Antibacterial activity of disulfiram and its metabolites
Aims Disulfiram (Antabuse™) and its metabolites formed in vivo were evaluated as antibacterial agents against thirty species of Gram‐positive and Gram‐negative bacteria. The synergistic potential of disulfiram (DSF) and metabolite diethyldithiocarbamate (DDTC) with approved antibiotics were also com...
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| Veröffentlicht in: | Journal of applied microbiology 2019-01, Vol.126 (1), p.79-86 |
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| container_title | Journal of applied microbiology |
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| creator | Frazier, K.R. Moore, J.A. Long, T.E. |
| description | Aims
Disulfiram (Antabuse™) and its metabolites formed in vivo were evaluated as antibacterial agents against thirty species of Gram‐positive and Gram‐negative bacteria. The synergistic potential of disulfiram (DSF) and metabolite diethyldithiocarbamate (DDTC) with approved antibiotics were also compared by isobologram (checkerboard) analysis.
Methods and Results
Standard microdilution susceptibility testing showed that most DSF metabolites did not possess appreciable antibacterial activity except for DDTC in Bacillus anthracis. Checkerboard studies revealed similarities between the combination drug effects of DSF and DDTC with standard antibiotics.
Conclusions
It was concluded from the susceptibility data that the metabolites would not extend the antibacterial spectrum of DSF in vivo. The data also suggest that the DDTC by‐product of DSF metabolism potentiates the antibacterial activity of DSF as both a standalone and combination agent.
Significance and Impact of the Study
The study provides a greater understanding of the antibacterial effects of Antabuse and its metabolites. This research also demonstrates the potential application of DSF as an antibiotic adjuvant for the treatment of resistant staph infections. |
| doi_str_mv | 10.1111/jam.14094 |
| format | Article |
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Disulfiram (Antabuse™) and its metabolites formed in vivo were evaluated as antibacterial agents against thirty species of Gram‐positive and Gram‐negative bacteria. The synergistic potential of disulfiram (DSF) and metabolite diethyldithiocarbamate (DDTC) with approved antibiotics were also compared by isobologram (checkerboard) analysis.
Methods and Results
Standard microdilution susceptibility testing showed that most DSF metabolites did not possess appreciable antibacterial activity except for DDTC in Bacillus anthracis. Checkerboard studies revealed similarities between the combination drug effects of DSF and DDTC with standard antibiotics.
Conclusions
It was concluded from the susceptibility data that the metabolites would not extend the antibacterial spectrum of DSF in vivo. The data also suggest that the DDTC by‐product of DSF metabolism potentiates the antibacterial activity of DSF as both a standalone and combination agent.
Significance and Impact of the Study
The study provides a greater understanding of the antibacterial effects of Antabuse and its metabolites. This research also demonstrates the potential application of DSF as an antibiotic adjuvant for the treatment of resistant staph infections.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14094</identifier><identifier>PMID: 30160334</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Antibacterial activity ; Antibacterial agents ; Antibiotics ; antimicrobials ; Bacillus ; Data processing ; Disulfiram ; Gram-negative bacteria ; In vivo methods and tests ; Metabolism ; Metabolites ; staphylococci</subject><ispartof>Journal of applied microbiology, 2019-01, Vol.126 (1), p.79-86</ispartof><rights>2018 The Society for Applied Microbiology</rights><rights>2018 The Society for Applied Microbiology.</rights><rights>Copyright © 2019 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4194-4faa6d8736e6cdcb36a54dcd2ce78ed005fdb9a4abcf936b4f6ea26719c4997b3</citedby><cites>FETCH-LOGICAL-c4194-4faa6d8736e6cdcb36a54dcd2ce78ed005fdb9a4abcf936b4f6ea26719c4997b3</cites><orcidid>0000-0003-0213-4726</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14094$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14094$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30160334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frazier, K.R.</creatorcontrib><creatorcontrib>Moore, J.A.</creatorcontrib><creatorcontrib>Long, T.E.</creatorcontrib><title>Antibacterial activity of disulfiram and its metabolites</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
Disulfiram (Antabuse™) and its metabolites formed in vivo were evaluated as antibacterial agents against thirty species of Gram‐positive and Gram‐negative bacteria. The synergistic potential of disulfiram (DSF) and metabolite diethyldithiocarbamate (DDTC) with approved antibiotics were also compared by isobologram (checkerboard) analysis.
Methods and Results
Standard microdilution susceptibility testing showed that most DSF metabolites did not possess appreciable antibacterial activity except for DDTC in Bacillus anthracis. Checkerboard studies revealed similarities between the combination drug effects of DSF and DDTC with standard antibiotics.
Conclusions
It was concluded from the susceptibility data that the metabolites would not extend the antibacterial spectrum of DSF in vivo. The data also suggest that the DDTC by‐product of DSF metabolism potentiates the antibacterial activity of DSF as both a standalone and combination agent.
Significance and Impact of the Study
The study provides a greater understanding of the antibacterial effects of Antabuse and its metabolites. This research also demonstrates the potential application of DSF as an antibiotic adjuvant for the treatment of resistant staph infections.</description><subject>Antibacterial activity</subject><subject>Antibacterial agents</subject><subject>Antibiotics</subject><subject>antimicrobials</subject><subject>Bacillus</subject><subject>Data processing</subject><subject>Disulfiram</subject><subject>Gram-negative bacteria</subject><subject>In vivo methods and tests</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>staphylococci</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kEtLAzEUhYMoVqsL_4AMuNHFtHnPZFmKTypudB3yGkiZR01mlP57Y6e6ELybexYfh8MHwAWCM5RuvlbNDFEo6AE4QYSzHPMCH-4yzRks8AScxriGEBHI-DGYEIg4JISegHLR9l4r07vgVZ2l4D98v826KrM-DnXlg2oy1drM9zFrXK90V_vexTNwVKk6uvP9n4K3u9vX5UO-erl_XC5WuaFI0JxWSnFbFoQ7bqzRhCtGrbHYuKJ0FkJWWS0UVdpUgnBNK-5Umo-EoUIUmkzB9di7Cd374GIvGx-Nq2vVum6IEkNRMMEQwgm9-oOuuyG0aZ3EiBUEl5jSRN2MlAldjMFVchN8o8JWIii_dcqkU-50JvZy3zjoxtlf8sdfAuYj8Olrt_2_ST4tnsfKL6VLfhg</recordid><startdate>201901</startdate><enddate>201901</enddate><creator>Frazier, K.R.</creator><creator>Moore, J.A.</creator><creator>Long, T.E.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0213-4726</orcidid></search><sort><creationdate>201901</creationdate><title>Antibacterial activity of disulfiram and its metabolites</title><author>Frazier, K.R. ; Moore, J.A. ; Long, T.E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4194-4faa6d8736e6cdcb36a54dcd2ce78ed005fdb9a4abcf936b4f6ea26719c4997b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibacterial activity</topic><topic>Antibacterial agents</topic><topic>Antibiotics</topic><topic>antimicrobials</topic><topic>Bacillus</topic><topic>Data processing</topic><topic>Disulfiram</topic><topic>Gram-negative bacteria</topic><topic>In vivo methods and tests</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>staphylococci</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frazier, K.R.</creatorcontrib><creatorcontrib>Moore, J.A.</creatorcontrib><creatorcontrib>Long, T.E.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frazier, K.R.</au><au>Moore, J.A.</au><au>Long, T.E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial activity of disulfiram and its metabolites</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2019-01</date><risdate>2019</risdate><volume>126</volume><issue>1</issue><spage>79</spage><epage>86</epage><pages>79-86</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
Disulfiram (Antabuse™) and its metabolites formed in vivo were evaluated as antibacterial agents against thirty species of Gram‐positive and Gram‐negative bacteria. The synergistic potential of disulfiram (DSF) and metabolite diethyldithiocarbamate (DDTC) with approved antibiotics were also compared by isobologram (checkerboard) analysis.
Methods and Results
Standard microdilution susceptibility testing showed that most DSF metabolites did not possess appreciable antibacterial activity except for DDTC in Bacillus anthracis. Checkerboard studies revealed similarities between the combination drug effects of DSF and DDTC with standard antibiotics.
Conclusions
It was concluded from the susceptibility data that the metabolites would not extend the antibacterial spectrum of DSF in vivo. The data also suggest that the DDTC by‐product of DSF metabolism potentiates the antibacterial activity of DSF as both a standalone and combination agent.
Significance and Impact of the Study
The study provides a greater understanding of the antibacterial effects of Antabuse and its metabolites. This research also demonstrates the potential application of DSF as an antibiotic adjuvant for the treatment of resistant staph infections.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>30160334</pmid><doi>10.1111/jam.14094</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0003-0213-4726</orcidid></addata></record> |
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| ispartof | Journal of applied microbiology, 2019-01, Vol.126 (1), p.79-86 |
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| language | eng |
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| source | Oxford University Press Journals All Titles (1996-Current); Wiley Online Library All Journals |
| subjects | Antibacterial activity Antibacterial agents Antibiotics antimicrobials Bacillus Data processing Disulfiram Gram-negative bacteria In vivo methods and tests Metabolism Metabolites staphylococci |
| title | Antibacterial activity of disulfiram and its metabolites |
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