Quercetin attenuates Ox-LDL-induced calcification in vascular smooth muscle cells by regulating ROS-TLR4 signaling pathway
To determine whether quercetin inhibits oxidized low-density lipoprotein (Ox-LDL)-induced osteogenic differentiation and calcification of vascular smooth muscle cells (VSMCs) and understand the underlying mechanism. The calcification of human VSMCs following Ox-LDL treatment was assessed using aliza...
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| Veröffentlicht in: | Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2018-07, Vol.38 (8), p.980 |
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| container_title | Nan fang yi ke da xue xue bao = Journal of Southern Medical University |
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| creator | Liang, Qingchun Chen, Yanting Li, Chuanxiang Lu, Lihe |
| description | To determine whether quercetin inhibits oxidized low-density lipoprotein (Ox-LDL)-induced osteogenic differentiation and calcification of vascular smooth muscle cells (VSMCs) and understand the underlying mechanism.
The calcification of human VSMCs following Ox-LDL treatment was assessed using alizarin red staining and by detecting ALP activity. The mRNA expressions of the bone-related genes including Msx2, BMP2 and Osterix, and the contractile proteins including SMA and SM22a were analyzed using qPCR. The effects of quercetin were investigated on OxLDL-induced VSMC calcification and changes in ALP activity, expressions of Msx2, BMP2, Osterix, SMA and SM22a, ROS levels and SOD activity. The effect of Toll like receptor 4 (TLR4) silencing mediated by siRNA transfection on cell calcification, ALP activity, gene expressions and ROS levels were investigated.
Ox-LDL treatment promoted VSMC calcification and up-regulated TLR4 expression. Quercetin treatment significantly attenuated Ox-LDL-induced VSMC calcification, reduced ALP activity, down-regulated the expression levels of Msx2, BMP2 and Osterix, and up-regulated the expressions of vascular smooth muscle contractile proteins SMA and SM22a. In addition, Quercetin treatment markedly increased SOD activity, reduced ROS levels and TLR4 expression in VSMCs. Silencing TLR4 expression using TLR4 siRNA also significantly decreased calcification of the VSMCs.
Quercetin inhibits Ox-LDL-induced VSMC calcification in VSMCs possibly by targeting the ROS/TLR4 signaling pathway. |
| doi_str_mv | 10.3969/j.issn.1673-4254.2018.08.13 |
| format | Article |
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The calcification of human VSMCs following Ox-LDL treatment was assessed using alizarin red staining and by detecting ALP activity. The mRNA expressions of the bone-related genes including Msx2, BMP2 and Osterix, and the contractile proteins including SMA and SM22a were analyzed using qPCR. The effects of quercetin were investigated on OxLDL-induced VSMC calcification and changes in ALP activity, expressions of Msx2, BMP2, Osterix, SMA and SM22a, ROS levels and SOD activity. The effect of Toll like receptor 4 (TLR4) silencing mediated by siRNA transfection on cell calcification, ALP activity, gene expressions and ROS levels were investigated.
Ox-LDL treatment promoted VSMC calcification and up-regulated TLR4 expression. Quercetin treatment significantly attenuated Ox-LDL-induced VSMC calcification, reduced ALP activity, down-regulated the expression levels of Msx2, BMP2 and Osterix, and up-regulated the expressions of vascular smooth muscle contractile proteins SMA and SM22a. In addition, Quercetin treatment markedly increased SOD activity, reduced ROS levels and TLR4 expression in VSMCs. Silencing TLR4 expression using TLR4 siRNA also significantly decreased calcification of the VSMCs.
Quercetin inhibits Ox-LDL-induced VSMC calcification in VSMCs possibly by targeting the ROS/TLR4 signaling pathway.</description><identifier>ISSN: 1673-4254</identifier><identifier>DOI: 10.3969/j.issn.1673-4254.2018.08.13</identifier><identifier>PMID: 30187880</identifier><language>chi</language><publisher>China</publisher><subject>Actins - genetics ; Actins - metabolism ; Bone Morphogenetic Protein 2 - genetics ; Bone Morphogenetic Protein 2 - metabolism ; Cell Differentiation - drug effects ; Cells, Cultured ; Homeodomain Proteins - genetics ; Homeodomain Proteins - metabolism ; Humans ; Lipoproteins, LDL - antagonists & inhibitors ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - drug effects ; Myocytes, Smooth Muscle - drug effects ; Osteogenesis - genetics ; Quercetin - pharmacology ; Reactive Oxygen Species - metabolism ; RNA, Messenger - metabolism ; RNA, Small Interfering ; Sp7 Transcription Factor - genetics ; Sp7 Transcription Factor - metabolism ; Toll-Like Receptor 4 - metabolism ; Vascular Calcification - drug therapy</subject><ispartof>Nan fang yi ke da xue xue bao = Journal of Southern Medical University, 2018-07, Vol.38 (8), p.980</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c184t-c72e8695e76ba4580e579d943ccf848f51b369acdfbe980b3e716f48247308303</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30187880$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Liang, Qingchun</creatorcontrib><creatorcontrib>Chen, Yanting</creatorcontrib><creatorcontrib>Li, Chuanxiang</creatorcontrib><creatorcontrib>Lu, Lihe</creatorcontrib><title>Quercetin attenuates Ox-LDL-induced calcification in vascular smooth muscle cells by regulating ROS-TLR4 signaling pathway</title><title>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</title><addtitle>Nan Fang Yi Ke Da Xue Xue Bao</addtitle><description>To determine whether quercetin inhibits oxidized low-density lipoprotein (Ox-LDL)-induced osteogenic differentiation and calcification of vascular smooth muscle cells (VSMCs) and understand the underlying mechanism.
The calcification of human VSMCs following Ox-LDL treatment was assessed using alizarin red staining and by detecting ALP activity. The mRNA expressions of the bone-related genes including Msx2, BMP2 and Osterix, and the contractile proteins including SMA and SM22a were analyzed using qPCR. The effects of quercetin were investigated on OxLDL-induced VSMC calcification and changes in ALP activity, expressions of Msx2, BMP2, Osterix, SMA and SM22a, ROS levels and SOD activity. The effect of Toll like receptor 4 (TLR4) silencing mediated by siRNA transfection on cell calcification, ALP activity, gene expressions and ROS levels were investigated.
Ox-LDL treatment promoted VSMC calcification and up-regulated TLR4 expression. Quercetin treatment significantly attenuated Ox-LDL-induced VSMC calcification, reduced ALP activity, down-regulated the expression levels of Msx2, BMP2 and Osterix, and up-regulated the expressions of vascular smooth muscle contractile proteins SMA and SM22a. In addition, Quercetin treatment markedly increased SOD activity, reduced ROS levels and TLR4 expression in VSMCs. Silencing TLR4 expression using TLR4 siRNA also significantly decreased calcification of the VSMCs.
Quercetin inhibits Ox-LDL-induced VSMC calcification in VSMCs possibly by targeting the ROS/TLR4 signaling pathway.</description><subject>Actins - genetics</subject><subject>Actins - metabolism</subject><subject>Bone Morphogenetic Protein 2 - genetics</subject><subject>Bone Morphogenetic Protein 2 - metabolism</subject><subject>Cell Differentiation - drug effects</subject><subject>Cells, Cultured</subject><subject>Homeodomain Proteins - genetics</subject><subject>Homeodomain Proteins - metabolism</subject><subject>Humans</subject><subject>Lipoproteins, LDL - antagonists & inhibitors</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Myocytes, Smooth Muscle - drug effects</subject><subject>Osteogenesis - genetics</subject><subject>Quercetin - pharmacology</subject><subject>Reactive Oxygen Species - metabolism</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Small Interfering</subject><subject>Sp7 Transcription Factor - genetics</subject><subject>Sp7 Transcription Factor - metabolism</subject><subject>Toll-Like Receptor 4 - metabolism</subject><subject>Vascular Calcification - drug therapy</subject><issn>1673-4254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kF1LwzAYhXOhuDH3FyTgjTetSZM26aXMTygM57wuSfp2i_RjNok6f70dTq8OnPNwOByELimJWZ7l12-xda6LaSZYxJOUxwmhMiYypuwETf_tCZo7ZzVJKRMkzcgZmrARFFKSKfp-DjAY8LbDynvogvLg8PIrKm6LyHZVMFBhoxpja2uUt32HR_RDORMaNWDX9r3f4jY40wA20DQO6z0eYDPGY-kGr5Yv0bpYcezsplPNwdopv_1U-3N0WqvGwfyoM_R6f7dePEbF8uFpcVNEhkruIyMSkFmegsi04qkkkIq8yjkzppZc1inVLMuVqWoNuSSagaBZzWXCBSOSETZDV7-9u6F_D-B82Vp3mKo66IMrE0oIS0TC5YheHNGgW6jK3WBbNezLv7_YDy5Qb8Y</recordid><startdate>20180730</startdate><enddate>20180730</enddate><creator>Liang, Qingchun</creator><creator>Chen, Yanting</creator><creator>Li, Chuanxiang</creator><creator>Lu, Lihe</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20180730</creationdate><title>Quercetin attenuates Ox-LDL-induced calcification in vascular smooth muscle cells by regulating ROS-TLR4 signaling pathway</title><author>Liang, Qingchun ; Chen, Yanting ; Li, Chuanxiang ; Lu, Lihe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c184t-c72e8695e76ba4580e579d943ccf848f51b369acdfbe980b3e716f48247308303</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>chi</language><creationdate>2018</creationdate><topic>Actins - genetics</topic><topic>Actins - metabolism</topic><topic>Bone Morphogenetic Protein 2 - genetics</topic><topic>Bone Morphogenetic Protein 2 - metabolism</topic><topic>Cell Differentiation - drug effects</topic><topic>Cells, Cultured</topic><topic>Homeodomain Proteins - genetics</topic><topic>Homeodomain Proteins - metabolism</topic><topic>Humans</topic><topic>Lipoproteins, LDL - antagonists & inhibitors</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Myocytes, Smooth Muscle - drug effects</topic><topic>Osteogenesis - genetics</topic><topic>Quercetin - pharmacology</topic><topic>Reactive Oxygen Species - metabolism</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Small Interfering</topic><topic>Sp7 Transcription Factor - genetics</topic><topic>Sp7 Transcription Factor - metabolism</topic><topic>Toll-Like Receptor 4 - metabolism</topic><topic>Vascular Calcification - drug therapy</topic><toplevel>online_resources</toplevel><creatorcontrib>Liang, Qingchun</creatorcontrib><creatorcontrib>Chen, Yanting</creatorcontrib><creatorcontrib>Li, Chuanxiang</creatorcontrib><creatorcontrib>Lu, Lihe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Liang, Qingchun</au><au>Chen, Yanting</au><au>Li, Chuanxiang</au><au>Lu, Lihe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quercetin attenuates Ox-LDL-induced calcification in vascular smooth muscle cells by regulating ROS-TLR4 signaling pathway</atitle><jtitle>Nan fang yi ke da xue xue bao = Journal of Southern Medical University</jtitle><addtitle>Nan Fang Yi Ke Da Xue Xue Bao</addtitle><date>2018-07-30</date><risdate>2018</risdate><volume>38</volume><issue>8</issue><spage>980</spage><pages>980-</pages><issn>1673-4254</issn><abstract>To determine whether quercetin inhibits oxidized low-density lipoprotein (Ox-LDL)-induced osteogenic differentiation and calcification of vascular smooth muscle cells (VSMCs) and understand the underlying mechanism.
The calcification of human VSMCs following Ox-LDL treatment was assessed using alizarin red staining and by detecting ALP activity. The mRNA expressions of the bone-related genes including Msx2, BMP2 and Osterix, and the contractile proteins including SMA and SM22a were analyzed using qPCR. The effects of quercetin were investigated on OxLDL-induced VSMC calcification and changes in ALP activity, expressions of Msx2, BMP2, Osterix, SMA and SM22a, ROS levels and SOD activity. The effect of Toll like receptor 4 (TLR4) silencing mediated by siRNA transfection on cell calcification, ALP activity, gene expressions and ROS levels were investigated.
Ox-LDL treatment promoted VSMC calcification and up-regulated TLR4 expression. Quercetin treatment significantly attenuated Ox-LDL-induced VSMC calcification, reduced ALP activity, down-regulated the expression levels of Msx2, BMP2 and Osterix, and up-regulated the expressions of vascular smooth muscle contractile proteins SMA and SM22a. In addition, Quercetin treatment markedly increased SOD activity, reduced ROS levels and TLR4 expression in VSMCs. Silencing TLR4 expression using TLR4 siRNA also significantly decreased calcification of the VSMCs.
Quercetin inhibits Ox-LDL-induced VSMC calcification in VSMCs possibly by targeting the ROS/TLR4 signaling pathway.</abstract><cop>China</cop><pmid>30187880</pmid><doi>10.3969/j.issn.1673-4254.2018.08.13</doi></addata></record> |
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| subjects | Actins - genetics Actins - metabolism Bone Morphogenetic Protein 2 - genetics Bone Morphogenetic Protein 2 - metabolism Cell Differentiation - drug effects Cells, Cultured Homeodomain Proteins - genetics Homeodomain Proteins - metabolism Humans Lipoproteins, LDL - antagonists & inhibitors Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Myocytes, Smooth Muscle - drug effects Osteogenesis - genetics Quercetin - pharmacology Reactive Oxygen Species - metabolism RNA, Messenger - metabolism RNA, Small Interfering Sp7 Transcription Factor - genetics Sp7 Transcription Factor - metabolism Toll-Like Receptor 4 - metabolism Vascular Calcification - drug therapy |
| title | Quercetin attenuates Ox-LDL-induced calcification in vascular smooth muscle cells by regulating ROS-TLR4 signaling pathway |
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