Co-infection with porcine bocavirus and porcine circovirus 2 affects inflammatory cytokine production and tight junctions of IPEC-J2 cells
Porcine bocavirus (PBoV) has a high prevalence in both healthy and diseased swine around the world. It was recently reported that PBoV and porcine circovirus type 2 (PCV2)—which contribute to porcine diarrheal disease—have a high rate of co-infection. To clarify the pathogenesis of PBoV, we examined...
Gespeichert in:
| Veröffentlicht in: | Virus genes 2018-10, Vol.54 (5), p.684-693 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 693 |
|---|---|
| container_issue | 5 |
| container_start_page | 684 |
| container_title | Virus genes |
| container_volume | 54 |
| creator | Zhang, Jing Lu, Yangchao Li, Shaowen Ku, Xugang Liu, Xiaoli Memon, Atta Muhammad He, Qigai Bi, Dingren Meng, Xianrong |
| description | Porcine bocavirus (PBoV) has a high prevalence in both healthy and diseased swine around the world. It was recently reported that PBoV and porcine circovirus type 2 (PCV2)—which contribute to porcine diarrheal disease—have a high rate of co-infection. To clarify the pathogenesis of PBoV, we examined the co-infection rate and effects of these two pathogens in IPEC-J2 porcine intestinal enterocytes. Both single and co-infection had cytopathic effects in IPEC-J2 cells. The apoptosis and proliferation rates of cells infected with both viruses did not differ significantly from those of cells infected with either one alone. PBoV and PCV2 induced the upregulation of inflammatory cytokines and the downregulation of the tight junction proteins occludin and claudin 1 in the early stage of infection, leading to destruction of epithelial barrier integrity and enhanced cytotoxicity. These findings provide insight into the pathogenic mechanisms of PBoV and PCV2 and a basis for developing effective strategies to prevent the spread of gastrointestinal diseases in pigs and other livestock. |
| doi_str_mv | 10.1007/s11262-018-1596-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2099043530</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2098292240</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-6f008f9bfd306d2cd3e6cc196ab0b1abf9ee50e938e8fa45f4bf1d4c4910b1463</originalsourceid><addsrcrecordid>eNp1kU9rFTEUxUNR2mfbD9CNBNy4id78mbzJUh5VKwVdKHQXMpmkzfPN5JnMKO8r-KlNnLZCwVXg3N89OZeD0AWFNxRg_TZTyiQjQFtCGyWJPEIr2qwZUUrcPEMrUAxI20h1gl7kvAWAtmXiGJ1woGvOJV-h35tIwuidnUIc8a8w3eF9TDaMDnfRmp8hzRmbsX9UbUg2LjLDxtfNjIvDzgyDmWI6YHuY4veK7lPs58W4Okzh9m7C23n8K2UcPb76crkhnxi2brfLZ-i5N7vszu_fU_Tt_eXXzUdy_fnD1ebdNbF8zSYifTnDq873HGTPbM-dtJYqaTroqOm8cq4Bp3jrWm9E40XnaS-sULTMheSn6PXiW_L9mF2e9BByTWBGF-esGSgFgjccCvrqCbqNcxpLukq1TDEmKkUXyqaYc3Je71MYTDpoCroWpZeidClK16J0DfHy3nnuBtc_bjw0UwC2ALmMxluX_n39f9c_xX2gOg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2098292240</pqid></control><display><type>article</type><title>Co-infection with porcine bocavirus and porcine circovirus 2 affects inflammatory cytokine production and tight junctions of IPEC-J2 cells</title><source>MEDLINE</source><source>SpringerNature Journals</source><creator>Zhang, Jing ; Lu, Yangchao ; Li, Shaowen ; Ku, Xugang ; Liu, Xiaoli ; Memon, Atta Muhammad ; He, Qigai ; Bi, Dingren ; Meng, Xianrong</creator><creatorcontrib>Zhang, Jing ; Lu, Yangchao ; Li, Shaowen ; Ku, Xugang ; Liu, Xiaoli ; Memon, Atta Muhammad ; He, Qigai ; Bi, Dingren ; Meng, Xianrong</creatorcontrib><description>Porcine bocavirus (PBoV) has a high prevalence in both healthy and diseased swine around the world. It was recently reported that PBoV and porcine circovirus type 2 (PCV2)—which contribute to porcine diarrheal disease—have a high rate of co-infection. To clarify the pathogenesis of PBoV, we examined the co-infection rate and effects of these two pathogens in IPEC-J2 porcine intestinal enterocytes. Both single and co-infection had cytopathic effects in IPEC-J2 cells. The apoptosis and proliferation rates of cells infected with both viruses did not differ significantly from those of cells infected with either one alone. PBoV and PCV2 induced the upregulation of inflammatory cytokines and the downregulation of the tight junction proteins occludin and claudin 1 in the early stage of infection, leading to destruction of epithelial barrier integrity and enhanced cytotoxicity. These findings provide insight into the pathogenic mechanisms of PBoV and PCV2 and a basis for developing effective strategies to prevent the spread of gastrointestinal diseases in pigs and other livestock.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-018-1596-6</identifier><identifier>PMID: 30173363</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Animals ; Apoptosis ; Biomedical and Life Sciences ; Biomedicine ; Bocavirus - pathogenicity ; Cell Line ; Cell proliferation ; Circoviridae Infections - prevention & control ; Circoviridae Infections - virology ; Circovirus - pathogenicity ; Coinfection ; Cytokines ; Cytokines - biosynthesis ; Cytopathogenic Effect, Viral ; Cytotoxicity ; Diarrhea ; Enterocytes ; Gastrointestinal diseases ; Infections ; Inflammation ; Intestine ; Livestock ; Medical Microbiology ; Parvoviridae Infections - virology ; Plant Sciences ; Swine ; Swine Diseases - pathology ; Swine Diseases - prevention & control ; Swine Diseases - virology ; Tight junctions ; Tight Junctions - virology ; Virology ; Virus Replication</subject><ispartof>Virus genes, 2018-10, Vol.54 (5), p.684-693</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2018</rights><rights>Virus Genes is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-6f008f9bfd306d2cd3e6cc196ab0b1abf9ee50e938e8fa45f4bf1d4c4910b1463</citedby><cites>FETCH-LOGICAL-c372t-6f008f9bfd306d2cd3e6cc196ab0b1abf9ee50e938e8fa45f4bf1d4c4910b1463</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11262-018-1596-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11262-018-1596-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27926,27927,41490,42559,51321</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30173363$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Lu, Yangchao</creatorcontrib><creatorcontrib>Li, Shaowen</creatorcontrib><creatorcontrib>Ku, Xugang</creatorcontrib><creatorcontrib>Liu, Xiaoli</creatorcontrib><creatorcontrib>Memon, Atta Muhammad</creatorcontrib><creatorcontrib>He, Qigai</creatorcontrib><creatorcontrib>Bi, Dingren</creatorcontrib><creatorcontrib>Meng, Xianrong</creatorcontrib><title>Co-infection with porcine bocavirus and porcine circovirus 2 affects inflammatory cytokine production and tight junctions of IPEC-J2 cells</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><addtitle>Virus Genes</addtitle><description>Porcine bocavirus (PBoV) has a high prevalence in both healthy and diseased swine around the world. It was recently reported that PBoV and porcine circovirus type 2 (PCV2)—which contribute to porcine diarrheal disease—have a high rate of co-infection. To clarify the pathogenesis of PBoV, we examined the co-infection rate and effects of these two pathogens in IPEC-J2 porcine intestinal enterocytes. Both single and co-infection had cytopathic effects in IPEC-J2 cells. The apoptosis and proliferation rates of cells infected with both viruses did not differ significantly from those of cells infected with either one alone. PBoV and PCV2 induced the upregulation of inflammatory cytokines and the downregulation of the tight junction proteins occludin and claudin 1 in the early stage of infection, leading to destruction of epithelial barrier integrity and enhanced cytotoxicity. These findings provide insight into the pathogenic mechanisms of PBoV and PCV2 and a basis for developing effective strategies to prevent the spread of gastrointestinal diseases in pigs and other livestock.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Bocavirus - pathogenicity</subject><subject>Cell Line</subject><subject>Cell proliferation</subject><subject>Circoviridae Infections - prevention & control</subject><subject>Circoviridae Infections - virology</subject><subject>Circovirus - pathogenicity</subject><subject>Coinfection</subject><subject>Cytokines</subject><subject>Cytokines - biosynthesis</subject><subject>Cytopathogenic Effect, Viral</subject><subject>Cytotoxicity</subject><subject>Diarrhea</subject><subject>Enterocytes</subject><subject>Gastrointestinal diseases</subject><subject>Infections</subject><subject>Inflammation</subject><subject>Intestine</subject><subject>Livestock</subject><subject>Medical Microbiology</subject><subject>Parvoviridae Infections - virology</subject><subject>Plant Sciences</subject><subject>Swine</subject><subject>Swine Diseases - pathology</subject><subject>Swine Diseases - prevention & control</subject><subject>Swine Diseases - virology</subject><subject>Tight junctions</subject><subject>Tight Junctions - virology</subject><subject>Virology</subject><subject>Virus Replication</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU9rFTEUxUNR2mfbD9CNBNy4id78mbzJUh5VKwVdKHQXMpmkzfPN5JnMKO8r-KlNnLZCwVXg3N89OZeD0AWFNxRg_TZTyiQjQFtCGyWJPEIr2qwZUUrcPEMrUAxI20h1gl7kvAWAtmXiGJ1woGvOJV-h35tIwuidnUIc8a8w3eF9TDaMDnfRmp8hzRmbsX9UbUg2LjLDxtfNjIvDzgyDmWI6YHuY4veK7lPs58W4Okzh9m7C23n8K2UcPb76crkhnxi2brfLZ-i5N7vszu_fU_Tt_eXXzUdy_fnD1ebdNbF8zSYifTnDq873HGTPbM-dtJYqaTroqOm8cq4Bp3jrWm9E40XnaS-sULTMheSn6PXiW_L9mF2e9BByTWBGF-esGSgFgjccCvrqCbqNcxpLukq1TDEmKkUXyqaYc3Je71MYTDpoCroWpZeidClK16J0DfHy3nnuBtc_bjw0UwC2ALmMxluX_n39f9c_xX2gOg</recordid><startdate>20181001</startdate><enddate>20181001</enddate><creator>Zhang, Jing</creator><creator>Lu, Yangchao</creator><creator>Li, Shaowen</creator><creator>Ku, Xugang</creator><creator>Liu, Xiaoli</creator><creator>Memon, Atta Muhammad</creator><creator>He, Qigai</creator><creator>Bi, Dingren</creator><creator>Meng, Xianrong</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20181001</creationdate><title>Co-infection with porcine bocavirus and porcine circovirus 2 affects inflammatory cytokine production and tight junctions of IPEC-J2 cells</title><author>Zhang, Jing ; Lu, Yangchao ; Li, Shaowen ; Ku, Xugang ; Liu, Xiaoli ; Memon, Atta Muhammad ; He, Qigai ; Bi, Dingren ; Meng, Xianrong</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-6f008f9bfd306d2cd3e6cc196ab0b1abf9ee50e938e8fa45f4bf1d4c4910b1463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Bocavirus - pathogenicity</topic><topic>Cell Line</topic><topic>Cell proliferation</topic><topic>Circoviridae Infections - prevention & control</topic><topic>Circoviridae Infections - virology</topic><topic>Circovirus - pathogenicity</topic><topic>Coinfection</topic><topic>Cytokines</topic><topic>Cytokines - biosynthesis</topic><topic>Cytopathogenic Effect, Viral</topic><topic>Cytotoxicity</topic><topic>Diarrhea</topic><topic>Enterocytes</topic><topic>Gastrointestinal diseases</topic><topic>Infections</topic><topic>Inflammation</topic><topic>Intestine</topic><topic>Livestock</topic><topic>Medical Microbiology</topic><topic>Parvoviridae Infections - virology</topic><topic>Plant Sciences</topic><topic>Swine</topic><topic>Swine Diseases - pathology</topic><topic>Swine Diseases - prevention & control</topic><topic>Swine Diseases - virology</topic><topic>Tight junctions</topic><topic>Tight Junctions - virology</topic><topic>Virology</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Jing</creatorcontrib><creatorcontrib>Lu, Yangchao</creatorcontrib><creatorcontrib>Li, Shaowen</creatorcontrib><creatorcontrib>Ku, Xugang</creatorcontrib><creatorcontrib>Liu, Xiaoli</creatorcontrib><creatorcontrib>Memon, Atta Muhammad</creatorcontrib><creatorcontrib>He, Qigai</creatorcontrib><creatorcontrib>Bi, Dingren</creatorcontrib><creatorcontrib>Meng, Xianrong</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus genes</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Jing</au><au>Lu, Yangchao</au><au>Li, Shaowen</au><au>Ku, Xugang</au><au>Liu, Xiaoli</au><au>Memon, Atta Muhammad</au><au>He, Qigai</au><au>Bi, Dingren</au><au>Meng, Xianrong</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Co-infection with porcine bocavirus and porcine circovirus 2 affects inflammatory cytokine production and tight junctions of IPEC-J2 cells</atitle><jtitle>Virus genes</jtitle><stitle>Virus Genes</stitle><addtitle>Virus Genes</addtitle><date>2018-10-01</date><risdate>2018</risdate><volume>54</volume><issue>5</issue><spage>684</spage><epage>693</epage><pages>684-693</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>Porcine bocavirus (PBoV) has a high prevalence in both healthy and diseased swine around the world. It was recently reported that PBoV and porcine circovirus type 2 (PCV2)—which contribute to porcine diarrheal disease—have a high rate of co-infection. To clarify the pathogenesis of PBoV, we examined the co-infection rate and effects of these two pathogens in IPEC-J2 porcine intestinal enterocytes. Both single and co-infection had cytopathic effects in IPEC-J2 cells. The apoptosis and proliferation rates of cells infected with both viruses did not differ significantly from those of cells infected with either one alone. PBoV and PCV2 induced the upregulation of inflammatory cytokines and the downregulation of the tight junction proteins occludin and claudin 1 in the early stage of infection, leading to destruction of epithelial barrier integrity and enhanced cytotoxicity. These findings provide insight into the pathogenic mechanisms of PBoV and PCV2 and a basis for developing effective strategies to prevent the spread of gastrointestinal diseases in pigs and other livestock.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30173363</pmid><doi>10.1007/s11262-018-1596-6</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0920-8569 |
| ispartof | Virus genes, 2018-10, Vol.54 (5), p.684-693 |
| issn | 0920-8569 1572-994X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2099043530 |
| source | MEDLINE; SpringerNature Journals |
| subjects | Animals Apoptosis Biomedical and Life Sciences Biomedicine Bocavirus - pathogenicity Cell Line Cell proliferation Circoviridae Infections - prevention & control Circoviridae Infections - virology Circovirus - pathogenicity Coinfection Cytokines Cytokines - biosynthesis Cytopathogenic Effect, Viral Cytotoxicity Diarrhea Enterocytes Gastrointestinal diseases Infections Inflammation Intestine Livestock Medical Microbiology Parvoviridae Infections - virology Plant Sciences Swine Swine Diseases - pathology Swine Diseases - prevention & control Swine Diseases - virology Tight junctions Tight Junctions - virology Virology Virus Replication |
| title | Co-infection with porcine bocavirus and porcine circovirus 2 affects inflammatory cytokine production and tight junctions of IPEC-J2 cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T06%3A56%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Co-infection%20with%20porcine%20bocavirus%20and%20porcine%20circovirus%202%20affects%20inflammatory%20cytokine%20production%20and%20tight%20junctions%20of%20IPEC-J2%20cells&rft.jtitle=Virus%20genes&rft.au=Zhang,%20Jing&rft.date=2018-10-01&rft.volume=54&rft.issue=5&rft.spage=684&rft.epage=693&rft.pages=684-693&rft.issn=0920-8569&rft.eissn=1572-994X&rft_id=info:doi/10.1007/s11262-018-1596-6&rft_dat=%3Cproquest_cross%3E2098292240%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2098292240&rft_id=info:pmid/30173363&rfr_iscdi=true |