Incorporation of Collagen from Marine Sponges (Spongin) into Hydroxyapatite Samples: Characterization and In Vitro Biological Evaluation
Biomaterial-based bone grafts have an important role in the field of bone tissue engineering. One of the most promising classes of biomaterials is collagen, including the ones from marine biodiversity (in general, called spongin (SPG)). Also, hydroxyapatite (HA) has an important role in stimulating...
Gespeichert in:
| Veröffentlicht in: | Marine biotechnology (New York, N.Y.) N.Y.), 2019-02, Vol.21 (1), p.30-37 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 37 |
|---|---|
| container_issue | 1 |
| container_start_page | 30 |
| container_title | Marine biotechnology (New York, N.Y.) |
| container_volume | 21 |
| creator | Parisi, J. R. Fernandes, K. R. Avanzi, I. R. Dorileo, B. P. Santana, A. F. Andrade, A. L. Gabbai-Armelin, P. R. Fortulan, C. A. Trichês, E. S. Granito, R. N. Renno, A. C. M. |
| description | Biomaterial-based bone grafts have an important role in the field of bone tissue engineering. One of the most promising classes of biomaterials is collagen, including the ones from marine biodiversity (in general, called spongin (SPG)). Also, hydroxyapatite (HA) has an important role in stimulating bone metabolism. Therefore, this work investigated the association of HA and SPG composites in order to evaluate their physico-chemical and morphological characteristics and their in vitro biological performance. For this, pre-set composite disks were evaluated by means of mass loss after incubation, pH, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and “in vitro” cell viability. pH measurements showed no statistical difference between groups. Moreover, a higher mass loss was observed for HA/SPG70/30 compared to the other groups for all experimental periods. Moreover, SEM representative micrographs showed the degradation of the samples with and without immersion. FTIR analysis demonstrated the absorption peaks for poly(methyl methacrylate) (PMMA), HA, and SPG. A higher L292 cell viability for control and PMMA was observed compared to HA and HA/SPG 90/10. Also, HA/SPG 70/30 showed higher cell viability compared to HA and HA/SPG 90/10 on days 3 and 7 days of culture. Furthermore, HA showed a significant lower MC3T3 cell viability compared to control and HA/SPG 70/30 on day 3 and no significant difference was observed between the composites in the last experimental period. Based on our investigations, it can be concluded that the mentioned composites were successfully obtained, presenting improved biological properties, especially the one mimicking the composition of bone (with 70% of HA and 30% of SPG). Consequently, these data highlight the potential of the introduction of SPG into HA to improve the performance of the graft for bone regeneration applications. Further long-term studies should be carried out to provide additional information concerning the late stages of material degradation and bone healing in the presence of HA/SPG. |
| doi_str_mv | 10.1007/s10126-018-9855-z |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2105057311</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2105057311</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-b1db7b49c8b1870afa23eb64e2a4d1c77fe3112adc2bfbbb5cef6abc21861cec3</originalsourceid><addsrcrecordid>eNp1kctO6zAQhi0EAg7wAGyQJTY9i4AvzaXsoIJDJRALLltr7DjFKLGDnSDaJ-CxcQkHJCRWM9J8_z8e_wjtU3JECcmPAyWUZQmhRTIp0jRZrqFtOuZZwhjP1r96VmyhPyE8kajJOdlEW5wwWnCWbaO3mVXOt85DZ5zFrsJTV9cw1xZX3jX4GryxGt-2zs51wKOPxti_2NjO4ctF6d3rAtqo7iIFTVvrcIKnj-BBddqb5eALtsQzix9M5x0-M652c6OgxucvUPcfyC7aqKAOeu-z7qD7i_O76WVydfNvNj29ShTPWZdIWspcjieqkLTICVTAuJbZWDMYl1TleaU5pQxKxWQlpUyVrjKQKt6bUaUV30Gjwbf17rnXoRONCUrHm612fRCMkpSkeTSJ6OEP9Mn13sbXrSg-ifvJJFJ0oJR3IXhdidabBvxCUCJWMYkhJhFjEquYxDJqDj6de9no8kvxP5cIsAEIcRR_3n-v_t31HfgZoN8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2103987009</pqid></control><display><type>article</type><title>Incorporation of Collagen from Marine Sponges (Spongin) into Hydroxyapatite Samples: Characterization and In Vitro Biological Evaluation</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Parisi, J. R. ; Fernandes, K. R. ; Avanzi, I. R. ; Dorileo, B. P. ; Santana, A. F. ; Andrade, A. L. ; Gabbai-Armelin, P. R. ; Fortulan, C. A. ; Trichês, E. S. ; Granito, R. N. ; Renno, A. C. M.</creator><creatorcontrib>Parisi, J. R. ; Fernandes, K. R. ; Avanzi, I. R. ; Dorileo, B. P. ; Santana, A. F. ; Andrade, A. L. ; Gabbai-Armelin, P. R. ; Fortulan, C. A. ; Trichês, E. S. ; Granito, R. N. ; Renno, A. C. M.</creatorcontrib><description>Biomaterial-based bone grafts have an important role in the field of bone tissue engineering. One of the most promising classes of biomaterials is collagen, including the ones from marine biodiversity (in general, called spongin (SPG)). Also, hydroxyapatite (HA) has an important role in stimulating bone metabolism. Therefore, this work investigated the association of HA and SPG composites in order to evaluate their physico-chemical and morphological characteristics and their in vitro biological performance. For this, pre-set composite disks were evaluated by means of mass loss after incubation, pH, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and “in vitro” cell viability. pH measurements showed no statistical difference between groups. Moreover, a higher mass loss was observed for HA/SPG70/30 compared to the other groups for all experimental periods. Moreover, SEM representative micrographs showed the degradation of the samples with and without immersion. FTIR analysis demonstrated the absorption peaks for poly(methyl methacrylate) (PMMA), HA, and SPG. A higher L292 cell viability for control and PMMA was observed compared to HA and HA/SPG 90/10. Also, HA/SPG 70/30 showed higher cell viability compared to HA and HA/SPG 90/10 on days 3 and 7 days of culture. Furthermore, HA showed a significant lower MC3T3 cell viability compared to control and HA/SPG 70/30 on day 3 and no significant difference was observed between the composites in the last experimental period. Based on our investigations, it can be concluded that the mentioned composites were successfully obtained, presenting improved biological properties, especially the one mimicking the composition of bone (with 70% of HA and 30% of SPG). Consequently, these data highlight the potential of the introduction of SPG into HA to improve the performance of the graft for bone regeneration applications. Further long-term studies should be carried out to provide additional information concerning the late stages of material degradation and bone healing in the presence of HA/SPG.</description><identifier>ISSN: 1436-2228</identifier><identifier>EISSN: 1436-2236</identifier><identifier>DOI: 10.1007/s10126-018-9855-z</identifier><identifier>PMID: 30218326</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Analytical methods ; Animals ; Biocompatible Materials - chemistry ; Biocompatible Materials - pharmacology ; Biodiversity ; Biological properties ; Biological sampling ; Biomaterials ; Biomedical and Life Sciences ; Biomedical materials ; Bone and Bones - cytology ; Bone biomaterials ; Bone composition ; Bone grafts ; Bone growth ; Bone healing ; Bone Substitutes - chemistry ; Bone Substitutes - pharmacology ; Bone turnover ; Bones ; Cell culture ; Cell Line ; Cell Survival - drug effects ; Collagen ; Collagen - chemistry ; Collagen - pharmacology ; Composite materials ; Degradation ; Disks ; Durapatite - chemistry ; Durapatite - pharmacology ; Electron microscopy ; Engineering ; Evaluation ; Fibroblasts - cytology ; Fibroblasts - drug effects ; Fourier transforms ; Freshwater & Marine Ecology ; Grafts ; Hydrogen-Ion Concentration ; Hydroxyapatite ; Incubation period ; Infrared spectroscopy ; Life Sciences ; Long bone ; Marine invertebrates ; Metabolism ; Mice ; Microbiology ; Micrography ; Mimicry ; NIH 3T3 Cells ; Organic chemistry ; Original Article ; Performance enhancement ; pH effects ; Photomicrographs ; Physical characteristics ; Polymethyl methacrylate ; Polymethyl Methacrylate - chemistry ; Polymethyl Methacrylate - pharmacology ; Polymethylmethacrylate ; Porifera - chemistry ; Regeneration ; Regeneration (biological) ; Regeneration (physiology) ; Scanning electron microscopy ; Statistical methods ; Submerging ; Substitute bone ; Surgical implants ; Tissue ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds ; Zoology</subject><ispartof>Marine biotechnology (New York, N.Y.), 2019-02, Vol.21 (1), p.30-37</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Marine Biotechnology is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-b1db7b49c8b1870afa23eb64e2a4d1c77fe3112adc2bfbbb5cef6abc21861cec3</citedby><cites>FETCH-LOGICAL-c372t-b1db7b49c8b1870afa23eb64e2a4d1c77fe3112adc2bfbbb5cef6abc21861cec3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10126-018-9855-z$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10126-018-9855-z$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30218326$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Parisi, J. R.</creatorcontrib><creatorcontrib>Fernandes, K. R.</creatorcontrib><creatorcontrib>Avanzi, I. R.</creatorcontrib><creatorcontrib>Dorileo, B. P.</creatorcontrib><creatorcontrib>Santana, A. F.</creatorcontrib><creatorcontrib>Andrade, A. L.</creatorcontrib><creatorcontrib>Gabbai-Armelin, P. R.</creatorcontrib><creatorcontrib>Fortulan, C. A.</creatorcontrib><creatorcontrib>Trichês, E. S.</creatorcontrib><creatorcontrib>Granito, R. N.</creatorcontrib><creatorcontrib>Renno, A. C. M.</creatorcontrib><title>Incorporation of Collagen from Marine Sponges (Spongin) into Hydroxyapatite Samples: Characterization and In Vitro Biological Evaluation</title><title>Marine biotechnology (New York, N.Y.)</title><addtitle>Mar Biotechnol</addtitle><addtitle>Mar Biotechnol (NY)</addtitle><description>Biomaterial-based bone grafts have an important role in the field of bone tissue engineering. One of the most promising classes of biomaterials is collagen, including the ones from marine biodiversity (in general, called spongin (SPG)). Also, hydroxyapatite (HA) has an important role in stimulating bone metabolism. Therefore, this work investigated the association of HA and SPG composites in order to evaluate their physico-chemical and morphological characteristics and their in vitro biological performance. For this, pre-set composite disks were evaluated by means of mass loss after incubation, pH, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and “in vitro” cell viability. pH measurements showed no statistical difference between groups. Moreover, a higher mass loss was observed for HA/SPG70/30 compared to the other groups for all experimental periods. Moreover, SEM representative micrographs showed the degradation of the samples with and without immersion. FTIR analysis demonstrated the absorption peaks for poly(methyl methacrylate) (PMMA), HA, and SPG. A higher L292 cell viability for control and PMMA was observed compared to HA and HA/SPG 90/10. Also, HA/SPG 70/30 showed higher cell viability compared to HA and HA/SPG 90/10 on days 3 and 7 days of culture. Furthermore, HA showed a significant lower MC3T3 cell viability compared to control and HA/SPG 70/30 on day 3 and no significant difference was observed between the composites in the last experimental period. Based on our investigations, it can be concluded that the mentioned composites were successfully obtained, presenting improved biological properties, especially the one mimicking the composition of bone (with 70% of HA and 30% of SPG). Consequently, these data highlight the potential of the introduction of SPG into HA to improve the performance of the graft for bone regeneration applications. Further long-term studies should be carried out to provide additional information concerning the late stages of material degradation and bone healing in the presence of HA/SPG.</description><subject>Analytical methods</subject><subject>Animals</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biocompatible Materials - pharmacology</subject><subject>Biodiversity</subject><subject>Biological properties</subject><subject>Biological sampling</subject><subject>Biomaterials</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedical materials</subject><subject>Bone and Bones - cytology</subject><subject>Bone biomaterials</subject><subject>Bone composition</subject><subject>Bone grafts</subject><subject>Bone growth</subject><subject>Bone healing</subject><subject>Bone Substitutes - chemistry</subject><subject>Bone Substitutes - pharmacology</subject><subject>Bone turnover</subject><subject>Bones</subject><subject>Cell culture</subject><subject>Cell Line</subject><subject>Cell Survival - drug effects</subject><subject>Collagen</subject><subject>Collagen - chemistry</subject><subject>Collagen - pharmacology</subject><subject>Composite materials</subject><subject>Degradation</subject><subject>Disks</subject><subject>Durapatite - chemistry</subject><subject>Durapatite - pharmacology</subject><subject>Electron microscopy</subject><subject>Engineering</subject><subject>Evaluation</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - drug effects</subject><subject>Fourier transforms</subject><subject>Freshwater & Marine Ecology</subject><subject>Grafts</subject><subject>Hydrogen-Ion Concentration</subject><subject>Hydroxyapatite</subject><subject>Incubation period</subject><subject>Infrared spectroscopy</subject><subject>Life Sciences</subject><subject>Long bone</subject><subject>Marine invertebrates</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Micrography</subject><subject>Mimicry</subject><subject>NIH 3T3 Cells</subject><subject>Organic chemistry</subject><subject>Original Article</subject><subject>Performance enhancement</subject><subject>pH effects</subject><subject>Photomicrographs</subject><subject>Physical characteristics</subject><subject>Polymethyl methacrylate</subject><subject>Polymethyl Methacrylate - chemistry</subject><subject>Polymethyl Methacrylate - pharmacology</subject><subject>Polymethylmethacrylate</subject><subject>Porifera - chemistry</subject><subject>Regeneration</subject><subject>Regeneration (biological)</subject><subject>Regeneration (physiology)</subject><subject>Scanning electron microscopy</subject><subject>Statistical methods</subject><subject>Submerging</subject><subject>Substitute bone</subject><subject>Surgical implants</subject><subject>Tissue</subject><subject>Tissue engineering</subject><subject>Tissue Engineering - methods</subject><subject>Tissue Scaffolds</subject><subject>Zoology</subject><issn>1436-2228</issn><issn>1436-2236</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kctO6zAQhi0EAg7wAGyQJTY9i4AvzaXsoIJDJRALLltr7DjFKLGDnSDaJ-CxcQkHJCRWM9J8_z8e_wjtU3JECcmPAyWUZQmhRTIp0jRZrqFtOuZZwhjP1r96VmyhPyE8kajJOdlEW5wwWnCWbaO3mVXOt85DZ5zFrsJTV9cw1xZX3jX4GryxGt-2zs51wKOPxti_2NjO4ctF6d3rAtqo7iIFTVvrcIKnj-BBddqb5eALtsQzix9M5x0-M652c6OgxucvUPcfyC7aqKAOeu-z7qD7i_O76WVydfNvNj29ShTPWZdIWspcjieqkLTICVTAuJbZWDMYl1TleaU5pQxKxWQlpUyVrjKQKt6bUaUV30Gjwbf17rnXoRONCUrHm612fRCMkpSkeTSJ6OEP9Mn13sbXrSg-ifvJJFJ0oJR3IXhdidabBvxCUCJWMYkhJhFjEquYxDJqDj6de9no8kvxP5cIsAEIcRR_3n-v_t31HfgZoN8</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Parisi, J. R.</creator><creator>Fernandes, K. R.</creator><creator>Avanzi, I. R.</creator><creator>Dorileo, B. P.</creator><creator>Santana, A. F.</creator><creator>Andrade, A. L.</creator><creator>Gabbai-Armelin, P. R.</creator><creator>Fortulan, C. A.</creator><creator>Trichês, E. S.</creator><creator>Granito, R. N.</creator><creator>Renno, A. C. M.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QO</scope><scope>7TN</scope><scope>7U9</scope><scope>7WY</scope><scope>7WZ</scope><scope>7X7</scope><scope>7XB</scope><scope>87Z</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8FL</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BEZIV</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>BKSAR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FRNLG</scope><scope>FYUFA</scope><scope>F~G</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>H95</scope><scope>H98</scope><scope>H99</scope><scope>HCIFZ</scope><scope>K60</scope><scope>K6~</scope><scope>K9.</scope><scope>L.-</scope><scope>L.F</scope><scope>L.G</scope><scope>L6V</scope><scope>LK8</scope><scope>M0C</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PCBAR</scope><scope>PQBIZ</scope><scope>PQBZA</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20190201</creationdate><title>Incorporation of Collagen from Marine Sponges (Spongin) into Hydroxyapatite Samples: Characterization and In Vitro Biological Evaluation</title><author>Parisi, J. R. ; Fernandes, K. R. ; Avanzi, I. R. ; Dorileo, B. P. ; Santana, A. F. ; Andrade, A. L. ; Gabbai-Armelin, P. R. ; Fortulan, C. A. ; Trichês, E. S. ; Granito, R. N. ; Renno, A. C. M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-b1db7b49c8b1870afa23eb64e2a4d1c77fe3112adc2bfbbb5cef6abc21861cec3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Analytical methods</topic><topic>Animals</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biocompatible Materials - pharmacology</topic><topic>Biodiversity</topic><topic>Biological properties</topic><topic>Biological sampling</topic><topic>Biomaterials</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedical materials</topic><topic>Bone and Bones - cytology</topic><topic>Bone biomaterials</topic><topic>Bone composition</topic><topic>Bone grafts</topic><topic>Bone growth</topic><topic>Bone healing</topic><topic>Bone Substitutes - chemistry</topic><topic>Bone Substitutes - pharmacology</topic><topic>Bone turnover</topic><topic>Bones</topic><topic>Cell culture</topic><topic>Cell Line</topic><topic>Cell Survival - drug effects</topic><topic>Collagen</topic><topic>Collagen - chemistry</topic><topic>Collagen - pharmacology</topic><topic>Composite materials</topic><topic>Degradation</topic><topic>Disks</topic><topic>Durapatite - chemistry</topic><topic>Durapatite - pharmacology</topic><topic>Electron microscopy</topic><topic>Engineering</topic><topic>Evaluation</topic><topic>Fibroblasts - cytology</topic><topic>Fibroblasts - drug effects</topic><topic>Fourier transforms</topic><topic>Freshwater & Marine Ecology</topic><topic>Grafts</topic><topic>Hydrogen-Ion Concentration</topic><topic>Hydroxyapatite</topic><topic>Incubation period</topic><topic>Infrared spectroscopy</topic><topic>Life Sciences</topic><topic>Long bone</topic><topic>Marine invertebrates</topic><topic>Metabolism</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Micrography</topic><topic>Mimicry</topic><topic>NIH 3T3 Cells</topic><topic>Organic chemistry</topic><topic>Original Article</topic><topic>Performance enhancement</topic><topic>pH effects</topic><topic>Photomicrographs</topic><topic>Physical characteristics</topic><topic>Polymethyl methacrylate</topic><topic>Polymethyl Methacrylate - chemistry</topic><topic>Polymethyl Methacrylate - pharmacology</topic><topic>Polymethylmethacrylate</topic><topic>Porifera - chemistry</topic><topic>Regeneration</topic><topic>Regeneration (biological)</topic><topic>Regeneration (physiology)</topic><topic>Scanning electron microscopy</topic><topic>Statistical methods</topic><topic>Submerging</topic><topic>Substitute bone</topic><topic>Surgical implants</topic><topic>Tissue</topic><topic>Tissue engineering</topic><topic>Tissue Engineering - methods</topic><topic>Tissue Scaffolds</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Parisi, J. R.</creatorcontrib><creatorcontrib>Fernandes, K. R.</creatorcontrib><creatorcontrib>Avanzi, I. R.</creatorcontrib><creatorcontrib>Dorileo, B. P.</creatorcontrib><creatorcontrib>Santana, A. F.</creatorcontrib><creatorcontrib>Andrade, A. L.</creatorcontrib><creatorcontrib>Gabbai-Armelin, P. R.</creatorcontrib><creatorcontrib>Fortulan, C. A.</creatorcontrib><creatorcontrib>Trichês, E. S.</creatorcontrib><creatorcontrib>Granito, R. N.</creatorcontrib><creatorcontrib>Renno, A. C. M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Oceanic Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Access via ABI/INFORM (ProQuest)</collection><collection>ABI/INFORM Global (PDF only)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ABI/INFORM Global (Alumni Edition)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ABI/INFORM Collection (Alumni Edition)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Business Premium Collection</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Earth, Atmospheric & Aquatic Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Business Premium Collection (Alumni)</collection><collection>Health Research Premium Collection</collection><collection>ABI/INFORM Global (Corporate)</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Aquaculture Abstracts</collection><collection>ASFA: Marine Biotechnology Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Business Collection (Alumni Edition)</collection><collection>ProQuest Business Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ABI/INFORM Professional Advanced</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Marine Biotechnology Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>ABI/INFORM Global</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Earth, Atmospheric & Aquatic Science Database</collection><collection>ProQuest One Business</collection><collection>ProQuest One Business (Alumni)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Marine biotechnology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Parisi, J. R.</au><au>Fernandes, K. R.</au><au>Avanzi, I. R.</au><au>Dorileo, B. P.</au><au>Santana, A. F.</au><au>Andrade, A. L.</au><au>Gabbai-Armelin, P. R.</au><au>Fortulan, C. A.</au><au>Trichês, E. S.</au><au>Granito, R. N.</au><au>Renno, A. C. M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Incorporation of Collagen from Marine Sponges (Spongin) into Hydroxyapatite Samples: Characterization and In Vitro Biological Evaluation</atitle><jtitle>Marine biotechnology (New York, N.Y.)</jtitle><stitle>Mar Biotechnol</stitle><addtitle>Mar Biotechnol (NY)</addtitle><date>2019-02-01</date><risdate>2019</risdate><volume>21</volume><issue>1</issue><spage>30</spage><epage>37</epage><pages>30-37</pages><issn>1436-2228</issn><eissn>1436-2236</eissn><abstract>Biomaterial-based bone grafts have an important role in the field of bone tissue engineering. One of the most promising classes of biomaterials is collagen, including the ones from marine biodiversity (in general, called spongin (SPG)). Also, hydroxyapatite (HA) has an important role in stimulating bone metabolism. Therefore, this work investigated the association of HA and SPG composites in order to evaluate their physico-chemical and morphological characteristics and their in vitro biological performance. For this, pre-set composite disks were evaluated by means of mass loss after incubation, pH, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and “in vitro” cell viability. pH measurements showed no statistical difference between groups. Moreover, a higher mass loss was observed for HA/SPG70/30 compared to the other groups for all experimental periods. Moreover, SEM representative micrographs showed the degradation of the samples with and without immersion. FTIR analysis demonstrated the absorption peaks for poly(methyl methacrylate) (PMMA), HA, and SPG. A higher L292 cell viability for control and PMMA was observed compared to HA and HA/SPG 90/10. Also, HA/SPG 70/30 showed higher cell viability compared to HA and HA/SPG 90/10 on days 3 and 7 days of culture. Furthermore, HA showed a significant lower MC3T3 cell viability compared to control and HA/SPG 70/30 on day 3 and no significant difference was observed between the composites in the last experimental period. Based on our investigations, it can be concluded that the mentioned composites were successfully obtained, presenting improved biological properties, especially the one mimicking the composition of bone (with 70% of HA and 30% of SPG). Consequently, these data highlight the potential of the introduction of SPG into HA to improve the performance of the graft for bone regeneration applications. Further long-term studies should be carried out to provide additional information concerning the late stages of material degradation and bone healing in the presence of HA/SPG.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30218326</pmid><doi>10.1007/s10126-018-9855-z</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1436-2228 |
| ispartof | Marine biotechnology (New York, N.Y.), 2019-02, Vol.21 (1), p.30-37 |
| issn | 1436-2228 1436-2236 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2105057311 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Analytical methods Animals Biocompatible Materials - chemistry Biocompatible Materials - pharmacology Biodiversity Biological properties Biological sampling Biomaterials Biomedical and Life Sciences Biomedical materials Bone and Bones - cytology Bone biomaterials Bone composition Bone grafts Bone growth Bone healing Bone Substitutes - chemistry Bone Substitutes - pharmacology Bone turnover Bones Cell culture Cell Line Cell Survival - drug effects Collagen Collagen - chemistry Collagen - pharmacology Composite materials Degradation Disks Durapatite - chemistry Durapatite - pharmacology Electron microscopy Engineering Evaluation Fibroblasts - cytology Fibroblasts - drug effects Fourier transforms Freshwater & Marine Ecology Grafts Hydrogen-Ion Concentration Hydroxyapatite Incubation period Infrared spectroscopy Life Sciences Long bone Marine invertebrates Metabolism Mice Microbiology Micrography Mimicry NIH 3T3 Cells Organic chemistry Original Article Performance enhancement pH effects Photomicrographs Physical characteristics Polymethyl methacrylate Polymethyl Methacrylate - chemistry Polymethyl Methacrylate - pharmacology Polymethylmethacrylate Porifera - chemistry Regeneration Regeneration (biological) Regeneration (physiology) Scanning electron microscopy Statistical methods Submerging Substitute bone Surgical implants Tissue Tissue engineering Tissue Engineering - methods Tissue Scaffolds Zoology |
| title | Incorporation of Collagen from Marine Sponges (Spongin) into Hydroxyapatite Samples: Characterization and In Vitro Biological Evaluation |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T23%3A20%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Incorporation%20of%20Collagen%20from%20Marine%20Sponges%20(Spongin)%20into%20Hydroxyapatite%20Samples:%20Characterization%20and%20In%20Vitro%20Biological%20Evaluation&rft.jtitle=Marine%20biotechnology%20(New%20York,%20N.Y.)&rft.au=Parisi,%20J.%20R.&rft.date=2019-02-01&rft.volume=21&rft.issue=1&rft.spage=30&rft.epage=37&rft.pages=30-37&rft.issn=1436-2228&rft.eissn=1436-2236&rft_id=info:doi/10.1007/s10126-018-9855-z&rft_dat=%3Cproquest_cross%3E2105057311%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2103987009&rft_id=info:pmid/30218326&rfr_iscdi=true |