifier>PMID: 30220191</identifier><language>eng</language><publisher>Iran: Pasteur Institute of Iran</publisher><subject>Animals ; Blood ; Blood plasma ; Depletion ; Deuterium ; Diet ; Drinking water ; Energy metabolism ; Exchanging ; Hydrogen peroxide ; Hydrogen production ; Isotope composition ; Laboratory animals ; Liver ; Metabolism ; Mitochondria ; Peroxidase ; Rodents ; Tissues</subject><ispartof>Iranian biomedical journal, 2019-03, Vol.23 (2), p.129-141</ispartof><rights>2019. This work is published under https://creativecommons.org/licenses/by-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2059-f2d4ef7405bb3302ad4d262b939a57722eb324da429d302cb2886b23e1e417513</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30220191$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Alexandr Alexandrovich, Basov</creatorcontrib><creatorcontrib>Аnna Anatolyevna, Elkina</creatorcontrib><creatorcontrib>Andrei Alexandrovich, Samkov</creatorcontrib><creatorcontrib>Nikita Nikolaevich, Volchenko</creatorcontrib><creatorcontrib>Arcadii Victorovich, Moiseev</creatorcontrib><creatorcontrib>Liliya Viacheslavovna, Fedulova</creatorcontrib><creatorcontrib>Mikhail Gennadievich, Baryshev</creatorcontrib><creatorcontrib>Stepan Sergeevich, Dzhimak</creatorcontrib><creatorcontrib>Kuban State Agrarian University, Krasnodar, Russian Federation, Russia; 4The V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow, Russian Federation, Russia</creatorcontrib><creatorcontrib>Kuban State University, Krasnodar, Russian Federation, Russia</creatorcontrib><title>Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis</title><title>Iranian biomedical journal</title><addtitle>Iran Biomed J</addtitle><description>This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied.
This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit.
Modification of isotope (deuterium-protium [D/H]) composition of rats’ blood and organ tissues with DDW (-705‰), introduced into rats’ diet within four weeks, leads to the formation of isotope D/H gradient between blood plasma and organ tissues and affects isotope D/H exchange reactions on the adaptive processes. The isolated liver mitochondria from the random bred rats consumed DDW presented a maximum increase in H2O2 production during the incubation in DDW medium. This increased level of H2O2 production was higher than that of the isolated liver mitochondria of the rats consuming natural deuterium content drinking water (-24‰).
The obtained results indicate the possibility of nutritional correction of isotope D/H metabolism in the blood by means of products with modified isotope composition, as well as the prospects of using isotope exchange reactions in case of imbalance in function of the body's defense systems in different generations of animals.</description><subject>Animals</subject><subject>Blood</subject><subject>Blood plasma</subject><subject>Depletion</subject><subject>Deuterium</subject><subject>Diet</subject><subject>Drinking water</subject><subject>Energy metabolism</subject><subject>Exchanging</subject><subject>Hydrogen peroxide</subject><subject>Hydrogen production</subject><subject>Isotope composition</subject><subject>Laboratory animals</subject><subject>Liver</subject><subject>Metabolism</subject><subject>Mitochondria</subject><subject>Peroxidase</subject><subject>Rodents</subject><subject>Tissues</subject><issn>1028-852X</issn><issn>2008-823X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>BENPR</sourceid><recordid>eNpdkUFv3CAQhVGUKtmmPfZaIeWSizcwmLV9jHbTZqWtUlWpmhvCZpywssEBHKl_pr-1bJP20BNo3jcPZh4hHzhbQgMSLm27X4JYwpJDc0QWwFhd1CDuj8mCM8h3Cfen5G2Me8aE5FV1Qk4FA2C84Qvya-v6YUbXIfU93eCcMNh5LDY4DZjQ0B86V6h3ND0i3Uaf_IR0c3lD136cfLTJZi237uxz5u5sjDNS7Qz94sP06Af_YDs9ZOdnHPw0oksH-ptOkepEN7bvMRyKX_O73sSDeOuSf0CH0cZ35E2vh4jvX88z8v3T9d36ptjdft6ur3ZFB0w2RQ-mxL4qmWxbkYfTpjSwgrYRjZZVBYCtgNLoEhqT5a6Ful61IJBjySvJxRm5ePGdgn-aMSY12tjhMGiHfo4KOKth1XAuMnr-H7r3c3D5dwryfqUUJchMFS9UF3yMAXs1BTvq8FNxpv4Ep3JwuUOBysFl_uOr69yOaP7Rf5MSvwFsFZRf</recordid><startdate>201903</startdate><enddate>201903</enddate><creator>Alexandr Alexandrovich, Basov</creator><creator>Аnna Anatolyevna, Elkina</creator><creator>Andrei Alexandrovich, Samkov</creator><creator>Nikita Nikolaevich, Volchenko</creator><creator>Arcadii Victorovich, Moiseev</creator><creator>Liliya Viacheslavovna, Fedulova</creator><creator>Mikhail Gennadievich, Baryshev</creator><creator>Stepan Sergeevich, Dzhimak</creator><general>Pasteur Institute of Iran</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7T5</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CWDGH</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201903</creationdate><title>Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis</title><author>Alexandr Alexandrovich, Basov ; Аnna Anatolyevna, Elkina ; Andrei Alexandrovich, Samkov ; Nikita Nikolaevich, Volchenko ; Arcadii Victorovich, Moiseev ; Liliya Viacheslavovna, Fedulova ; Mikhail Gennadievich, Baryshev ; Stepan Sergeevich, Dzhimak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2059-f2d4ef7405bb3302ad4d262b939a57722eb324da429d302cb2886b23e1e417513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Blood</topic><topic>Blood plasma</topic><topic>Depletion</topic><topic>Deuterium</topic><topic>Diet</topic><topic>Drinking water</topic><topic>Energy metabolism</topic><topic>Exchanging</topic><topic>Hydrogen peroxide</topic><topic>Hydrogen production</topic><topic>Isotope composition</topic><topic>Laboratory animals</topic><topic>Liver</topic><topic>Metabolism</topic><topic>Mitochondria</topic><topic>Peroxidase</topic><topic>Rodents</topic><topic>Tissues</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alexandr Alexandrovich, Basov</creatorcontrib><creatorcontrib>Аnna Anatolyevna, Elkina</creatorcontrib><creatorcontrib>Andrei Alexandrovich, Samkov</creatorcontrib><creatorcontrib>Nikita Nikolaevich, Volchenko</creatorcontrib><creatorcontrib>Arcadii Victorovich, Moiseev</creatorcontrib><creatorcontrib>Liliya Viacheslavovna, Fedulova</creatorcontrib><creatorcontrib>Mikhail Gennadievich, Baryshev</creatorcontrib><creatorcontrib>Stepan Sergeevich, Dzhimak</creatorcontrib><creatorcontrib>Kuban State Agrarian University, Krasnodar, Russian Federation, Russia; 4The V.M. 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Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow, Russian Federation, Russia</aucorp><aucorp>Kuban State University, Krasnodar, Russian Federation, Russia</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis</atitle><jtitle>Iranian biomedical journal</jtitle><addtitle>Iran Biomed J</addtitle><date>2019-03</date><risdate>2019</risdate><volume>23</volume><issue>2</issue><spage>129</spage><epage>141</epage><pages>129-141</pages><issn>1028-852X</issn><eissn>2008-823X</eissn><abstract>This study aimed to evaluate the reaction of organism of laboratory animals on deuterium-depleted drinking diet. To assess the cell energy metabolism, the effect of a liquid medium with different deuterium contents on isolated liver mitochondria of random bred rats and Wistar rats was studied.
This experimental study on the effect of deuterium-depleted drinking water (DDW) on 16-week-old male Wistar rats lasted for four weeks. Energy metabolism of mitochondria was examined through the production of hydrogen peroxide using an Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit.
Modification of isotope (deuterium-protium [D/H]) composition of rats’ blood and organ tissues with DDW (-705‰), introduced into rats’ diet within four weeks, leads to the formation of isotope D/H gradient between blood plasma and organ tissues and affects isotope D/H exchange reactions on the adaptive processes. The isolated liver mitochondria from the random bred rats consumed DDW presented a maximum increase in H2O2 production during the incubation in DDW medium. This increased level of H2O2 production was higher than that of the isolated liver mitochondria of the rats consuming natural deuterium content drinking water (-24‰).
The obtained results indicate the possibility of nutritional correction of isotope D/H metabolism in the blood by means of products with modified isotope composition, as well as the prospects of using isotope exchange reactions in case of imbalance in function of the body's defense systems in different generations of animals.</abstract><cop>Iran</cop><pub>Pasteur Institute of Iran</pub><pmid>30220191</pmid><doi>10.29252/ibj.23.2.129</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Animals Blood Blood plasma Depletion Deuterium Diet Drinking water Energy metabolism Exchanging Hydrogen peroxide Hydrogen production Isotope composition Laboratory animals Liver Metabolism Mitochondria Peroxidase Rodents Tissues |
| title | Influence of Deuterium-Depleted Water on the Isotope D/H Composition of Liver Tissue and Morphological Development of Rats at Different Periods of Ontogenesis |
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