Killed Bifidobacterium longum enhanced stress tolerance and prolonged life span of Caenorhabditis elegans via DAF-16
Probiotics are bacteria among the intestinal flora that are beneficial for human health. Bifidobacterium longum (BL) is a prototypical probiotic that is widely used in yogurt making, supplements and others. Although various physiological effects of BL have been reported, those associated with longev...
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| Veröffentlicht in: | British journal of nutrition 2018-10, Vol.120 (8), p.872-880 |
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| description | Probiotics are bacteria among the intestinal flora that are beneficial for human health. Bifidobacterium longum (BL) is a prototypical probiotic that is widely used in yogurt making, supplements and others. Although various physiological effects of BL have been reported, those associated with longevity and anti-ageing still remain elusive. Here we aimed to elucidate the physiological effects of killed BL (BR-108) on stress tolerance and longevity of Caenorhabditis elegans and their mechanisms. Worms fed killed BL in addition to Escherichia coli (OP50) displayed reduced body length in a BL dose-dependent manner. When compared with those fed E. coli alone, these worms had a higher survival rate following heat stress at 35°C and hydrogen peroxide-induced oxidative stress. A general decrease in motility was observed over time in all worms; however, killed BL-fed ageing worms displayed increased movement and longer life span than those fed E. coli alone. However, the longevity effect was suppressed in sir-2.1, daf-16 and skn-1-deficient worms. Killed BL induced DAF-16 nuclear localisation and increased the expression of the DAF-16 target gene hsp-12.6. These results revealed that the physiological effects of killed BL in C. elegans were mediated by DAF-16 activation. These findings contradict previous observations with different Bifidobacterium and Lactobacillus strains, which showed the role for SKN-1 independently of DAF-16. |
| doi_str_mv | 10.1017/S0007114518001563 |
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Bifidobacterium longum (BL) is a prototypical probiotic that is widely used in yogurt making, supplements and others. Although various physiological effects of BL have been reported, those associated with longevity and anti-ageing still remain elusive. Here we aimed to elucidate the physiological effects of killed BL (BR-108) on stress tolerance and longevity of Caenorhabditis elegans and their mechanisms. Worms fed killed BL in addition to Escherichia coli (OP50) displayed reduced body length in a BL dose-dependent manner. When compared with those fed E. coli alone, these worms had a higher survival rate following heat stress at 35°C and hydrogen peroxide-induced oxidative stress. A general decrease in motility was observed over time in all worms; however, killed BL-fed ageing worms displayed increased movement and longer life span than those fed E. coli alone. However, the longevity effect was suppressed in sir-2.1, daf-16 and skn-1-deficient worms. Killed BL induced DAF-16 nuclear localisation and increased the expression of the DAF-16 target gene hsp-12.6. These results revealed that the physiological effects of killed BL in C. elegans were mediated by DAF-16 activation. These findings contradict previous observations with different Bifidobacterium and Lactobacillus strains, which showed the role for SKN-1 independently of DAF-16.</description><identifier>ISSN: 0007-1145</identifier><identifier>EISSN: 1475-2662</identifier><identifier>DOI: 10.1017/S0007114518001563</identifier><identifier>PMID: 30178731</identifier><language>eng</language><publisher>Cambridge, UK: Cambridge University Press</publisher><subject>Aging ; Bifidobacterium longum ; Body length ; Caenorhabditis elegans ; E coli ; Escherichia coli ; Flora ; Gene expression ; Heat shock proteins ; Heat stress ; Heat tolerance ; Hydrogen peroxide ; Insects ; Intestinal microflora ; Intestine ; Life span ; Longevity ; Microbiology ; Nematodes ; Oxidative stress ; Physiological effects ; Physiology ; Probiotics ; Survival ; Transcription factors ; Worms ; Yogurt</subject><ispartof>British journal of nutrition, 2018-10, Vol.120 (8), p.872-880</ispartof><rights>The Authors 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c574t-46b046ffb981dbdc83a02b79c68ad12e45d91f3e597d1512ae85d9ff95c74cd93</citedby><cites>FETCH-LOGICAL-c574t-46b046ffb981dbdc83a02b79c68ad12e45d91f3e597d1512ae85d9ff95c74cd93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.cambridge.org/core/product/identifier/S0007114518001563/type/journal_article$$EHTML$$P50$$Gcambridge$$H</linktohtml><link.rule.ids>164,314,780,784,27924,27925,55628</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30178731$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sugawara, Takaya</creatorcontrib><creatorcontrib>Sakamoto, Kazuichi</creatorcontrib><title>Killed Bifidobacterium longum enhanced stress tolerance and prolonged life span of Caenorhabditis elegans via DAF-16</title><title>British journal of nutrition</title><addtitle>Br J Nutr</addtitle><description>Probiotics are bacteria among the intestinal flora that are beneficial for human health. Bifidobacterium longum (BL) is a prototypical probiotic that is widely used in yogurt making, supplements and others. Although various physiological effects of BL have been reported, those associated with longevity and anti-ageing still remain elusive. Here we aimed to elucidate the physiological effects of killed BL (BR-108) on stress tolerance and longevity of Caenorhabditis elegans and their mechanisms. Worms fed killed BL in addition to Escherichia coli (OP50) displayed reduced body length in a BL dose-dependent manner. When compared with those fed E. coli alone, these worms had a higher survival rate following heat stress at 35°C and hydrogen peroxide-induced oxidative stress. A general decrease in motility was observed over time in all worms; however, killed BL-fed ageing worms displayed increased movement and longer life span than those fed E. coli alone. However, the longevity effect was suppressed in sir-2.1, daf-16 and skn-1-deficient worms. Killed BL induced DAF-16 nuclear localisation and increased the expression of the DAF-16 target gene hsp-12.6. These results revealed that the physiological effects of killed BL in C. elegans were mediated by DAF-16 activation. These findings contradict previous observations with different Bifidobacterium and Lactobacillus strains, which showed the role for SKN-1 independently of DAF-16.</description><subject>Aging</subject><subject>Bifidobacterium longum</subject><subject>Body length</subject><subject>Caenorhabditis elegans</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Flora</subject><subject>Gene expression</subject><subject>Heat shock proteins</subject><subject>Heat stress</subject><subject>Heat tolerance</subject><subject>Hydrogen peroxide</subject><subject>Insects</subject><subject>Intestinal microflora</subject><subject>Intestine</subject><subject>Life span</subject><subject>Longevity</subject><subject>Microbiology</subject><subject>Nematodes</subject><subject>Oxidative stress</subject><subject>Physiological effects</subject><subject>Physiology</subject><subject>Probiotics</subject><subject>Survival</subject><subject>Transcription factors</subject><subject>Worms</subject><subject>Yogurt</subject><issn>0007-1145</issn><issn>1475-2662</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kUFv1DAQhS0EotvCD-CCLHHhEvDEcWwfy0ILohIH4Bw59njryokXO0Hi3-OoC0ggTiPP-97zSI-QZ8BeAQP5-jNjTAJ0AhRjIHr-gOygk6Jp-759SHab3Gz6GTkv5a4-FTD9mJzx6laSw44sH0OM6Oib4INLo7EL5rBONKb5UAfOt2a2VS9LxlLokiLmbUPN7Ogxp42rcgweaTmamSZP9wbnlG_N6MISCsWIBzMX-j0Y-vbyqoH-CXnkTSz49DQvyNerd1_275ubT9cf9pc3jRWyW5quH1nXez9qBW50VnHD2lFq2yvjoMVOOA2eo9DSgYDWoKob77WwsrNO8wvy8j63HvptxbIMUygWYzQzprUMLdO64xxAVPTFX-hdWvNcrxtagFYp2UteKbinbE6lZPTDMYfJ5B8DsGGrZPinkup5fkpexwndb8evDirAT6FmGnNwB_zz9_9jfwJRpZVv</recordid><startdate>20181028</startdate><enddate>20181028</enddate><creator>Sugawara, Takaya</creator><creator>Sakamoto, Kazuichi</creator><general>Cambridge University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7RV</scope><scope>7T5</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AN0</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20181028</creationdate><title>Killed Bifidobacterium longum enhanced stress tolerance and prolonged life span of Caenorhabditis elegans via DAF-16</title><author>Sugawara, Takaya ; Sakamoto, Kazuichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c574t-46b046ffb981dbdc83a02b79c68ad12e45d91f3e597d1512ae85d9ff95c74cd93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Aging</topic><topic>Bifidobacterium longum</topic><topic>Body length</topic><topic>Caenorhabditis elegans</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Flora</topic><topic>Gene expression</topic><topic>Heat shock proteins</topic><topic>Heat stress</topic><topic>Heat tolerance</topic><topic>Hydrogen peroxide</topic><topic>Insects</topic><topic>Intestinal microflora</topic><topic>Intestine</topic><topic>Life span</topic><topic>Longevity</topic><topic>Microbiology</topic><topic>Nematodes</topic><topic>Oxidative stress</topic><topic>Physiological effects</topic><topic>Physiology</topic><topic>Probiotics</topic><topic>Survival</topic><topic>Transcription factors</topic><topic>Worms</topic><topic>Yogurt</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sugawara, Takaya</creatorcontrib><creatorcontrib>Sakamoto, Kazuichi</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Proquest Nursing & Allied Health Source</collection><collection>Immunology Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>British Nursing Database</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Research Library (Corporate)</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>British journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sugawara, Takaya</au><au>Sakamoto, Kazuichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Killed Bifidobacterium longum enhanced stress tolerance and prolonged life span of Caenorhabditis elegans via DAF-16</atitle><jtitle>British journal of nutrition</jtitle><addtitle>Br J Nutr</addtitle><date>2018-10-28</date><risdate>2018</risdate><volume>120</volume><issue>8</issue><spage>872</spage><epage>880</epage><pages>872-880</pages><issn>0007-1145</issn><eissn>1475-2662</eissn><abstract>Probiotics are bacteria among the intestinal flora that are beneficial for human health. Bifidobacterium longum (BL) is a prototypical probiotic that is widely used in yogurt making, supplements and others. Although various physiological effects of BL have been reported, those associated with longevity and anti-ageing still remain elusive. Here we aimed to elucidate the physiological effects of killed BL (BR-108) on stress tolerance and longevity of Caenorhabditis elegans and their mechanisms. Worms fed killed BL in addition to Escherichia coli (OP50) displayed reduced body length in a BL dose-dependent manner. When compared with those fed E. coli alone, these worms had a higher survival rate following heat stress at 35°C and hydrogen peroxide-induced oxidative stress. A general decrease in motility was observed over time in all worms; however, killed BL-fed ageing worms displayed increased movement and longer life span than those fed E. coli alone. However, the longevity effect was suppressed in sir-2.1, daf-16 and skn-1-deficient worms. Killed BL induced DAF-16 nuclear localisation and increased the expression of the DAF-16 target gene hsp-12.6. These results revealed that the physiological effects of killed BL in C. elegans were mediated by DAF-16 activation. These findings contradict previous observations with different Bifidobacterium and Lactobacillus strains, which showed the role for SKN-1 independently of DAF-16.</abstract><cop>Cambridge, UK</cop><pub>Cambridge University Press</pub><pmid>30178731</pmid><doi>10.1017/S0007114518001563</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Aging Bifidobacterium longum Body length Caenorhabditis elegans E coli Escherichia coli Flora Gene expression Heat shock proteins Heat stress Heat tolerance Hydrogen peroxide Insects Intestinal microflora Intestine Life span Longevity Microbiology Nematodes Oxidative stress Physiological effects Physiology Probiotics Survival Transcription factors Worms Yogurt |
| title | Killed Bifidobacterium longum enhanced stress tolerance and prolonged life span of Caenorhabditis elegans via DAF-16 |
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