Prognostic Impact of Immune-Related Gene Expression in Preoperative Peripheral Blood from Gastric Cancer Patients
Background Anti-PD-1 therapy has shown a promising clinical outcome in gastric cancer (GC). We evaluated the clinical significance of systemic immune-related gene expression in GC patients who underwent surgery. Methods The correlation between the preoperative PD - 1 , PD - L1, and CD8 mRNA levels i...
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| Veröffentlicht in: | Annals of surgical oncology 2018-11, Vol.25 (12), p.3755-3763 |
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| creator | Ito, Shuhei Fukagawa, Takeo Noda, Miwa Hu, Qingjiang Nambara, Sho Shimizu, Dai Kuroda, Yosuke Eguchi, Hidetoshi Masuda, Takaaki Sato, Tetsuya Katai, Hitoshi Sasako, Mitsuru Mimori, Koshi |
| description | Background
Anti-PD-1 therapy has shown a promising clinical outcome in gastric cancer (GC). We evaluated the clinical significance of systemic immune-related gene expression in GC patients who underwent surgery.
Methods
The correlation between the preoperative
PD
-
1
,
PD
-
L1,
and
CD8
mRNA levels in peripheral blood (PB) and clinicopathological factors, including survival, in 372 GC patients was evaluated using quantitative RT-PCR. PD-1- and PD-L1-expressing cells were identified by flow cytometric analysis.
Results
The
PD
-
1
,
PD
-
L1,
and
CD8
mRNA levels in GC patients were significantly higher than those in normal controls, respectively (all
P
|
| doi_str_mv | 10.1245/s10434-018-6739-4 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2102320272</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2102320272</sourcerecordid><originalsourceid>FETCH-LOGICAL-c410t-d1ae000a6d542364bad688ce61754c2eed1b4c934228dd880d1577c32c38d1403</originalsourceid><addsrcrecordid>eNp9kMtOwzAQRS0E4v0BbJCXbALjRxJ3CRWUSkhUCNaWa09LUGIHO0Hw97gqsGQ1M_aZK80h5IzBJeOyvEoMpJAFMFVUtZgUcoccsjK_yEqx3dxDpYoJr8oDcpTSGwCrBZT75EAAByGhPiTvixjWPqShsXTe9cYONKxy140eiydszYCOztAjvf3sI6bUBE8bTxcRQ4_RDM0H0gXGpn_NU0tv2hAcXcXQ0ZlJQ8yxU-MtRrrILPohnZC9lWkTnv7UY_Jyd_s8vS8eHmfz6fVDYSWDoXDMIACYypWSi0oujauUslixupSWIzq2lHYiJOfKOaXAsbKureBWKMckiGNysc3tY3gfMQ26a5LFtjUew5g0Z8AFB17zjLItamNIKeJK97HpTPzSDPTGtN6a1tm03pjWMu-c_8SPyw7d38av2gzwLZDyl19j1G9hjD6f_E_qNyWeiV4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2102320272</pqid></control><display><type>article</type><title>Prognostic Impact of Immune-Related Gene Expression in Preoperative Peripheral Blood from Gastric Cancer Patients</title><source>MEDLINE</source><source>SpringerLink (Online service)</source><creator>Ito, Shuhei ; Fukagawa, Takeo ; Noda, Miwa ; Hu, Qingjiang ; Nambara, Sho ; Shimizu, Dai ; Kuroda, Yosuke ; Eguchi, Hidetoshi ; Masuda, Takaaki ; Sato, Tetsuya ; Katai, Hitoshi ; Sasako, Mitsuru ; Mimori, Koshi</creator><creatorcontrib>Ito, Shuhei ; Fukagawa, Takeo ; Noda, Miwa ; Hu, Qingjiang ; Nambara, Sho ; Shimizu, Dai ; Kuroda, Yosuke ; Eguchi, Hidetoshi ; Masuda, Takaaki ; Sato, Tetsuya ; Katai, Hitoshi ; Sasako, Mitsuru ; Mimori, Koshi</creatorcontrib><description><![CDATA[Background
Anti-PD-1 therapy has shown a promising clinical outcome in gastric cancer (GC). We evaluated the clinical significance of systemic immune-related gene expression in GC patients who underwent surgery.
Methods
The correlation between the preoperative
PD
-
1
,
PD
-
L1,
and
CD8
mRNA levels in peripheral blood (PB) and clinicopathological factors, including survival, in 372 GC patients was evaluated using quantitative RT-PCR. PD-1- and PD-L1-expressing cells were identified by flow cytometric analysis.
Results
The
PD
-
1
,
PD
-
L1,
and
CD8
mRNA levels in GC patients were significantly higher than those in normal controls, respectively (all
P
< 0.0001). The levels of each gene were positively correlated with those of the other two genes (all
P
< 0.0001). GC patients with low
PD
-
1,
high
PD
-
L1,
and low
CD8
mRNA levels had significantly poorer overall survival (OS) than those with high
PD
-
1
, low
PD
-
L1,
and high
CD8
mRNA levels, respectively (
P
< 0.01,
P
< 0.05, and
P
< 0.05, respectively). Multivariate analysis showed that low
PD
-
1
and high
PD
-
L1
mRNA levels were independent poor prognostic factors for OS (
PD
-
1
: HR 2.38, 95% CI 1.27–4.78,
P
< 0.01;
PD
-
L1
: HR 1.81, 95% CI 1.15–2.78,
P
< 0.05). PD-1 and PD-L1 expression occurred on T cells (> 90%) and T cells or monocytes (> 70%), respectively.
Conclusions
The
PD
-
1
,
PD
-
L1
, and
CD8
mRNA levels in preoperative PB reflected the anti-tumour immune response, and the low
PD
-
1
and high
PD
-
L1
mRNA levels in PB were independent poor prognostic markers in GC patients who underwent surgery.]]></description><identifier>ISSN: 1068-9265</identifier><identifier>EISSN: 1534-4681</identifier><identifier>DOI: 10.1245/s10434-018-6739-4</identifier><identifier>PMID: 30203407</identifier><language>eng</language><publisher>Cham: Springer International Publishing</publisher><subject>B7-H1 Antigen - genetics ; Biomarkers, Tumor - genetics ; Case-Control Studies ; CD8 Antigens - genetics ; Follow-Up Studies ; Humans ; Lymphatic Metastasis ; Medicine ; Medicine & Public Health ; Neoplasm Invasiveness ; Oncology ; Prognosis ; Programmed Cell Death 1 Receptor - genetics ; Stomach Neoplasms - genetics ; Stomach Neoplasms - pathology ; Stomach Neoplasms - surgery ; Surgery ; Surgical Oncology ; Survival Rate ; Translational Research and Biomarkers</subject><ispartof>Annals of surgical oncology, 2018-11, Vol.25 (12), p.3755-3763</ispartof><rights>Society of Surgical Oncology 2018</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c410t-d1ae000a6d542364bad688ce61754c2eed1b4c934228dd880d1577c32c38d1403</citedby><cites>FETCH-LOGICAL-c410t-d1ae000a6d542364bad688ce61754c2eed1b4c934228dd880d1577c32c38d1403</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1245/s10434-018-6739-4$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1245/s10434-018-6739-4$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30203407$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ito, Shuhei</creatorcontrib><creatorcontrib>Fukagawa, Takeo</creatorcontrib><creatorcontrib>Noda, Miwa</creatorcontrib><creatorcontrib>Hu, Qingjiang</creatorcontrib><creatorcontrib>Nambara, Sho</creatorcontrib><creatorcontrib>Shimizu, Dai</creatorcontrib><creatorcontrib>Kuroda, Yosuke</creatorcontrib><creatorcontrib>Eguchi, Hidetoshi</creatorcontrib><creatorcontrib>Masuda, Takaaki</creatorcontrib><creatorcontrib>Sato, Tetsuya</creatorcontrib><creatorcontrib>Katai, Hitoshi</creatorcontrib><creatorcontrib>Sasako, Mitsuru</creatorcontrib><creatorcontrib>Mimori, Koshi</creatorcontrib><title>Prognostic Impact of Immune-Related Gene Expression in Preoperative Peripheral Blood from Gastric Cancer Patients</title><title>Annals of surgical oncology</title><addtitle>Ann Surg Oncol</addtitle><addtitle>Ann Surg Oncol</addtitle><description><![CDATA[Background
Anti-PD-1 therapy has shown a promising clinical outcome in gastric cancer (GC). We evaluated the clinical significance of systemic immune-related gene expression in GC patients who underwent surgery.
Methods
The correlation between the preoperative
PD
-
1
,
PD
-
L1,
and
CD8
mRNA levels in peripheral blood (PB) and clinicopathological factors, including survival, in 372 GC patients was evaluated using quantitative RT-PCR. PD-1- and PD-L1-expressing cells were identified by flow cytometric analysis.
Results
The
PD
-
1
,
PD
-
L1,
and
CD8
mRNA levels in GC patients were significantly higher than those in normal controls, respectively (all
P
< 0.0001). The levels of each gene were positively correlated with those of the other two genes (all
P
< 0.0001). GC patients with low
PD
-
1,
high
PD
-
L1,
and low
CD8
mRNA levels had significantly poorer overall survival (OS) than those with high
PD
-
1
, low
PD
-
L1,
and high
CD8
mRNA levels, respectively (
P
< 0.01,
P
< 0.05, and
P
< 0.05, respectively). Multivariate analysis showed that low
PD
-
1
and high
PD
-
L1
mRNA levels were independent poor prognostic factors for OS (
PD
-
1
: HR 2.38, 95% CI 1.27–4.78,
P
< 0.01;
PD
-
L1
: HR 1.81, 95% CI 1.15–2.78,
P
< 0.05). PD-1 and PD-L1 expression occurred on T cells (> 90%) and T cells or monocytes (> 70%), respectively.
Conclusions
The
PD
-
1
,
PD
-
L1
, and
CD8
mRNA levels in preoperative PB reflected the anti-tumour immune response, and the low
PD
-
1
and high
PD
-
L1
mRNA levels in PB were independent poor prognostic markers in GC patients who underwent surgery.]]></description><subject>B7-H1 Antigen - genetics</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Case-Control Studies</subject><subject>CD8 Antigens - genetics</subject><subject>Follow-Up Studies</subject><subject>Humans</subject><subject>Lymphatic Metastasis</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>Neoplasm Invasiveness</subject><subject>Oncology</subject><subject>Prognosis</subject><subject>Programmed Cell Death 1 Receptor - genetics</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - pathology</subject><subject>Stomach Neoplasms - surgery</subject><subject>Surgery</subject><subject>Surgical Oncology</subject><subject>Survival Rate</subject><subject>Translational Research and Biomarkers</subject><issn>1068-9265</issn><issn>1534-4681</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0E4v0BbJCXbALjRxJ3CRWUSkhUCNaWa09LUGIHO0Hw97gqsGQ1M_aZK80h5IzBJeOyvEoMpJAFMFVUtZgUcoccsjK_yEqx3dxDpYoJr8oDcpTSGwCrBZT75EAAByGhPiTvixjWPqShsXTe9cYONKxy140eiydszYCOztAjvf3sI6bUBE8bTxcRQ4_RDM0H0gXGpn_NU0tv2hAcXcXQ0ZlJQ8yxU-MtRrrILPohnZC9lWkTnv7UY_Jyd_s8vS8eHmfz6fVDYSWDoXDMIACYypWSi0oujauUslixupSWIzq2lHYiJOfKOaXAsbKureBWKMckiGNysc3tY3gfMQ26a5LFtjUew5g0Z8AFB17zjLItamNIKeJK97HpTPzSDPTGtN6a1tm03pjWMu-c_8SPyw7d38av2gzwLZDyl19j1G9hjD6f_E_qNyWeiV4</recordid><startdate>20181101</startdate><enddate>20181101</enddate><creator>Ito, Shuhei</creator><creator>Fukagawa, Takeo</creator><creator>Noda, Miwa</creator><creator>Hu, Qingjiang</creator><creator>Nambara, Sho</creator><creator>Shimizu, Dai</creator><creator>Kuroda, Yosuke</creator><creator>Eguchi, Hidetoshi</creator><creator>Masuda, Takaaki</creator><creator>Sato, Tetsuya</creator><creator>Katai, Hitoshi</creator><creator>Sasako, Mitsuru</creator><creator>Mimori, Koshi</creator><general>Springer International Publishing</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20181101</creationdate><title>Prognostic Impact of Immune-Related Gene Expression in Preoperative Peripheral Blood from Gastric Cancer Patients</title><author>Ito, Shuhei ; Fukagawa, Takeo ; Noda, Miwa ; Hu, Qingjiang ; Nambara, Sho ; Shimizu, Dai ; Kuroda, Yosuke ; Eguchi, Hidetoshi ; Masuda, Takaaki ; Sato, Tetsuya ; Katai, Hitoshi ; Sasako, Mitsuru ; Mimori, Koshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c410t-d1ae000a6d542364bad688ce61754c2eed1b4c934228dd880d1577c32c38d1403</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>B7-H1 Antigen - genetics</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Case-Control Studies</topic><topic>CD8 Antigens - genetics</topic><topic>Follow-Up Studies</topic><topic>Humans</topic><topic>Lymphatic Metastasis</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>Neoplasm Invasiveness</topic><topic>Oncology</topic><topic>Prognosis</topic><topic>Programmed Cell Death 1 Receptor - genetics</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - pathology</topic><topic>Stomach Neoplasms - surgery</topic><topic>Surgery</topic><topic>Surgical Oncology</topic><topic>Survival Rate</topic><topic>Translational Research and Biomarkers</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ito, Shuhei</creatorcontrib><creatorcontrib>Fukagawa, Takeo</creatorcontrib><creatorcontrib>Noda, Miwa</creatorcontrib><creatorcontrib>Hu, Qingjiang</creatorcontrib><creatorcontrib>Nambara, Sho</creatorcontrib><creatorcontrib>Shimizu, Dai</creatorcontrib><creatorcontrib>Kuroda, Yosuke</creatorcontrib><creatorcontrib>Eguchi, Hidetoshi</creatorcontrib><creatorcontrib>Masuda, Takaaki</creatorcontrib><creatorcontrib>Sato, Tetsuya</creatorcontrib><creatorcontrib>Katai, Hitoshi</creatorcontrib><creatorcontrib>Sasako, Mitsuru</creatorcontrib><creatorcontrib>Mimori, Koshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Annals of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ito, Shuhei</au><au>Fukagawa, Takeo</au><au>Noda, Miwa</au><au>Hu, Qingjiang</au><au>Nambara, Sho</au><au>Shimizu, Dai</au><au>Kuroda, Yosuke</au><au>Eguchi, Hidetoshi</au><au>Masuda, Takaaki</au><au>Sato, Tetsuya</au><au>Katai, Hitoshi</au><au>Sasako, Mitsuru</au><au>Mimori, Koshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prognostic Impact of Immune-Related Gene Expression in Preoperative Peripheral Blood from Gastric Cancer Patients</atitle><jtitle>Annals of surgical oncology</jtitle><stitle>Ann Surg Oncol</stitle><addtitle>Ann Surg Oncol</addtitle><date>2018-11-01</date><risdate>2018</risdate><volume>25</volume><issue>12</issue><spage>3755</spage><epage>3763</epage><pages>3755-3763</pages><issn>1068-9265</issn><eissn>1534-4681</eissn><abstract><![CDATA[Background
Anti-PD-1 therapy has shown a promising clinical outcome in gastric cancer (GC). We evaluated the clinical significance of systemic immune-related gene expression in GC patients who underwent surgery.
Methods
The correlation between the preoperative
PD
-
1
,
PD
-
L1,
and
CD8
mRNA levels in peripheral blood (PB) and clinicopathological factors, including survival, in 372 GC patients was evaluated using quantitative RT-PCR. PD-1- and PD-L1-expressing cells were identified by flow cytometric analysis.
Results
The
PD
-
1
,
PD
-
L1,
and
CD8
mRNA levels in GC patients were significantly higher than those in normal controls, respectively (all
P
< 0.0001). The levels of each gene were positively correlated with those of the other two genes (all
P
< 0.0001). GC patients with low
PD
-
1,
high
PD
-
L1,
and low
CD8
mRNA levels had significantly poorer overall survival (OS) than those with high
PD
-
1
, low
PD
-
L1,
and high
CD8
mRNA levels, respectively (
P
< 0.01,
P
< 0.05, and
P
< 0.05, respectively). Multivariate analysis showed that low
PD
-
1
and high
PD
-
L1
mRNA levels were independent poor prognostic factors for OS (
PD
-
1
: HR 2.38, 95% CI 1.27–4.78,
P
< 0.01;
PD
-
L1
: HR 1.81, 95% CI 1.15–2.78,
P
< 0.05). PD-1 and PD-L1 expression occurred on T cells (> 90%) and T cells or monocytes (> 70%), respectively.
Conclusions
The
PD
-
1
,
PD
-
L1
, and
CD8
mRNA levels in preoperative PB reflected the anti-tumour immune response, and the low
PD
-
1
and high
PD
-
L1
mRNA levels in PB were independent poor prognostic markers in GC patients who underwent surgery.]]></abstract><cop>Cham</cop><pub>Springer International Publishing</pub><pmid>30203407</pmid><doi>10.1245/s10434-018-6739-4</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1068-9265 |
| ispartof | Annals of surgical oncology, 2018-11, Vol.25 (12), p.3755-3763 |
| issn | 1068-9265 1534-4681 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2102320272 |
| source | MEDLINE; SpringerLink (Online service) |
| subjects | B7-H1 Antigen - genetics Biomarkers, Tumor - genetics Case-Control Studies CD8 Antigens - genetics Follow-Up Studies Humans Lymphatic Metastasis Medicine Medicine & Public Health Neoplasm Invasiveness Oncology Prognosis Programmed Cell Death 1 Receptor - genetics Stomach Neoplasms - genetics Stomach Neoplasms - pathology Stomach Neoplasms - surgery Surgery Surgical Oncology Survival Rate Translational Research and Biomarkers |
| title | Prognostic Impact of Immune-Related Gene Expression in Preoperative Peripheral Blood from Gastric Cancer Patients |
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