Detection of zearalenone and its metabolites in naturally contaminated follicular fluids by using LC/MS/MS and in vitro effects of zearalenone on oocyte maturation in cattle
Zearalenone (Zen) and its metabolites are estrogenic and may be important factors involved in reproductive disorders in domestic animals. We aimed to (1) simultaneously detect Zen and its metabolites in bovine follicular fluids (FFs) by liquid chromatography–tandem mass spectrometry and (2) examine...
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| Veröffentlicht in: | Reproductive toxicology (Elmsford, N.Y.) N.Y.), 2008-10, Vol.26 (2), p.164-169 |
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| creator | Takagi, Mitsuhiro Mukai, Shuhei Kuriyagawa, Toshiyuki Takagaki, Katsuhito Uno, Seiichi Kokushi, Emiko Otoi, Takeshige Budiyanto, Agung Shirasuna, Koumei Miyamoto, Akio Kawamura, Osamu Okamoto, Koji Deguchi, Eisaburo |
| description | Zearalenone (Zen) and its metabolites are estrogenic and may be important factors involved in reproductive disorders in domestic animals. We aimed to (1) simultaneously detect Zen and its metabolites in bovine follicular fluids (FFs) by liquid chromatography–tandem mass spectrometry and (2) examine the in vitro effects of Zen on bovine oocytes. Zen and its metabolites were detected in 6 of 32 normal follicles and 7 of 20 cystic follicles. Bovine oocytes were cultured in a maturation media containing various Zen concentrations (0 [control], 1, 10, 100, and 1000
μg/L), fertilized, and cultured further. Maturation rates decreased dose-dependently. Further, maturation of 62 (50%) of 124 oocytes examined in the 1000-μg/L group was arrested in metaphase I, without affecting the fertilization rate. Blastocyst-formation rates did not significantly differ among the groups. Zen and its metabolites were detectable in bovine FFs. High Zen concentration may adversely affect meiotic competence but not the fertilization and development rates. |
| doi_str_mv | 10.1016/j.reprotox.2008.08.006 |
| format | Article |
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μg/L), fertilized, and cultured further. Maturation rates decreased dose-dependently. Further, maturation of 62 (50%) of 124 oocytes examined in the 1000-μg/L group was arrested in metaphase I, without affecting the fertilization rate. Blastocyst-formation rates did not significantly differ among the groups. Zen and its metabolites were detectable in bovine FFs. High Zen concentration may adversely affect meiotic competence but not the fertilization and development rates.</description><identifier>ISSN: 0890-6238</identifier><identifier>EISSN: 1873-1708</identifier><identifier>DOI: 10.1016/j.reprotox.2008.08.006</identifier><identifier>PMID: 18790045</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Cattle ; Chromatography, Liquid ; Embryology: invertebrates and vertebrates. Teratology ; Embryonic Development - drug effects ; Estrogens, Non-Steroidal - analysis ; Estrogens, Non-Steroidal - metabolism ; Estrogens, Non-Steroidal - toxicity ; Female ; Follicular fluid ; Follicular Fluid - chemistry ; Fundamental and applied biological sciences. Psychology ; In Vitro Techniques ; LC/MS/MS ; Medical sciences ; Oocyte maturation ; Oocytes - drug effects ; Plant poisons toxicology ; Tandem Mass Spectrometry ; Teratology. Teratogens ; Toxicology ; Zearalenone ; Zearalenone - analysis ; Zearalenone - metabolism ; Zearalenone - toxicity</subject><ispartof>Reproductive toxicology (Elmsford, N.Y.), 2008-10, Vol.26 (2), p.164-169</ispartof><rights>2008 Elsevier Inc.</rights><rights>2008 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-5a307e4efd26c45cec50f3dd9b9480c7fea1be6eaf7ef7c6454da01e9e8665383</citedby><cites>FETCH-LOGICAL-c493t-5a307e4efd26c45cec50f3dd9b9480c7fea1be6eaf7ef7c6454da01e9e8665383</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.reprotox.2008.08.006$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20798379$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18790045$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takagi, Mitsuhiro</creatorcontrib><creatorcontrib>Mukai, Shuhei</creatorcontrib><creatorcontrib>Kuriyagawa, Toshiyuki</creatorcontrib><creatorcontrib>Takagaki, Katsuhito</creatorcontrib><creatorcontrib>Uno, Seiichi</creatorcontrib><creatorcontrib>Kokushi, Emiko</creatorcontrib><creatorcontrib>Otoi, Takeshige</creatorcontrib><creatorcontrib>Budiyanto, Agung</creatorcontrib><creatorcontrib>Shirasuna, Koumei</creatorcontrib><creatorcontrib>Miyamoto, Akio</creatorcontrib><creatorcontrib>Kawamura, Osamu</creatorcontrib><creatorcontrib>Okamoto, Koji</creatorcontrib><creatorcontrib>Deguchi, Eisaburo</creatorcontrib><title>Detection of zearalenone and its metabolites in naturally contaminated follicular fluids by using LC/MS/MS and in vitro effects of zearalenone on oocyte maturation in cattle</title><title>Reproductive toxicology (Elmsford, N.Y.)</title><addtitle>Reprod Toxicol</addtitle><description>Zearalenone (Zen) and its metabolites are estrogenic and may be important factors involved in reproductive disorders in domestic animals. We aimed to (1) simultaneously detect Zen and its metabolites in bovine follicular fluids (FFs) by liquid chromatography–tandem mass spectrometry and (2) examine the in vitro effects of Zen on bovine oocytes. Zen and its metabolites were detected in 6 of 32 normal follicles and 7 of 20 cystic follicles. Bovine oocytes were cultured in a maturation media containing various Zen concentrations (0 [control], 1, 10, 100, and 1000
μg/L), fertilized, and cultured further. Maturation rates decreased dose-dependently. Further, maturation of 62 (50%) of 124 oocytes examined in the 1000-μg/L group was arrested in metaphase I, without affecting the fertilization rate. Blastocyst-formation rates did not significantly differ among the groups. Zen and its metabolites were detectable in bovine FFs. High Zen concentration may adversely affect meiotic competence but not the fertilization and development rates.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Chromatography, Liquid</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Embryonic Development - drug effects</subject><subject>Estrogens, Non-Steroidal - analysis</subject><subject>Estrogens, Non-Steroidal - metabolism</subject><subject>Estrogens, Non-Steroidal - toxicity</subject><subject>Female</subject><subject>Follicular fluid</subject><subject>Follicular Fluid - chemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>In Vitro Techniques</subject><subject>LC/MS/MS</subject><subject>Medical sciences</subject><subject>Oocyte maturation</subject><subject>Oocytes - drug effects</subject><subject>Plant poisons toxicology</subject><subject>Tandem Mass Spectrometry</subject><subject>Teratology. Teratogens</subject><subject>Toxicology</subject><subject>Zearalenone</subject><subject>Zearalenone - analysis</subject><subject>Zearalenone - metabolism</subject><subject>Zearalenone - toxicity</subject><issn>0890-6238</issn><issn>1873-1708</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-OFCEQxonRuOPqK2y46K1noZuG7ptm_JuM8aCeCQ2FYULDCvRmx3fyHaV3Rk28mFRCqPyqvi_1IXRFyZYSyq8P2wQ3KZZ4t20JGbZrEf4AbegguoYKMjxEGzKMpOFtN1ygJzkfCCFMjOIxuqjQWD_9Bv18DQV0cTHgaPEPUEl5CDEAVsFgVzKeoagpelcgYxdwUGWpjD9iHUNRs6sNMNhG751evErY-sWZjKcjXrIL3_B-d_3xc63TxoBvXUkRg7VVN_-ruvqI-lgAz_dC987qkFaleHiKHlnlMzw7v5fo69s3X3bvm_2ndx92r_aNZmNXml51RAADa1quWa9B98R2xozTyAaihQVFJ-CgrAArNGc9M4pQGGHgvO-G7hK9OO2tJ_6-QC5ydlmD9ypAXLJsKek5Y7SC_ATqFHNOYOVNcrNKR0mJXIOSB_k7KLkGJdcivA5enRWWaQbzd-ycTAWenwGVtfI2qaBd_sO1RIxDJ8bKvTxxUO9x6yDJrB0EDcalemBpovufl1_kCrpa</recordid><startdate>20081001</startdate><enddate>20081001</enddate><creator>Takagi, Mitsuhiro</creator><creator>Mukai, Shuhei</creator><creator>Kuriyagawa, Toshiyuki</creator><creator>Takagaki, Katsuhito</creator><creator>Uno, Seiichi</creator><creator>Kokushi, Emiko</creator><creator>Otoi, Takeshige</creator><creator>Budiyanto, Agung</creator><creator>Shirasuna, Koumei</creator><creator>Miyamoto, Akio</creator><creator>Kawamura, Osamu</creator><creator>Okamoto, Koji</creator><creator>Deguchi, Eisaburo</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20081001</creationdate><title>Detection of zearalenone and its metabolites in naturally contaminated follicular fluids by using LC/MS/MS and in vitro effects of zearalenone on oocyte maturation in cattle</title><author>Takagi, Mitsuhiro ; Mukai, Shuhei ; Kuriyagawa, Toshiyuki ; Takagaki, Katsuhito ; Uno, Seiichi ; Kokushi, Emiko ; Otoi, Takeshige ; Budiyanto, Agung ; Shirasuna, Koumei ; Miyamoto, Akio ; Kawamura, Osamu ; Okamoto, Koji ; Deguchi, Eisaburo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-5a307e4efd26c45cec50f3dd9b9480c7fea1be6eaf7ef7c6454da01e9e8665383</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Chromatography, Liquid</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Embryonic Development - drug effects</topic><topic>Estrogens, Non-Steroidal - analysis</topic><topic>Estrogens, Non-Steroidal - metabolism</topic><topic>Estrogens, Non-Steroidal - toxicity</topic><topic>Female</topic><topic>Follicular fluid</topic><topic>Follicular Fluid - chemistry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>In Vitro Techniques</topic><topic>LC/MS/MS</topic><topic>Medical sciences</topic><topic>Oocyte maturation</topic><topic>Oocytes - drug effects</topic><topic>Plant poisons toxicology</topic><topic>Tandem Mass Spectrometry</topic><topic>Teratology. 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We aimed to (1) simultaneously detect Zen and its metabolites in bovine follicular fluids (FFs) by liquid chromatography–tandem mass spectrometry and (2) examine the in vitro effects of Zen on bovine oocytes. Zen and its metabolites were detected in 6 of 32 normal follicles and 7 of 20 cystic follicles. Bovine oocytes were cultured in a maturation media containing various Zen concentrations (0 [control], 1, 10, 100, and 1000
μg/L), fertilized, and cultured further. Maturation rates decreased dose-dependently. Further, maturation of 62 (50%) of 124 oocytes examined in the 1000-μg/L group was arrested in metaphase I, without affecting the fertilization rate. Blastocyst-formation rates did not significantly differ among the groups. Zen and its metabolites were detectable in bovine FFs. High Zen concentration may adversely affect meiotic competence but not the fertilization and development rates.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>18790045</pmid><doi>10.1016/j.reprotox.2008.08.006</doi><tpages>6</tpages></addata></record> |
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| ispartof | Reproductive toxicology (Elmsford, N.Y.), 2008-10, Vol.26 (2), p.164-169 |
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| subjects | Animals Biological and medical sciences Cattle Chromatography, Liquid Embryology: invertebrates and vertebrates. Teratology Embryonic Development - drug effects Estrogens, Non-Steroidal - analysis Estrogens, Non-Steroidal - metabolism Estrogens, Non-Steroidal - toxicity Female Follicular fluid Follicular Fluid - chemistry Fundamental and applied biological sciences. Psychology In Vitro Techniques LC/MS/MS Medical sciences Oocyte maturation Oocytes - drug effects Plant poisons toxicology Tandem Mass Spectrometry Teratology. Teratogens Toxicology Zearalenone Zearalenone - analysis Zearalenone - metabolism Zearalenone - toxicity |
| title | Detection of zearalenone and its metabolites in naturally contaminated follicular fluids by using LC/MS/MS and in vitro effects of zearalenone on oocyte maturation in cattle |
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