Discovery and preclinical validation of proteomic biomarkers in saliva for early detection of oral squamous cell carcinomas
Objectives Our aim was to identify and prevalidate a set of salivary proteins that can distinguish oral squamous cell carcinomas (OSCC) patients from healthy individuals and patients with oral potentially malignant disorders (OPMD). Subjects and methods Proteomes of 60 saliva samples from healthy in...
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| Veröffentlicht in: | Oral diseases 2019-01, Vol.25 (1), p.97-107 |
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| creator | Shan, Jing Sun, Zhida Yang, Jingjing Xu, Juanyong Shi, Wei Wu, You Fan, Yuan Li, Huaiqi |
| description | Objectives
Our aim was to identify and prevalidate a set of salivary proteins that can distinguish oral squamous cell carcinomas (OSCC) patients from healthy individuals and patients with oral potentially malignant disorders (OPMD).
Subjects and methods
Proteomes of 60 saliva samples from healthy individuals, OPMD patients, and OSCC patients were assayed using the isobaric tags for relative and absolute quantitation (iTRAQ) method. Enzyme‐linked immunosorbent assay (ELISA) was used to prevalidate the candidate biomarkers in an independent sample set (n = 90).
Results
In total, 246 differentially expressed proteins were identified by comparing each two groups, and 21 proteins were differentially expressed when OSCC was compared with both OPMD and Control. Three proteins, namely, solute carrier family 3 member 2 (SLC3A2), S100 calcium‐binding protein A2 (S100A2), and interleukin‐1 receptor antagonist protein (IL1RN), were selected as candidate biomarkers. Comparing the OSCC group with the healthy group, the area under curve (AUC) of the three combined biomarkers was 0.89, with a sensitivity of 83.33% and a specificity of 83.33%. Comparing the OSCC group with the OPMD group, the AUC value was 0.87, with a sensitivity of 93.33% and a specificity of 70.00%.
Conclusion
Our study indicates that salivary proteomics is promising for the discovery of OSCC biomarkers. |
| doi_str_mv | 10.1111/odi.12971 |
| format | Article |
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Our aim was to identify and prevalidate a set of salivary proteins that can distinguish oral squamous cell carcinomas (OSCC) patients from healthy individuals and patients with oral potentially malignant disorders (OPMD).
Subjects and methods
Proteomes of 60 saliva samples from healthy individuals, OPMD patients, and OSCC patients were assayed using the isobaric tags for relative and absolute quantitation (iTRAQ) method. Enzyme‐linked immunosorbent assay (ELISA) was used to prevalidate the candidate biomarkers in an independent sample set (n = 90).
Results
In total, 246 differentially expressed proteins were identified by comparing each two groups, and 21 proteins were differentially expressed when OSCC was compared with both OPMD and Control. Three proteins, namely, solute carrier family 3 member 2 (SLC3A2), S100 calcium‐binding protein A2 (S100A2), and interleukin‐1 receptor antagonist protein (IL1RN), were selected as candidate biomarkers. Comparing the OSCC group with the healthy group, the area under curve (AUC) of the three combined biomarkers was 0.89, with a sensitivity of 83.33% and a specificity of 83.33%. Comparing the OSCC group with the OPMD group, the AUC value was 0.87, with a sensitivity of 93.33% and a specificity of 70.00%.
Conclusion
Our study indicates that salivary proteomics is promising for the discovery of OSCC biomarkers.</description><identifier>ISSN: 1354-523X</identifier><identifier>EISSN: 1601-0825</identifier><identifier>DOI: 10.1111/odi.12971</identifier><identifier>PMID: 30169911</identifier><language>eng</language><publisher>Denmark: Wiley Subscription Services, Inc</publisher><subject>Adult ; Aged ; Biomarkers ; Biomarkers, Tumor - analysis ; Calcium-binding protein ; Carcinoma, Squamous Cell - diagnosis ; Dentistry ; Early Detection of Cancer ; Enzyme-linked immunosorbent assay ; Female ; Humans ; Interleukin 1 ; Male ; Middle Aged ; Mouth Neoplasms - diagnosis ; Oral cancer ; Oral carcinoma ; oral potentially malignant disorders ; Oral squamous cell carcinoma ; Proteins ; Proteomics ; Quantitation ; Saliva ; Saliva - chemistry ; saliva proteomics ; Sensitivity and Specificity ; Squamous cell carcinoma</subject><ispartof>Oral diseases, 2019-01, Vol.25 (1), p.97-107</ispartof><rights>2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved</rights><rights>2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. All rights reserved.</rights><rights>Copyright © 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4541-9a26b0f3cf7de8997155038bb537eab9bb52250ced914fe9d37d9fc8c249e9553</citedby><cites>FETCH-LOGICAL-c4541-9a26b0f3cf7de8997155038bb537eab9bb52250ced914fe9d37d9fc8c249e9553</cites><orcidid>0000-0002-4759-5625</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fodi.12971$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fodi.12971$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30169911$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shan, Jing</creatorcontrib><creatorcontrib>Sun, Zhida</creatorcontrib><creatorcontrib>Yang, Jingjing</creatorcontrib><creatorcontrib>Xu, Juanyong</creatorcontrib><creatorcontrib>Shi, Wei</creatorcontrib><creatorcontrib>Wu, You</creatorcontrib><creatorcontrib>Fan, Yuan</creatorcontrib><creatorcontrib>Li, Huaiqi</creatorcontrib><title>Discovery and preclinical validation of proteomic biomarkers in saliva for early detection of oral squamous cell carcinomas</title><title>Oral diseases</title><addtitle>Oral Dis</addtitle><description>Objectives
Our aim was to identify and prevalidate a set of salivary proteins that can distinguish oral squamous cell carcinomas (OSCC) patients from healthy individuals and patients with oral potentially malignant disorders (OPMD).
Subjects and methods
Proteomes of 60 saliva samples from healthy individuals, OPMD patients, and OSCC patients were assayed using the isobaric tags for relative and absolute quantitation (iTRAQ) method. Enzyme‐linked immunosorbent assay (ELISA) was used to prevalidate the candidate biomarkers in an independent sample set (n = 90).
Results
In total, 246 differentially expressed proteins were identified by comparing each two groups, and 21 proteins were differentially expressed when OSCC was compared with both OPMD and Control. Three proteins, namely, solute carrier family 3 member 2 (SLC3A2), S100 calcium‐binding protein A2 (S100A2), and interleukin‐1 receptor antagonist protein (IL1RN), were selected as candidate biomarkers. Comparing the OSCC group with the healthy group, the area under curve (AUC) of the three combined biomarkers was 0.89, with a sensitivity of 83.33% and a specificity of 83.33%. Comparing the OSCC group with the OPMD group, the AUC value was 0.87, with a sensitivity of 93.33% and a specificity of 70.00%.
Conclusion
Our study indicates that salivary proteomics is promising for the discovery of OSCC biomarkers.</description><subject>Adult</subject><subject>Aged</subject><subject>Biomarkers</subject><subject>Biomarkers, Tumor - analysis</subject><subject>Calcium-binding protein</subject><subject>Carcinoma, Squamous Cell - diagnosis</subject><subject>Dentistry</subject><subject>Early Detection of Cancer</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Female</subject><subject>Humans</subject><subject>Interleukin 1</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mouth Neoplasms - diagnosis</subject><subject>Oral cancer</subject><subject>Oral carcinoma</subject><subject>oral potentially malignant disorders</subject><subject>Oral squamous cell carcinoma</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Quantitation</subject><subject>Saliva</subject><subject>Saliva - chemistry</subject><subject>saliva proteomics</subject><subject>Sensitivity and Specificity</subject><subject>Squamous cell carcinoma</subject><issn>1354-523X</issn><issn>1601-0825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1LxDAQhoMofh_8AxLwoodqPpptcxS_YWEvCt5Cmkwh2jaabFcW_7yjqx4Ec8nAPHmYyUvIAWenHM9Z9OGUC13xNbLNJ4wXrBZqHWupykIJ-bhFdnJ-YoxXWopNsiUZn2jN-TZ5vwzZxQWkJbWDpy8JXBeG4GxHF7YL3s5DHGhssRPnEPvgaBNib9MzpEzDQDNSC0vbmCjY1C2phzm4n1cxoSi_jraPY6YOuo46m1wYUJH3yEZruwz73_cuebi-ur-4Laazm7uL82nhSlXyQlsxaVgrXVt5qDWuqRSTddMoWYFtNBZCKObAa162oL2svG5d7USpQSsld8nxyos7vI6Q56bHpXEWOwCOZQTTdTWpheSIHv1Bn-KYBpzOCK5KITSvPoUnK8qlmHOC1rykgH-yNJyZz0QMJmK-EkH28Ns4Nj34X_InAgTOVsBb6GD5v8nMLu9Wyg87xpbH</recordid><startdate>201901</startdate><enddate>201901</enddate><creator>Shan, Jing</creator><creator>Sun, Zhida</creator><creator>Yang, Jingjing</creator><creator>Xu, Juanyong</creator><creator>Shi, Wei</creator><creator>Wu, You</creator><creator>Fan, Yuan</creator><creator>Li, Huaiqi</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-4759-5625</orcidid></search><sort><creationdate>201901</creationdate><title>Discovery and preclinical validation of proteomic biomarkers in saliva for early detection of oral squamous cell carcinomas</title><author>Shan, Jing ; Sun, Zhida ; Yang, Jingjing ; Xu, Juanyong ; Shi, Wei ; Wu, You ; Fan, Yuan ; Li, Huaiqi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4541-9a26b0f3cf7de8997155038bb537eab9bb52250ced914fe9d37d9fc8c249e9553</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biomarkers</topic><topic>Biomarkers, Tumor - analysis</topic><topic>Calcium-binding protein</topic><topic>Carcinoma, Squamous Cell - diagnosis</topic><topic>Dentistry</topic><topic>Early Detection of Cancer</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Female</topic><topic>Humans</topic><topic>Interleukin 1</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mouth Neoplasms - diagnosis</topic><topic>Oral cancer</topic><topic>Oral carcinoma</topic><topic>oral potentially malignant disorders</topic><topic>Oral squamous cell carcinoma</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Quantitation</topic><topic>Saliva</topic><topic>Saliva - chemistry</topic><topic>saliva proteomics</topic><topic>Sensitivity and Specificity</topic><topic>Squamous cell carcinoma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shan, Jing</creatorcontrib><creatorcontrib>Sun, Zhida</creatorcontrib><creatorcontrib>Yang, Jingjing</creatorcontrib><creatorcontrib>Xu, Juanyong</creatorcontrib><creatorcontrib>Shi, Wei</creatorcontrib><creatorcontrib>Wu, You</creatorcontrib><creatorcontrib>Fan, Yuan</creatorcontrib><creatorcontrib>Li, Huaiqi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Oral diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shan, Jing</au><au>Sun, Zhida</au><au>Yang, Jingjing</au><au>Xu, Juanyong</au><au>Shi, Wei</au><au>Wu, You</au><au>Fan, Yuan</au><au>Li, Huaiqi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Discovery and preclinical validation of proteomic biomarkers in saliva for early detection of oral squamous cell carcinomas</atitle><jtitle>Oral diseases</jtitle><addtitle>Oral Dis</addtitle><date>2019-01</date><risdate>2019</risdate><volume>25</volume><issue>1</issue><spage>97</spage><epage>107</epage><pages>97-107</pages><issn>1354-523X</issn><eissn>1601-0825</eissn><abstract>Objectives
Our aim was to identify and prevalidate a set of salivary proteins that can distinguish oral squamous cell carcinomas (OSCC) patients from healthy individuals and patients with oral potentially malignant disorders (OPMD).
Subjects and methods
Proteomes of 60 saliva samples from healthy individuals, OPMD patients, and OSCC patients were assayed using the isobaric tags for relative and absolute quantitation (iTRAQ) method. Enzyme‐linked immunosorbent assay (ELISA) was used to prevalidate the candidate biomarkers in an independent sample set (n = 90).
Results
In total, 246 differentially expressed proteins were identified by comparing each two groups, and 21 proteins were differentially expressed when OSCC was compared with both OPMD and Control. Three proteins, namely, solute carrier family 3 member 2 (SLC3A2), S100 calcium‐binding protein A2 (S100A2), and interleukin‐1 receptor antagonist protein (IL1RN), were selected as candidate biomarkers. Comparing the OSCC group with the healthy group, the area under curve (AUC) of the three combined biomarkers was 0.89, with a sensitivity of 83.33% and a specificity of 83.33%. Comparing the OSCC group with the OPMD group, the AUC value was 0.87, with a sensitivity of 93.33% and a specificity of 70.00%.
Conclusion
Our study indicates that salivary proteomics is promising for the discovery of OSCC biomarkers.</abstract><cop>Denmark</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30169911</pmid><doi>10.1111/odi.12971</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-4759-5625</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Oral diseases, 2019-01, Vol.25 (1), p.97-107 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Adult Aged Biomarkers Biomarkers, Tumor - analysis Calcium-binding protein Carcinoma, Squamous Cell - diagnosis Dentistry Early Detection of Cancer Enzyme-linked immunosorbent assay Female Humans Interleukin 1 Male Middle Aged Mouth Neoplasms - diagnosis Oral cancer Oral carcinoma oral potentially malignant disorders Oral squamous cell carcinoma Proteins Proteomics Quantitation Saliva Saliva - chemistry saliva proteomics Sensitivity and Specificity Squamous cell carcinoma |
| title | Discovery and preclinical validation of proteomic biomarkers in saliva for early detection of oral squamous cell carcinomas |
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