Saturated free fatty acid, palmitic acid, induces apoptosis in fetal hepatocytes in culture
To investigate the effects of saturated free fatty acid, palmitic acid (PA), on hepatocytes, we administered PA to rat hepatocytes. We demonstrated that PA inhibited the cell growth as a dose- and time-dependent manner in rat hepatocytes. PA-induced morphological changes including swelling, membrane...
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| Veröffentlicht in: | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2005-04, Vol.56 (6), p.369-376 |
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| container_title | Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie |
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| creator | Ji, Jun Zhang, Li Wang, Ping Mu, Yi-Ming Zhu, Xiao-Yu Wu, Yuan-Yuan Yu, Huan Zhang, Bin Chen, Shu-Min Sun, Xi-Zuo |
| description | To investigate the effects of saturated free fatty acid, palmitic acid (PA), on hepatocytes, we administered PA to rat hepatocytes. We demonstrated that PA inhibited the cell growth as a dose- and time-dependent manner in rat hepatocytes. PA-induced morphological changes including swelling, membrane dissolution and formation of debris, and apoptosis with appearance of sub-G1 fraction determined by cell cycle analysis after treatment for 4 days. The level of Bcl-2 was slightly decreased, in contrast, the level of Bax elevated markedly, which resulted in a significant decrease of Bcl-2/Bax ratio after PA treatment on HepG2 cells. These findings demonstrated that PA induces cell death on hepatocytes, perhaps via mitochondria-mediated apoptosis. Furthermore, the present study indicates that PA's cell toxicity may play important roles in the transition from steatosis to steatohepatitis in human especially with obesity. |
| doi_str_mv | 10.1016/j.etp.2005.02.003 |
| format | Article |
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We demonstrated that PA inhibited the cell growth as a dose- and time-dependent manner in rat hepatocytes. PA-induced morphological changes including swelling, membrane dissolution and formation of debris, and apoptosis with appearance of sub-G1 fraction determined by cell cycle analysis after treatment for 4 days. The level of Bcl-2 was slightly decreased, in contrast, the level of Bax elevated markedly, which resulted in a significant decrease of Bcl-2/Bax ratio after PA treatment on HepG2 cells. These findings demonstrated that PA induces cell death on hepatocytes, perhaps via mitochondria-mediated apoptosis. Furthermore, the present study indicates that PA's cell toxicity may play important roles in the transition from steatosis to steatohepatitis in human especially with obesity.</description><identifier>ISSN: 0940-2993</identifier><identifier>EISSN: 1618-1433</identifier><identifier>DOI: 10.1016/j.etp.2005.02.003</identifier><identifier>PMID: 15945276</identifier><language>eng</language><publisher>Jena: Elsevier GmbH</publisher><subject>Animals ; Annexin A5 - metabolism ; Apoptosis ; Apoptosis - drug effects ; bcl-2-Associated X Protein ; Biological and medical sciences ; Cell Enlargement - drug effects ; Cell Separation ; Cell Survival - drug effects ; Cell toxicity ; Cell viability ; Cells, Cultured ; Dose-Response Relationship, Drug ; Fetus - cytology ; Flow Cytometry ; Hepatocytes ; Hepatocytes - drug effects ; Hepatocytes - metabolism ; Hepatocytes - pathology ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Palmatic acid ; Palmitic Acid - toxicity ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Proto-Oncogene Proteins c-bcl-2 - genetics ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Rats</subject><ispartof>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie, 2005-04, Vol.56 (6), p.369-376</ispartof><rights>2005 Elsevier GmbH</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c478t-9f22ce77f681c7c7116e8d11c2bf7b453aa9dfaaa034c823240ad1b9ba9441bb3</citedby><cites>FETCH-LOGICAL-c478t-9f22ce77f681c7c7116e8d11c2bf7b453aa9dfaaa034c823240ad1b9ba9441bb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0940299305000151$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16777806$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15945276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ji, Jun</creatorcontrib><creatorcontrib>Zhang, Li</creatorcontrib><creatorcontrib>Wang, Ping</creatorcontrib><creatorcontrib>Mu, Yi-Ming</creatorcontrib><creatorcontrib>Zhu, Xiao-Yu</creatorcontrib><creatorcontrib>Wu, Yuan-Yuan</creatorcontrib><creatorcontrib>Yu, Huan</creatorcontrib><creatorcontrib>Zhang, Bin</creatorcontrib><creatorcontrib>Chen, Shu-Min</creatorcontrib><creatorcontrib>Sun, Xi-Zuo</creatorcontrib><title>Saturated free fatty acid, palmitic acid, induces apoptosis in fetal hepatocytes in culture</title><title>Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie</title><addtitle>Exp Toxicol Pathol</addtitle><description>To investigate the effects of saturated free fatty acid, palmitic acid (PA), on hepatocytes, we administered PA to rat hepatocytes. We demonstrated that PA inhibited the cell growth as a dose- and time-dependent manner in rat hepatocytes. PA-induced morphological changes including swelling, membrane dissolution and formation of debris, and apoptosis with appearance of sub-G1 fraction determined by cell cycle analysis after treatment for 4 days. The level of Bcl-2 was slightly decreased, in contrast, the level of Bax elevated markedly, which resulted in a significant decrease of Bcl-2/Bax ratio after PA treatment on HepG2 cells. These findings demonstrated that PA induces cell death on hepatocytes, perhaps via mitochondria-mediated apoptosis. Furthermore, the present study indicates that PA's cell toxicity may play important roles in the transition from steatosis to steatohepatitis in human especially with obesity.</description><subject>Animals</subject><subject>Annexin A5 - metabolism</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>bcl-2-Associated X Protein</subject><subject>Biological and medical sciences</subject><subject>Cell Enlargement - drug effects</subject><subject>Cell Separation</subject><subject>Cell Survival - drug effects</subject><subject>Cell toxicity</subject><subject>Cell viability</subject><subject>Cells, Cultured</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fetus - cytology</subject><subject>Flow Cytometry</subject><subject>Hepatocytes</subject><subject>Hepatocytes - drug effects</subject><subject>Hepatocytes - metabolism</subject><subject>Hepatocytes - pathology</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Palmatic acid</subject><subject>Palmitic Acid - toxicity</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Proto-Oncogene Proteins c-bcl-2 - genetics</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Rats</subject><issn>0940-2993</issn><issn>1618-1433</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kD2P1DAQhi0E4vYOfgANSgPVJXhsJ45FdTrxJZ1EAVQU1sQeC6-ym2A7SPvvybKRrqMazeh5X40exl4Bb4BD927fUJkbwXnbcNFwLp-wHXTQ16CkfMp23CheC2PkFbvOec-54KaF5-wKWqNaobsd-_kNy5KwkK9CIqoClnKq0EV_W804HmKJblvj0S-OcoXzNJcpx7xeqkAFx-oXzVgmdyr07-iWcS2lF-xZwDHTy23esB8fP3y__1w_fP305f7uoXZK96U2QQhHWoeuB6edBuio9wBODEEPqpWIxgdE5FK5XkihOHoYzIBGKRgGecPeXnrnNP1eKBd7iNnROOKRpiVbAVyB1O0KwgV0aco5UbBzigdMJwvcno3avV2N2rNRy4Vdja6Z11v5MhzIPyY2hSvwZgMwOxxDwqOL-ZHrtNY9P3PvLxytKv5ESja7SEdHPiZyxfop_ueNv-0clE0</recordid><startdate>20050401</startdate><enddate>20050401</enddate><creator>Ji, Jun</creator><creator>Zhang, Li</creator><creator>Wang, Ping</creator><creator>Mu, Yi-Ming</creator><creator>Zhu, Xiao-Yu</creator><creator>Wu, Yuan-Yuan</creator><creator>Yu, Huan</creator><creator>Zhang, Bin</creator><creator>Chen, Shu-Min</creator><creator>Sun, Xi-Zuo</creator><general>Elsevier GmbH</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U7</scope><scope>C1K</scope></search><sort><creationdate>20050401</creationdate><title>Saturated free fatty acid, palmitic acid, induces apoptosis in fetal hepatocytes in culture</title><author>Ji, Jun ; Zhang, Li ; Wang, Ping ; Mu, Yi-Ming ; Zhu, Xiao-Yu ; Wu, Yuan-Yuan ; Yu, Huan ; Zhang, Bin ; Chen, Shu-Min ; Sun, Xi-Zuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c478t-9f22ce77f681c7c7116e8d11c2bf7b453aa9dfaaa034c823240ad1b9ba9441bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Annexin A5 - metabolism</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>bcl-2-Associated X Protein</topic><topic>Biological and medical sciences</topic><topic>Cell Enlargement - drug effects</topic><topic>Cell Separation</topic><topic>Cell Survival - drug effects</topic><topic>Cell toxicity</topic><topic>Cell viability</topic><topic>Cells, Cultured</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fetus - cytology</topic><topic>Flow Cytometry</topic><topic>Hepatocytes</topic><topic>Hepatocytes - drug effects</topic><topic>Hepatocytes - metabolism</topic><topic>Hepatocytes - pathology</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Palmatic acid</topic><topic>Palmitic Acid - toxicity</topic><topic>Pathology. 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| subjects | Animals Annexin A5 - metabolism Apoptosis Apoptosis - drug effects bcl-2-Associated X Protein Biological and medical sciences Cell Enlargement - drug effects Cell Separation Cell Survival - drug effects Cell toxicity Cell viability Cells, Cultured Dose-Response Relationship, Drug Fetus - cytology Flow Cytometry Hepatocytes Hepatocytes - drug effects Hepatocytes - metabolism Hepatocytes - pathology Investigative techniques, diagnostic techniques (general aspects) Medical sciences Palmatic acid Palmitic Acid - toxicity Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Proto-Oncogene Proteins c-bcl-2 - genetics Proto-Oncogene Proteins c-bcl-2 - metabolism Rats |
| title | Saturated free fatty acid, palmitic acid, induces apoptosis in fetal hepatocytes in culture |
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