In vitro study of haematoporphyrin monomethyl ether-mediated sonodynamic effects on C6 glioma cells
Objectives To study the cytotoxicity induced by haematoporphyrin monomethyl ether (HMME)-mediated sonodynamic therapy (SDT) on C6 glioma cells. Methods The potent photosensitizer HMME was used as the sensitizer. Rat C6 glioma cells were incubated with HMME (10 μg/mL) in the dark for 2 h and then sub...
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| Veröffentlicht in: | Neurological sciences 2008-09, Vol.29 (4), p.229-235 |
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| creator | Li, Jian-hua Song, Da-yong Xu, Yong-gang Huang, Zheng Yue, Wu |
| description | Objectives
To study the cytotoxicity induced by haematoporphyrin monomethyl ether (HMME)-mediated sonodynamic therapy (SDT) on C6 glioma cells.
Methods
The potent photosensitizer HMME was used as the sensitizer. Rat C6 glioma cells were incubated with HMME (10 μg/mL) in the dark for 2 h and then subjected to ultrasound treatment at 1.0 MHz and 0.5 W/cm
2
for 2 min. The growth inhibition rate at different time points after SDT was determined by MTT assay. The apoptotic rate and cell circle profiles were examined with flow cytometry. Fine structures were observed with transmission electron microscope (TEM). The sonodynamic effect on the glioma cells was also studied in the absence or presence of various reactive oxygen species (ROS) scavengers.
Results
The growth inhibition rate of C6 glioma cells after SDT significantly increased. SDT also increased the apoptosis and proliferation rate (APR). TEM examination showed the morphological features of apoptosis or necrosis. The addition of NaN
3
showed a strong protective effect again SDT.
Conclusions
Our data indicated that SDT could kill C6 glioma cells in vitro and possibility through induction of apoptosis and necrosis. Singlet oxygen (
1
O
2
) may play an important role in SDT. |
| doi_str_mv | 10.1007/s10072-008-0972-8 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_21039449</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1561318521</sourcerecordid><originalsourceid>FETCH-LOGICAL-c466t-bb97c21a5aa4ca74aed09793a5f2f57186f74faae076101891979987f18cfc023</originalsourceid><addsrcrecordid>eNp1kE9LxDAQxYMo7rr6AbxI8OCtmmnTJjnK4p-FBS96Dtk02e3SNjVphX57s2xhQfAyGXi_eTN5CN0CeQRC2FM41DQhhCdExIafoTnkgiQZZfx86oEzOkNXIewJIUAhu0Qz4BxILoo50qsW_1S9dzj0QzliZ_FOmUb1rnO-242-anHjWteYfjfWOFbjk8aUlepNiUNUyrFVTaWxsdboPmDX4mWBt3XlGoW1qetwjS6sqoO5md4F-np9-Vy-J-uPt9XyeZ1oWhR9stkIplNQuVJUK0aVKeOvRKZym9qcAS8so1YpQ1gBBLiAqArOLHBtNUmzBXo4-nbefQ8m9LKpwuEC1Ro3BJkCyQSlIoL3f8C9G3wbb4sMLwpGU4gQHCHtXQjeWNn5qlF-lEDkIXh5jF_G-OUhfsnjzN1kPGxiSKeJKe8IpEcgRKndGn_a_L_rL1GYkG4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>218667421</pqid></control><display><type>article</type><title>In vitro study of haematoporphyrin monomethyl ether-mediated sonodynamic effects on C6 glioma cells</title><source>MEDLINE</source><source>SpringerLink Journals</source><creator>Li, Jian-hua ; Song, Da-yong ; Xu, Yong-gang ; Huang, Zheng ; Yue, Wu</creator><creatorcontrib>Li, Jian-hua ; Song, Da-yong ; Xu, Yong-gang ; Huang, Zheng ; Yue, Wu</creatorcontrib><description>Objectives
To study the cytotoxicity induced by haematoporphyrin monomethyl ether (HMME)-mediated sonodynamic therapy (SDT) on C6 glioma cells.
Methods
The potent photosensitizer HMME was used as the sensitizer. Rat C6 glioma cells were incubated with HMME (10 μg/mL) in the dark for 2 h and then subjected to ultrasound treatment at 1.0 MHz and 0.5 W/cm
2
for 2 min. The growth inhibition rate at different time points after SDT was determined by MTT assay. The apoptotic rate and cell circle profiles were examined with flow cytometry. Fine structures were observed with transmission electron microscope (TEM). The sonodynamic effect on the glioma cells was also studied in the absence or presence of various reactive oxygen species (ROS) scavengers.
Results
The growth inhibition rate of C6 glioma cells after SDT significantly increased. SDT also increased the apoptosis and proliferation rate (APR). TEM examination showed the morphological features of apoptosis or necrosis. The addition of NaN
3
showed a strong protective effect again SDT.
Conclusions
Our data indicated that SDT could kill C6 glioma cells in vitro and possibility through induction of apoptosis and necrosis. Singlet oxygen (
1
O
2
) may play an important role in SDT.</description><identifier>ISSN: 1590-1874</identifier><identifier>EISSN: 1590-3478</identifier><identifier>DOI: 10.1007/s10072-008-0972-8</identifier><identifier>PMID: 18810596</identifier><language>eng</language><publisher>Milan: Springer Milan</publisher><subject>Animals ; Antineoplastic Agents - toxicity ; Apoptosis - drug effects ; Apoptosis - physiology ; Apoptosis - radiation effects ; Brain Neoplasms - pathology ; Brain Neoplasms - therapy ; Cell Line, Tumor ; Cell Proliferation - drug effects ; Cell Proliferation - radiation effects ; Combined Modality Therapy ; Flow Cytometry ; Free Radical Scavengers - pharmacology ; Glioma - pathology ; Glioma - therapy ; Hematoporphyrins - toxicity ; Medicine ; Medicine & Public Health ; Microscopy, Electron, Transmission ; Molecular Structure ; Neurology ; Neuroradiology ; Neurosciences ; Neurosurgery ; Original Article ; Oxidative Stress - drug effects ; Oxidative Stress - physiology ; Photosensitizing Agents - toxicity ; Psychiatry ; Rats ; Reactive Oxygen Species - metabolism ; Ultrasonic Therapy - instrumentation ; Ultrasonic Therapy - methods</subject><ispartof>Neurological sciences, 2008-09, Vol.29 (4), p.229-235</ispartof><rights>Springer-Verlag Italia 2008</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-bb97c21a5aa4ca74aed09793a5f2f57186f74faae076101891979987f18cfc023</citedby><cites>FETCH-LOGICAL-c466t-bb97c21a5aa4ca74aed09793a5f2f57186f74faae076101891979987f18cfc023</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s10072-008-0972-8$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s10072-008-0972-8$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18810596$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Jian-hua</creatorcontrib><creatorcontrib>Song, Da-yong</creatorcontrib><creatorcontrib>Xu, Yong-gang</creatorcontrib><creatorcontrib>Huang, Zheng</creatorcontrib><creatorcontrib>Yue, Wu</creatorcontrib><title>In vitro study of haematoporphyrin monomethyl ether-mediated sonodynamic effects on C6 glioma cells</title><title>Neurological sciences</title><addtitle>Neurol Sci</addtitle><addtitle>Neurol Sci</addtitle><description>Objectives
To study the cytotoxicity induced by haematoporphyrin monomethyl ether (HMME)-mediated sonodynamic therapy (SDT) on C6 glioma cells.
Methods
The potent photosensitizer HMME was used as the sensitizer. Rat C6 glioma cells were incubated with HMME (10 μg/mL) in the dark for 2 h and then subjected to ultrasound treatment at 1.0 MHz and 0.5 W/cm
2
for 2 min. The growth inhibition rate at different time points after SDT was determined by MTT assay. The apoptotic rate and cell circle profiles were examined with flow cytometry. Fine structures were observed with transmission electron microscope (TEM). The sonodynamic effect on the glioma cells was also studied in the absence or presence of various reactive oxygen species (ROS) scavengers.
Results
The growth inhibition rate of C6 glioma cells after SDT significantly increased. SDT also increased the apoptosis and proliferation rate (APR). TEM examination showed the morphological features of apoptosis or necrosis. The addition of NaN
3
showed a strong protective effect again SDT.
Conclusions
Our data indicated that SDT could kill C6 glioma cells in vitro and possibility through induction of apoptosis and necrosis. Singlet oxygen (
1
O
2
) may play an important role in SDT.</description><subject>Animals</subject><subject>Antineoplastic Agents - toxicity</subject><subject>Apoptosis - drug effects</subject><subject>Apoptosis - physiology</subject><subject>Apoptosis - radiation effects</subject><subject>Brain Neoplasms - pathology</subject><subject>Brain Neoplasms - therapy</subject><subject>Cell Line, Tumor</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Proliferation - radiation effects</subject><subject>Combined Modality Therapy</subject><subject>Flow Cytometry</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Glioma - pathology</subject><subject>Glioma - therapy</subject><subject>Hematoporphyrins - toxicity</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Microscopy, Electron, Transmission</subject><subject>Molecular Structure</subject><subject>Neurology</subject><subject>Neuroradiology</subject><subject>Neurosciences</subject><subject>Neurosurgery</subject><subject>Original Article</subject><subject>Oxidative Stress - drug effects</subject><subject>Oxidative Stress - physiology</subject><subject>Photosensitizing Agents - toxicity</subject><subject>Psychiatry</subject><subject>Rats</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>Ultrasonic Therapy - instrumentation</subject><subject>Ultrasonic Therapy - methods</subject><issn>1590-1874</issn><issn>1590-3478</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp1kE9LxDAQxYMo7rr6AbxI8OCtmmnTJjnK4p-FBS96Dtk02e3SNjVphX57s2xhQfAyGXi_eTN5CN0CeQRC2FM41DQhhCdExIafoTnkgiQZZfx86oEzOkNXIewJIUAhu0Qz4BxILoo50qsW_1S9dzj0QzliZ_FOmUb1rnO-242-anHjWteYfjfWOFbjk8aUlepNiUNUyrFVTaWxsdboPmDX4mWBt3XlGoW1qetwjS6sqoO5md4F-np9-Vy-J-uPt9XyeZ1oWhR9stkIplNQuVJUK0aVKeOvRKZym9qcAS8so1YpQ1gBBLiAqArOLHBtNUmzBXo4-nbefQ8m9LKpwuEC1Ro3BJkCyQSlIoL3f8C9G3wbb4sMLwpGU4gQHCHtXQjeWNn5qlF-lEDkIXh5jF_G-OUhfsnjzN1kPGxiSKeJKe8IpEcgRKndGn_a_L_rL1GYkG4</recordid><startdate>20080901</startdate><enddate>20080901</enddate><creator>Li, Jian-hua</creator><creator>Song, Da-yong</creator><creator>Xu, Yong-gang</creator><creator>Huang, Zheng</creator><creator>Yue, Wu</creator><general>Springer Milan</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88G</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>K9-</scope><scope>K9.</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>M2M</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PSYQQ</scope><scope>Q9U</scope></search><sort><creationdate>20080901</creationdate><title>In vitro study of haematoporphyrin monomethyl ether-mediated sonodynamic effects on C6 glioma cells</title><author>Li, Jian-hua ; Song, Da-yong ; Xu, Yong-gang ; Huang, Zheng ; Yue, Wu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-bb97c21a5aa4ca74aed09793a5f2f57186f74faae076101891979987f18cfc023</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antineoplastic Agents - toxicity</topic><topic>Apoptosis - drug effects</topic><topic>Apoptosis - physiology</topic><topic>Apoptosis - radiation effects</topic><topic>Brain Neoplasms - pathology</topic><topic>Brain Neoplasms - therapy</topic><topic>Cell Line, Tumor</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Proliferation - radiation effects</topic><topic>Combined Modality Therapy</topic><topic>Flow Cytometry</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Glioma - pathology</topic><topic>Glioma - therapy</topic><topic>Hematoporphyrins - toxicity</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Microscopy, Electron, Transmission</topic><topic>Molecular Structure</topic><topic>Neurology</topic><topic>Neuroradiology</topic><topic>Neurosciences</topic><topic>Neurosurgery</topic><topic>Original Article</topic><topic>Oxidative Stress - drug effects</topic><topic>Oxidative Stress - physiology</topic><topic>Photosensitizing Agents - toxicity</topic><topic>Psychiatry</topic><topic>Rats</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>Ultrasonic Therapy - instrumentation</topic><topic>Ultrasonic Therapy - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Jian-hua</creatorcontrib><creatorcontrib>Song, Da-yong</creatorcontrib><creatorcontrib>Xu, Yong-gang</creatorcontrib><creatorcontrib>Huang, Zheng</creatorcontrib><creatorcontrib>Yue, Wu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Psychology Database (Alumni)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Psychology</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest One Psychology</collection><collection>ProQuest Central Basic</collection><jtitle>Neurological sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Jian-hua</au><au>Song, Da-yong</au><au>Xu, Yong-gang</au><au>Huang, Zheng</au><au>Yue, Wu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro study of haematoporphyrin monomethyl ether-mediated sonodynamic effects on C6 glioma cells</atitle><jtitle>Neurological sciences</jtitle><stitle>Neurol Sci</stitle><addtitle>Neurol Sci</addtitle><date>2008-09-01</date><risdate>2008</risdate><volume>29</volume><issue>4</issue><spage>229</spage><epage>235</epage><pages>229-235</pages><issn>1590-1874</issn><eissn>1590-3478</eissn><abstract>Objectives
To study the cytotoxicity induced by haematoporphyrin monomethyl ether (HMME)-mediated sonodynamic therapy (SDT) on C6 glioma cells.
Methods
The potent photosensitizer HMME was used as the sensitizer. Rat C6 glioma cells were incubated with HMME (10 μg/mL) in the dark for 2 h and then subjected to ultrasound treatment at 1.0 MHz and 0.5 W/cm
2
for 2 min. The growth inhibition rate at different time points after SDT was determined by MTT assay. The apoptotic rate and cell circle profiles were examined with flow cytometry. Fine structures were observed with transmission electron microscope (TEM). The sonodynamic effect on the glioma cells was also studied in the absence or presence of various reactive oxygen species (ROS) scavengers.
Results
The growth inhibition rate of C6 glioma cells after SDT significantly increased. SDT also increased the apoptosis and proliferation rate (APR). TEM examination showed the morphological features of apoptosis or necrosis. The addition of NaN
3
showed a strong protective effect again SDT.
Conclusions
Our data indicated that SDT could kill C6 glioma cells in vitro and possibility through induction of apoptosis and necrosis. Singlet oxygen (
1
O
2
) may play an important role in SDT.</abstract><cop>Milan</cop><pub>Springer Milan</pub><pmid>18810596</pmid><doi>10.1007/s10072-008-0972-8</doi><tpages>7</tpages></addata></record> |
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| ispartof | Neurological sciences, 2008-09, Vol.29 (4), p.229-235 |
| issn | 1590-1874 1590-3478 |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Animals Antineoplastic Agents - toxicity Apoptosis - drug effects Apoptosis - physiology Apoptosis - radiation effects Brain Neoplasms - pathology Brain Neoplasms - therapy Cell Line, Tumor Cell Proliferation - drug effects Cell Proliferation - radiation effects Combined Modality Therapy Flow Cytometry Free Radical Scavengers - pharmacology Glioma - pathology Glioma - therapy Hematoporphyrins - toxicity Medicine Medicine & Public Health Microscopy, Electron, Transmission Molecular Structure Neurology Neuroradiology Neurosciences Neurosurgery Original Article Oxidative Stress - drug effects Oxidative Stress - physiology Photosensitizing Agents - toxicity Psychiatry Rats Reactive Oxygen Species - metabolism Ultrasonic Therapy - instrumentation Ultrasonic Therapy - methods |
| title | In vitro study of haematoporphyrin monomethyl ether-mediated sonodynamic effects on C6 glioma cells |
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