DSH5, a dihydrosphingosine C4 hydroxylase gene family member, shows spatially restricted expression in rice and is lethal when expressed ectopically
Dihydrosphingosine C4 hydroxylase (DSH), a diiron-binding membrane enzyme, catalyzes the hydration of dihydrosphingosine and acyl-sphinganine to produce phytosphingosine and phytoceramide, respectively. Rice has two types of DSH homologs: general DSHs, namely DSH1, DSH2 and DSH4, and others that sho...
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| Veröffentlicht in: | Genes & Genetic Systems 2018/08/01, Vol.93(4), pp.135-142 |
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| creator | Imamura, Tomohiro Obata, Chihiro Yoneyama, Kazuyoshi Ichikawa, Masatoshi Ikura, Akane Mutsuro-Aoki, Hiromi Ishikawa, Toshiki Kawai-Yamada, Maki Sasaki, Tadamasa Kusano, Hiroaki Shimada, Hiroaki |
| description | Dihydrosphingosine C4 hydroxylase (DSH), a diiron-binding membrane enzyme, catalyzes the hydration of dihydrosphingosine and acyl-sphinganine to produce phytosphingosine and phytoceramide, respectively. Rice has two types of DSH homologs: general DSHs, namely DSH1, DSH2 and DSH4, and others that show spatial expression profiles, namely DSH3 and DSH5. The general DSHs exist in many plant species. These DSHs showed similarity in their functions and complemented the yeast sur2D mutation. In contrast, homologs of DSH3 and DSH5 were found only in monocot plants. Phylogenetic analysis placed these DSHs in different clades that are evolutionarily divergent from those of the general DSHs. DSH3 and DSH5 showed low-level expression. DSH5 expression was specifically in vascular bundle tissues. Ectopic expression of DSH5 induced a dwarf phenotype characterized by severe growth inhibition and an increase in the thickness of the leaf body caused by enlargement of bulliform cells in the leaves. However, no significant difference was observed in the amount of sphingolipid species. DSH5 did not complement the yeast sur2D mutation, implying that DSH5 has little effect on sphingolipid metabolism. These findings suggested that DSH3 and DSH5 originated and diverged in monocot plants. |
| doi_str_mv | 10.1266/ggs.17-00054 |
| format | Article |
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Rice has two types of DSH homologs: general DSHs, namely DSH1, DSH2 and DSH4, and others that show spatial expression profiles, namely DSH3 and DSH5. The general DSHs exist in many plant species. These DSHs showed similarity in their functions and complemented the yeast sur2D mutation. In contrast, homologs of DSH3 and DSH5 were found only in monocot plants. Phylogenetic analysis placed these DSHs in different clades that are evolutionarily divergent from those of the general DSHs. DSH3 and DSH5 showed low-level expression. DSH5 expression was specifically in vascular bundle tissues. Ectopic expression of DSH5 induced a dwarf phenotype characterized by severe growth inhibition and an increase in the thickness of the leaf body caused by enlargement of bulliform cells in the leaves. However, no significant difference was observed in the amount of sphingolipid species. DSH5 did not complement the yeast sur2D mutation, implying that DSH5 has little effect on sphingolipid metabolism. These findings suggested that DSH3 and DSH5 originated and diverged in monocot plants.</description><identifier>ISSN: 1341-7568</identifier><identifier>EISSN: 1880-5779</identifier><identifier>DOI: 10.1266/ggs.17-00054</identifier><identifier>PMID: 30185720</identifier><language>eng</language><publisher>Japan: The Genetics Society of Japan</publisher><subject>Complement component C4 ; Divergence ; Ectopic expression ; Evolution, Molecular ; evolutionary divergence ; gene family ; Hydroxylase ; Lipid metabolism ; Mixed Function Oxygenases - genetics ; Mixed Function Oxygenases - metabolism ; monocot plant ; Multigene Family ; Mutation ; Oryza - enzymology ; Oryza - genetics ; Phenotypes ; phylogenetic analysis ; Phylogeny ; phytosphingosine ; Plant Leaves - genetics ; Plant Leaves - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; Sphinganine ; Transgenes</subject><ispartof>Genes & Genetic Systems, 2018/08/01, Vol.93(4), pp.135-142</ispartof><rights>2018 by The Genetics Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c496t-22d4f16ba70732044f0abbb67c9b88057973e74be24c717b078b137184b189363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30185720$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Imamura, Tomohiro</creatorcontrib><creatorcontrib>Obata, Chihiro</creatorcontrib><creatorcontrib>Yoneyama, Kazuyoshi</creatorcontrib><creatorcontrib>Ichikawa, Masatoshi</creatorcontrib><creatorcontrib>Ikura, Akane</creatorcontrib><creatorcontrib>Mutsuro-Aoki, Hiromi</creatorcontrib><creatorcontrib>Ishikawa, Toshiki</creatorcontrib><creatorcontrib>Kawai-Yamada, Maki</creatorcontrib><creatorcontrib>Sasaki, Tadamasa</creatorcontrib><creatorcontrib>Kusano, Hiroaki</creatorcontrib><creatorcontrib>Shimada, Hiroaki</creatorcontrib><title>DSH5, a dihydrosphingosine C4 hydroxylase gene family member, shows spatially restricted expression in rice and is lethal when expressed ectopically</title><title>Genes & Genetic Systems</title><addtitle>Genes Genet. Syst.</addtitle><description>Dihydrosphingosine C4 hydroxylase (DSH), a diiron-binding membrane enzyme, catalyzes the hydration of dihydrosphingosine and acyl-sphinganine to produce phytosphingosine and phytoceramide, respectively. Rice has two types of DSH homologs: general DSHs, namely DSH1, DSH2 and DSH4, and others that show spatial expression profiles, namely DSH3 and DSH5. The general DSHs exist in many plant species. These DSHs showed similarity in their functions and complemented the yeast sur2D mutation. In contrast, homologs of DSH3 and DSH5 were found only in monocot plants. Phylogenetic analysis placed these DSHs in different clades that are evolutionarily divergent from those of the general DSHs. DSH3 and DSH5 showed low-level expression. DSH5 expression was specifically in vascular bundle tissues. Ectopic expression of DSH5 induced a dwarf phenotype characterized by severe growth inhibition and an increase in the thickness of the leaf body caused by enlargement of bulliform cells in the leaves. However, no significant difference was observed in the amount of sphingolipid species. DSH5 did not complement the yeast sur2D mutation, implying that DSH5 has little effect on sphingolipid metabolism. These findings suggested that DSH3 and DSH5 originated and diverged in monocot plants.</description><subject>Complement component C4</subject><subject>Divergence</subject><subject>Ectopic expression</subject><subject>Evolution, Molecular</subject><subject>evolutionary divergence</subject><subject>gene family</subject><subject>Hydroxylase</subject><subject>Lipid metabolism</subject><subject>Mixed Function Oxygenases - genetics</subject><subject>Mixed Function Oxygenases - metabolism</subject><subject>monocot plant</subject><subject>Multigene Family</subject><subject>Mutation</subject><subject>Oryza - enzymology</subject><subject>Oryza - genetics</subject><subject>Phenotypes</subject><subject>phylogenetic analysis</subject><subject>Phylogeny</subject><subject>phytosphingosine</subject><subject>Plant Leaves - genetics</subject><subject>Plant Leaves - metabolism</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - metabolism</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>Sphinganine</subject><subject>Transgenes</subject><issn>1341-7568</issn><issn>1880-5779</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkUuP0zAUhSMEYh6wY40ssWHRDH4ldpZMYRikkVgAa8t2bhJXjhPsVDP9H_xg3HbaBZJ1bZ_76ejqnqJ4R_ANoXX9qe_TDRElxrjiL4pLIiUuKyGal_nNOClFVcuL4iqlDcYUN5K9Li4YJrISFF8Wf7_8vK9WSKPWDbs2TmkeXOin5AKgNUcH7WnndQLUQ9Y6PTq_QyOMBuIKpWF6TCjNenHaZz1CWqKzC7QInub8S24KyAWURUA6tMgl5GEZtEePA4QTteftMs3O7m3eFK867RO8fb6vi993X3-t78uHH9--rz8_lJY39VJS2vKO1EYLLBjFnHdYG2NqYRuTt1CJRjAQ3ADlVhBhsJCGMEEkN0Q2rGbXxcej7xynP9s8uhpdsuC9DjBtk6IEY8Y4IzyjH_5DN9M2hjydopTngyWtMrU6UjZvMkXo1BzdqONOEaz2aamcliJCHdLK-Ptn060ZoT3Dp3gycHsENmnRPZwBHRdnPRzcGqb4vpxcz0076KggsH_9w6hw</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>Imamura, Tomohiro</creator><creator>Obata, Chihiro</creator><creator>Yoneyama, Kazuyoshi</creator><creator>Ichikawa, Masatoshi</creator><creator>Ikura, Akane</creator><creator>Mutsuro-Aoki, Hiromi</creator><creator>Ishikawa, Toshiki</creator><creator>Kawai-Yamada, Maki</creator><creator>Sasaki, Tadamasa</creator><creator>Kusano, Hiroaki</creator><creator>Shimada, Hiroaki</creator><general>The Genetics Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7TK</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20180801</creationdate><title>DSH5, a dihydrosphingosine C4 hydroxylase gene family member, shows spatially restricted expression in rice and is lethal when expressed ectopically</title><author>Imamura, Tomohiro ; Obata, Chihiro ; Yoneyama, Kazuyoshi ; Ichikawa, Masatoshi ; Ikura, Akane ; Mutsuro-Aoki, Hiromi ; Ishikawa, Toshiki ; Kawai-Yamada, Maki ; Sasaki, Tadamasa ; Kusano, Hiroaki ; Shimada, Hiroaki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c496t-22d4f16ba70732044f0abbb67c9b88057973e74be24c717b078b137184b189363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Complement component C4</topic><topic>Divergence</topic><topic>Ectopic expression</topic><topic>Evolution, Molecular</topic><topic>evolutionary divergence</topic><topic>gene family</topic><topic>Hydroxylase</topic><topic>Lipid metabolism</topic><topic>Mixed Function Oxygenases - genetics</topic><topic>Mixed Function Oxygenases - metabolism</topic><topic>monocot plant</topic><topic>Multigene Family</topic><topic>Mutation</topic><topic>Oryza - enzymology</topic><topic>Oryza - genetics</topic><topic>Phenotypes</topic><topic>phylogenetic analysis</topic><topic>Phylogeny</topic><topic>phytosphingosine</topic><topic>Plant Leaves - genetics</topic><topic>Plant Leaves - metabolism</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - metabolism</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>Sphinganine</topic><topic>Transgenes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Imamura, Tomohiro</creatorcontrib><creatorcontrib>Obata, Chihiro</creatorcontrib><creatorcontrib>Yoneyama, Kazuyoshi</creatorcontrib><creatorcontrib>Ichikawa, Masatoshi</creatorcontrib><creatorcontrib>Ikura, Akane</creatorcontrib><creatorcontrib>Mutsuro-Aoki, Hiromi</creatorcontrib><creatorcontrib>Ishikawa, Toshiki</creatorcontrib><creatorcontrib>Kawai-Yamada, Maki</creatorcontrib><creatorcontrib>Sasaki, Tadamasa</creatorcontrib><creatorcontrib>Kusano, Hiroaki</creatorcontrib><creatorcontrib>Shimada, Hiroaki</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Neurosciences Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genes & Genetic Systems</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Imamura, Tomohiro</au><au>Obata, Chihiro</au><au>Yoneyama, Kazuyoshi</au><au>Ichikawa, Masatoshi</au><au>Ikura, Akane</au><au>Mutsuro-Aoki, Hiromi</au><au>Ishikawa, Toshiki</au><au>Kawai-Yamada, Maki</au><au>Sasaki, Tadamasa</au><au>Kusano, Hiroaki</au><au>Shimada, Hiroaki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DSH5, a dihydrosphingosine C4 hydroxylase gene family member, shows spatially restricted expression in rice and is lethal when expressed ectopically</atitle><jtitle>Genes & Genetic Systems</jtitle><addtitle>Genes Genet. Syst.</addtitle><date>2018-08-01</date><risdate>2018</risdate><volume>93</volume><issue>4</issue><spage>135</spage><epage>142</epage><pages>135-142</pages><issn>1341-7568</issn><eissn>1880-5779</eissn><abstract>Dihydrosphingosine C4 hydroxylase (DSH), a diiron-binding membrane enzyme, catalyzes the hydration of dihydrosphingosine and acyl-sphinganine to produce phytosphingosine and phytoceramide, respectively. Rice has two types of DSH homologs: general DSHs, namely DSH1, DSH2 and DSH4, and others that show spatial expression profiles, namely DSH3 and DSH5. The general DSHs exist in many plant species. These DSHs showed similarity in their functions and complemented the yeast sur2D mutation. In contrast, homologs of DSH3 and DSH5 were found only in monocot plants. Phylogenetic analysis placed these DSHs in different clades that are evolutionarily divergent from those of the general DSHs. DSH3 and DSH5 showed low-level expression. DSH5 expression was specifically in vascular bundle tissues. Ectopic expression of DSH5 induced a dwarf phenotype characterized by severe growth inhibition and an increase in the thickness of the leaf body caused by enlargement of bulliform cells in the leaves. However, no significant difference was observed in the amount of sphingolipid species. DSH5 did not complement the yeast sur2D mutation, implying that DSH5 has little effect on sphingolipid metabolism. These findings suggested that DSH3 and DSH5 originated and diverged in monocot plants.</abstract><cop>Japan</cop><pub>The Genetics Society of Japan</pub><pmid>30185720</pmid><doi>10.1266/ggs.17-00054</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Complement component C4 Divergence Ectopic expression Evolution, Molecular evolutionary divergence gene family Hydroxylase Lipid metabolism Mixed Function Oxygenases - genetics Mixed Function Oxygenases - metabolism monocot plant Multigene Family Mutation Oryza - enzymology Oryza - genetics Phenotypes phylogenetic analysis Phylogeny phytosphingosine Plant Leaves - genetics Plant Leaves - metabolism Plant Proteins - genetics Plant Proteins - metabolism Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Sphinganine Transgenes |
| title | DSH5, a dihydrosphingosine C4 hydroxylase gene family member, shows spatially restricted expression in rice and is lethal when expressed ectopically |
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