NADH Autofluorescence—A Marker on its Way to Boost Bioenergetic Research

More than 60 years ago, the idea was introduced that NADH autofluorescence could be used as a marker of cellular redox state and indirectly also of cellular energy metabolism. Fluorescence lifetime imaging microscopy of NADH autofluorescence offers a marker‐free readout of the mitochondrial function...

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Veröffentlicht in:	Cytometry. Part A 2019-01, Vol.95 (1), p.34-46
Hauptverfasser:	Schaefer, Patrick M., Kalinina, Sviatlana, Rueck, Angelika, von Arnim, Christine A.F., von Einem, Bjoern
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioenergetics Cytometry Energy metabolism Energy Metabolism - physiology FLIM Fluorescence Imaging In vivo methods and tests Metabolism Microscopy Microscopy, Fluorescence - methods Mitochondria Mitochondria - metabolism NAD - chemistry NADH Nicotinamide adenine dinucleotide Optical Imaging Oxidation-Reduction Redox properties Spatial discrimination Spatial resolution Spectrometry, Fluorescence
Online-Zugang:	Volltext
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