Haploid Genetic Screens in Human Cells Identify Host Factors Used by Pathogens

Loss-of-function genetic screens in model organisms have elucidated numerous biological processes, but the diploid genome of mammalian cells has precluded large-scale gene disruption. We used insertional mutagenesis to develop a screening method to generate null alleles in a human cell line haploid...

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Veröffentlicht in:	Science (American Association for the Advancement of Science) 2009-11, Vol.326 (5957), p.1231-1235
Hauptverfasser:	Carette, Jan E, Guimaraes, Carla P, Varadarajan, Malini, Park, Annie S, Wuethrich, Irene, Godarova, Alzbeta, Kotecki, Maciej, Cochran, Brent H, Spooner, Eric, Ploegh, Hidde L, Brummelkamp, Thijn R
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Diphosphate Ribose - metabolism ADP Ribose Transferases - metabolism ADP Ribose Transferases - toxicity Amino Acid Sequence Antigens, Bacterial - metabolism Antigens, Bacterial - toxicity B lymphocytes Bacterial Toxins - metabolism Bacterial Toxins - toxicity Biological and medical sciences Biosynthetic Pathways Carboxylic Ester Hydrolases Cell cycle Cell Line, Tumor Cell lines Cellular biology Classical genetics, quantitative genetics, hybrids Diphtheria Toxin - metabolism Diphtheria Toxin - toxicity Exotoxins - metabolism Exotoxins - toxicity Fundamental and applied biological sciences. Psychology Genes Genetic screening Genetic Testing Genetics of eukaryotes. Biological and molecular evolution Genomics Haploidy HeLa cells Histidine - analogs & derivatives Histidine - biosynthesis Host-Pathogen Interactions Human Human genetics Humans Influenza Influenza A Virus, H1N1 Subtype - pathogenicity Medical genetics Medical screening Methyltransferases Molecular Sequence Data Monosaccharide Transport Proteins - genetics Monosaccharide Transport Proteins - metabolism Mutagenesis, Insertional N-Acylneuraminate Cytidylyltransferase - genetics N-Acylneuraminate Cytidylyltransferase - metabolism Peptide Elongation Factor 2 - metabolism Proteins - chemistry Proteins - genetics Proteins - metabolism Pseudomonas aeruginosa Exotoxin A Research Article Toxins Virulence Factors - metabolism Virulence Factors - toxicity
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