Ameliorative effects of Matricaria chamomilla L. hydroalcoholic extract on scopolamine-induced memory impairment in rats: A behavioral and molecular study
Matricaria chamomilla L. is a medicinal herb traditionally used as the anti-inflammatory, antimicrobial, antiviral, anxiolytic and antidepressant agent. Nevertheless, supporting evidence demonstrated its memory enhancing activity and antioxidant properties. To investigate the effects of the hydroalc...
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Veröffentlicht in: | Phytomedicine (Stuttgart) 2018-08, Vol.47, p.113-120 |
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description | Matricaria chamomilla L. is a medicinal herb traditionally used as the anti-inflammatory, antimicrobial, antiviral, anxiolytic and antidepressant agent. Nevertheless, supporting evidence demonstrated its memory enhancing activity and antioxidant properties.
To investigate the effects of the hydroalcoholic extract of M. chamomilla L. on memory processes in a scopolamine-induced a rat model of amnesia and to reveal its underlying mechanism of action.
The hydroalcoholic extract (25 and 75 mg/kg) was intraperitoneally administered to rats once daily for 7 days, and scopolamine (0.7 mg/kg) was injected 30 min before the behavioral testing to induce memory impairment. The phytochemical composition of the extract was quantified by HPLC/DAD analysis. Y-maze and radial arm-maze tests were employed for memory assessing. Acetylcholinesterase activity was measured in the rat hippocampus. Superoxide dismutase, glutathione peroxidase, and catalase specific activities along with the total content of reduced glutathione and protein carbonyl and malondialdehyde levels were also measured in the rat hippocampus. qRT-PCR was used to quantify BDNF mRNA and IL1β mRNA expression in the rat hippocampus.
We first identified the chlorogenic acid, apigenin-7-glucoside, rutin, cynaroside, luteolin, apigenin and derivatives of apigenin-7-glucoside as the extract major components. Furthermore, we showed that the extract reversed the scopolamine-induced decreasing of the spontaneous alternation in the Y-maze test and the scopolamine-induced increasing of the working and reference memory errors in the radial arm maze test. Also, the scopolamine-induced alteration of the acetylcholinesterase activity and the oxidant-antioxidant balance in the rat hippocampus was recovered by the treatment with the extract. Finally, we demonstrated that the extract restored the scopolamine-decreased BDNF expression and increased IL1β expression in the rat hippocampus.
These findings suggest that the extract could be a potent neuropharmacological agent against amnesia via modulating cholinergic activity, neuroinflammation and promoting antioxidant action in the rat hippocampus.
[Display omitted] |
doi_str_mv | 10.1016/j.phymed.2018.04.049 |
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To investigate the effects of the hydroalcoholic extract of M. chamomilla L. on memory processes in a scopolamine-induced a rat model of amnesia and to reveal its underlying mechanism of action.
The hydroalcoholic extract (25 and 75 mg/kg) was intraperitoneally administered to rats once daily for 7 days, and scopolamine (0.7 mg/kg) was injected 30 min before the behavioral testing to induce memory impairment. The phytochemical composition of the extract was quantified by HPLC/DAD analysis. Y-maze and radial arm-maze tests were employed for memory assessing. Acetylcholinesterase activity was measured in the rat hippocampus. Superoxide dismutase, glutathione peroxidase, and catalase specific activities along with the total content of reduced glutathione and protein carbonyl and malondialdehyde levels were also measured in the rat hippocampus. qRT-PCR was used to quantify BDNF mRNA and IL1β mRNA expression in the rat hippocampus.
We first identified the chlorogenic acid, apigenin-7-glucoside, rutin, cynaroside, luteolin, apigenin and derivatives of apigenin-7-glucoside as the extract major components. Furthermore, we showed that the extract reversed the scopolamine-induced decreasing of the spontaneous alternation in the Y-maze test and the scopolamine-induced increasing of the working and reference memory errors in the radial arm maze test. Also, the scopolamine-induced alteration of the acetylcholinesterase activity and the oxidant-antioxidant balance in the rat hippocampus was recovered by the treatment with the extract. Finally, we demonstrated that the extract restored the scopolamine-decreased BDNF expression and increased IL1β expression in the rat hippocampus.
These findings suggest that the extract could be a potent neuropharmacological agent against amnesia via modulating cholinergic activity, neuroinflammation and promoting antioxidant action in the rat hippocampus.
[Display omitted]</description><identifier>ISSN: 0944-7113</identifier><identifier>EISSN: 1618-095X</identifier><identifier>DOI: 10.1016/j.phymed.2018.04.049</identifier><identifier>PMID: 30166095</identifier><language>eng</language><publisher>Germany: Elsevier GmbH</publisher><subject>Alzheimer's disease ; Amnesia - drug therapy ; Animals ; Antioxidants - pharmacology ; Cognition - drug effects ; Glutathione - metabolism ; Glutathione Peroxidase - metabolism ; Hippocampus - drug effects ; Male ; Malondialdehyde - metabolism ; Matricaria - chemistry ; Matricaria chamomilla extract ; Maze Learning - drug effects ; Memory ; Memory - drug effects ; Memory Disorders - chemically induced ; Memory Disorders - drug therapy ; Oxidative stress ; Oxidative Stress - drug effects ; Plant Extracts - pharmacology ; Rats ; Scopolamine ; Scopolamine - adverse effects ; Superoxide Dismutase - metabolism</subject><ispartof>Phytomedicine (Stuttgart), 2018-08, Vol.47, p.113-120</ispartof><rights>2018 Elsevier GmbH</rights><rights>Copyright © 2018 Elsevier GmbH. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-5eda481081754c1369a0d877ce2ffc9b88cefcd39bd548e2f4a9a447bdeae7df3</citedby><cites>FETCH-LOGICAL-c362t-5eda481081754c1369a0d877ce2ffc9b88cefcd39bd548e2f4a9a447bdeae7df3</cites><orcidid>0000-0001-6981-4174</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.phymed.2018.04.049$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30166095$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ionita, Radu</creatorcontrib><creatorcontrib>Postu, Paula Alexandra</creatorcontrib><creatorcontrib>Mihasan, Marius</creatorcontrib><creatorcontrib>Gorgan, Dragos Lucian</creatorcontrib><creatorcontrib>Hancianu, Monica</creatorcontrib><creatorcontrib>Cioanca, Oana</creatorcontrib><creatorcontrib>Hritcu, Lucian</creatorcontrib><title>Ameliorative effects of Matricaria chamomilla L. hydroalcoholic extract on scopolamine-induced memory impairment in rats: A behavioral and molecular study</title><title>Phytomedicine (Stuttgart)</title><addtitle>Phytomedicine</addtitle><description>Matricaria chamomilla L. is a medicinal herb traditionally used as the anti-inflammatory, antimicrobial, antiviral, anxiolytic and antidepressant agent. Nevertheless, supporting evidence demonstrated its memory enhancing activity and antioxidant properties.
To investigate the effects of the hydroalcoholic extract of M. chamomilla L. on memory processes in a scopolamine-induced a rat model of amnesia and to reveal its underlying mechanism of action.
The hydroalcoholic extract (25 and 75 mg/kg) was intraperitoneally administered to rats once daily for 7 days, and scopolamine (0.7 mg/kg) was injected 30 min before the behavioral testing to induce memory impairment. The phytochemical composition of the extract was quantified by HPLC/DAD analysis. Y-maze and radial arm-maze tests were employed for memory assessing. Acetylcholinesterase activity was measured in the rat hippocampus. Superoxide dismutase, glutathione peroxidase, and catalase specific activities along with the total content of reduced glutathione and protein carbonyl and malondialdehyde levels were also measured in the rat hippocampus. qRT-PCR was used to quantify BDNF mRNA and IL1β mRNA expression in the rat hippocampus.
We first identified the chlorogenic acid, apigenin-7-glucoside, rutin, cynaroside, luteolin, apigenin and derivatives of apigenin-7-glucoside as the extract major components. Furthermore, we showed that the extract reversed the scopolamine-induced decreasing of the spontaneous alternation in the Y-maze test and the scopolamine-induced increasing of the working and reference memory errors in the radial arm maze test. Also, the scopolamine-induced alteration of the acetylcholinesterase activity and the oxidant-antioxidant balance in the rat hippocampus was recovered by the treatment with the extract. Finally, we demonstrated that the extract restored the scopolamine-decreased BDNF expression and increased IL1β expression in the rat hippocampus.
These findings suggest that the extract could be a potent neuropharmacological agent against amnesia via modulating cholinergic activity, neuroinflammation and promoting antioxidant action in the rat hippocampus.
[Display omitted]</description><subject>Alzheimer's disease</subject><subject>Amnesia - drug therapy</subject><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Cognition - drug effects</subject><subject>Glutathione - metabolism</subject><subject>Glutathione Peroxidase - metabolism</subject><subject>Hippocampus - drug effects</subject><subject>Male</subject><subject>Malondialdehyde - metabolism</subject><subject>Matricaria - chemistry</subject><subject>Matricaria chamomilla extract</subject><subject>Maze Learning - drug effects</subject><subject>Memory</subject><subject>Memory - drug effects</subject><subject>Memory Disorders - chemically induced</subject><subject>Memory Disorders - drug therapy</subject><subject>Oxidative stress</subject><subject>Oxidative Stress - drug effects</subject><subject>Plant Extracts - pharmacology</subject><subject>Rats</subject><subject>Scopolamine</subject><subject>Scopolamine - adverse effects</subject><subject>Superoxide Dismutase - metabolism</subject><issn>0944-7113</issn><issn>1618-095X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc-KFDEQh4Mo7rj6BiI5euk2mU7_iQdhWNRdGPGi4C1UJ9V0hqQzJt2D_So-rWlm9yoUBJKv6kflI-QtZyVnvPlwKs_j6tGUe8a7kolc8hnZ8YZ3BZP1r-dkx6QQRct5dUNepXRijAvZspfkpsoDmgztyN-DR2dDhNlekOIwoJ4TDQP9BnO0GqIFqkfwwVvngB5LOq4mBnA6jMFZTfHPHEHPNEw06XAODrydsLCTWTQa6tGHuFLrz2Cjx2mmdqI5LX2kB9rjCJct3FGYMhsc6sVBpGlezPqavBjAJXzzeN6Sn18-_7i7L47fvz7cHY6Frpr9XNRoQHScdbytheZVI4GZrm017odBy77rNA7aVLI3tejypQAJQrS9QcDWDNUteX-de47h94JpVt4mjXndCcOS1J7Jrm1qJmVGxRXVMaQUcVDnaD3EVXGmNivqpK5W1GZFMZFra3v3mLD029tT05OGDHy6Apj3vFiMKmmLU_5AG7MQZYL9f8I_IXWk9A</recordid><startdate>20180801</startdate><enddate>20180801</enddate><creator>Ionita, Radu</creator><creator>Postu, Paula Alexandra</creator><creator>Mihasan, Marius</creator><creator>Gorgan, Dragos Lucian</creator><creator>Hancianu, Monica</creator><creator>Cioanca, Oana</creator><creator>Hritcu, Lucian</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6981-4174</orcidid></search><sort><creationdate>20180801</creationdate><title>Ameliorative effects of Matricaria chamomilla L. hydroalcoholic extract on scopolamine-induced memory impairment in rats: A behavioral and molecular study</title><author>Ionita, Radu ; Postu, Paula Alexandra ; Mihasan, Marius ; Gorgan, Dragos Lucian ; Hancianu, Monica ; Cioanca, Oana ; Hritcu, Lucian</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-5eda481081754c1369a0d877ce2ffc9b88cefcd39bd548e2f4a9a447bdeae7df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Alzheimer's disease</topic><topic>Amnesia - drug therapy</topic><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Cognition - drug effects</topic><topic>Glutathione - metabolism</topic><topic>Glutathione Peroxidase - metabolism</topic><topic>Hippocampus - drug effects</topic><topic>Male</topic><topic>Malondialdehyde - metabolism</topic><topic>Matricaria - chemistry</topic><topic>Matricaria chamomilla extract</topic><topic>Maze Learning - drug effects</topic><topic>Memory</topic><topic>Memory - drug effects</topic><topic>Memory Disorders - chemically induced</topic><topic>Memory Disorders - drug therapy</topic><topic>Oxidative stress</topic><topic>Oxidative Stress - drug effects</topic><topic>Plant Extracts - pharmacology</topic><topic>Rats</topic><topic>Scopolamine</topic><topic>Scopolamine - adverse effects</topic><topic>Superoxide Dismutase - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ionita, Radu</creatorcontrib><creatorcontrib>Postu, Paula Alexandra</creatorcontrib><creatorcontrib>Mihasan, Marius</creatorcontrib><creatorcontrib>Gorgan, Dragos Lucian</creatorcontrib><creatorcontrib>Hancianu, Monica</creatorcontrib><creatorcontrib>Cioanca, Oana</creatorcontrib><creatorcontrib>Hritcu, Lucian</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Phytomedicine (Stuttgart)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ionita, Radu</au><au>Postu, Paula Alexandra</au><au>Mihasan, Marius</au><au>Gorgan, Dragos Lucian</au><au>Hancianu, Monica</au><au>Cioanca, Oana</au><au>Hritcu, Lucian</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Ameliorative effects of Matricaria chamomilla L. hydroalcoholic extract on scopolamine-induced memory impairment in rats: A behavioral and molecular study</atitle><jtitle>Phytomedicine (Stuttgart)</jtitle><addtitle>Phytomedicine</addtitle><date>2018-08-01</date><risdate>2018</risdate><volume>47</volume><spage>113</spage><epage>120</epage><pages>113-120</pages><issn>0944-7113</issn><eissn>1618-095X</eissn><abstract>Matricaria chamomilla L. is a medicinal herb traditionally used as the anti-inflammatory, antimicrobial, antiviral, anxiolytic and antidepressant agent. Nevertheless, supporting evidence demonstrated its memory enhancing activity and antioxidant properties.
To investigate the effects of the hydroalcoholic extract of M. chamomilla L. on memory processes in a scopolamine-induced a rat model of amnesia and to reveal its underlying mechanism of action.
The hydroalcoholic extract (25 and 75 mg/kg) was intraperitoneally administered to rats once daily for 7 days, and scopolamine (0.7 mg/kg) was injected 30 min before the behavioral testing to induce memory impairment. The phytochemical composition of the extract was quantified by HPLC/DAD analysis. Y-maze and radial arm-maze tests were employed for memory assessing. Acetylcholinesterase activity was measured in the rat hippocampus. Superoxide dismutase, glutathione peroxidase, and catalase specific activities along with the total content of reduced glutathione and protein carbonyl and malondialdehyde levels were also measured in the rat hippocampus. qRT-PCR was used to quantify BDNF mRNA and IL1β mRNA expression in the rat hippocampus.
We first identified the chlorogenic acid, apigenin-7-glucoside, rutin, cynaroside, luteolin, apigenin and derivatives of apigenin-7-glucoside as the extract major components. Furthermore, we showed that the extract reversed the scopolamine-induced decreasing of the spontaneous alternation in the Y-maze test and the scopolamine-induced increasing of the working and reference memory errors in the radial arm maze test. Also, the scopolamine-induced alteration of the acetylcholinesterase activity and the oxidant-antioxidant balance in the rat hippocampus was recovered by the treatment with the extract. Finally, we demonstrated that the extract restored the scopolamine-decreased BDNF expression and increased IL1β expression in the rat hippocampus.
These findings suggest that the extract could be a potent neuropharmacological agent against amnesia via modulating cholinergic activity, neuroinflammation and promoting antioxidant action in the rat hippocampus.
[Display omitted]</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>30166095</pmid><doi>10.1016/j.phymed.2018.04.049</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-6981-4174</orcidid></addata></record> |
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subjects | Alzheimer's disease Amnesia - drug therapy Animals Antioxidants - pharmacology Cognition - drug effects Glutathione - metabolism Glutathione Peroxidase - metabolism Hippocampus - drug effects Male Malondialdehyde - metabolism Matricaria - chemistry Matricaria chamomilla extract Maze Learning - drug effects Memory Memory - drug effects Memory Disorders - chemically induced Memory Disorders - drug therapy Oxidative stress Oxidative Stress - drug effects Plant Extracts - pharmacology Rats Scopolamine Scopolamine - adverse effects Superoxide Dismutase - metabolism |
title | Ameliorative effects of Matricaria chamomilla L. hydroalcoholic extract on scopolamine-induced memory impairment in rats: A behavioral and molecular study |
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