MiR‐324‐5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8
Objectives The purpose of this study was to further clarify the role and underlying mechanism of miR‐324‐5p in gastric cancer. Methods The expressions of miR‐324‐5p and TSPAN8 as determined by qRT‐PCR or Western blot were compared between the gastric cancer tissues and normal tissues. Human gastric...
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| Veröffentlicht in: | Journal of pharmacy and pharmacology 2018-11, Vol.70 (11), p.1513-1520 |
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| creator | Lin, Hai Zhou, Ai‐Jun Zhang, Jing‐Yu Liu, Shu‐Fang Gu, Jian‐Xiang |
| description | Objectives
The purpose of this study was to further clarify the role and underlying mechanism of miR‐324‐5p in gastric cancer.
Methods
The expressions of miR‐324‐5p and TSPAN8 as determined by qRT‐PCR or Western blot were compared between the gastric cancer tissues and normal tissues. Human gastric cancer cell line SGC‐7901 was cultured and transfected with miR‐324‐5p mimic/inhibitor or pcDNA‐TSPAN8. The cell survival was assessed by the cell viability and apoptosis. Luciferase reporter gene assays were performed to explore the interaction between miR‐324‐5p and TSPAN8 in SGC‐7901 cells.
Key findings
MiR‐324‐5p was decreased in human gastric carcinoma tissues (n = 33), but TSPAN8 protein expression was increased in the gastric carcinoma tissues (n = 33). Moreover, miR‐324‐5p inhibited the viability and induced the apoptosis of gastric cancer cells in vitro. TSPAN8 is a functional target of miR‐324‐5p in gastric cancer. MiR‐324‐5p was further confirmed to reduce gastric cancer cell viability and induce apoptosis via downregulating TSPAN8 in SGC‐7901 cells in vitro. Additionally, miR‐324‐5p overexpression markedly inhibited the tumorigenesis of gastric cancer cells in vivo, as shown by the smaller tumour volume compared with the control.
Conclusions
This study suggested a novel, probable mechanism of miR‐324‐5p in gastric cancer context and revealed that miR‐324‐5p inhibited gastric cancer cell survival by targeting TSPAN8. |
| doi_str_mv | 10.1111/jphp.12995 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2097595833</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2097595833</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3575-84f07f9342906bde813ec503cf7d43051fee2c2f9c8105b14c6dc65e7802b37f3</originalsourceid><addsrcrecordid>eNp9kM1O3DAURi3UipkCGx6gstQNqhS4tuM4Xo5QKa0ojGBYW47jTDzKX-0ENLs-Qp-xT9JMM7Dogru4V7o6-vTpIHRK4JyMc7Hpyu6cUCn5AZpTiGkkCE_foTkApRHjgs3QhxA2ACCSJDlEMwaESwkwR-UPd__n129G43HzDnubD8YG_OR05irXb7Fucuya6au7tuvb4ML4wWsdeu8MNrox1mNjqyrgvvTtsC5x3eZDpXvXrPHqYbm4TY_R-0JXwZ7s7xF6vPqyuryObu6-frtc3ERmLMqjNC5AFJLFVEKS5TYlzBoOzBQijxlwUlhLDS2kSQnwjMQmyU3CrUiBZkwU7AidTbmdb38ONvSqdmHXTTe2HYKiIAWXPGVsRD_9h27awTdjO0UJEYQmROyozxNlfBuCt4XqvKu13yoCaudf7fyrf_5H-OM-cshqm7-iL8JHgEzAs6vs9o0o9X15vZxC_wKTkZDT</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2117126173</pqid></control><display><type>article</type><title>MiR‐324‐5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Lin, Hai ; Zhou, Ai‐Jun ; Zhang, Jing‐Yu ; Liu, Shu‐Fang ; Gu, Jian‐Xiang</creator><creatorcontrib>Lin, Hai ; Zhou, Ai‐Jun ; Zhang, Jing‐Yu ; Liu, Shu‐Fang ; Gu, Jian‐Xiang</creatorcontrib><description>Objectives
The purpose of this study was to further clarify the role and underlying mechanism of miR‐324‐5p in gastric cancer.
Methods
The expressions of miR‐324‐5p and TSPAN8 as determined by qRT‐PCR or Western blot were compared between the gastric cancer tissues and normal tissues. Human gastric cancer cell line SGC‐7901 was cultured and transfected with miR‐324‐5p mimic/inhibitor or pcDNA‐TSPAN8. The cell survival was assessed by the cell viability and apoptosis. Luciferase reporter gene assays were performed to explore the interaction between miR‐324‐5p and TSPAN8 in SGC‐7901 cells.
Key findings
MiR‐324‐5p was decreased in human gastric carcinoma tissues (n = 33), but TSPAN8 protein expression was increased in the gastric carcinoma tissues (n = 33). Moreover, miR‐324‐5p inhibited the viability and induced the apoptosis of gastric cancer cells in vitro. TSPAN8 is a functional target of miR‐324‐5p in gastric cancer. MiR‐324‐5p was further confirmed to reduce gastric cancer cell viability and induce apoptosis via downregulating TSPAN8 in SGC‐7901 cells in vitro. Additionally, miR‐324‐5p overexpression markedly inhibited the tumorigenesis of gastric cancer cells in vivo, as shown by the smaller tumour volume compared with the control.
Conclusions
This study suggested a novel, probable mechanism of miR‐324‐5p in gastric cancer context and revealed that miR‐324‐5p inhibited gastric cancer cell survival by targeting TSPAN8.</description><identifier>ISSN: 0022-3573</identifier><identifier>EISSN: 2042-7158</identifier><identifier>DOI: 10.1111/jphp.12995</identifier><identifier>PMID: 30159900</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>Animals ; Apoptosis ; Apoptosis - drug effects ; Cell Line, Tumor ; Cell survival ; Female ; Gastric cancer ; Gene Expression Regulation, Neoplastic ; Humans ; Mice, Inbred BALB C ; Mice, Nude ; MicroRNAs - genetics ; MicroRNAs - metabolism ; MiR‐324‐5p ; Reporter gene ; Signal Transduction ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology ; Tetraspanins - genetics ; Tetraspanins - metabolism ; Tissues ; TSPAN8 ; Tumor Burden ; Tumorigenesis ; Tumors ; viability</subject><ispartof>Journal of pharmacy and pharmacology, 2018-11, Vol.70 (11), p.1513-1520</ispartof><rights>2018 Royal Pharmaceutical Society</rights><rights>2018 Royal Pharmaceutical Society.</rights><rights>Copyright © 2018 Royal Pharmaceutical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3575-84f07f9342906bde813ec503cf7d43051fee2c2f9c8105b14c6dc65e7802b37f3</citedby><cites>FETCH-LOGICAL-c3575-84f07f9342906bde813ec503cf7d43051fee2c2f9c8105b14c6dc65e7802b37f3</cites><orcidid>0000-0003-2251-7062</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjphp.12995$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjphp.12995$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30159900$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Hai</creatorcontrib><creatorcontrib>Zhou, Ai‐Jun</creatorcontrib><creatorcontrib>Zhang, Jing‐Yu</creatorcontrib><creatorcontrib>Liu, Shu‐Fang</creatorcontrib><creatorcontrib>Gu, Jian‐Xiang</creatorcontrib><title>MiR‐324‐5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8</title><title>Journal of pharmacy and pharmacology</title><addtitle>J Pharm Pharmacol</addtitle><description>Objectives
The purpose of this study was to further clarify the role and underlying mechanism of miR‐324‐5p in gastric cancer.
Methods
The expressions of miR‐324‐5p and TSPAN8 as determined by qRT‐PCR or Western blot were compared between the gastric cancer tissues and normal tissues. Human gastric cancer cell line SGC‐7901 was cultured and transfected with miR‐324‐5p mimic/inhibitor or pcDNA‐TSPAN8. The cell survival was assessed by the cell viability and apoptosis. Luciferase reporter gene assays were performed to explore the interaction between miR‐324‐5p and TSPAN8 in SGC‐7901 cells.
Key findings
MiR‐324‐5p was decreased in human gastric carcinoma tissues (n = 33), but TSPAN8 protein expression was increased in the gastric carcinoma tissues (n = 33). Moreover, miR‐324‐5p inhibited the viability and induced the apoptosis of gastric cancer cells in vitro. TSPAN8 is a functional target of miR‐324‐5p in gastric cancer. MiR‐324‐5p was further confirmed to reduce gastric cancer cell viability and induce apoptosis via downregulating TSPAN8 in SGC‐7901 cells in vitro. Additionally, miR‐324‐5p overexpression markedly inhibited the tumorigenesis of gastric cancer cells in vivo, as shown by the smaller tumour volume compared with the control.
Conclusions
This study suggested a novel, probable mechanism of miR‐324‐5p in gastric cancer context and revealed that miR‐324‐5p inhibited gastric cancer cell survival by targeting TSPAN8.</description><subject>Animals</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Cell Line, Tumor</subject><subject>Cell survival</subject><subject>Female</subject><subject>Gastric cancer</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Humans</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Nude</subject><subject>MicroRNAs - genetics</subject><subject>MicroRNAs - metabolism</subject><subject>MiR‐324‐5p</subject><subject>Reporter gene</subject><subject>Signal Transduction</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><subject>Tetraspanins - genetics</subject><subject>Tetraspanins - metabolism</subject><subject>Tissues</subject><subject>TSPAN8</subject><subject>Tumor Burden</subject><subject>Tumorigenesis</subject><subject>Tumors</subject><subject>viability</subject><issn>0022-3573</issn><issn>2042-7158</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kM1O3DAURi3UipkCGx6gstQNqhS4tuM4Xo5QKa0ojGBYW47jTDzKX-0ENLs-Qp-xT9JMM7Dogru4V7o6-vTpIHRK4JyMc7Hpyu6cUCn5AZpTiGkkCE_foTkApRHjgs3QhxA2ACCSJDlEMwaESwkwR-UPd__n129G43HzDnubD8YG_OR05irXb7Fucuya6au7tuvb4ML4wWsdeu8MNrox1mNjqyrgvvTtsC5x3eZDpXvXrPHqYbm4TY_R-0JXwZ7s7xF6vPqyuryObu6-frtc3ERmLMqjNC5AFJLFVEKS5TYlzBoOzBQijxlwUlhLDS2kSQnwjMQmyU3CrUiBZkwU7AidTbmdb38ONvSqdmHXTTe2HYKiIAWXPGVsRD_9h27awTdjO0UJEYQmROyozxNlfBuCt4XqvKu13yoCaudf7fyrf_5H-OM-cshqm7-iL8JHgEzAs6vs9o0o9X15vZxC_wKTkZDT</recordid><startdate>201811</startdate><enddate>201811</enddate><creator>Lin, Hai</creator><creator>Zhou, Ai‐Jun</creator><creator>Zhang, Jing‐Yu</creator><creator>Liu, Shu‐Fang</creator><creator>Gu, Jian‐Xiang</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7TK</scope><scope>7U9</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-2251-7062</orcidid></search><sort><creationdate>201811</creationdate><title>MiR‐324‐5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8</title><author>Lin, Hai ; Zhou, Ai‐Jun ; Zhang, Jing‐Yu ; Liu, Shu‐Fang ; Gu, Jian‐Xiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3575-84f07f9342906bde813ec503cf7d43051fee2c2f9c8105b14c6dc65e7802b37f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Cell Line, Tumor</topic><topic>Cell survival</topic><topic>Female</topic><topic>Gastric cancer</topic><topic>Gene Expression Regulation, Neoplastic</topic><topic>Humans</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Nude</topic><topic>MicroRNAs - genetics</topic><topic>MicroRNAs - metabolism</topic><topic>MiR‐324‐5p</topic><topic>Reporter gene</topic><topic>Signal Transduction</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><topic>Tetraspanins - genetics</topic><topic>Tetraspanins - metabolism</topic><topic>Tissues</topic><topic>TSPAN8</topic><topic>Tumor Burden</topic><topic>Tumorigenesis</topic><topic>Tumors</topic><topic>viability</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Hai</creatorcontrib><creatorcontrib>Zhou, Ai‐Jun</creatorcontrib><creatorcontrib>Zhang, Jing‐Yu</creatorcontrib><creatorcontrib>Liu, Shu‐Fang</creatorcontrib><creatorcontrib>Gu, Jian‐Xiang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of pharmacy and pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Hai</au><au>Zhou, Ai‐Jun</au><au>Zhang, Jing‐Yu</au><au>Liu, Shu‐Fang</au><au>Gu, Jian‐Xiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>MiR‐324‐5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8</atitle><jtitle>Journal of pharmacy and pharmacology</jtitle><addtitle>J Pharm Pharmacol</addtitle><date>2018-11</date><risdate>2018</risdate><volume>70</volume><issue>11</issue><spage>1513</spage><epage>1520</epage><pages>1513-1520</pages><issn>0022-3573</issn><eissn>2042-7158</eissn><abstract>Objectives
The purpose of this study was to further clarify the role and underlying mechanism of miR‐324‐5p in gastric cancer.
Methods
The expressions of miR‐324‐5p and TSPAN8 as determined by qRT‐PCR or Western blot were compared between the gastric cancer tissues and normal tissues. Human gastric cancer cell line SGC‐7901 was cultured and transfected with miR‐324‐5p mimic/inhibitor or pcDNA‐TSPAN8. The cell survival was assessed by the cell viability and apoptosis. Luciferase reporter gene assays were performed to explore the interaction between miR‐324‐5p and TSPAN8 in SGC‐7901 cells.
Key findings
MiR‐324‐5p was decreased in human gastric carcinoma tissues (n = 33), but TSPAN8 protein expression was increased in the gastric carcinoma tissues (n = 33). Moreover, miR‐324‐5p inhibited the viability and induced the apoptosis of gastric cancer cells in vitro. TSPAN8 is a functional target of miR‐324‐5p in gastric cancer. MiR‐324‐5p was further confirmed to reduce gastric cancer cell viability and induce apoptosis via downregulating TSPAN8 in SGC‐7901 cells in vitro. Additionally, miR‐324‐5p overexpression markedly inhibited the tumorigenesis of gastric cancer cells in vivo, as shown by the smaller tumour volume compared with the control.
Conclusions
This study suggested a novel, probable mechanism of miR‐324‐5p in gastric cancer context and revealed that miR‐324‐5p inhibited gastric cancer cell survival by targeting TSPAN8.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30159900</pmid><doi>10.1111/jphp.12995</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-2251-7062</orcidid></addata></record> |
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| ispartof | Journal of pharmacy and pharmacology, 2018-11, Vol.70 (11), p.1513-1520 |
| issn | 0022-3573 2042-7158 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2097595833 |
| source | Wiley Online Library - AutoHoldings Journals; MEDLINE; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Apoptosis Apoptosis - drug effects Cell Line, Tumor Cell survival Female Gastric cancer Gene Expression Regulation, Neoplastic Humans Mice, Inbred BALB C Mice, Nude MicroRNAs - genetics MicroRNAs - metabolism MiR‐324‐5p Reporter gene Signal Transduction Stomach Neoplasms - genetics Stomach Neoplasms - metabolism Stomach Neoplasms - pathology Tetraspanins - genetics Tetraspanins - metabolism Tissues TSPAN8 Tumor Burden Tumorigenesis Tumors viability |
| title | MiR‐324‐5p reduces viability and induces apoptosis in gastric cancer cells through modulating TSPAN8 |
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