Uterine inflammation and fertility of beef cows subjected to timed AI at different days postpartum
The aim of this study was to evaluate the uterine health and fertility of postpartum beef cows subjected to timed AI (TAI) protocols at different days from calving. Lactating Nelore cows (Bos indicus; n = 244) were used in this study. The TAI protocols were initiated between 20 and 60 days postpartu...
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| Veröffentlicht in: | Animal reproduction science 2018-10, Vol.197, p.268-277 |
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| creator | Machado Pfeifer, Luiz Francisco de Souza Andrade, Jéssica Moreira, Elizângela Mírian Reis da Silva, Renata Araújo Neves, Paulo Marcos Moreira da Silva, George Lemos, Izabela Cristina Schneider, Augusto |
| description | The aim of this study was to evaluate the uterine health and fertility of postpartum beef cows subjected to timed AI (TAI) protocols at different days from calving. Lactating Nelore cows (Bos indicus; n = 244) were used in this study. The TAI protocols were initiated between 20 and 60 days postpartum (DPP). Cows were subjected to an estradiol-progesterone based TAI protocol. The cows were divided into three groups according to the days postpartum at the time the hormonal treatment was initiated: 1) Early (n = 64), cows between 20 and 30 DPP; 2) Middle (n = 115), cows between 31 and 45 DPP; and 3) Late (n = 65), cows between 46 and 60 DPP. At Day 0 of the protocol, endometrial cytobrush samples were collected. Slides for polymorphonuclear (PMN) cell counting were prepared and cytobrush was used for RNA extraction and analysis of relative abundance of il1, il6, il8, and tnf mRNA. Cows from the Early group had less (P |
| doi_str_mv | 10.1016/j.anireprosci.2018.08.039 |
| format | Article |
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Lactating Nelore cows (Bos indicus; n = 244) were used in this study. The TAI protocols were initiated between 20 and 60 days postpartum (DPP). Cows were subjected to an estradiol-progesterone based TAI protocol. The cows were divided into three groups according to the days postpartum at the time the hormonal treatment was initiated: 1) Early (n = 64), cows between 20 and 30 DPP; 2) Middle (n = 115), cows between 31 and 45 DPP; and 3) Late (n = 65), cows between 46 and 60 DPP. At Day 0 of the protocol, endometrial cytobrush samples were collected. Slides for polymorphonuclear (PMN) cell counting were prepared and cytobrush was used for RNA extraction and analysis of relative abundance of il1, il6, il8, and tnf mRNA. Cows from the Early group had less (P < 0.05) pregnancies per AI (P/AI) than cows from Middle and Late groups; 29.7% (19/64), 45.2% (52/115), and 52.31% (34/65), respectively. Accordingly, the Early group had a greater (P < 0.05) proportion of PMN cells in the uterus than Middle and Late groups; 9.0%, 3.8%, and 2.2%, respectively. Relative abundances of il1 and il8 mRNA were greater (P < 0.05) in the Early group than Middle and Late groups. These results indicate that beef cows subjected to TAI protocols early postpartum (< 30 DPP) are less likely to become pregnant, which is associated with increased inflammation and relative abundance of mRNA for the proinflammatory cytokines il1 and il8.</description><identifier>ISSN: 0378-4320</identifier><identifier>EISSN: 1873-2232</identifier><identifier>DOI: 10.1016/j.anireprosci.2018.08.039</identifier><identifier>PMID: 30195943</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Calving ; Cattle ; Cattle Diseases - physiopathology ; Cytobrush ; Cytokines ; Estrus Synchronization ; Female ; Fertility - physiology ; Inflammation - physiopathology ; Inflammation - veterinary ; Insemination, Artificial - methods ; Insemination, Artificial - veterinary ; Lactation ; PMN cell ; Postpartum Period ; Pregnancy ; Progesterone ; Uterine Diseases - physiopathology ; Uterine Diseases - veterinary</subject><ispartof>Animal reproduction science, 2018-10, Vol.197, p.268-277</ispartof><rights>2018</rights><rights>Copyright © 2018. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-73db09f7230ce58112ca6910c5924daf15b37c6f7be4ad084277a1eaef0da30d3</citedby><cites>FETCH-LOGICAL-c428t-73db09f7230ce58112ca6910c5924daf15b37c6f7be4ad084277a1eaef0da30d3</cites><orcidid>0000-0003-2069-951X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378432018306201$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30195943$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Machado Pfeifer, Luiz Francisco</creatorcontrib><creatorcontrib>de Souza Andrade, Jéssica</creatorcontrib><creatorcontrib>Moreira, Elizângela Mírian</creatorcontrib><creatorcontrib>Reis da Silva, Renata</creatorcontrib><creatorcontrib>Araújo Neves, Paulo Marcos</creatorcontrib><creatorcontrib>Moreira da Silva, George</creatorcontrib><creatorcontrib>Lemos, Izabela Cristina</creatorcontrib><creatorcontrib>Schneider, Augusto</creatorcontrib><title>Uterine inflammation and fertility of beef cows subjected to timed AI at different days postpartum</title><title>Animal reproduction science</title><addtitle>Anim Reprod Sci</addtitle><description>The aim of this study was to evaluate the uterine health and fertility of postpartum beef cows subjected to timed AI (TAI) protocols at different days from calving. Lactating Nelore cows (Bos indicus; n = 244) were used in this study. The TAI protocols were initiated between 20 and 60 days postpartum (DPP). Cows were subjected to an estradiol-progesterone based TAI protocol. The cows were divided into three groups according to the days postpartum at the time the hormonal treatment was initiated: 1) Early (n = 64), cows between 20 and 30 DPP; 2) Middle (n = 115), cows between 31 and 45 DPP; and 3) Late (n = 65), cows between 46 and 60 DPP. At Day 0 of the protocol, endometrial cytobrush samples were collected. Slides for polymorphonuclear (PMN) cell counting were prepared and cytobrush was used for RNA extraction and analysis of relative abundance of il1, il6, il8, and tnf mRNA. Cows from the Early group had less (P < 0.05) pregnancies per AI (P/AI) than cows from Middle and Late groups; 29.7% (19/64), 45.2% (52/115), and 52.31% (34/65), respectively. Accordingly, the Early group had a greater (P < 0.05) proportion of PMN cells in the uterus than Middle and Late groups; 9.0%, 3.8%, and 2.2%, respectively. Relative abundances of il1 and il8 mRNA were greater (P < 0.05) in the Early group than Middle and Late groups. These results indicate that beef cows subjected to TAI protocols early postpartum (< 30 DPP) are less likely to become pregnant, which is associated with increased inflammation and relative abundance of mRNA for the proinflammatory cytokines il1 and il8.</description><subject>Animals</subject><subject>Calving</subject><subject>Cattle</subject><subject>Cattle Diseases - physiopathology</subject><subject>Cytobrush</subject><subject>Cytokines</subject><subject>Estrus Synchronization</subject><subject>Female</subject><subject>Fertility - physiology</subject><subject>Inflammation - physiopathology</subject><subject>Inflammation - veterinary</subject><subject>Insemination, Artificial - methods</subject><subject>Insemination, Artificial - veterinary</subject><subject>Lactation</subject><subject>PMN cell</subject><subject>Postpartum Period</subject><subject>Pregnancy</subject><subject>Progesterone</subject><subject>Uterine Diseases - physiopathology</subject><subject>Uterine Diseases - veterinary</subject><issn>0378-4320</issn><issn>1873-2232</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE9rGzEQxUVJiB0nX6Got1zWHUnr1eoYTNMGDLnUZ6GVRiCzf1xJ2-JvHxm7ocfCwMzhvTe8HyFfGKwZsObrYW3GEPEYp2TDmgNr11BGqE9kyVopKs4FvyFLELKtasFhQe5TOgCAbBp1RxYCmNqoWixJt88Yw4g0jL43w2BymEZqRkc9xhz6kE908rRD9NROfxJNc3dAm9HRPNEchnI8v1KTqQu-WHAslzklepxSPpqY5-GB3HrTJ3y87hXZv3z7uf1R7d6-v26fd5WteZsrKVwHyksuwOKmZYxb0ygGdqN47Yxnm05I23jZYW0ctDWX0jA06MEZAU6syNMlt3D5NWPKegjJYt-bEac5aV7YKdYIXhepukhtQZgien2MYTDxpBnoM2J90P8g1mfEGsoIVbyfr2_mrrT_cP5lWgTbiwBL2d8Boy4ROFp0JdBm7abwH2_eAd8ClLw</recordid><startdate>201810</startdate><enddate>201810</enddate><creator>Machado Pfeifer, Luiz Francisco</creator><creator>de Souza Andrade, Jéssica</creator><creator>Moreira, Elizângela Mírian</creator><creator>Reis da Silva, Renata</creator><creator>Araújo Neves, Paulo Marcos</creator><creator>Moreira da Silva, George</creator><creator>Lemos, Izabela Cristina</creator><creator>Schneider, Augusto</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2069-951X</orcidid></search><sort><creationdate>201810</creationdate><title>Uterine inflammation and fertility of beef cows subjected to timed AI at different days postpartum</title><author>Machado Pfeifer, Luiz Francisco ; de Souza Andrade, Jéssica ; Moreira, Elizângela Mírian ; Reis da Silva, Renata ; Araújo Neves, Paulo Marcos ; Moreira da Silva, George ; Lemos, Izabela Cristina ; Schneider, Augusto</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-73db09f7230ce58112ca6910c5924daf15b37c6f7be4ad084277a1eaef0da30d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Calving</topic><topic>Cattle</topic><topic>Cattle Diseases - physiopathology</topic><topic>Cytobrush</topic><topic>Cytokines</topic><topic>Estrus Synchronization</topic><topic>Female</topic><topic>Fertility - physiology</topic><topic>Inflammation - physiopathology</topic><topic>Inflammation - veterinary</topic><topic>Insemination, Artificial - methods</topic><topic>Insemination, Artificial - veterinary</topic><topic>Lactation</topic><topic>PMN cell</topic><topic>Postpartum Period</topic><topic>Pregnancy</topic><topic>Progesterone</topic><topic>Uterine Diseases - physiopathology</topic><topic>Uterine Diseases - veterinary</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Machado Pfeifer, Luiz Francisco</creatorcontrib><creatorcontrib>de Souza Andrade, Jéssica</creatorcontrib><creatorcontrib>Moreira, Elizângela Mírian</creatorcontrib><creatorcontrib>Reis da Silva, Renata</creatorcontrib><creatorcontrib>Araújo Neves, Paulo Marcos</creatorcontrib><creatorcontrib>Moreira da Silva, George</creatorcontrib><creatorcontrib>Lemos, Izabela Cristina</creatorcontrib><creatorcontrib>Schneider, Augusto</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Animal reproduction science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Machado Pfeifer, Luiz Francisco</au><au>de Souza Andrade, Jéssica</au><au>Moreira, Elizângela Mírian</au><au>Reis da Silva, Renata</au><au>Araújo Neves, Paulo Marcos</au><au>Moreira da Silva, George</au><au>Lemos, Izabela Cristina</au><au>Schneider, Augusto</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Uterine inflammation and fertility of beef cows subjected to timed AI at different days postpartum</atitle><jtitle>Animal reproduction science</jtitle><addtitle>Anim Reprod Sci</addtitle><date>2018-10</date><risdate>2018</risdate><volume>197</volume><spage>268</spage><epage>277</epage><pages>268-277</pages><issn>0378-4320</issn><eissn>1873-2232</eissn><abstract>The aim of this study was to evaluate the uterine health and fertility of postpartum beef cows subjected to timed AI (TAI) protocols at different days from calving. Lactating Nelore cows (Bos indicus; n = 244) were used in this study. The TAI protocols were initiated between 20 and 60 days postpartum (DPP). Cows were subjected to an estradiol-progesterone based TAI protocol. The cows were divided into three groups according to the days postpartum at the time the hormonal treatment was initiated: 1) Early (n = 64), cows between 20 and 30 DPP; 2) Middle (n = 115), cows between 31 and 45 DPP; and 3) Late (n = 65), cows between 46 and 60 DPP. At Day 0 of the protocol, endometrial cytobrush samples were collected. Slides for polymorphonuclear (PMN) cell counting were prepared and cytobrush was used for RNA extraction and analysis of relative abundance of il1, il6, il8, and tnf mRNA. Cows from the Early group had less (P < 0.05) pregnancies per AI (P/AI) than cows from Middle and Late groups; 29.7% (19/64), 45.2% (52/115), and 52.31% (34/65), respectively. Accordingly, the Early group had a greater (P < 0.05) proportion of PMN cells in the uterus than Middle and Late groups; 9.0%, 3.8%, and 2.2%, respectively. Relative abundances of il1 and il8 mRNA were greater (P < 0.05) in the Early group than Middle and Late groups. These results indicate that beef cows subjected to TAI protocols early postpartum (< 30 DPP) are less likely to become pregnant, which is associated with increased inflammation and relative abundance of mRNA for the proinflammatory cytokines il1 and il8.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30195943</pmid><doi>10.1016/j.anireprosci.2018.08.039</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0003-2069-951X</orcidid><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Calving Cattle Cattle Diseases - physiopathology Cytobrush Cytokines Estrus Synchronization Female Fertility - physiology Inflammation - physiopathology Inflammation - veterinary Insemination, Artificial - methods Insemination, Artificial - veterinary Lactation PMN cell Postpartum Period Pregnancy Progesterone Uterine Diseases - physiopathology Uterine Diseases - veterinary |
| title | Uterine inflammation and fertility of beef cows subjected to timed AI at different days postpartum |
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