Separation of cells in density gradients with temporary solidification of the gradient: Application to Escherichia coli forms obtained by microwave treatment of pure cultures

Gelatin added in certain concentration into the density gradient mixtures allows to solidify and melt the gradient reversibly. Such a method was applied to separation of partially disintegrated Escherichia coli VKM B-125 cells in discontinuous sucrose gradient which was optimized to obtain small rou...

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Veröffentlicht in:	Journal of microbiological methods 2018-10, Vol.153, p.45-47
Hauptverfasser:	Abashina, Tatiana, Vainshtein, Mikhail, Korpela, Saara, Korpela, Timo
Format:	Artikel
Sprache:	eng
Schlagworte:	Centrifugation, Density Gradient - methods Density gradient differential centrifugation Escherichia coli - drug effects Gelatin Microbiological Techniques Microwaves separation of cells and particles solidifying gradients Sucrose
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