Human tuberculosis brain promotes neuronal apoptosis but not in astrocytes with high expression of vascular endothelial growth factor
The present study aimed to investigate the involvement of the angiogenic marker vascular endothelia growth factor (VEGF) and apoptotic markers of Bcl-2 and Bax in the neurons and astrocytes in the brain infected by Mycobacterium tuberculosis. The immunohistochemistry staining was performed to analyz...
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| Veröffentlicht in: | Tuberculosis (Edinburgh, Scotland) Scotland), 2018-09, Vol.112, p.45-51 |
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| creator | Othman, Fatihah N. Muthuraju, Sangu Noor, Siti Suraiya M. Abdullah, Sarimah Mohd Yusoff, Abdul Aziz Tharakan, John Bhaskar, Shalini Mahmood, Mohd Shah Kassim, Fauziah Rafia, Hanip Mohd Haspani, Mohd Saffari Alias, Azmi Pando, Rogelio H. Abdullah, Jafri M. Jaafar, Hasnan |
| description | The present study aimed to investigate the involvement of the angiogenic marker vascular endothelia growth factor (VEGF) and apoptotic markers of Bcl-2 and Bax in the neurons and astrocytes in the brain infected by Mycobacterium tuberculosis. The immunohistochemistry staining was performed to analyze the expression of the VEGF, Bcl-2 and Bax in the astrocytes and neurons. The expression of VEGF was high in neurons and astrocytes in both the infected brain and control tissues with no difference of angiogenic activity (p = 0.40). Higher Bcl-2 expression was seen in astrocytes of infected brain tissues compared to the control tissues (p = 0.004) promoted a higher anti-apoptotic activity in astrocytes. The neurons expressed strong Bax expression in the infected brain tissues compared to the control tissues (p |
| doi_str_mv | 10.1016/j.tube.2018.07.007 |
| format | Article |
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The immunohistochemistry staining was performed to analyze the expression of the VEGF, Bcl-2 and Bax in the astrocytes and neurons. The expression of VEGF was high in neurons and astrocytes in both the infected brain and control tissues with no difference of angiogenic activity (p = 0.40). Higher Bcl-2 expression was seen in astrocytes of infected brain tissues compared to the control tissues (p = 0.004) promoted a higher anti-apoptotic activity in astrocytes. The neurons expressed strong Bax expression in the infected brain tissues compared to the control tissues (p < 0.001), which indicated more apoptosis in neurons. Thus, neuronal death and survival of infected astrocytes together with high expression of VEGF might be associated with formation of brain tuberculosis. In conclusion, neurons could be more vulnerable than astrocytes in human tuberculosis brain with high expression of VEGF.</description><identifier>ISSN: 1472-9792</identifier><identifier>EISSN: 1873-281X</identifier><identifier>DOI: 10.1016/j.tube.2018.07.007</identifier><identifier>PMID: 30205968</identifier><language>eng</language><publisher>Scotland: Elsevier Ltd</publisher><subject>Angiogenesis ; Apoptosis ; Astrocyte ; Astrocytes ; Astrocytes - metabolism ; Astrocytes - microbiology ; Astrocytes - pathology ; Bacteria ; Bcl-2 protein ; bcl-2-Associated X Protein - metabolism ; Bcl2 and Bax ; Brain ; Case-Control Studies ; Growth factors ; Human brain tuberculosis ; Humans ; Immunohistochemistry ; Mycobacterium tuberculosis - pathogenicity ; Neurons ; Neurons - metabolism ; Neurons - microbiology ; Neurons - pathology ; Proto-Oncogene Proteins c-bcl-2 - metabolism ; Signal Transduction ; Tissues ; Tuberculosis ; Tuberculosis, Central Nervous System - metabolism ; Tuberculosis, Central Nervous System - microbiology ; Tuberculosis, Central Nervous System - pathology ; Up-Regulation ; Vascular endothelial growth factor ; Vascular Endothelial Growth Factor A - metabolism ; VEGF</subject><ispartof>Tuberculosis (Edinburgh, Scotland), 2018-09, Vol.112, p.45-51</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Science Ltd. Sep 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-d7fb3aaa511d82eaf9f43fee1d72c5735d4fa9d980a91f1e060caeb17aba61e3</citedby><cites>FETCH-LOGICAL-c384t-d7fb3aaa511d82eaf9f43fee1d72c5735d4fa9d980a91f1e060caeb17aba61e3</cites><orcidid>0000-0002-0258-7410</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1472979218302853$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30205968$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Othman, Fatihah N.</creatorcontrib><creatorcontrib>Muthuraju, Sangu</creatorcontrib><creatorcontrib>Noor, Siti Suraiya M.</creatorcontrib><creatorcontrib>Abdullah, Sarimah</creatorcontrib><creatorcontrib>Mohd Yusoff, Abdul Aziz</creatorcontrib><creatorcontrib>Tharakan, John</creatorcontrib><creatorcontrib>Bhaskar, Shalini</creatorcontrib><creatorcontrib>Mahmood, Mohd Shah</creatorcontrib><creatorcontrib>Kassim, Fauziah</creatorcontrib><creatorcontrib>Rafia, Hanip</creatorcontrib><creatorcontrib>Mohd Haspani, Mohd Saffari</creatorcontrib><creatorcontrib>Alias, Azmi</creatorcontrib><creatorcontrib>Pando, Rogelio H.</creatorcontrib><creatorcontrib>Abdullah, Jafri M.</creatorcontrib><creatorcontrib>Jaafar, Hasnan</creatorcontrib><title>Human tuberculosis brain promotes neuronal apoptosis but not in astrocytes with high expression of vascular endothelial growth factor</title><title>Tuberculosis (Edinburgh, Scotland)</title><addtitle>Tuberculosis (Edinb)</addtitle><description>The present study aimed to investigate the involvement of the angiogenic marker vascular endothelia growth factor (VEGF) and apoptotic markers of Bcl-2 and Bax in the neurons and astrocytes in the brain infected by Mycobacterium tuberculosis. The immunohistochemistry staining was performed to analyze the expression of the VEGF, Bcl-2 and Bax in the astrocytes and neurons. The expression of VEGF was high in neurons and astrocytes in both the infected brain and control tissues with no difference of angiogenic activity (p = 0.40). Higher Bcl-2 expression was seen in astrocytes of infected brain tissues compared to the control tissues (p = 0.004) promoted a higher anti-apoptotic activity in astrocytes. The neurons expressed strong Bax expression in the infected brain tissues compared to the control tissues (p < 0.001), which indicated more apoptosis in neurons. Thus, neuronal death and survival of infected astrocytes together with high expression of VEGF might be associated with formation of brain tuberculosis. In conclusion, neurons could be more vulnerable than astrocytes in human tuberculosis brain with high expression of VEGF.</description><subject>Angiogenesis</subject><subject>Apoptosis</subject><subject>Astrocyte</subject><subject>Astrocytes</subject><subject>Astrocytes - metabolism</subject><subject>Astrocytes - microbiology</subject><subject>Astrocytes - pathology</subject><subject>Bacteria</subject><subject>Bcl-2 protein</subject><subject>bcl-2-Associated X Protein - metabolism</subject><subject>Bcl2 and Bax</subject><subject>Brain</subject><subject>Case-Control Studies</subject><subject>Growth factors</subject><subject>Human brain tuberculosis</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Mycobacterium tuberculosis - pathogenicity</subject><subject>Neurons</subject><subject>Neurons - metabolism</subject><subject>Neurons - microbiology</subject><subject>Neurons - pathology</subject><subject>Proto-Oncogene Proteins c-bcl-2 - metabolism</subject><subject>Signal Transduction</subject><subject>Tissues</subject><subject>Tuberculosis</subject><subject>Tuberculosis, Central Nervous System - metabolism</subject><subject>Tuberculosis, Central Nervous System - microbiology</subject><subject>Tuberculosis, Central Nervous System - pathology</subject><subject>Up-Regulation</subject><subject>Vascular endothelial growth factor</subject><subject>Vascular Endothelial Growth Factor A - metabolism</subject><subject>VEGF</subject><issn>1472-9792</issn><issn>1873-281X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kbFuFDEURS0EIiHJD1AgSzQ0M7E9u2tbokEREKRIaVLQWW_s56xXM-PB9iTkA_hvPNpAQUHlV5x7JN9LyFvOWs747vLQlqXHVjCuWiZbxuQLcsqV7Bqh-PeX9d5I0WipxQl5k_OB1RBT7DU56ZhgW71Tp-TX9TLCRFdRsssQc8i0TxAmOqc4xoKZTrikOMFAYY5zORJLoVMstGKQS4r2aQUfQ9nTfbjfU_w5J8w5xIlGTx8gVzUkipOLZY9DqLL7FB8r7sGWmM7JKw9Dxovn94zcffl8d3Xd3Nx-_Xb16aaxndqUxknfdwCw5dwpgeC133QekTsp7FZ2W7fxoJ1WDDT3HNmOWcCeS-hhx7E7Ix-O2vq3HwvmYsaQLQ4DTBiXbARnQnPFVVfR9_-gh7ik2sJKca07qbWolDhSNsWcE3ozpzBCejKcmXUjczBrtWbdyDBp6kY19O5ZvfQjur-RP6NU4OMRwFrFQ8Bksg04WXQhoS3GxfA__2835KdJ</recordid><startdate>201809</startdate><enddate>201809</enddate><creator>Othman, Fatihah N.</creator><creator>Muthuraju, Sangu</creator><creator>Noor, Siti Suraiya M.</creator><creator>Abdullah, Sarimah</creator><creator>Mohd Yusoff, Abdul Aziz</creator><creator>Tharakan, John</creator><creator>Bhaskar, Shalini</creator><creator>Mahmood, Mohd Shah</creator><creator>Kassim, Fauziah</creator><creator>Rafia, Hanip</creator><creator>Mohd Haspani, Mohd Saffari</creator><creator>Alias, Azmi</creator><creator>Pando, Rogelio H.</creator><creator>Abdullah, Jafri M.</creator><creator>Jaafar, Hasnan</creator><general>Elsevier Ltd</general><general>Elsevier Science Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0258-7410</orcidid></search><sort><creationdate>201809</creationdate><title>Human tuberculosis brain promotes neuronal apoptosis but not in astrocytes with high expression of vascular endothelial growth factor</title><author>Othman, Fatihah N. ; Muthuraju, Sangu ; Noor, Siti Suraiya M. ; Abdullah, Sarimah ; Mohd Yusoff, Abdul Aziz ; Tharakan, John ; Bhaskar, Shalini ; Mahmood, Mohd Shah ; Kassim, Fauziah ; Rafia, Hanip ; Mohd Haspani, Mohd Saffari ; Alias, Azmi ; Pando, Rogelio H. ; Abdullah, Jafri M. ; Jaafar, Hasnan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-d7fb3aaa511d82eaf9f43fee1d72c5735d4fa9d980a91f1e060caeb17aba61e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Angiogenesis</topic><topic>Apoptosis</topic><topic>Astrocyte</topic><topic>Astrocytes</topic><topic>Astrocytes - metabolism</topic><topic>Astrocytes - microbiology</topic><topic>Astrocytes - pathology</topic><topic>Bacteria</topic><topic>Bcl-2 protein</topic><topic>bcl-2-Associated X Protein - metabolism</topic><topic>Bcl2 and Bax</topic><topic>Brain</topic><topic>Case-Control Studies</topic><topic>Growth factors</topic><topic>Human brain tuberculosis</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Mycobacterium tuberculosis - pathogenicity</topic><topic>Neurons</topic><topic>Neurons - metabolism</topic><topic>Neurons - microbiology</topic><topic>Neurons - pathology</topic><topic>Proto-Oncogene Proteins c-bcl-2 - metabolism</topic><topic>Signal Transduction</topic><topic>Tissues</topic><topic>Tuberculosis</topic><topic>Tuberculosis, Central Nervous System - metabolism</topic><topic>Tuberculosis, Central Nervous System - microbiology</topic><topic>Tuberculosis, Central Nervous System - pathology</topic><topic>Up-Regulation</topic><topic>Vascular endothelial growth factor</topic><topic>Vascular Endothelial Growth Factor A - metabolism</topic><topic>VEGF</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Othman, Fatihah N.</creatorcontrib><creatorcontrib>Muthuraju, Sangu</creatorcontrib><creatorcontrib>Noor, Siti Suraiya M.</creatorcontrib><creatorcontrib>Abdullah, Sarimah</creatorcontrib><creatorcontrib>Mohd Yusoff, Abdul Aziz</creatorcontrib><creatorcontrib>Tharakan, John</creatorcontrib><creatorcontrib>Bhaskar, Shalini</creatorcontrib><creatorcontrib>Mahmood, Mohd Shah</creatorcontrib><creatorcontrib>Kassim, Fauziah</creatorcontrib><creatorcontrib>Rafia, Hanip</creatorcontrib><creatorcontrib>Mohd Haspani, Mohd Saffari</creatorcontrib><creatorcontrib>Alias, Azmi</creatorcontrib><creatorcontrib>Pando, Rogelio H.</creatorcontrib><creatorcontrib>Abdullah, Jafri M.</creatorcontrib><creatorcontrib>Jaafar, Hasnan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Tuberculosis (Edinburgh, Scotland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Othman, Fatihah N.</au><au>Muthuraju, Sangu</au><au>Noor, Siti Suraiya M.</au><au>Abdullah, Sarimah</au><au>Mohd Yusoff, Abdul Aziz</au><au>Tharakan, John</au><au>Bhaskar, Shalini</au><au>Mahmood, Mohd Shah</au><au>Kassim, Fauziah</au><au>Rafia, Hanip</au><au>Mohd Haspani, Mohd Saffari</au><au>Alias, Azmi</au><au>Pando, Rogelio H.</au><au>Abdullah, Jafri M.</au><au>Jaafar, Hasnan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human tuberculosis brain promotes neuronal apoptosis but not in astrocytes with high expression of vascular endothelial growth factor</atitle><jtitle>Tuberculosis (Edinburgh, Scotland)</jtitle><addtitle>Tuberculosis (Edinb)</addtitle><date>2018-09</date><risdate>2018</risdate><volume>112</volume><spage>45</spage><epage>51</epage><pages>45-51</pages><issn>1472-9792</issn><eissn>1873-281X</eissn><abstract>The present study aimed to investigate the involvement of the angiogenic marker vascular endothelia growth factor (VEGF) and apoptotic markers of Bcl-2 and Bax in the neurons and astrocytes in the brain infected by Mycobacterium tuberculosis. The immunohistochemistry staining was performed to analyze the expression of the VEGF, Bcl-2 and Bax in the astrocytes and neurons. The expression of VEGF was high in neurons and astrocytes in both the infected brain and control tissues with no difference of angiogenic activity (p = 0.40). Higher Bcl-2 expression was seen in astrocytes of infected brain tissues compared to the control tissues (p = 0.004) promoted a higher anti-apoptotic activity in astrocytes. The neurons expressed strong Bax expression in the infected brain tissues compared to the control tissues (p < 0.001), which indicated more apoptosis in neurons. Thus, neuronal death and survival of infected astrocytes together with high expression of VEGF might be associated with formation of brain tuberculosis. 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| subjects | Angiogenesis Apoptosis Astrocyte Astrocytes Astrocytes - metabolism Astrocytes - microbiology Astrocytes - pathology Bacteria Bcl-2 protein bcl-2-Associated X Protein - metabolism Bcl2 and Bax Brain Case-Control Studies Growth factors Human brain tuberculosis Humans Immunohistochemistry Mycobacterium tuberculosis - pathogenicity Neurons Neurons - metabolism Neurons - microbiology Neurons - pathology Proto-Oncogene Proteins c-bcl-2 - metabolism Signal Transduction Tissues Tuberculosis Tuberculosis, Central Nervous System - metabolism Tuberculosis, Central Nervous System - microbiology Tuberculosis, Central Nervous System - pathology Up-Regulation Vascular endothelial growth factor Vascular Endothelial Growth Factor A - metabolism VEGF |
| title | Human tuberculosis brain promotes neuronal apoptosis but not in astrocytes with high expression of vascular endothelial growth factor |
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