Zeolite X from coal fly ash inhibits proliferation of human breast cancer cell lines (MCF-7) via induction of S phase arrest and apoptosis
In this study, zeolite X synthesised from coal fly ash (CFA) was examined for its anti-proliferative effects on human breast cancer MCF-7 cells by in vitro studies and allied probable molecular mechanism were also assessed. MTT assay exposed that zeolite X showed a concentration-dependent inhibitory...
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| Veröffentlicht in: | Molecular biology reports 2018-12, Vol.45 (6), p.2063-2074 |
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| description | In this study, zeolite X synthesised from coal fly ash (CFA) was examined for its anti-proliferative effects on human breast cancer MCF-7 cells by in vitro studies and allied probable molecular mechanism were also assessed. MTT assay exposed that zeolite X showed a concentration-dependent inhibitory result on the proliferation of MCF-7 cells and caused early apoptotic death and does not induce cytotoxicity in non-cancerous cells (MCF-12A). Flow cytometric study specified the accrual of cells at S phase suggest induction of apoptosis which was avowed through fluorescence and confocal microscopy following annexin V-FITC/propidium iodide (PI). MCF-7 cells treated by 10, 15 and 20 µg/ml concentrations of zeolite X showed internucleosomal DNA fragments in ladder form, thereby indicates cell death is associated with apoptosis. Mechanistically, our data support the induction of apoptosis through the activation of mitochondrial dependent pathway as indicated by an up-regulation of Bax and downregulation of Bcl-2 ratio, the expulsion of cytochrome c from the mitochondria to the cytosol and cleavage of pro-caspase-3 into activation of caspase-3 in MCF-7 cells. Based on these outcomes zeolite X induced early apoptosis and strongly provided experimental evidence for the usage of zeolite X as an essential therapeutic agent in the prevention and therapy of human breast cancer. |
| doi_str_mv | 10.1007/s11033-018-4363-9 |
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MTT assay exposed that zeolite X showed a concentration-dependent inhibitory result on the proliferation of MCF-7 cells and caused early apoptotic death and does not induce cytotoxicity in non-cancerous cells (MCF-12A). Flow cytometric study specified the accrual of cells at S phase suggest induction of apoptosis which was avowed through fluorescence and confocal microscopy following annexin V-FITC/propidium iodide (PI). MCF-7 cells treated by 10, 15 and 20 µg/ml concentrations of zeolite X showed internucleosomal DNA fragments in ladder form, thereby indicates cell death is associated with apoptosis. Mechanistically, our data support the induction of apoptosis through the activation of mitochondrial dependent pathway as indicated by an up-regulation of Bax and downregulation of Bcl-2 ratio, the expulsion of cytochrome c from the mitochondria to the cytosol and cleavage of pro-caspase-3 into activation of caspase-3 in MCF-7 cells. Based on these outcomes zeolite X induced early apoptosis and strongly provided experimental evidence for the usage of zeolite X as an essential therapeutic agent in the prevention and therapy of human breast cancer.</description><identifier>ISSN: 0301-4851</identifier><identifier>EISSN: 1573-4978</identifier><identifier>DOI: 10.1007/s11033-018-4363-9</identifier><identifier>PMID: 30206739</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Animal Anatomy ; Animal Biochemistry ; Annexin V ; Apoptosis ; Apoptosis - drug effects ; Bcl-2 protein ; bcl-2-Associated X Protein ; Biomedical and Life Sciences ; Breast cancer ; Breast Neoplasms - metabolism ; Caspase ; Caspase 3 ; Cell Cycle Checkpoints - drug effects ; Cell death ; Cell proliferation ; Cell Proliferation - drug effects ; Cell Survival ; Coal Ash - pharmacology ; Confocal microscopy ; Cytochrome c ; Cytochromes c ; Cytosol ; Cytotoxicity ; Deoxyribonucleic acid ; DNA ; Female ; Flow cytometry ; Fly ash ; Histology ; Humans ; Life Sciences ; MCF-7 Cells - drug effects ; Membrane Potential, Mitochondrial ; Mitochondria ; Morphology ; Original Article ; Propidium iodide ; Proto-Oncogene Proteins c-bcl-2 ; S phase ; S Phase - drug effects ; Tumor cell lines ; Zeolites ; Zeolites - pharmacology</subject><ispartof>Molecular biology reports, 2018-12, Vol.45 (6), p.2063-2074</ispartof><rights>Springer Nature B.V. 2018</rights><rights>Molecular Biology Reports is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-679987d618bba719e0206306848696a930a74e64a7a94af95ef96cec1c3ee36e3</citedby><cites>FETCH-LOGICAL-c372t-679987d618bba719e0206306848696a930a74e64a7a94af95ef96cec1c3ee36e3</cites><orcidid>0000-0002-1178-1565 ; 0000-0001-7727-9992</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11033-018-4363-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11033-018-4363-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30206739$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Subhapriya, S.</creatorcontrib><creatorcontrib>Gomathipriya, P.</creatorcontrib><title>Zeolite X from coal fly ash inhibits proliferation of human breast cancer cell lines (MCF-7) via induction of S phase arrest and apoptosis</title><title>Molecular biology reports</title><addtitle>Mol Biol Rep</addtitle><addtitle>Mol Biol Rep</addtitle><description>In this study, zeolite X synthesised from coal fly ash (CFA) was examined for its anti-proliferative effects on human breast cancer MCF-7 cells by in vitro studies and allied probable molecular mechanism were also assessed. MTT assay exposed that zeolite X showed a concentration-dependent inhibitory result on the proliferation of MCF-7 cells and caused early apoptotic death and does not induce cytotoxicity in non-cancerous cells (MCF-12A). Flow cytometric study specified the accrual of cells at S phase suggest induction of apoptosis which was avowed through fluorescence and confocal microscopy following annexin V-FITC/propidium iodide (PI). MCF-7 cells treated by 10, 15 and 20 µg/ml concentrations of zeolite X showed internucleosomal DNA fragments in ladder form, thereby indicates cell death is associated with apoptosis. Mechanistically, our data support the induction of apoptosis through the activation of mitochondrial dependent pathway as indicated by an up-regulation of Bax and downregulation of Bcl-2 ratio, the expulsion of cytochrome c from the mitochondria to the cytosol and cleavage of pro-caspase-3 into activation of caspase-3 in MCF-7 cells. Based on these outcomes zeolite X induced early apoptosis and strongly provided experimental evidence for the usage of zeolite X as an essential therapeutic agent in the prevention and therapy of human breast cancer.</description><subject>Animal Anatomy</subject><subject>Animal Biochemistry</subject><subject>Annexin V</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Bcl-2 protein</subject><subject>bcl-2-Associated X Protein</subject><subject>Biomedical and Life Sciences</subject><subject>Breast cancer</subject><subject>Breast Neoplasms - metabolism</subject><subject>Caspase</subject><subject>Caspase 3</subject><subject>Cell Cycle Checkpoints - drug effects</subject><subject>Cell death</subject><subject>Cell proliferation</subject><subject>Cell Proliferation - drug effects</subject><subject>Cell Survival</subject><subject>Coal Ash - pharmacology</subject><subject>Confocal microscopy</subject><subject>Cytochrome c</subject><subject>Cytochromes c</subject><subject>Cytosol</subject><subject>Cytotoxicity</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Female</subject><subject>Flow cytometry</subject><subject>Fly ash</subject><subject>Histology</subject><subject>Humans</subject><subject>Life Sciences</subject><subject>MCF-7 Cells - drug effects</subject><subject>Membrane Potential, Mitochondrial</subject><subject>Mitochondria</subject><subject>Morphology</subject><subject>Original Article</subject><subject>Propidium iodide</subject><subject>Proto-Oncogene Proteins c-bcl-2</subject><subject>S phase</subject><subject>S Phase - drug effects</subject><subject>Tumor cell lines</subject><subject>Zeolites</subject><subject>Zeolites - pharmacology</subject><issn>0301-4851</issn><issn>1573-4978</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kUFv1DAQhS0EosvCD-CCRuJSDqZ2nLXjI1pRQCrqoSAhLtbEO2FdJXGwk0r9C_zqetkWJCROc_D33rzxY-ylFG-lEOYsSymU4kI2vFZacfuIreTGKF5b0zxmK6GE5HWzkSfsWc7XQohams1TdqJEJbRRdsV-fafYh5ngG3QpDuAj9tD1t4B5D2HchzbMGaZUoI4SziGOEDvYLwOO0CbCPIPH0VMCT30PfRgpw-nn7Tk3b-AmYDHZLf5BdwXTHjMBpkRFieMOcIrTHHPIz9mTDvtML-7nmn09f_9l-5FfXH74tH13wb0y1cy1sbYxOy2btkUjLR1uUUI3daOtRqsEmpp0jQZtjZ3dUGe1Jy-9IlKa1JqdHn3LVT-XEsMNIR_C40hxya6SorKVsuVv1-z1P-h1XNJY0v2mpJa6MYWSR8qnmHOizk0pDJhunRTuUJQ7FuVKUe5QlLNF8-reeWkH2v1RPDRTgOoI5PI0_qD0d_X_Xe8AKVWc_A</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Subhapriya, S.</creator><creator>Gomathipriya, P.</creator><general>Springer Netherlands</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-1178-1565</orcidid><orcidid>https://orcid.org/0000-0001-7727-9992</orcidid></search><sort><creationdate>20181201</creationdate><title>Zeolite X from coal fly ash inhibits proliferation of human breast cancer cell lines (MCF-7) via induction of S phase arrest and apoptosis</title><author>Subhapriya, S. ; Gomathipriya, P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-679987d618bba719e0206306848696a930a74e64a7a94af95ef96cec1c3ee36e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animal Anatomy</topic><topic>Animal Biochemistry</topic><topic>Annexin V</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Bcl-2 protein</topic><topic>bcl-2-Associated X Protein</topic><topic>Biomedical and Life Sciences</topic><topic>Breast cancer</topic><topic>Breast Neoplasms - metabolism</topic><topic>Caspase</topic><topic>Caspase 3</topic><topic>Cell Cycle Checkpoints - drug effects</topic><topic>Cell death</topic><topic>Cell proliferation</topic><topic>Cell Proliferation - drug effects</topic><topic>Cell Survival</topic><topic>Coal Ash - pharmacology</topic><topic>Confocal microscopy</topic><topic>Cytochrome c</topic><topic>Cytochromes c</topic><topic>Cytosol</topic><topic>Cytotoxicity</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Female</topic><topic>Flow cytometry</topic><topic>Fly ash</topic><topic>Histology</topic><topic>Humans</topic><topic>Life Sciences</topic><topic>MCF-7 Cells - drug effects</topic><topic>Membrane Potential, Mitochondrial</topic><topic>Mitochondria</topic><topic>Morphology</topic><topic>Original Article</topic><topic>Propidium iodide</topic><topic>Proto-Oncogene Proteins c-bcl-2</topic><topic>S phase</topic><topic>S Phase - drug effects</topic><topic>Tumor cell lines</topic><topic>Zeolites</topic><topic>Zeolites - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Subhapriya, S.</creatorcontrib><creatorcontrib>Gomathipriya, P.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular biology reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Subhapriya, S.</au><au>Gomathipriya, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Zeolite X from coal fly ash inhibits proliferation of human breast cancer cell lines (MCF-7) via induction of S phase arrest and apoptosis</atitle><jtitle>Molecular biology reports</jtitle><stitle>Mol Biol Rep</stitle><addtitle>Mol Biol Rep</addtitle><date>2018-12-01</date><risdate>2018</risdate><volume>45</volume><issue>6</issue><spage>2063</spage><epage>2074</epage><pages>2063-2074</pages><issn>0301-4851</issn><eissn>1573-4978</eissn><abstract>In this study, zeolite X synthesised from coal fly ash (CFA) was examined for its anti-proliferative effects on human breast cancer MCF-7 cells by in vitro studies and allied probable molecular mechanism were also assessed. MTT assay exposed that zeolite X showed a concentration-dependent inhibitory result on the proliferation of MCF-7 cells and caused early apoptotic death and does not induce cytotoxicity in non-cancerous cells (MCF-12A). Flow cytometric study specified the accrual of cells at S phase suggest induction of apoptosis which was avowed through fluorescence and confocal microscopy following annexin V-FITC/propidium iodide (PI). MCF-7 cells treated by 10, 15 and 20 µg/ml concentrations of zeolite X showed internucleosomal DNA fragments in ladder form, thereby indicates cell death is associated with apoptosis. Mechanistically, our data support the induction of apoptosis through the activation of mitochondrial dependent pathway as indicated by an up-regulation of Bax and downregulation of Bcl-2 ratio, the expulsion of cytochrome c from the mitochondria to the cytosol and cleavage of pro-caspase-3 into activation of caspase-3 in MCF-7 cells. Based on these outcomes zeolite X induced early apoptosis and strongly provided experimental evidence for the usage of zeolite X as an essential therapeutic agent in the prevention and therapy of human breast cancer.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30206739</pmid><doi>10.1007/s11033-018-4363-9</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0002-1178-1565</orcidid><orcidid>https://orcid.org/0000-0001-7727-9992</orcidid></addata></record> |
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| subjects | Animal Anatomy Animal Biochemistry Annexin V Apoptosis Apoptosis - drug effects Bcl-2 protein bcl-2-Associated X Protein Biomedical and Life Sciences Breast cancer Breast Neoplasms - metabolism Caspase Caspase 3 Cell Cycle Checkpoints - drug effects Cell death Cell proliferation Cell Proliferation - drug effects Cell Survival Coal Ash - pharmacology Confocal microscopy Cytochrome c Cytochromes c Cytosol Cytotoxicity Deoxyribonucleic acid DNA Female Flow cytometry Fly ash Histology Humans Life Sciences MCF-7 Cells - drug effects Membrane Potential, Mitochondrial Mitochondria Morphology Original Article Propidium iodide Proto-Oncogene Proteins c-bcl-2 S phase S Phase - drug effects Tumor cell lines Zeolites Zeolites - pharmacology |
| title | Zeolite X from coal fly ash inhibits proliferation of human breast cancer cell lines (MCF-7) via induction of S phase arrest and apoptosis |
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