Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems
Microalgae have been a popular edible food, but there are no known reports on the antioxidative peptides derived from microalgae. The algae protein waste, which is normally discarded as animal feed, is a by-product during production of algae essence from microalgae, Chlorella vulgaris. Algae protein...
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| Veröffentlicht in: | Bioresource technology 2009-07, Vol.100 (13), p.3419-3425 |
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| creator | Sheih, I.-Chuan Wu, Tung-Kung Fang, Tony J. |
| description | Microalgae have been a popular edible food, but there are no known reports on the antioxidative peptides derived from microalgae. The algae protein waste, which is normally discarded as animal feed, is a by-product during production of algae essence from microalgae,
Chlorella vulgaris. Algae protein waste was hydrolyzed using pepsin, and a potent antioxidative peptide of VECYGPNRPQF was separated and isolated. The peptide could efficiently quench a variety of free radicals, including hydroxyl radical, superoxide radical, peroxyl radical, DPPH radical and ABTS radicals, and performed more efficiently than that observed for BHT, Trolox and peptides from marine protein sources in most cases. The purified peptide also has significant protective effects on DNA and prevents cellular damage caused by hydroxyl radicals. In addition, the peptide has gastrointestinal enzyme-resistance and no cytotoxicity observed in human lung fibroblasts cell lines (WI-38)
in vitro. These results demonstrate that inexpensive algae protein waste could be a new alternative to produce antioxidative peptides. |
| doi_str_mv | 10.1016/j.biortech.2009.02.014 |
| format | Article |
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Chlorella vulgaris. Algae protein waste was hydrolyzed using pepsin, and a potent antioxidative peptide of VECYGPNRPQF was separated and isolated. The peptide could efficiently quench a variety of free radicals, including hydroxyl radical, superoxide radical, peroxyl radical, DPPH radical and ABTS radicals, and performed more efficiently than that observed for BHT, Trolox and peptides from marine protein sources in most cases. The purified peptide also has significant protective effects on DNA and prevents cellular damage caused by hydroxyl radicals. In addition, the peptide has gastrointestinal enzyme-resistance and no cytotoxicity observed in human lung fibroblasts cell lines (WI-38)
in vitro. These results demonstrate that inexpensive algae protein waste could be a new alternative to produce antioxidative peptides.</description><identifier>ISSN: 0960-8524</identifier><identifier>EISSN: 1873-2976</identifier><identifier>DOI: 10.1016/j.biortech.2009.02.014</identifier><identifier>PMID: 19299123</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Algae ; antioxidant activity ; Antioxidants - analysis ; Antioxidants - metabolism ; Antioxidative ; Applied sciences ; Biological and medical sciences ; Biotechnology - methods ; Biphenyl Compounds - chemistry ; Cell ; Cell Line, Tumor ; Chlorella vulgaris ; Chlorella vulgaris - metabolism ; Chromans - chemistry ; digestive enzymes ; DNA Damage ; Eukaryota - metabolism ; Exact sciences and technology ; Fibroblasts - drug effects ; Fundamental and applied biological sciences. Psychology ; gastrointestinal system ; Humans ; Hydroxyl Radical - chemistry ; Hydroxyl Radical - metabolism ; Inhibitory Concentration 50 ; microalgae ; oxidation ; Oxygen - chemistry ; pepsin ; Peptides ; Peptides - chemistry ; Picrates - chemistry ; Plant Extracts - pharmacology ; plant proteins ; Pollution ; proteinases ; Wastes</subject><ispartof>Bioresource technology, 2009-07, Vol.100 (13), p.3419-3425</ispartof><rights>2009 Elsevier Ltd</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-782d807a6a98012cd58b46c4b2861778bd138207b19dddaea0e5abea041c849a3</citedby><cites>FETCH-LOGICAL-c451t-782d807a6a98012cd58b46c4b2861778bd138207b19dddaea0e5abea041c849a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0960852409001151$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21510314$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19299123$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sheih, I.-Chuan</creatorcontrib><creatorcontrib>Wu, Tung-Kung</creatorcontrib><creatorcontrib>Fang, Tony J.</creatorcontrib><title>Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems</title><title>Bioresource technology</title><addtitle>Bioresour Technol</addtitle><description>Microalgae have been a popular edible food, but there are no known reports on the antioxidative peptides derived from microalgae. The algae protein waste, which is normally discarded as animal feed, is a by-product during production of algae essence from microalgae,
Chlorella vulgaris. Algae protein waste was hydrolyzed using pepsin, and a potent antioxidative peptide of VECYGPNRPQF was separated and isolated. The peptide could efficiently quench a variety of free radicals, including hydroxyl radical, superoxide radical, peroxyl radical, DPPH radical and ABTS radicals, and performed more efficiently than that observed for BHT, Trolox and peptides from marine protein sources in most cases. The purified peptide also has significant protective effects on DNA and prevents cellular damage caused by hydroxyl radicals. In addition, the peptide has gastrointestinal enzyme-resistance and no cytotoxicity observed in human lung fibroblasts cell lines (WI-38)
in vitro. These results demonstrate that inexpensive algae protein waste could be a new alternative to produce antioxidative peptides.</description><subject>Algae</subject><subject>antioxidant activity</subject><subject>Antioxidants - analysis</subject><subject>Antioxidants - metabolism</subject><subject>Antioxidative</subject><subject>Applied sciences</subject><subject>Biological and medical sciences</subject><subject>Biotechnology - methods</subject><subject>Biphenyl Compounds - chemistry</subject><subject>Cell</subject><subject>Cell Line, Tumor</subject><subject>Chlorella vulgaris</subject><subject>Chlorella vulgaris - metabolism</subject><subject>Chromans - chemistry</subject><subject>digestive enzymes</subject><subject>DNA Damage</subject><subject>Eukaryota - metabolism</subject><subject>Exact sciences and technology</subject><subject>Fibroblasts - drug effects</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gastrointestinal system</subject><subject>Humans</subject><subject>Hydroxyl Radical - chemistry</subject><subject>Hydroxyl Radical - metabolism</subject><subject>Inhibitory Concentration 50</subject><subject>microalgae</subject><subject>oxidation</subject><subject>Oxygen - chemistry</subject><subject>pepsin</subject><subject>Peptides</subject><subject>Peptides - chemistry</subject><subject>Picrates - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>plant proteins</subject><subject>Pollution</subject><subject>proteinases</subject><subject>Wastes</subject><issn>0960-8524</issn><issn>1873-2976</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhiMEokvhLxRf4Jbgcb6cG1XFl1SJA_RsTexJ61USB9vbNv8eL5vCkdNY9jPvjB5n2QXwAjg0H_ZFb52PpO8KwXlXcFFwqJ5lO5BtmYuubZ5nO941PJe1qM6yVyHsOecltOJldgad6DoQ5S5bL-do3aM1OEe2eLeQj5YCcwNDNtMDw6f3aO-JLbREa4gN3k0Mx1ukY1MkO7MHDJHY3Wq8G9eA6ZwujR0G8pSytww3s7AmcAqvsxcDjoHebPU8u_n86efV1_z6-5dvV5fXua5qiHkrhZG8xQY7yUFoU8u-anTVC9lA28reQCkFb3vojDFIyKnGPpUKtKw6LM-z96fctOivA4WoJhs0jSPO5A5BCeANNIInsDmB2rsQPA1q8XZCvyrg6ihd7dWTdHWUrrhQSXpqvNgmHPqJzL-2zXIC3m0ABo3j4HHWNvzlBNSQfuYY9PbEDegU3vrE3PwQHMo0HFr4Q3w8EZSM3VvyKmhLsyZjPemojLP_2_Y3zQGvmA</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>Sheih, I.-Chuan</creator><creator>Wu, Tung-Kung</creator><creator>Fang, Tony J.</creator><general>Elsevier Ltd</general><general>[New York, NY]: Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QH</scope><scope>7QO</scope><scope>7TV</scope><scope>7UA</scope><scope>8FD</scope><scope>C1K</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>M7N</scope><scope>P64</scope></search><sort><creationdate>20090701</creationdate><title>Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems</title><author>Sheih, I.-Chuan ; Wu, Tung-Kung ; Fang, Tony J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-782d807a6a98012cd58b46c4b2861778bd138207b19dddaea0e5abea041c849a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Algae</topic><topic>antioxidant activity</topic><topic>Antioxidants - analysis</topic><topic>Antioxidants - metabolism</topic><topic>Antioxidative</topic><topic>Applied sciences</topic><topic>Biological and medical sciences</topic><topic>Biotechnology - methods</topic><topic>Biphenyl Compounds - chemistry</topic><topic>Cell</topic><topic>Cell Line, Tumor</topic><topic>Chlorella vulgaris</topic><topic>Chlorella vulgaris - metabolism</topic><topic>Chromans - chemistry</topic><topic>digestive enzymes</topic><topic>DNA Damage</topic><topic>Eukaryota - metabolism</topic><topic>Exact sciences and technology</topic><topic>Fibroblasts - drug effects</topic><topic>Fundamental and applied biological sciences. 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The algae protein waste, which is normally discarded as animal feed, is a by-product during production of algae essence from microalgae,
Chlorella vulgaris. Algae protein waste was hydrolyzed using pepsin, and a potent antioxidative peptide of VECYGPNRPQF was separated and isolated. The peptide could efficiently quench a variety of free radicals, including hydroxyl radical, superoxide radical, peroxyl radical, DPPH radical and ABTS radicals, and performed more efficiently than that observed for BHT, Trolox and peptides from marine protein sources in most cases. The purified peptide also has significant protective effects on DNA and prevents cellular damage caused by hydroxyl radicals. In addition, the peptide has gastrointestinal enzyme-resistance and no cytotoxicity observed in human lung fibroblasts cell lines (WI-38)
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| subjects | Algae antioxidant activity Antioxidants - analysis Antioxidants - metabolism Antioxidative Applied sciences Biological and medical sciences Biotechnology - methods Biphenyl Compounds - chemistry Cell Cell Line, Tumor Chlorella vulgaris Chlorella vulgaris - metabolism Chromans - chemistry digestive enzymes DNA Damage Eukaryota - metabolism Exact sciences and technology Fibroblasts - drug effects Fundamental and applied biological sciences. Psychology gastrointestinal system Humans Hydroxyl Radical - chemistry Hydroxyl Radical - metabolism Inhibitory Concentration 50 microalgae oxidation Oxygen - chemistry pepsin Peptides Peptides - chemistry Picrates - chemistry Plant Extracts - pharmacology plant proteins Pollution proteinases Wastes |
| title | Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems |
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