The use of enzyme-linked immunosorbent assays (ELISA) for the determination of pollutants in environmental and industrial wastes

Twelve enzyme-linked immunosorbent assays (ELISA), for the determination of surfactants [linear alkylbenzene sulfonates (LAS), alkyl ethoxylates (AE), and alkylphenol ethoxylates (APE)], endocrine disruptors [alkylphenol (AP), AP + APE, and bisphenol A (BPA)], estrogens [17beta-estradiol (E2), estro...

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Veröffentlicht in:Water science and technology 2006-01, Vol.54 (11-12), p.1-9
Hauptverfasser: Hirobe, M, Goda, Y, Okayasu, Y, Tomita, J, Takigami, H, Ike, M, Tanaka, H
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container_end_page 9
container_issue 11-12
container_start_page 1
container_title Water science and technology
container_volume 54
creator Hirobe, M
Goda, Y
Okayasu, Y
Tomita, J
Takigami, H
Ike, M
Tanaka, H
description Twelve enzyme-linked immunosorbent assays (ELISA), for the determination of surfactants [linear alkylbenzene sulfonates (LAS), alkyl ethoxylates (AE), and alkylphenol ethoxylates (APE)], endocrine disruptors [alkylphenol (AP), AP + APE, and bisphenol A (BPA)], estrogens [17beta-estradiol (E2), estrone (El), estrogen (ES: El + E2 + estriol (E3)), 1 7alfa-ethynylestradiol (EE2)], dioxins and polychlorinated biphenyls (PCBs), were validated on environmental water and industrial wastes. The lowest quantification limits of these ELISAs were 0.05 microg/L (BPA, E2, El, ES and EE2), 2 microg/L (AE), 3 microg/L (dioxins and PCBs), 5 microg/L (AP, AP + APE) and 20 microg/L (LAS and APE). To apply these ELISAs to environmental or industrial waste samples, simple and appropriate pre-treatment methods were also developed for each ELISA. With optimized pre-treatments, the values of ELISAs were well co-related, in all cases, to those of instrumental analytical methods such as liquid chromatography (HPLC), liquid chromatography-tandem mass spectrometry (LC-MS/MS), and high-resolution gas chromatography mass spectrometry (HR-GC-MS), etc.
doi_str_mv 10.2166/wst.2006.735
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subjects 17β-Estradiol
Alkylbenzene sulfonate
Alkylphenol ethoxylates
Bisphenol A
Chemical pollutants
Chlorinated hydrocarbons
Chromatography
Dioxins
Dioxins - analysis
ELISA
Endocrine disruptors
Environmental Pollutants - analysis
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Enzymes
Estrogens
Estrogens - analysis
Fresh Water
Gas chromatography
High performance liquid chromatography
HPLC
Immunoassays
Industrial pollution
Industrial Waste - analysis
Industrial wastes
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Oestrogens
PCB
Pollutants
Polychlorinated biphenyls
Polychlorinated Biphenyls - analysis
Pretreatment
Scientific imaging
Seawater
Sex hormones
Spectroscopy
Sulfonates
Water Pollutants - analysis
title The use of enzyme-linked immunosorbent assays (ELISA) for the determination of pollutants in environmental and industrial wastes
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