Switching Antibody Specificity through Minimal Mutation

Antibody 1E9, which was elicited with a hexachloronorbornene derivative and catalyzes the Diels–Alder reaction between tetrachlorothiophene dioxide and N-ethylmaleimide with high efficiency, was successfully reengineered to bind a range of structurally diverse steroids with nanomolar affinities. Rem...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of molecular biology 2008-04, Vol.377 (4), p.993-1001
Hauptverfasser:	Piatesi, Andrea, Aldag, Caroline, Hilvert, Donald
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino Acid Substitution Antibodies, Monoclonal - genetics Antibodies, Monoclonal - metabolism Antibody Specificity Binding Sites, Antibody - genetics complementarity Cross Reactions - genetics cross-reactivity immunoglobulin Models, Biological Models, Molecular molecular recognition Molecular Sequence Data Mutagenesis, Site-Directed - methods Mutant Proteins - metabolism Mutation - physiology Progesterone - immunology Progesterone - metabolism Sequence Homology, Amino Acid steroid Steroids - immunology Steroids - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!