Investigation of manganese(II)-insulin complexes using electrospray ionization mass spectrometry
Manganese is a trace element in human nutrition. It is involved in many enzymes, proteins and biological activities. Mn(II) ion has the capable of binding to protein or peptides. Insulin is a blood glucose-lowering peptide hormone and it is secreted by the pancreatic β-cells. In this study, the bind...
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| Veröffentlicht in: | International journal of biological macromolecules 2018-12, Vol.120 (Pt A), p.557-565 |
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| creator | Gülfen, Mustafa Özdemir, Abdil Lin, Jung-Lee Chen, Chung-Hsuan |
| description | Manganese is a trace element in human nutrition. It is involved in many enzymes, proteins and biological activities. Mn(II) ion has the capable of binding to protein or peptides. Insulin is a blood glucose-lowering peptide hormone and it is secreted by the pancreatic β-cells. In this study, the binding capability of Mn(II) ions to insulin was studied using ESI-MS, nano-ESI-MS and MS/MS methods. The binding of Mn(II) ions to insulin molecule was studied by examining the effect of pH, the molar ratio of Mn(II) ions to insulin, the flow rate with nano-ESI system and MS/MS spectrometry. The ESI-MS measurements showed that the Mn(II)-insulin complexation mostly produces ML and M2L type complexes. The highest binding ratio was found at pH 7. The complex formation equilibrium constants of Mn(II)-insulin were calculated as Kf1: 1.03 ± 0.12 × 104 and Kf2: 1.93 ± 0.17 × 103. The nano-ESI-MS and MS/MS measurements exhibited strong and specific binding of Mn(II) ions to insulin molecule. It was concluded from all the ESI-MS measurements that Mn(II) ion has a high affinity to insulin molecule to form stable complexes.
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| doi_str_mv | 10.1016/j.ijbiomac.2018.08.122 |
| format | Article |
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[Display omitted]</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2018.08.122</identifier><identifier>PMID: 30153462</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Cations, Divalent ; Cattle ; Coordination Complexes - chemistry ; Equilibrium ; Hydrogen-Ion Concentration ; Insulin ; Insulin - chemistry ; Kinetics ; Manganese - chemistry ; Manganese-insulin complexes ; Mass spectrometry ; Protein Binding ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry</subject><ispartof>International journal of biological macromolecules, 2018-12, Vol.120 (Pt A), p.557-565</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-dbdebabc51898dad8af5b1d22fd38afcec34f8a7c0c1c6aa2981611914e56713</citedby><cites>FETCH-LOGICAL-c368t-dbdebabc51898dad8af5b1d22fd38afcec34f8a7c0c1c6aa2981611914e56713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ijbiomac.2018.08.122$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30153462$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gülfen, Mustafa</creatorcontrib><creatorcontrib>Özdemir, Abdil</creatorcontrib><creatorcontrib>Lin, Jung-Lee</creatorcontrib><creatorcontrib>Chen, Chung-Hsuan</creatorcontrib><title>Investigation of manganese(II)-insulin complexes using electrospray ionization mass spectrometry</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>Manganese is a trace element in human nutrition. It is involved in many enzymes, proteins and biological activities. Mn(II) ion has the capable of binding to protein or peptides. Insulin is a blood glucose-lowering peptide hormone and it is secreted by the pancreatic β-cells. In this study, the binding capability of Mn(II) ions to insulin was studied using ESI-MS, nano-ESI-MS and MS/MS methods. The binding of Mn(II) ions to insulin molecule was studied by examining the effect of pH, the molar ratio of Mn(II) ions to insulin, the flow rate with nano-ESI system and MS/MS spectrometry. The ESI-MS measurements showed that the Mn(II)-insulin complexation mostly produces ML and M2L type complexes. The highest binding ratio was found at pH 7. The complex formation equilibrium constants of Mn(II)-insulin were calculated as Kf1: 1.03 ± 0.12 × 104 and Kf2: 1.93 ± 0.17 × 103. The nano-ESI-MS and MS/MS measurements exhibited strong and specific binding of Mn(II) ions to insulin molecule. It was concluded from all the ESI-MS measurements that Mn(II) ion has a high affinity to insulin molecule to form stable complexes.
[Display omitted]</description><subject>Animals</subject><subject>Cations, Divalent</subject><subject>Cattle</subject><subject>Coordination Complexes - chemistry</subject><subject>Equilibrium</subject><subject>Hydrogen-Ion Concentration</subject><subject>Insulin</subject><subject>Insulin - chemistry</subject><subject>Kinetics</subject><subject>Manganese - chemistry</subject><subject>Manganese-insulin complexes</subject><subject>Mass spectrometry</subject><subject>Protein Binding</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem Mass Spectrometry</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EgvL4BZQlLBI8duM6O1DFo1IlNuyNY08qV3lhJxXl63EpZctqrPG9M3MPIddAM6Ag7taZW5eua7TJGAWZUZkBY0dkAnJWpJRSfkwmFKaQSuD0jJyHsI5dkYM8JWecQs6ngk3I-6LdYBjcSg-ua5OuShrdrnSLAW8Wi9vUtWGsXZuYrulr_MSQjMG1qwRrNIPvQu_1NolO97Uf0OgQktD_fDY4-O0lOal0HfDqt16Qt6fHt_lLunx9XswflqnhQg6pLS2WujTxvkJabaWu8hIsY5Xl8W3Q8Gkl9cxQA0ZozQoJAqCAKeZiBvyC3OzH9r77GGMi1bhgsK5jlG4MitFC5DkrOI1SsZeaeH_wWKneu0b7rQKqdnDVWh3gqh1cRaWKcKPx-nfHWDZo_2wHmlFwvxdgDLpx6FUwDluD1vlIRNnO_bfjGz5lkY8</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Gülfen, Mustafa</creator><creator>Özdemir, Abdil</creator><creator>Lin, Jung-Lee</creator><creator>Chen, Chung-Hsuan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201812</creationdate><title>Investigation of manganese(II)-insulin complexes using electrospray ionization mass spectrometry</title><author>Gülfen, Mustafa ; Özdemir, Abdil ; Lin, Jung-Lee ; Chen, Chung-Hsuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-dbdebabc51898dad8af5b1d22fd38afcec34f8a7c0c1c6aa2981611914e56713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Cations, Divalent</topic><topic>Cattle</topic><topic>Coordination Complexes - chemistry</topic><topic>Equilibrium</topic><topic>Hydrogen-Ion Concentration</topic><topic>Insulin</topic><topic>Insulin - chemistry</topic><topic>Kinetics</topic><topic>Manganese - chemistry</topic><topic>Manganese-insulin complexes</topic><topic>Mass spectrometry</topic><topic>Protein Binding</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tandem Mass Spectrometry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gülfen, Mustafa</creatorcontrib><creatorcontrib>Özdemir, Abdil</creatorcontrib><creatorcontrib>Lin, Jung-Lee</creatorcontrib><creatorcontrib>Chen, Chung-Hsuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gülfen, Mustafa</au><au>Özdemir, Abdil</au><au>Lin, Jung-Lee</au><au>Chen, Chung-Hsuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Investigation of manganese(II)-insulin complexes using electrospray ionization mass spectrometry</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2018-12</date><risdate>2018</risdate><volume>120</volume><issue>Pt A</issue><spage>557</spage><epage>565</epage><pages>557-565</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>Manganese is a trace element in human nutrition. It is involved in many enzymes, proteins and biological activities. Mn(II) ion has the capable of binding to protein or peptides. Insulin is a blood glucose-lowering peptide hormone and it is secreted by the pancreatic β-cells. In this study, the binding capability of Mn(II) ions to insulin was studied using ESI-MS, nano-ESI-MS and MS/MS methods. The binding of Mn(II) ions to insulin molecule was studied by examining the effect of pH, the molar ratio of Mn(II) ions to insulin, the flow rate with nano-ESI system and MS/MS spectrometry. The ESI-MS measurements showed that the Mn(II)-insulin complexation mostly produces ML and M2L type complexes. The highest binding ratio was found at pH 7. The complex formation equilibrium constants of Mn(II)-insulin were calculated as Kf1: 1.03 ± 0.12 × 104 and Kf2: 1.93 ± 0.17 × 103. The nano-ESI-MS and MS/MS measurements exhibited strong and specific binding of Mn(II) ions to insulin molecule. It was concluded from all the ESI-MS measurements that Mn(II) ion has a high affinity to insulin molecule to form stable complexes.
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Cations, Divalent Cattle Coordination Complexes - chemistry Equilibrium Hydrogen-Ion Concentration Insulin Insulin - chemistry Kinetics Manganese - chemistry Manganese-insulin complexes Mass spectrometry Protein Binding Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry |
| title | Investigation of manganese(II)-insulin complexes using electrospray ionization mass spectrometry |
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